Restriction map for
pF1KB3466
(Circular) (Six-base) MAPSORT of: pF1KB3466.seq Check: 6800 from: 1 to: 4844
pF1KB3466 4844 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1882 1882
Size: 4844
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 4684 4684
Size: 4844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 708 1180 1316 1516 1693 2669 3101 3454
Size: 5 472 136 200 177 976 432 353
Cuts at: 3454 4116 703
Size: 662 1431
Fragments arranged by size:
1431 976 662 472 432 353 200 177
136 5
AflIII A'CryG_T
Cuts at: 2196 3996 4277 2196
Size: 1800 281 2763
Fragments arranged by size:
2763 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 220 252 220
Size: 32 4812
AlwNI CAG_nnn'CTG
Cuts at: 408 1531 2149 3587 408
Size: 1123 618 1438 1665
Fragments arranged by size:
1665 1438 1123 618
ApaLI G'TGCA_C
Cuts at: 3682 3682
Size: 4844
ApoI r'AATT_y
Cuts at: 868 1825 1848 4197 4453 868
Size: 957 23 2349 256 1259
Fragments arranged by size:
2349 1259 957 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 1034 1328 1864 1034
Size: 294 536 4014
Fragments arranged by size:
4014 536 294
BamHI G'GATC_C
Cuts at: 620 1869 620
Size: 1249 3595
BanI G'GyrC_C
Cuts at: 1860 2274 2522 2557 1860
Size: 414 248 35 4147
Fragments arranged by size:
4147 414 248 35
BanII G_rGCy'C
Cuts at: 1224 1858 2888 1224
Size: 634 1030 3180
Fragments arranged by size:
3180 1030 634
BbeI G_GCGC'C
Cuts at: 2278 2526 2278
Size: 248 4596
BbsI GAAGACnn'nnnn_
Cuts at: 903 1475 1486 1504 1726 903
Size: 572 11 18 222 4021
Fragments arranged by size:
4021 572 222 18 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 108 124 142 90
Size: 18 16 18 4792
Fragments arranged by size:
4792 18 18 16
BciVI GTATCCnnnnn_n'
Cuts at: 2742 3798 4844 2742
Size: 1056 1046 2742
Fragments arranged by size:
2742 1056 1046
BclI T'GATC_A
Cuts at: 2364 2364
Size: 4844
BglI GCCn_nnn'nGGC
Cuts at: 1309 1309
Size: 4844
BglII A'GATC_T
Cuts at: 696 2359 4396 696
Size: 1663 2037 1144
Fragments arranged by size:
2037 1663 1144
BlpI GC'TnA_GC
Cuts at: 1190 1490 1956 1190
Size: 300 466 4078
Fragments arranged by size:
4078 466 300
Bme1580I G_kGCm'C
Cuts at: 1254 2469 2562 3686 1254
Size: 1215 93 1124 2412
Fragments arranged by size:
2412 1215 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1768 2056 2137 2472 4616 1768
Size: 288 81 335 2144 1996
Fragments arranged by size:
2144 1996 335 288 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1532 3211 1532
Size: 1679 3165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 721 852 1420 175
Size: 546 131 568 3599
Fragments arranged by size:
3599 568 546 131
Bpu10I CC'TnA_GC
Cuts at: 655 655
Size: 4844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 677 1252 2020 2899 3613 3911 243
Size: 434 575 768 879 714 298 1176
Fragments arranged by size:
1176 879 768 714 575 434 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 784 4548 460
Size: 324 3764 756
Fragments arranged by size:
3764 756 324
BsaAI yAC'GTr
Cuts at: 2197 2197
Size: 4844
BsaBI GATnn'nnATC
Cuts at: 6 2358 4395 6
Size: 2352 2037 455
Fragments arranged by size:
2352 2037 455
BsaHI Gr'CG_yC
Cuts at: 2275 2523 3225 4721 2275
Size: 248 702 1496 2398
Fragments arranged by size:
2398 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2033 2554 3232 3643 3790 4064 2033
Size: 521 678 411 147 274 2813
Fragments arranged by size:
2813 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 628 1054 1156 1636 628
Size: 426 102 480 3836
Fragments arranged by size:
3836 480 426 102
BseYI C'CCAG_C
Cuts at: 759 1394 2269 3027 3692 4100 759
Size: 635 875 758 665 408 1503
Fragments arranged by size:
1503 875 758 665 635 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1681 1033
Size: 648 4196
BsiEI CG_ry'CG
Cuts at: 104 2386 2432 3662 104
Size: 2282 46 1230 1286
Fragments arranged by size:
2282 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1858 2636 2826 3686 1858
Size: 778 190 860 3016
Fragments arranged by size:
3016 860 778 190
BsmI GAATG_Cn'
Cuts at: 434 434
Size: 4844
Bsp1286I G_dGCh'C
Cuts at: 1224 1254 1858 2469 2562 2636 2826 2888 3686
Size: 30 604 611 93 74 190 62 798
Cuts at: 3686 1224
Size: 2382
Fragments arranged by size:
2382 798 611 604 190 93 74 62
30
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1894 2108 2410 2791 1894
Size: 214 302 381 3947
Fragments arranged by size:
3947 381 302 214
BsrBI CCG'CTC
Cuts at: 50 618 3136 3190 4674 4839 50
Size: 568 2518 54 1484 165 55
Fragments arranged by size:
2518 1484 568 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2756 2756
Size: 4844
BsrFI r'CCGG_y
Cuts at: 2842 3023 2842
Size: 181 4663
BssSI C'ACGA_G
Cuts at: 3115 3823 3115
Size: 708 4136
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 2221 1531
Size: 690 4154
BstBI TT'CG_AA
Cuts at: 3205 3205
Size: 4844
BstYI r'GATC_y
Cuts at: 1 620 696 944 1790 1869 2336 2359 2694
Size: 619 76 248 846 79 467 23 335
Cuts at: 2694 2940 3246 3258 3344 4396 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 846 619 467 449 335 306 248
246 86 79 76 23 12
Bsu36I CC'TnA_GG
Cuts at: 365 365
Size: 4844
BtgI C'CryG_G
Cuts at: 140 645 140
Size: 505 4339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2794 2975 3138 2794
Size: 181 163 4500
Fragments arranged by size:
4500 181 163
BtsI GCAGTG_nn'
Cuts at: 1623 2087 1623
Size: 464 4380
DraI TTT'AAA
Cuts at: 1843 1843
Size: 4844
DrdI GACnn_nn'nnGTC
Cuts at: 2550 3894 2550
Size: 1344 3500
EaeI y'GGCC_r
Cuts at: 548 1274 1604 1694 2429 2603 2994 3021 4366
Size: 726 330 90 735 174 391 27 1345
Cuts at: 4366 548
Size: 1026
Fragments arranged by size:
1345 1026 735 726 391 330 174 90
27
EagI C'GGCC_G
Cuts at: 2429 2429
Size: 4844
EarI CTCTTCn'nnn_
Cuts at: 1292 2867 3077 1292
Size: 1575 210 3059
Fragments arranged by size:
3059 1575 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3052 3794 3940 4654 3052
Size: 742 146 714 3242
Fragments arranged by size:
3242 742 714 146
EcoICRI GAG'CTC
Cuts at: 1856 1856
Size: 4844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 703 708 721 852 1180 1316 1420 1516
Size: 528 5 13 131 328 136 104 96
Cuts at: 1516 1693 2669 3101 3454 4116 175
Size: 177 976 432 353 662 903
Fragments arranged by size:
976 903 662 528 432 353 328 177
136 131 104 96 13 5
EcoO109I rG'GnC_Cy
Cuts at: 1677 1983 1677
Size: 306 4538
EcoRI G'AATT_C
Cuts at: 1848 1848
Size: 4844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 840 963 1329 2863 840
Size: 123 366 1534 2821
Fragments arranged by size:
2821 1534 366 123
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 408 2278 2526 3756 4527 408
Size: 1870 248 1230 771 725
Fragments arranged by size:
1870 1230 771 725 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 2686 2716 2718 2748 358
Size: 32 2296 30 2 30 2454
Fragments arranged by size:
2454 2296 32 30 30 2
HincII GTy'rAC
Cuts at: 1883 1883
Size: 4844
KasI G'GCGC_C
Cuts at: 2274 2522 2274
Size: 248 4596
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MluI A'CGCG_T
Cuts at: 4277 4277
Size: 4844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3603 3787 3603
Size: 184 4660
MscI TGG'CCA
Cuts at: 1276 1606 1696 2605 1276
Size: 330 90 909 3515
Fragments arranged by size:
3515 909 330 90
MslI CAynn'nnrTG
Cuts at: 1009 2833 2960 1009
Size: 1824 127 2893
Fragments arranged by size:
2893 1824 127
MspA1I CmG'CkG
Cuts at: 472 1356 1398 1955 2269 2629 3413 3658 472
Size: 884 42 557 314 360 784 245 1658
Fragments arranged by size:
1658 884 784 557 360 314 245 42
NaeI GCC'GGC
Cuts at: 3025 3025
Size: 4844
NarI GG'CG_CC
Cuts at: 2275 2523 2275
Size: 248 4596
NcoI C'CATG_G
Cuts at: 140 140
Size: 4844
NgoMIV G'CCGG_C
Cuts at: 3023 3023
Size: 4844
NspI r_CATG'y
Cuts at: 654 984 1755 1897 2397 4000 654
Size: 330 771 142 500 1603 1498
Fragments arranged by size:
1603 1498 771 500 330 142
PasI CC'CwG_GG
Cuts at: 774 774
Size: 4844
PciI A'CATG_T
Cuts at: 3996 3996
Size: 4844
PfoI T'CCnGG_A
Cuts at: 578 671 578
Size: 93 4751
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4812
PstI C_TGCA'G
Cuts at: 251 1322 1891 2576 251
Size: 1071 569 685 2519
Fragments arranged by size:
2519 1071 685 569
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 1356 1398 2269 2629 1356
Size: 42 871 360 3571
Fragments arranged by size:
3571 871 360 42
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 1858 1858
Size: 4844
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 2867 3077 2867
Size: 210 4634
SbfI CC_TGCA'GG
Cuts at: 1891 1891
Size: 4844
ScaI AGT'ACT
Cuts at: 4361 4361
Size: 4844
SexAI A'CCwGG_T
Cuts at: 200 200
Size: 4844
SfcI C'TryA_G
Cuts at: 33 247 1318 1887 2572 3540 3731 33
Size: 214 1071 569 685 968 191 1146
Fragments arranged by size:
1146 1071 968 685 569 214 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1309 1309
Size: 4844
SfoI GGC'GCC
Cuts at: 2276 2524 2276
Size: 248 4596
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 1036 1330 1866 1036
Size: 294 536 4014
Fragments arranged by size:
4014 536 294
SmlI C'TyrA_G
Cuts at: 222 692 1231 1999 2914 3628 3890 222
Size: 470 539 768 915 714 262 1176
Fragments arranged by size:
1176 915 768 714 539 470 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4844
SphI G_CATG'C
Cuts at: 654 1897 2397 654
Size: 1243 500 3101
Fragments arranged by size:
3101 1243 500
StuI AGG'CCT
Cuts at: 1312 1324 1312
Size: 12 4832
StyI C'CwwG_G
Cuts at: 140 1277 1304 1361 1607 1978 2332 140
Size: 1137 27 57 246 371 354 2652
Fragments arranged by size:
2652 1137 371 354 246 57 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2427 2560 3019 3228 2427
Size: 133 459 209 4043
Fragments arranged by size:
4043 459 209 133
TatI w'GTAC_w
Cuts at: 1510 4359 1510
Size: 2849 1995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4361 4393 4361
Size: 32 4812
Tth111I GACn'n_nGTC
Cuts at: 907 2641 907
Size: 1734 3110
XbaI T'CTAG_A
Cuts at: 1875 1875
Size: 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 505 2339 505
Size: 1834 3010
XmaI C'CCGG_G
Cuts at: 1034 1328 1864 1034
Size: 294 536 4014
Fragments arranged by size:
4014 536 294
XmnI GAAnn'nnTTC
Cuts at: 386 721 386
Size: 335 4509
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI