Restriction map for
pF1KB3456
(Circular) (Six-base) MAPSORT of: pF1KB3456.seq Check: 4219 from: 1 to: 4190
pF1KB3456 4190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2447 3194 3462 2015
Size: 432 747 268 2743
Fragments arranged by size:
2743 747 432 268
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AleI CACnn'nnGTG
Cuts at: 802 802
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 1495 3062 1495
Size: 1567 2623
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 1194 3543 3799 1194
Size: 2349 256 1585
Fragments arranged by size:
2349 1585 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 953 1210 953
Size: 257 3933
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 1206 1620 1868 1903 1206
Size: 414 248 35 3493
Fragments arranged by size:
3493 414 248 35
BanII G_rGCy'C
Cuts at: 366 465 570 1204 2234 366
Size: 99 105 634 1030 2322
Fragments arranged by size:
2322 1030 634 105 99
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BbsI GAAGACnn'nnnn_
Cuts at: 242 242
Size: 4190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4156
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 521 1710 521
Size: 1189 3001
BglI GCCn_nnn'nGGC
Cuts at: 112 510 1141 112
Size: 398 631 3161
Fragments arranged by size:
3161 631 398
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 444 585 809 1815 1908 2964 444
Size: 141 224 1006 93 1056 1670
Fragments arranged by size:
1670 1056 1006 224 141 93
BmgBI CAC'GTC
Cuts at: 631 631
Size: 4190
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1483 1818 3962 1402
Size: 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 345 165
Size: 180 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 651 1543 651
Size: 892 3298
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1112 1159 1379 1900 2578 2852 2999 3410 1112
Size: 47 220 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 220 147 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 159 189 997 1027 159
Size: 30 808 30 3322
Fragments arranged by size:
3322 808 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 215 765 215
Size: 550 3640
BseYI C'CCAG_C
Cuts at: 678 1615 2373 2950 3446 678
Size: 937 758 577 496 1422
Fragments arranged by size:
1422 937 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 465 1204 1982 2172 2964 465
Size: 739 778 190 792 1691
Fragments arranged by size:
1691 792 778 739 190
BsmI GAATG_Cn'
Cuts at: 541 541
Size: 4190
BsmBI CGTCTCn'nnnn_
Cuts at: 208 208
Size: 4190
Bsp1286I G_dGCh'C
Cuts at: 366 444 465 570 585 809 1204 1815 1908
Size: 78 21 105 15 224 395 611 93
Cuts at: 1908 1982 2172 2234 2964 366
Size: 74 190 62 730 1592
Fragments arranged by size:
1592 730 611 395 224 190 105 93
78 74 62 21 15
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 400 1240 1454 1756 2137 400
Size: 840 214 302 381 2453
Fragments arranged by size:
2453 840 381 302 214
BsrBI CCG'CTC
Cuts at: 50 485 2482 2536 4020 4185 50
Size: 435 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 435 165 55 54
BsrDI GCAATG_nn'
Cuts at: 524 2102 524
Size: 1578 2612
BsrFI r'CCGG_y
Cuts at: 111 2188 2369 2578 111
Size: 2077 181 209 1723
Fragments arranged by size:
2077 1723 209 181
BssSI C'ACGA_G
Cuts at: 569 2461 2819 569
Size: 1892 358 1940
Fragments arranged by size:
1940 1892 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 537 894 537
Size: 357 3833
BstYI r'GATC_y
Cuts at: 1 1215 1682 1705 2040 2286 3298 3384 3396
Size: 1214 467 23 335 246 1012 86 12
Cuts at: 3396 3742 1
Size: 346 449
Fragments arranged by size:
1214 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 1088 1142 1775 1949 2340 2367 3712 1088
Size: 54 633 174 391 27 1345 1566
Fragments arranged by size:
1566 1345 633 391 174 54 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 922 2213 2423 922
Size: 1291 210 2689
Fragments arranged by size:
2689 1291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 463 1202 463
Size: 739 3451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 345 2015 2447 3194 3462 165
Size: 180 1670 432 747 268 893
Fragments arranged by size:
1670 893 747 432 268 180
EcoO109I rG'GnC_Cy
Cuts at: 186 1329 186
Size: 1143 3047
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
EcoRV GAT'ATC
Cuts at: 135 925 135
Size: 790 3400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 670 1971 670
Size: 1301 2889
HaeII r_GCGC'y
Cuts at: 175 1624 1872 2894 3873 175
Size: 1449 248 1022 979 492
Fragments arranged by size:
1449 1022 979 492 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 2032 2062 2064 2094 121
Size: 32 1879 30 2 30 2217
Fragments arranged by size:
2217 1879 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 191 2861 3045 191
Size: 2670 184 1336
Fragments arranged by size:
2670 1336 184
MscI TGG'CCA
Cuts at: 1144 1951 1144
Size: 807 3383
MslI CAynn'nnrTG
Cuts at: 340 802 2179 2306 340
Size: 462 1377 127 2224
Fragments arranged by size:
2224 1377 462 127
MspA1I CmG'CkG
Cuts at: 682 1049 1301 1615 1975 2988 3233 682
Size: 367 252 314 360 1013 245 1639
Fragments arranged by size:
1639 1013 367 360 314 252 245
NaeI GCC'GGC
Cuts at: 113 2371 113
Size: 2258 1932
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NgoMIV G'CCGG_C
Cuts at: 111 2369 111
Size: 2258 1932
NspI r_CATG'y
Cuts at: 610 1243 1743 2650 610
Size: 633 500 907 2150
Fragments arranged by size:
2150 907 633 500
PasI CC'CwG_GG
Cuts at: 444 444
Size: 4190
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PmlI CAC'GTG
Cuts at: 651 651
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PpuMI rG'GwC_Cy
Cuts at: 186 186
Size: 4190
PstI C_TGCA'G
Cuts at: 128 194 1237 1922 128
Size: 66 1043 685 2396
Fragments arranged by size:
2396 1043 685 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 682 1615 1975 682
Size: 933 360 2897
Fragments arranged by size:
2897 933 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 465 1204 465
Size: 739 3451
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 124 190 1233 1918 2911 3102 33
Size: 91 66 1043 685 993 191 1121
Fragments arranged by size:
1121 1043 993 685 191 91 66
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 955 1212 955
Size: 257 3933
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 308 1127 1324 1678 308
Size: 819 197 354 2820
Fragments arranged by size:
2820 819 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 692 976 1087 1773 1906 2365 2574 692
Size: 284 111 686 133 459 209 2308
Fragments arranged by size:
2308 686 459 284 209 133 111
TatI w'GTAC_w
Cuts at: 3705 3705
Size: 4190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 369 401 3707 3739 369
Size: 32 3306 32 820
Fragments arranged by size:
3306 820 32 32
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 953 1210 953
Size: 257 3933
XmnI GAAnn'nnTTC
Cuts at: 830 830
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsgI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI