Restriction map for pF1KB3471
 (Circular) (Six-base) MAPSORT of: pF1KB3471.seq  Check: 2516  from: 1  to: 4715



pF1KB3471  4715 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    863    1753     863 
   Size:        890    3825
 
Acc65I G'GTAC_C

Cuts at:   1731    1731 
   Size:       4715
 
AclI AA'CG_TT

Cuts at:   4555    4555 
   Size:       4715
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    748    1194    1249    2540    2972    3325    3987     748 
   Size:        446      55    1291     432     353     662    1476

  Fragments arranged by size: 

                1476    1291     662     446     432     353      55
 
AflIII A'CryG_T

Cuts at:    485    2067    3867    4148     485 
   Size:       1582    1800     281    1052

  Fragments arranged by size: 

                1800    1582    1052     281
 
AhdI GACnn_n'nnGTC

Cuts at:    954     954 
   Size:       4715
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    682     682 
   Size:       4715
 
AlwNI CAG_nnn'CTG

Cuts at:    204     792     960    1227    2020    3458     204 
   Size:        588     168     267     793    1438    1461

  Fragments arranged by size: 

                1461    1438     793     588     267     168
 
ApaI G_GGCC'C

Cuts at:    377     813     377 
   Size:        436    4279
 
ApaLI G'TGCA_C

Cuts at:   3553    3553 
   Size:       4715
 
ApoI r'AATT_y

Cuts at:   1719    4068    4324    1719 
   Size:       2349     256    2110

  Fragments arranged by size: 

                2349    2110     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4715
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4715
 
AvaI C'yCGr_G

Cuts at:    357     376     571     847    1167    1735     357 
   Size:         19     195     276     320     568    3337

  Fragments arranged by size: 

                3337     568     320     276     195      19
 
BamHI G'GATC_C

Cuts at:    575    1740     575 
   Size:       1165    3550
 
BanI G'GyrC_C

Cuts at:    432     464    1342    1354    1498    1683    1731    2145    2393 
   Size:         32     878      12     144     185      48     414     248

Cuts at:   2393    2428     432 
   Size:         35    2719

  Fragments arranged by size: 

                2719     878     414     248     185     144      48      35
                  32      12
 
BanII G_rGCy'C

Cuts at:    158     377     383     813    1729    2759     158 
   Size:        219       6     430     916    1030    2114

  Fragments arranged by size: 

                2114    1030     916     430     219       6
 
BbeI G_GCGC'C

Cuts at:    468    1346    1502    2149    2397     468 
   Size:        878     156     647     248    2786

  Fragments arranged by size: 

                2786     878     647     248     156
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    429     463     429 
   Size:         34    4681
 
BciVI GTATCCnnnnn_n'

Cuts at:   2613    3669    4715    2613 
   Size:       1056    1046    2613

  Fragments arranged by size: 

                2613    1056    1046
 
BclI T'GATC_A

Cuts at:    716     998    2235     716 
   Size:        282    1237    3196

  Fragments arranged by size: 

                3196    1237     282
 
BglI GCCn_nnn'nGGC

Cuts at:   1374    1374 
   Size:       4715
 
BglII A'GATC_T

Cuts at:   1118    1148    1457    1556    2230    4267    1118 
   Size:         30     309      99     674    2037    1566

  Fragments arranged by size: 

                2037    1566     674     309      99      30
 
BlpI GC'TnA_GC

Cuts at:   1827    1827 
   Size:       4715
 
Bme1580I G_kGCm'C

Cuts at:    377     525     813    1011    1379    2340    2433    3557     377 
   Size:        148     288     198     368     961      93    1124    1535

  Fragments arranged by size: 

                1535    1124     961     368     288     198     148      93
            
 
BmgBI CAC'GTC

Cuts at:   1416    1575    1416 
   Size:        159    4556
 
BmrI ACTGGGnnnn_n'

Cuts at:   1927    2008    2343    4487    1927 
   Size:         81     335    2144    2155

  Fragments arranged by size: 

                2155    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    261    3082     261 
   Size:       2821    1894
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    235     277     605     652    1663     235 
   Size:         42     328      47    1011    3287

  Fragments arranged by size: 

                3287    1011     328      47      42
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1033    1891    2770    3484    3782    1033 
   Size:        858     879     714     298    1966

  Fragments arranged by size: 

                1966     879     858     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4419    4419 
   Size:       4715
 
BsaAI yAC'GTr

Cuts at:   2068    2068 
   Size:       4715
 
BsaBI GATnn'nnATC

Cuts at:      6     367    2229    4266       6 
   Size:        361    1862    2037     455

  Fragments arranged by size: 

                2037    1862     455     361
 
BsaHI Gr'CG_yC

Cuts at:    465     677    1343    1499    2146    2394    3096    4592     465 
   Size:        212     666     156     647     248     702    1496     588

  Fragments arranged by size: 

                1496     702     666     647     588     248     212     156
            
 
BsaWI w'CCGG_w

Cuts at:   1904    2425    3103    3514    3661    3935    1904 
   Size:        521     678     411     147     274    2684

  Fragments arranged by size: 

                2684     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    856     886    1135    1165     856 
   Size:         30     249      30    4406

  Fragments arranged by size: 

                4406     249      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    232     283     883     943    1321    1618     232 
   Size:         51     600      60     378     297    3329

  Fragments arranged by size: 

                3329     600     378     297      60      51
 
BseYI C'CCAG_C

Cuts at:    116     524    2140    2898    3563    3971     116 
   Size:        408    1616     758     665     408     860

  Fragments arranged by size: 

                1616     860     758     665     408     408
 
BsiEI CG_ry'CG

Cuts at:    104    2257    2303    3533     104 
   Size:       2153      46    1230    1286

  Fragments arranged by size: 

                2153    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    158     224     293    1145    1729    2507    2697    3557     158 
   Size:         66      69     852     584     778     190     860    1316

  Fragments arranged by size: 

                1316     860     852     778     584     190      69      66
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    775     775 
   Size:       4715
 
Bsp1286I G_dGCh'C

Cuts at:    158     224     293     377     383     525     813    1011    1145 
   Size:         66      69      84       6     142     288     198     134

Cuts at:   1145    1379    1729    2340    2433    2507    2697    2759    3557 
   Size:        234     350     611      93      74     190      62     798

Cuts at:   3557     158 
   Size:       1316

  Fragments arranged by size: 

                1316     798     611     350     288     234     198     190
                 142     134      93      84      74      69      66      62
                   6
 
BspHI T'CATG_A

Cuts at:   3147    3147 
   Size:       4715
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1676    1765    1979    2281    2662    1676 
   Size:         89     214     302     381    3729

  Fragments arranged by size: 

                3729     381     302     214      89
 
BsrBI CCG'CTC

Cuts at:     50     891    3007    3061    4545    4710      50 
   Size:        841    2116      54    1484     165      55

  Fragments arranged by size: 

                2116    1484     841     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2627    2627 
   Size:       4715
 
BsrFI r'CCGG_y

Cuts at:   1396    2713    2894    1396 
   Size:       1317     181    3217

  Fragments arranged by size: 

                3217    1317     181
 
BssSI C'ACGA_G

Cuts at:    235     295     448    1651    2986    3694     235 
   Size:         60     153    1203    1335     708    1256

  Fragments arranged by size: 

                1335    1256    1203     708     153      60
 
BstAPI GCAn_nnn'nTGC

Cuts at:    204     795    1683    2092     204 
   Size:        591     888     409    2827

  Fragments arranged by size: 

                2827     888     591     409
 
BstBI TT'CG_AA

Cuts at:   3076    3076 
   Size:       4715
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1242    1242 
   Size:       4715
 
BstYI r'GATC_y

Cuts at:      1     575     746    1118    1148    1457    1556    1740    2207 
   Size:        574     171     372      30     309      99     184     467

Cuts at:   2207    2230    2565    2811    3117    3129    3215    4267       1 
   Size:         23     335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052     574     467     449     372     335     309     306
                 246     184     171      99      86      30      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:    979     979 
   Size:       4715
 
BtgI C'CryG_G

Cuts at:    460     460 
   Size:       4715
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    384    1260    2665    2846    3009     384 
   Size:        876    1405     181     163    2090

  Fragments arranged by size: 

                2090    1405     876     181     163
 
BtsI GCAGTG_nn'

Cuts at:    958    1958     958 
   Size:       1000    3715
 
DraI TTT'AAA

Cuts at:   1714    1714 
   Size:       4715
 
DraIII CAC_nnn'GTG

Cuts at:   1683    1683 
   Size:       4715
 
DrdI GACnn_nn'nnGTC

Cuts at:   2421    3765    2421 
   Size:       1344    3371
 
EaeI y'GGCC_r

Cuts at:    170    1016    1265    1568    1616    2300    2474    2865    2892 
   Size:        846     249     303      48     684     174     391      27

Cuts at:   2892    4237     170 
   Size:       1345     648

  Fragments arranged by size: 

                1345     846     684     648     391     303     249     174
                  48      27
 
EagI C'GGCC_G

Cuts at:   2300    2300 
   Size:       4715
 
EarI CTCTTCn'nnn_

Cuts at:    163     676     946    2738    2948     163 
   Size:        513     270    1792     210    1930

  Fragments arranged by size: 

                1930    1792     513     270     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2923    3665    3811    4525    2923 
   Size:        742     146     714    3113

  Fragments arranged by size: 

                3113     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    156    1727     156 
   Size:       1571    3144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    235     277     605     652     748    1194    1249    1663    2540 
   Size:         42     328      47      96     446      55     414     877

Cuts at:   2540    2972    3325    3987     235 
   Size:        432     353     662     963

  Fragments arranged by size: 

                 963     877     662     446     432     414     353     328
                  96      55      47      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:    943    1630     943 
   Size:        687    4028
 
EcoO109I rG'GnC_Cy

Cuts at:    809     810     936    1854     809 
   Size:          1     126     918    3670

  Fragments arranged by size: 

                3670     918     126       1
 
EcoRI G'AATT_C

Cuts at:   1719    1719 
   Size:       4715
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    342    2734     342 
   Size:       2392    2323
 
FspI TGC'GCA

Cuts at:    367    2496     367 
   Size:       2129    2586
 
FspAI rTGC'GCAy

Cuts at:    367     367 
   Size:       4715
 
HaeII r_GCGC'y

Cuts at:    468    1228    1346    1502    2149    2397    3627    4398     468 
   Size:        760     118     156     647     248    1230     771     785

  Fragments arranged by size: 

                1230     785     771     760     647     248     156     118
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    326     358     691     708     723     740     940     972    2557 
   Size:         32     333      17      15      17     200      32    1585

Cuts at:   2557    2587    2589    2619     326 
   Size:         30       2      30    2422

  Fragments arranged by size: 

                2422    1585     333     200      32      32      30      30
                  17      17      15       2
 
HincII GTy'rAC

Cuts at:   1482    1754    1482 
   Size:        272    4443
 
KasI G'GCGC_C

Cuts at:    464    1342    1498    2145    2393     464 
   Size:        878     156     647     248    2786

  Fragments arranged by size: 

                2786     878     647     248     156
 
KpnI G_GTAC'C

Cuts at:   1735    1735 
   Size:       4715
 
MluI A'CGCG_T

Cuts at:    485    4148     485 
   Size:       3663    1052
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    357    3474    3658     357 
   Size:       3117     184    1414

  Fragments arranged by size: 

                3117    1414     184
 
MscI TGG'CCA

Cuts at:   1018    1267    1618    2476    1018 
   Size:        249     351     858    3257

  Fragments arranged by size: 

                3257     858     351     249
 
MslI CAynn'nnrTG

Cuts at:   2704    2831    2704 
   Size:        127    4588
 
MspA1I CmG'CkG

Cuts at:    116     189     462     528     597     792    1826    2140    2500 
   Size:         73     273      66      69     195    1034     314     360

Cuts at:   2500    3284    3529     116 
   Size:        784     245    1302

  Fragments arranged by size: 

                1302    1034     784     360     314     273     245     195
                  73      69      66
 
NaeI GCC'GGC

Cuts at:   1398    2896    1398 
   Size:       1498    3217
 
NarI GG'CG_CC

Cuts at:    465    1343    1499    2146    2394     465 
   Size:        878     156     647     248    2786

  Fragments arranged by size: 

                2786     878     647     248     156
 
NgoMIV G'CCGG_C

Cuts at:   1396    2894    1396 
   Size:       1498    3217
 
NspI r_CATG'y

Cuts at:   1263    1768    2268    3871    1263 
   Size:        505     500    1603    2107

  Fragments arranged by size: 

                2107    1603     505     500
 
PasI CC'CwG_GG

Cuts at:    825    1156     825 
   Size:        331    4384
 
PciI A'CATG_T

Cuts at:   3867    3867 
   Size:       4715
 
PflMI CCAn_nnn'nTGG

Cuts at:    168     687     168 
   Size:        519    4196
 
PfoI T'CCnGG_A

Cuts at:    542     629     542 
   Size:         87    4628
 
PmeI GTTT'AAAC

Cuts at:   1714    1714 
   Size:       4715
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3161    3193    3161 
   Size:         32    4683
 
PpuMI rG'GwC_Cy

Cuts at:    936     936 
   Size:       4715
 
PspOMI G'GGCC_C

Cuts at:    373     809     373 
   Size:        436    4279
 
PstI C_TGCA'G

Cuts at:   1199    1762    2447    1199 
   Size:        563     685    3467

  Fragments arranged by size: 

                3467     685     563
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4715
 
PvuII CAG'CTG

Cuts at:    116     189     528     792    2140    2500     116 
   Size:         73     339     264    1348     360    2331

  Fragments arranged by size: 

                2331    1348     360     339     264      73
 
RsrII CG'GwC_CG

Cuts at:   2910    2910 
   Size:       4715
 
SacI G_AGCT'C

Cuts at:    158    1729     158 
   Size:       1571    3144
 
SacII CC_GC'GG

Cuts at:    463     463 
   Size:       4715
 
SalI G'TCGA_C

Cuts at:   1752    1752 
   Size:       4715
 
SapI GCTCTTCn'nnn_

Cuts at:    163     676    2738    2948     163 
   Size:        513    2062     210    1930

  Fragments arranged by size: 

                2062    1930     513     210
 
SbfI CC_TGCA'GG

Cuts at:   1762    1762 
   Size:       4715
 
ScaI AGT'ACT

Cuts at:   4232    4232 
   Size:       4715
 
SexAI A'CCwGG_T

Cuts at:   1679    1679 
   Size:       4715
 
SfcI C'TryA_G

Cuts at:     33    1195    1758    2443    3411    3602      33 
   Size:       1162     563     685     968     191    1146

  Fragments arranged by size: 

                1162    1146     968     685     563     191
 
SfoI GGC'GCC

Cuts at:    466    1344    1500    2147    2395     466 
   Size:        878     156     647     248    2786

  Fragments arranged by size: 

                2786     878     647     248     156
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4715
 
SmaI CCC'GGG

Cuts at:    359     573    1737     359 
   Size:        214    1164    3337

  Fragments arranged by size: 

                3337    1164     214
 
SmlI C'TyrA_G

Cuts at:   1048    1870    2785    3499    3761    1048 
   Size:        822     915     714     262    2002

  Fragments arranged by size: 

                2002     915     822     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4715
 
SphI G_CATG'C

Cuts at:   1768    2268    1768 
   Size:        500    4215
 
StyI C'CwwG_G

Cuts at:   1019    1849    2203    1019 
   Size:        830     354    3531

  Fragments arranged by size: 

                3531     830     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1413    2298    2431    2890    3099    1413 
   Size:        885     133     459     209    3029

  Fragments arranged by size: 

                3029     885     459     209     133
 
TatI w'GTAC_w

Cuts at:    305    4230     305 
   Size:       3925     790
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1135    1167    4232    4264    1135 
   Size:         32    3065      32    1586

  Fragments arranged by size: 

                3065    1586      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2512    2512 
   Size:       4715
 
XbaI T'CTAG_A

Cuts at:   1746    1746 
   Size:       4715
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    426     540    1153    1515    2210     426 
   Size:        114     613     362     695    2931

  Fragments arranged by size: 

                2931     695     613     362     114
 
XmaI C'CCGG_G

Cuts at:    357     571    1735     357 
   Size:        214    1164    3337

  Fragments arranged by size: 

                3337    1164     214
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AleI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BmtI   Bpu10I     BsgI
    BsiWI     BsmI    BspEI    BsrGI   BssHII   BstEII  BstZ17I     ClaI
    CspCI    EcoRV     FseI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmlI    PshAI     PsiI
    PspXI     PsrI    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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