Restriction map for
pF1KB3452
(Circular) (Six-base) MAPSORT of: pF1KB3452.seq Check: 5690 from: 1 to: 3764
pF1KB3452 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AfeI AGC'GCT
Cuts at: 250 250
Size: 3764
AflII C'TTAA_G
Cuts at: 271 271
Size: 3764
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 255 784 255
Size: 529 3235
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 259 780 1194 1442 1477 259
Size: 521 414 248 35 2546
Fragments arranged by size:
2546 521 414 248 35
BanII G_rGCy'C
Cuts at: 778 1808 778
Size: 1030 2734
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 455 1284 455
Size: 829 2935
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 262 1389 1482 2538 262
Size: 1127 93 1056 1488
Fragments arranged by size:
1488 1127 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 976 1057 1392 3536 976
Size: 81 335 2144 1204
Fragments arranged by size:
2144 1204 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 214
Size: 3764
Bpu10I CC'TnA_GC
Cuts at: 338 338
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 940 1819 2311 2609 673
Size: 267 879 492 298 1828
Fragments arranged by size:
1828 879 492 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 621 1117 621
Size: 496 3268
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 1195 1443 2145 3641 1195
Size: 248 702 1496 1318
Fragments arranged by size:
1496 1318 702 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 746 1189 1947 2524 3020 746
Size: 443 758 577 496 1490
Fragments arranged by size:
1490 758 577 496 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 145
Size: 3764
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 733 778 1556 1746 2538 733
Size: 45 778 190 792 1959
Fragments arranged by size:
1959 792 778 190 45
BsmBI CGTCTCn'nnnn_
Cuts at: 190 190
Size: 3764
Bsp1286I G_dGCh'C
Cuts at: 262 733 778 1389 1482 1556 1746 1808 2538
Size: 471 45 611 93 74 190 62 730
Cuts at: 2538 262
Size: 1488
Fragments arranged by size:
1488 730 611 471 190 93 74 62
45
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 814 1028 1330 1711 814
Size: 214 302 381 2867
Fragments arranged by size:
2867 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 682 1676 682
Size: 994 2770
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstYI r'GATC_y
Cuts at: 1 789 1256 1279 1614 1860 2872 2958 2970
Size: 788 467 23 335 246 1012 86 12
Cuts at: 2970 3316 1
Size: 346 449
Fragments arranged by size:
1012 788 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 344 734 344
Size: 390 3374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 491 763 491
Size: 272 3492
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 416 542 1349 1523 1914 1941 3286 416
Size: 126 807 174 391 27 1345 894
Fragments arranged by size:
1345 894 807 391 174 126 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 1589 2021 2768 3036 214
Size: 1375 432 747 268 942
Fragments arranged by size:
1375 942 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 139 139
Size: 3764
EcoO109I rG'GnC_Cy
Cuts at: 903 903
Size: 3764
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
EcoRV GAT'ATC
Cuts at: 351 351
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 183 252 376 753 1198 1446 2468 3447 183
Size: 69 124 377 445 248 1022 979 500
Fragments arranged by size:
1022 979 500 445 377 248 124 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1606 1636 1638 1668 547
Size: 32 1027 30 2 30 2643
Fragments arranged by size:
2643 1027 32 30 30 2
HincII GTy'rAC
Cuts at: 669 803 669
Size: 134 3630
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 418 544 1525 418
Size: 126 981 2657
Fragments arranged by size:
2657 981 126
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 875 1189 1549 2562 2807 875
Size: 314 360 1013 245 1832
Fragments arranged by size:
1832 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NcoI C'CATG_G
Cuts at: 734 734
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NruI TCG'CGA
Cuts at: 128 128
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PfoI T'CCnGG_A
Cuts at: 191 191
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PmlI CAC'GTG
Cuts at: 621 621
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PstI C_TGCA'G
Cuts at: 811 1496 811
Size: 685 3079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 1189 1549 1189
Size: 360 3404
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 605 807 1492 2485 2676 33
Size: 572 202 685 993 191 1121
Fragments arranged by size:
1121 993 685 572 202 191
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 271 688 919 1834 2326 2588 271
Size: 417 231 915 492 262 1447
Fragments arranged by size:
1447 915 492 417 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 734 898 1252 734
Size: 164 354 3246
Fragments arranged by size:
3246 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PfoI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PflMI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI