Restriction map for
pF1KB3465
(Circular) (Six-base) MAPSORT of: pF1KB3465.seq Check: 8871 from: 1 to: 4148
pF1KB3465 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 764 1973 2405 2758 3420 373
Size: 391 1209 432 353 662 1101
Fragments arranged by size:
1209 1101 662 432 391 353
AflIII A'CryG_T
Cuts at: 1500 3300 3581 1500
Size: 1800 281 2067
Fragments arranged by size:
2067 1800 281
AleI CACnn'nnGTG
Cuts at: 890 890
Size: 4148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 760 792 760
Size: 32 4116
AlwNI CAG_nnn'CTG
Cuts at: 130 1453 2891 130
Size: 1323 1438 1387
Fragments arranged by size:
1438 1387 1323
ApaLI G'TGCA_C
Cuts at: 996 2986 996
Size: 1990 2158
ApoI r'AATT_y
Cuts at: 336 630 1152 3501 3757 336
Size: 294 522 2349 256 727
Fragments arranged by size:
2349 727 522 294 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 522 1168 522
Size: 646 3502
BamHI G'GATC_C
Cuts at: 527 1173 527
Size: 646 3502
BanI G'GyrC_C
Cuts at: 1164 1578 1826 1861 1164
Size: 414 248 35 3451
Fragments arranged by size:
3451 414 248 35
BanII G_rGCy'C
Cuts at: 523 1162 2192 523
Size: 639 1030 2479
Fragments arranged by size:
2479 1030 639
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4114
BciVI GTATCCnnnnn_n'
Cuts at: 855 875 2046 3102 4148 855
Size: 20 1171 1056 1046 855
Fragments arranged by size:
1171 1056 1046 855 20
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 1000 1075 1773 1866 2990 1000
Size: 75 698 93 1124 2158
Fragments arranged by size:
2158 1124 698 93 75
BmrI ACTGGGnnnn_n'
Cuts at: 118 1360 1441 1776 3920 118
Size: 1242 81 335 2144 346
Fragments arranged by size:
2144 1242 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 574 425
Size: 149 3999
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2917 3215 1324
Size: 879 714 298 2257
Fragments arranged by size:
2257 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 612 831 3852 612
Size: 219 3021 908
Fragments arranged by size:
3021 908 219
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 737 1662 3699 6
Size: 731 925 2037 455
Fragments arranged by size:
2037 925 731 455
BsaHI Gr'CG_yC
Cuts at: 1579 1827 2529 4025 1579
Size: 248 702 1496 1702
Fragments arranged by size:
1702 1496 702 248
BsaWI w'CCGG_w
Cuts at: 371 1337 1858 2536 2947 3094 3368 371
Size: 966 521 678 411 147 274 1151
Fragments arranged by size:
1151 966 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 336 634 336
Size: 298 3850
BseYI C'CCAG_C
Cuts at: 331 1573 2331 2996 3404 331
Size: 1242 758 665 408 1075
Fragments arranged by size:
1242 1075 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 577 844 469
Size: 108 267 3773
Fragments arranged by size:
3773 267 108
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2966 104
Size: 1586 46 1230 1286
Fragments arranged by size:
1586 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1000 1162 1940 2130 2990 1000
Size: 162 778 190 860 2158
Fragments arranged by size:
2158 860 778 190 162
Bsp1286I G_dGCh'C
Cuts at: 523 1000 1075 1162 1773 1866 1940 2130 2192
Size: 477 75 87 611 93 74 190 62
Cuts at: 2192 2990 523
Size: 798 1681
Fragments arranged by size:
1681 798 611 477 190 93 87 75
74 62
BspHI T'CATG_A
Cuts at: 2580 2580
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1198 1412 1714 2095 1198
Size: 214 302 381 3251
Fragments arranged by size:
3251 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 229 2060 229
Size: 1831 2317
BsrFI r'CCGG_y
Cuts at: 546 2146 2327 546
Size: 1600 181 2367
Fragments arranged by size:
2367 1600 181
BsrGI T'GTAC_A
Cuts at: 575 917 982 575
Size: 342 65 3741
Fragments arranged by size:
3741 342 65
BssSI C'ACGA_G
Cuts at: 2419 3127 2419
Size: 708 3440
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstYI r'GATC_y
Cuts at: 1 527 1173 1640 1663 1998 2244 2550 2562
Size: 526 646 467 23 335 246 306 12
Cuts at: 2562 2648 3700 1
Size: 86 1052 449
Fragments arranged by size:
1052 646 526 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 146 146
Size: 4148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 1854 3198 1854
Size: 1344 2804
EaeI y'GGCC_r
Cuts at: 1733 1907 2298 2325 3670 1733
Size: 174 391 27 1345 2211
Fragments arranged by size:
2211 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 829 2171 2381 829
Size: 1342 210 2596
Fragments arranged by size:
2596 1342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 3098 3244 3958 2356
Size: 742 146 714 2546
Fragments arranged by size:
2546 742 714 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 425 574 764 1973 2405 2758 3420 373
Size: 52 149 190 1209 432 353 662 1101
Fragments arranged by size:
1209 1101 662 432 353 190 149 52
EcoNI CCTnn'n_nnAGG
Cuts at: 440 440
Size: 4148
EcoO109I rG'GnC_Cy
Cuts at: 1287 1287
Size: 4148
EcoRI G'AATT_C
Cuts at: 336 1152 336
Size: 816 3332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 1582 1830 3060 3831 1582
Size: 248 1230 771 1899
Fragments arranged by size:
1899 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1990 2020 2022 2052 142
Size: 32 1816 30 2 30 2238
Fragments arranged by size:
2238 1816 32 30 30 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2907 3091 2907
Size: 184 3964
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 112 890 2137 2264 112
Size: 778 1247 127 1996
Fragments arranged by size:
1996 1247 778 127
MspA1I CmG'CkG
Cuts at: 468 493 1259 1573 1933 2717 2962 468
Size: 25 766 314 360 784 245 1654
Fragments arranged by size:
1654 784 766 360 314 245 25
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NcoI C'CATG_G
Cuts at: 146 146
Size: 4148
NdeI CA'TA_TG
Cuts at: 669 669
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NspI r_CATG'y
Cuts at: 399 648 1137 1201 1701 3304 399
Size: 249 489 64 500 1603 1243
Fragments arranged by size:
1603 1243 500 489 249 64
PciI A'CATG_T
Cuts at: 3300 3300
Size: 4148
PflMI CCAn_nnn'nTGG
Cuts at: 549 549
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2594 2626 2594
Size: 32 4116
PstI C_TGCA'G
Cuts at: 1195 1880 1195
Size: 685 3463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 493 1573 1933 493
Size: 1080 360 2708
Fragments arranged by size:
2708 1080 360
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 1191 1876 2844 3035 33
Size: 1158 685 968 191 1146
Fragments arranged by size:
1158 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 524 1170 524
Size: 646 3502
SmlI C'TyrA_G
Cuts at: 1303 2218 2932 3194 1303
Size: 915 714 262 2257
Fragments arranged by size:
2257 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 399 648 1201 1701 399
Size: 249 553 500 2846
Fragments arranged by size:
2846 553 500 249
StyI C'CwwG_G
Cuts at: 146 1282 1636 146
Size: 1136 354 2658
Fragments arranged by size:
2658 1136 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 804 1004 1731 1864 2323 2532 804
Size: 200 727 133 459 209 2420
Fragments arranged by size:
2420 727 459 209 200 133
TatI w'GTAC_w
Cuts at: 575 917 982 3663 575
Size: 342 65 2681 1060
Fragments arranged by size:
2681 1060 342 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 4116
Tth111I GACn'n_nGTC
Cuts at: 154 1945 154
Size: 1791 2357
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 522 1168 522
Size: 646 3502
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI