Restriction map for
pF1KB3461
(Circular) (Six-base) MAPSORT of: pF1KB3461.seq Check: 8548 from: 1 to: 3758
pF1KB3461 3758 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 704 796 704
Size: 92 3666
Acc65I G'GTAC_C
Cuts at: 774 774
Size: 3758
AclI AA'CG_TT
Cuts at: 752 3598 752
Size: 2846 912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1583 2015 2762 3030 445
Size: 1138 432 747 268 1173
Fragments arranged by size:
1173 1138 747 432 268
AflIII A'CryG_T
Cuts at: 1110 2214 3191 1110
Size: 1104 977 1677
Fragments arranged by size:
1677 1104 977
AgeI A'CCGG_T
Cuts at: 2146 2146
Size: 3758
AlwNI CAG_nnn'CTG
Cuts at: 1063 2630 1063
Size: 1567 2191
ApaLI G'TGCA_C
Cuts at: 2528 2528
Size: 3758
ApoI r'AATT_y
Cuts at: 247 681 762 3111 3367 247
Size: 434 81 2349 256 638
Fragments arranged by size:
2349 638 434 256 81
AseI AT'TA_AT
Cuts at: 20 20
Size: 3758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
AvaI C'yCGr_G
Cuts at: 778 778
Size: 3758
BamHI G'GATC_C
Cuts at: 783 783
Size: 3758
BanI G'GyrC_C
Cuts at: 774 1188 1436 1471 774
Size: 414 248 35 3061
Fragments arranged by size:
3061 414 248 35
BanII G_rGCy'C
Cuts at: 772 1802 772
Size: 1030 2728
BbeI G_GCGC'C
Cuts at: 1192 1440 1192
Size: 248 3510
BciVI GTATCCnnnnn_n'
Cuts at: 324 1656 2417 3758 324
Size: 1332 761 1341 324
Fragments arranged by size:
1341 1332 761 324
BclI T'GATC_A
Cuts at: 1278 1278
Size: 3758
BglII A'GATC_T
Cuts at: 1273 3310 1273
Size: 2037 1721
BlpI GC'TnA_GC
Cuts at: 870 870
Size: 3758
Bme1580I G_kGCm'C
Cuts at: 1383 1476 2532 1383
Size: 93 1056 2609
Fragments arranged by size:
2609 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 970 1051 1386 3530 970
Size: 81 335 2144 1198
Fragments arranged by size:
2144 1198 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 3758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1813 2305 2603 934
Size: 879 492 298 2089
Fragments arranged by size:
2089 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3462 3462
Size: 3758
BsaAI yAC'GTr
Cuts at: 1111 1111
Size: 3758
BsaBI GATnn'nnATC
Cuts at: 6 1272 3309 6
Size: 1266 2037 455
Fragments arranged by size:
2037 1266 455
BsaHI Gr'CG_yC
Cuts at: 1189 1437 2139 3635 1189
Size: 248 702 1496 1312
Fragments arranged by size:
1496 1312 702 248
BsaWI w'CCGG_w
Cuts at: 947 1468 2146 2420 2567 2978 947
Size: 521 678 274 147 411 1727
Fragments arranged by size:
1727 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 395 436 713 395
Size: 41 277 3440
Fragments arranged by size:
3440 277 41
BseYI C'CCAG_C
Cuts at: 1183 1941 2518 3014 1183
Size: 758 577 496 1927
Fragments arranged by size:
1927 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1300 1346 2554 104
Size: 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 387 772 1550 1740 2532 387
Size: 385 778 190 792 1613
Fragments arranged by size:
1613 792 778 385 190
Bsp1286I G_dGCh'C
Cuts at: 387 772 1383 1476 1550 1740 1802 2532 387
Size: 385 611 93 74 190 62 730 1613
Fragments arranged by size:
1613 730 611 385 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2978 2978
Size: 3758
BspHI T'CATG_A
Cuts at: 243 2934 243
Size: 2691 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1022 1324 1705 808
Size: 214 302 381 2861
Fragments arranged by size:
2861 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2050 2104 3588 3753 50
Size: 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1670 1670
Size: 3758
BsrFI r'CCGG_y
Cuts at: 1756 1937 2146 1756
Size: 181 209 3368
Fragments arranged by size:
3368 209 181
BssSI C'ACGA_G
Cuts at: 2029 2387 2029
Size: 358 3400
BstAPI GCAn_nnn'nTGC
Cuts at: 685 1135 685
Size: 450 3308
BstBI TT'CG_AA
Cuts at: 2119 2119
Size: 3758
BstYI r'GATC_y
Cuts at: 1 783 1250 1273 1608 1854 2866 2952 2964
Size: 782 467 23 335 246 1012 86 12
Cuts at: 2964 3310 1
Size: 346 449
Fragments arranged by size:
1012 782 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3758
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1708 1889 2052 1708
Size: 181 163 3414
Fragments arranged by size:
3414 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 1001
Size: 3758
DraI TTT'AAA
Cuts at: 757 757
Size: 3758
DrdI GACnn_nn'nnGTC
Cuts at: 1464 2322 1464
Size: 858 2900
EaeI y'GGCC_r
Cuts at: 1343 1517 1908 1935 3280 1343
Size: 174 391 27 1345 1821
Fragments arranged by size:
1821 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1343 1343
Size: 3758
EarI CTCTTCn'nnn_
Cuts at: 690 1781 1991 690
Size: 1091 210 2457
Fragments arranged by size:
2457 1091 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1966 2276 2422 3568 1966
Size: 310 146 1146 2156
Fragments arranged by size:
2156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 770 770
Size: 3758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1583 2015 2762 3030 445
Size: 1138 432 747 268 1173
Fragments arranged by size:
1173 1138 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 897 897
Size: 3758
EcoRI G'AATT_C
Cuts at: 762 762
Size: 3758
EcoRV GAT'ATC
Cuts at: 411 411
Size: 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1777 1777
Size: 3758
FspI TGC'GCA
Cuts at: 1539 1539
Size: 3758
HaeII r_GCGC'y
Cuts at: 1192 1440 2462 3441 1192
Size: 248 1022 979 1509
Fragments arranged by size:
1509 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1600 1630 1632 1662 1600
Size: 30 2 30 3696
Fragments arranged by size:
3696 30 30 2
HincII GTy'rAC
Cuts at: 797 797
Size: 3758
KasI G'GCGC_C
Cuts at: 1188 1436 1188
Size: 248 3510
KpnI G_GTAC'C
Cuts at: 778 778
Size: 3758
MluI A'CGCG_T
Cuts at: 3191 3191
Size: 3758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2613 2429
Size: 184 3574
MscI TGG'CCA
Cuts at: 1519 1519
Size: 3758
MslI CAynn'nnrTG
Cuts at: 1747 1874 1747
Size: 127 3631
MspA1I CmG'CkG
Cuts at: 196 337 869 1183 1543 2556 2801 196
Size: 141 532 314 360 1013 245 1153
Fragments arranged by size:
1153 1013 532 360 314 245 141
NaeI GCC'GGC
Cuts at: 1939 1939
Size: 3758
NarI GG'CG_CC
Cuts at: 1189 1437 1189
Size: 248 3510
NcoI C'CATG_G
Cuts at: 107 107
Size: 3758
NgoMIV G'CCGG_C
Cuts at: 1937 1937
Size: 3758
NspI r_CATG'y
Cuts at: 811 1311 2218 811
Size: 500 907 2351
Fragments arranged by size:
2351 907 500
PciI A'CATG_T
Cuts at: 2214 2214
Size: 3758
PflMI CCAn_nnn'nTGG
Cuts at: 148 148
Size: 3758
PfoI T'CCnGG_A
Cuts at: 542 672 542
Size: 130 3628
PmeI GTTT'AAAC
Cuts at: 757 757
Size: 3758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 3726
PstI C_TGCA'G
Cuts at: 805 1490 805
Size: 685 3073
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3758
PvuII CAG'CTG
Cuts at: 337 1183 1543 337
Size: 846 360 2552
Fragments arranged by size:
2552 846 360
RsrII CG'GwC_CG
Cuts at: 1953 1953
Size: 3758
SacI G_AGCT'C
Cuts at: 772 772
Size: 3758
SalI G'TCGA_C
Cuts at: 795 795
Size: 3758
SapI GCTCTTCn'nnn_
Cuts at: 1781 1991 1781
Size: 210 3548
SbfI CC_TGCA'GG
Cuts at: 805 805
Size: 3758
ScaI AGT'ACT
Cuts at: 179 3275 179
Size: 3096 662
SfcI C'TryA_G
Cuts at: 33 308 375 801 1486 2479 2670 33
Size: 275 67 426 685 993 191 1121
Fragments arranged by size:
1121 993 685 426 275 191 67
SfoI GGC'GCC
Cuts at: 1190 1438 1190
Size: 248 3510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3758
SmaI CCC'GGG
Cuts at: 780 780
Size: 3758
SmlI C'TyrA_G
Cuts at: 913 1828 2320 2582 913
Size: 915 492 262 2089
Fragments arranged by size:
2089 915 492 262
SpeI A'CTAG_T
Cuts at: 67 174 67
Size: 107 3651
SphI G_CATG'C
Cuts at: 811 1311 811
Size: 500 3258
SspI AAT'ATT
Cuts at: 626 626
Size: 3758
StyI C'CwwG_G
Cuts at: 107 892 1246 107
Size: 785 354 2619
Fragments arranged by size:
2619 785 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1341 1474 1933 2142 1341
Size: 133 459 209 2957
Fragments arranged by size:
2957 459 209 133
TatI w'GTAC_w
Cuts at: 177 371 3273 177
Size: 194 2902 662
Fragments arranged by size:
2902 662 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 3726
Tth111I GACn'n_nGTC
Cuts at: 1555 1555
Size: 3758
XbaI T'CTAG_A
Cuts at: 789 789
Size: 3758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1253 1253
Size: 3758
XmaI C'CCGG_G
Cuts at: 778 778
Size: 3758
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI