Restriction map for
pF1KB3463
(Circular) (Six-base) MAPSORT of: pF1KB3463.seq Check: 2923 from: 1 to: 7916
pF1KB3463 7916 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 4954 4954
Size: 7916
Acc65I G'GTAC_C
Cuts at: 3523 4932 3523
Size: 1409 6507
AclI AA'CG_TT
Cuts at: 7756 7756
Size: 7916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 2327 2888 3637 3773 4031 4331 4517 5741
Size: 1899 561 749 136 258 300 186 1224
Cuts at: 5741 6173 6526 7188 428
Size: 432 353 662 1156
Fragments arranged by size:
1899 1224 1156 749 662 561 432 353
300 258 186 136
AflII C'TTAA_G
Cuts at: 1515 1515
Size: 7916
AflIII A'CryG_T
Cuts at: 769 4430 5268 7068 7349 769
Size: 3661 838 1800 281 1336
Fragments arranged by size:
3661 1800 1336 838 281
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 7916
AhdI GACnn_n'nnGTC
Cuts at: 3888 3957 3888
Size: 69 7847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3406 3406
Size: 7916
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2935 2967 4194 4226 2935
Size: 32 1227 32 6625
Fragments arranged by size:
6625 1227 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3999 4009 5221 6659 3999
Size: 10 1212 1438 5256
Fragments arranged by size:
5256 1438 1212 10
ApaLI G'TGCA_C
Cuts at: 6754 6754
Size: 7916
ApoI r'AATT_y
Cuts at: 503 1116 1241 1609 1895 2455 2570 2781 2858
Size: 613 125 368 286 560 115 211 77
Cuts at: 2858 3330 3537 4920 7269 7525 503
Size: 472 207 1383 2349 256 894
Fragments arranged by size:
2349 1383 894 613 560 472 368 286
256 211 207 125 115 77
AseI AT'TA_AT
Cuts at: 20 250 20
Size: 230 7686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7916
AvaI C'yCGr_G
Cuts at: 160 172 4936 160
Size: 12 4764 3140
Fragments arranged by size:
4764 3140 12
BamHI G'GATC_C
Cuts at: 1413 4941 1413
Size: 3528 4388
BanI G'GyrC_C
Cuts at: 3523 3657 4932 5346 5594 5629 3523
Size: 134 1275 414 248 35 5810
Fragments arranged by size:
5810 1275 414 248 134 35
BanII G_rGCy'C
Cuts at: 4337 4930 5960 4337
Size: 593 1030 6293
Fragments arranged by size:
6293 1030 593
BbeI G_GCGC'C
Cuts at: 5350 5598 5350
Size: 248 7668
BbsI GAAGACnn'nnnn_
Cuts at: 659 766 1939 2541 3788 659
Size: 107 1173 602 1247 4787
Fragments arranged by size:
4787 1247 1173 602 107
BciVI GTATCCnnnnn_n'
Cuts at: 5814 6870 7916 5814
Size: 1056 1046 5814
Fragments arranged by size:
5814 1056 1046
BclI T'GATC_A
Cuts at: 1355 1833 2355 5436 1355
Size: 478 522 3081 3835
Fragments arranged by size:
3835 3081 522 478
BfrBI ATG'CAT
Cuts at: 627 1173 1386 4734 627
Size: 546 213 3348 3809
Fragments arranged by size:
3809 3348 546 213
BglI GCCn_nnn'nGGC
Cuts at: 3427 4821 3427
Size: 1394 6522
BglII A'GATC_T
Cuts at: 855 3051 5431 7468 855
Size: 2196 2380 2037 1303
Fragments arranged by size:
2380 2196 2037 1303
BlpI GC'TnA_GC
Cuts at: 5028 5028
Size: 7916
Bme1580I G_kGCm'C
Cuts at: 3378 5541 5634 6758 3378
Size: 2163 93 1124 4536
Fragments arranged by size:
4536 2163 1124 93
BmgBI CAC'GTC
Cuts at: 772 772
Size: 7916
BmrI ACTGGGnnnn_n'
Cuts at: 4028 4522 5128 5209 5544 7688 4028
Size: 494 606 81 335 2144 4256
Fragments arranged by size:
4256 2144 606 494 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1071 6283 1071
Size: 5212 2704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1040 3563 3882 4398 4616 4679 943
Size: 97 2523 319 516 218 63 4180
Fragments arranged by size:
4180 2523 516 319 218 97 63
Bpu10I CC'TnA_GC
Cuts at: 1370 2200 1370
Size: 830 7086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 3818 4139 5092 5971 6685 6983 2627
Size: 1191 321 953 879 714 298 3560
Fragments arranged by size:
3560 1191 953 879 714 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 7620 7620
Size: 7916
BsaAI yAC'GTr
Cuts at: 4433 5269 4433
Size: 836 7080
BsaBI GATnn'nnATC
Cuts at: 6 728 5430 7467 6
Size: 722 4702 2037 455
Fragments arranged by size:
4702 2037 722 455
BsaHI Gr'CG_yC
Cuts at: 3799 5347 5595 6297 7793 3799
Size: 1548 248 702 1496 3922
Fragments arranged by size:
3922 1548 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1325 1652 2467 5105 5626 6304 6715 6862 7136
Size: 327 815 2638 521 678 411 147 274
Cuts at: 7136 1325
Size: 2105
Fragments arranged by size:
2638 2105 815 678 521 411 327 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1280 1310 1280
Size: 30 7886
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3493 3821 4401 4619 3493
Size: 328 580 218 6790
Fragments arranged by size:
6790 580 328 218
BseYI C'CCAG_C
Cuts at: 1223 2705 3285 4259 4482 5341 6099 6764 7172
Size: 1482 580 974 223 859 758 665 408
Cuts at: 7172 1223
Size: 1967
Fragments arranged by size:
1967 1482 974 859 758 665 580 408
223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3695 4735 3695
Size: 1040 6876
BsiEI CG_ry'CG
Cuts at: 104 5458 5504 6734 104
Size: 5354 46 1230 1286
Fragments arranged by size:
5354 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 267 3363 4043 4480 4930 5708 5898 6758 267
Size: 3096 680 437 450 778 190 860 1425
Fragments arranged by size:
3096 1425 860 778 680 450 437 190
BsmI GAATG_Cn'
Cuts at: 471 471
Size: 7916
BsmBI CGTCTCn'nnnn_
Cuts at: 1381 1381
Size: 7916
Bsp1286I G_dGCh'C
Cuts at: 267 3363 3378 4043 4337 4480 4930 5541 5634
Size: 3096 15 665 294 143 450 611 93
Cuts at: 5634 5708 5898 5960 6758 267
Size: 74 190 62 798 1425
Fragments arranged by size:
3096 1425 798 665 611 450 294 190
143 93 74 62 15
BspHI T'CATG_A
Cuts at: 2451 6348 2451
Size: 3897 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 894 4966 5180 5482 5863 894
Size: 4072 214 302 381 2947
Fragments arranged by size:
4072 2947 381 302 214
BsrBI CCG'CTC
Cuts at: 50 165 6208 6262 7746 7911 50
Size: 115 6043 54 1484 165 55
Fragments arranged by size:
6043 1484 165 115 55 54
BsrDI GCAATG_nn'
Cuts at: 689 841 4146 4304 5828 689
Size: 152 3305 158 1524 2777
Fragments arranged by size:
3305 2777 1524 158 152
BsrFI r'CCGG_y
Cuts at: 2467 5914 6095 2467
Size: 3447 181 4288
Fragments arranged by size:
4288 3447 181
BssSI C'ACGA_G
Cuts at: 4780 6187 6895 4780
Size: 1407 708 5801
Fragments arranged by size:
5801 1407 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1170 4830 5293 1170
Size: 3660 463 3793
Fragments arranged by size:
3793 3660 463
BstBI TT'CG_AA
Cuts at: 3261 6277 3261
Size: 3016 4900
BstEII G'GTnAC_C
Cuts at: 1579 1579
Size: 7916
BstXI CCAn_nnnn'nTGG
Cuts at: 2325 2697 4259 2325
Size: 372 1562 5982
Fragments arranged by size:
5982 1562 372
BstYI r'GATC_y
Cuts at: 1 346 837 855 1413 3051 4941 5408 5431
Size: 345 491 18 558 1638 1890 467 23
Cuts at: 5431 5766 6012 6318 6330 6416 7468 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1890 1638 1052 558 491 467 449 345
335 306 246 86 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 2513 2513
Size: 7916
BtgI C'CryG_G
Cuts at: 119 368 119
Size: 249 7667
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5866 6047 6210 5866
Size: 181 163 7572
Fragments arranged by size:
7572 181 163
BtsI GCAGTG_nn'
Cuts at: 4381 4718 5159 4381
Size: 337 441 7138
Fragments arranged by size:
7138 441 337
ClaI AT'CG_AT
Cuts at: 2762 2762
Size: 7916
DraI TTT'AAA
Cuts at: 1073 1247 4915 1073
Size: 174 3668 4074
Fragments arranged by size:
4074 3668 174
DraIII CAC_nnn'GTG
Cuts at: 1353 1353
Size: 7916
DrdI GACnn_nn'nnGTC
Cuts at: 663 5622 6966 663
Size: 4959 1344 1613
Fragments arranged by size:
4959 1613 1344
EaeI y'GGCC_r
Cuts at: 2699 3600 5501 5675 6066 6093 7438 2699
Size: 901 1901 174 391 27 1345 3177
Fragments arranged by size:
3177 1901 1345 901 391 174 27
EagI C'GGCC_G
Cuts at: 5501 5501
Size: 7916
EarI CTCTTCn'nnn_
Cuts at: 608 803 815 1277 2803 3148 4139 5939 6149
Size: 195 12 462 1526 345 991 1800 210
Cuts at: 6149 608
Size: 2375
Fragments arranged by size:
2375 1800 1526 991 462 345 210 195
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3238 6124 6866 7012 7726 3238
Size: 2886 742 146 714 3428
Fragments arranged by size:
3428 2886 742 714 146
EcoICRI GAG'CTC
Cuts at: 4928 4928
Size: 7916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 943 1040 2327 2888 3563 3637 3773 3882
Size: 515 97 1287 561 675 74 136 109
Cuts at: 3882 4031 4331 4398 4517 4616 4679 5741 6173
Size: 149 300 67 119 99 63 1062 432
Cuts at: 6173 6526 7188 428
Size: 353 662 1156
Fragments arranged by size:
1287 1156 1062 675 662 561 515 432
353 300 149 136 119 109 99 97
74 67 63
EcoNI CCTnn'n_nnAGG
Cuts at: 3848 4850 3848
Size: 1002 6914
EcoO109I rG'GnC_Cy
Cuts at: 784 3996 4672 5055 784
Size: 3212 676 383 3645
Fragments arranged by size:
3645 3212 676 383
EcoRI G'AATT_C
Cuts at: 2570 4920 2570
Size: 2350 5566
EcoRV GAT'ATC
Cuts at: 726 2349 726
Size: 1623 6293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 4220 5935 1978
Size: 2242 1715 3959
Fragments arranged by size:
3959 2242 1715
FspI TGC'GCA
Cuts at: 5697 5697
Size: 7916
HaeII r_GCGC'y
Cuts at: 134 561 5350 5598 6828 7599 134
Size: 427 4789 248 1230 771 451
Fragments arranged by size:
4789 1230 771 451 427 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1629 1661 2150 2182 2536 2568 3538 3570 3874
Size: 32 489 32 354 32 970 32 304
Cuts at: 3874 3906 3943 3975 4044 4076 4794 4826 5758
Size: 32 37 32 69 32 718 32 932
Cuts at: 5758 5788 5790 5820 1629
Size: 30 2 30 3725
Fragments arranged by size:
3725 970 932 718 489 354 304 69
37 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 588 3951 4069 4955 588
Size: 3363 118 886 3549
Fragments arranged by size:
3549 3363 886 118
HpaI GTT'AAC
Cuts at: 588 588
Size: 7916
KasI G'GCGC_C
Cuts at: 5346 5594 5346
Size: 248 7668
KpnI G_GTAC'C
Cuts at: 3527 4936 3527
Size: 1409 6507
MfeI C'AATT_G
Cuts at: 916 3939 916
Size: 3023 4893
MluI A'CGCG_T
Cuts at: 7349 7349
Size: 7916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2306 2501 4180 4384 6675 6859 1441
Size: 865 195 1679 204 2291 184 2498
Fragments arranged by size:
2498 2291 1679 865 204 195 184
MscI TGG'CCA
Cuts at: 2701 3602 5677 2701
Size: 901 2075 4940
Fragments arranged by size:
4940 2075 901
MslI CAynn'nnrTG
Cuts at: 961 1100 1565 1945 2320 2695 3504 4295 5905
Size: 139 465 380 375 375 809 791 1610
Cuts at: 5905 6032 961
Size: 127 2845
Fragments arranged by size:
2845 1610 809 791 465 380 375 375
139 127
MspA1I CmG'CkG
Cuts at: 610 2709 3598 3664 4180 4486 5027 5341 5701
Size: 2099 889 66 516 306 541 314 360
Cuts at: 5701 6485 6730 610
Size: 784 245 1796
Fragments arranged by size:
2099 1796 889 784 541 516 360 314
306 245 66
NaeI GCC'GGC
Cuts at: 6097 6097
Size: 7916
NarI GG'CG_CC
Cuts at: 5347 5595 5347
Size: 248 7668
NcoI C'CATG_G
Cuts at: 368 368
Size: 7916
NdeI CA'TA_TG
Cuts at: 3519 3519
Size: 7916
NgoMIV G'CCGG_C
Cuts at: 6095 6095
Size: 7916
NsiI A_TGCA'T
Cuts at: 629 1175 1388 4736 629
Size: 546 213 3348 3809
Fragments arranged by size:
3809 3348 546 213
NspI r_CATG'y
Cuts at: 1173 2050 3342 4969 5469 7072 1173
Size: 877 1292 1627 500 1603 2017
Fragments arranged by size:
2017 1627 1603 1292 877 500
PasI CC'CwG_GG
Cuts at: 4078 4078
Size: 7916
PciI A'CATG_T
Cuts at: 7068 7068
Size: 7916
PflMI CCAn_nnn'nTGG
Cuts at: 326 4447 326
Size: 4121 3795
PfoI T'CCnGG_A
Cuts at: 4632 4632
Size: 7916
PmeI GTTT'AAAC
Cuts at: 4915 4915
Size: 7916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 845 877 3469 3501 3927 3959 6362 6394 845
Size: 32 2592 32 426 32 2403 32 2367
Fragments arranged by size:
2592 2403 2367 426 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 784 4672 784
Size: 3888 4028
PshAI GACnn'nnGTC
Cuts at: 3306 4187 3306
Size: 881 7035
PsiI TTA'TAA
Cuts at: 2365 4535 2365
Size: 2170 5746
PstI C_TGCA'G
Cuts at: 891 2309 2681 3966 4963 5648 891
Size: 1418 372 1285 997 685 3159
Fragments arranged by size:
3159 1418 1285 997 685 372
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7916
PvuII CAG'CTG
Cuts at: 610 2709 3598 3664 4180 4486 5341 5701 610
Size: 2099 889 66 516 306 855 360 2825
Fragments arranged by size:
2825 2099 889 855 516 360 306 66
RsrII CG'GwC_CG
Cuts at: 6111 6111
Size: 7916
SacI G_AGCT'C
Cuts at: 4930 4930
Size: 7916
SalI G'TCGA_C
Cuts at: 4953 4953
Size: 7916
SapI GCTCTTCn'nnn_
Cuts at: 5939 6149 5939
Size: 210 7706
SbfI CC_TGCA'GG
Cuts at: 4963 4963
Size: 7916
ScaI AGT'ACT
Cuts at: 7433 7433
Size: 7916
SexAI A'CCwGG_T
Cuts at: 1794 1794
Size: 7916
SfcI C'TryA_G
Cuts at: 33 887 2241 2305 2677 2990 3962 3970 4959
Size: 854 1354 64 372 313 972 8 989
Cuts at: 4959 5644 6612 6803 33
Size: 685 968 191 1146
Fragments arranged by size:
1354 1146 989 972 968 854 685 372
313 191 64 8
SfoI GGC'GCC
Cuts at: 5348 5596 5348
Size: 248 7668
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7916
SmaI CCC'GGG
Cuts at: 4938 4938
Size: 7916
SmlI C'TyrA_G
Cuts at: 1515 2642 3833 4154 5071 5986 6700 6962 1515
Size: 1127 1191 321 917 915 714 262 2469
Fragments arranged by size:
2469 1191 1127 917 915 714 321 262
SpeI A'CTAG_T
Cuts at: 67 2649 67
Size: 2582 5334
SphI G_CATG'C
Cuts at: 4969 5469 4969
Size: 500 7416
SspI AAT'ATT
Cuts at: 218 1665 1872 2742 3102 218
Size: 1447 207 870 360 5032
Fragments arranged by size:
5032 1447 870 360 207
StuI AGG'CCT
Cuts at: 2198 2198
Size: 7916
StyI C'CwwG_G
Cuts at: 368 3239 4511 5050 5404 368
Size: 2871 1272 539 354 2880
Fragments arranged by size:
2880 2871 1272 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2719 4070 4605 5499 5632 6091 6300 2719
Size: 1351 535 894 133 459 209 4335
Fragments arranged by size:
4335 1351 894 535 459 209 133
TatI w'GTAC_w
Cuts at: 1166 2756 4552 4574 7431 1166
Size: 1590 1796 22 2857 1651
Fragments arranged by size:
2857 1796 1651 1590 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1337 1369 3849 3881 3967 3999 7433 7465 1337
Size: 32 2480 32 86 32 3434 32 1788
Fragments arranged by size:
3434 2480 1788 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 963 5713 963
Size: 4750 3166
XbaI T'CTAG_A
Cuts at: 1602 3076 4360 4747 4947 1602
Size: 1474 1284 387 200 4571
Fragments arranged by size:
4571 1474 1284 387 200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2322 4023 5411 2322
Size: 1701 1388 4827
Fragments arranged by size:
4827 1701 1388
XmaI C'CCGG_G
Cuts at: 4936 4936
Size: 7916
XmnI GAAnn'nnTTC
Cuts at: 1433 3234 3267 1433
Size: 1801 33 6082
Fragments arranged by size:
6082 1801 33
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI ApaI AscI AvrII BaeI
BbvCI BcgI BmtI BsiWI BspEI BsrGI BssHII BstZ17I
CspCI FseI FspAI HindIII NheI NotI NruI PacI
PmlI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI