Restriction map for
pF1KB3471
(Circular) (Six-base) MAPSORT of: pF1KB3471.seq Check: 2516 from: 1 to: 4715
pF1KB3471 4715 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 863 1753 863
Size: 890 3825
Acc65I G'GTAC_C
Cuts at: 1731 1731
Size: 4715
AclI AA'CG_TT
Cuts at: 4555 4555
Size: 4715
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1194 1249 2540 2972 3325 3987 748
Size: 446 55 1291 432 353 662 1476
Fragments arranged by size:
1476 1291 662 446 432 353 55
AflIII A'CryG_T
Cuts at: 485 2067 3867 4148 485
Size: 1582 1800 281 1052
Fragments arranged by size:
1800 1582 1052 281
AhdI GACnn_n'nnGTC
Cuts at: 954 954
Size: 4715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 4715
AlwNI CAG_nnn'CTG
Cuts at: 204 792 960 1227 2020 3458 204
Size: 588 168 267 793 1438 1461
Fragments arranged by size:
1461 1438 793 588 267 168
ApaI G_GGCC'C
Cuts at: 377 813 377
Size: 436 4279
ApaLI G'TGCA_C
Cuts at: 3553 3553
Size: 4715
ApoI r'AATT_y
Cuts at: 1719 4068 4324 1719
Size: 2349 256 2110
Fragments arranged by size:
2349 2110 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4715
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
AvaI C'yCGr_G
Cuts at: 357 376 571 847 1167 1735 357
Size: 19 195 276 320 568 3337
Fragments arranged by size:
3337 568 320 276 195 19
BamHI G'GATC_C
Cuts at: 575 1740 575
Size: 1165 3550
BanI G'GyrC_C
Cuts at: 432 464 1342 1354 1498 1683 1731 2145 2393
Size: 32 878 12 144 185 48 414 248
Cuts at: 2393 2428 432
Size: 35 2719
Fragments arranged by size:
2719 878 414 248 185 144 48 35
32 12
BanII G_rGCy'C
Cuts at: 158 377 383 813 1729 2759 158
Size: 219 6 430 916 1030 2114
Fragments arranged by size:
2114 1030 916 430 219 6
BbeI G_GCGC'C
Cuts at: 468 1346 1502 2149 2397 468
Size: 878 156 647 248 2786
Fragments arranged by size:
2786 878 647 248 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 429 463 429
Size: 34 4681
BciVI GTATCCnnnnn_n'
Cuts at: 2613 3669 4715 2613
Size: 1056 1046 2613
Fragments arranged by size:
2613 1056 1046
BclI T'GATC_A
Cuts at: 716 998 2235 716
Size: 282 1237 3196
Fragments arranged by size:
3196 1237 282
BglI GCCn_nnn'nGGC
Cuts at: 1374 1374
Size: 4715
BglII A'GATC_T
Cuts at: 1118 1148 1457 1556 2230 4267 1118
Size: 30 309 99 674 2037 1566
Fragments arranged by size:
2037 1566 674 309 99 30
BlpI GC'TnA_GC
Cuts at: 1827 1827
Size: 4715
Bme1580I G_kGCm'C
Cuts at: 377 525 813 1011 1379 2340 2433 3557 377
Size: 148 288 198 368 961 93 1124 1535
Fragments arranged by size:
1535 1124 961 368 288 198 148 93
BmgBI CAC'GTC
Cuts at: 1416 1575 1416
Size: 159 4556
BmrI ACTGGGnnnn_n'
Cuts at: 1927 2008 2343 4487 1927
Size: 81 335 2144 2155
Fragments arranged by size:
2155 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 261 3082 261
Size: 2821 1894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 277 605 652 1663 235
Size: 42 328 47 1011 3287
Fragments arranged by size:
3287 1011 328 47 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1891 2770 3484 3782 1033
Size: 858 879 714 298 1966
Fragments arranged by size:
1966 879 858 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4419 4419
Size: 4715
BsaAI yAC'GTr
Cuts at: 2068 2068
Size: 4715
BsaBI GATnn'nnATC
Cuts at: 6 367 2229 4266 6
Size: 361 1862 2037 455
Fragments arranged by size:
2037 1862 455 361
BsaHI Gr'CG_yC
Cuts at: 465 677 1343 1499 2146 2394 3096 4592 465
Size: 212 666 156 647 248 702 1496 588
Fragments arranged by size:
1496 702 666 647 588 248 212 156
BsaWI w'CCGG_w
Cuts at: 1904 2425 3103 3514 3661 3935 1904
Size: 521 678 411 147 274 2684
Fragments arranged by size:
2684 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 1135 1165 856
Size: 30 249 30 4406
Fragments arranged by size:
4406 249 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 283 883 943 1321 1618 232
Size: 51 600 60 378 297 3329
Fragments arranged by size:
3329 600 378 297 60 51
BseYI C'CCAG_C
Cuts at: 116 524 2140 2898 3563 3971 116
Size: 408 1616 758 665 408 860
Fragments arranged by size:
1616 860 758 665 408 408
BsiEI CG_ry'CG
Cuts at: 104 2257 2303 3533 104
Size: 2153 46 1230 1286
Fragments arranged by size:
2153 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 158 224 293 1145 1729 2507 2697 3557 158
Size: 66 69 852 584 778 190 860 1316
Fragments arranged by size:
1316 860 852 778 584 190 69 66
BsmBI CGTCTCn'nnnn_
Cuts at: 775 775
Size: 4715
Bsp1286I G_dGCh'C
Cuts at: 158 224 293 377 383 525 813 1011 1145
Size: 66 69 84 6 142 288 198 134
Cuts at: 1145 1379 1729 2340 2433 2507 2697 2759 3557
Size: 234 350 611 93 74 190 62 798
Cuts at: 3557 158
Size: 1316
Fragments arranged by size:
1316 798 611 350 288 234 198 190
142 134 93 84 74 69 66 62
6
BspHI T'CATG_A
Cuts at: 3147 3147
Size: 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1676 1765 1979 2281 2662 1676
Size: 89 214 302 381 3729
Fragments arranged by size:
3729 381 302 214 89
BsrBI CCG'CTC
Cuts at: 50 891 3007 3061 4545 4710 50
Size: 841 2116 54 1484 165 55
Fragments arranged by size:
2116 1484 841 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2627 2627
Size: 4715
BsrFI r'CCGG_y
Cuts at: 1396 2713 2894 1396
Size: 1317 181 3217
Fragments arranged by size:
3217 1317 181
BssSI C'ACGA_G
Cuts at: 235 295 448 1651 2986 3694 235
Size: 60 153 1203 1335 708 1256
Fragments arranged by size:
1335 1256 1203 708 153 60
BstAPI GCAn_nnn'nTGC
Cuts at: 204 795 1683 2092 204
Size: 591 888 409 2827
Fragments arranged by size:
2827 888 591 409
BstBI TT'CG_AA
Cuts at: 3076 3076
Size: 4715
BstXI CCAn_nnnn'nTGG
Cuts at: 1242 1242
Size: 4715
BstYI r'GATC_y
Cuts at: 1 575 746 1118 1148 1457 1556 1740 2207
Size: 574 171 372 30 309 99 184 467
Cuts at: 2207 2230 2565 2811 3117 3129 3215 4267 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 574 467 449 372 335 309 306
246 184 171 99 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 979 979
Size: 4715
BtgI C'CryG_G
Cuts at: 460 460
Size: 4715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 384 1260 2665 2846 3009 384
Size: 876 1405 181 163 2090
Fragments arranged by size:
2090 1405 876 181 163
BtsI GCAGTG_nn'
Cuts at: 958 1958 958
Size: 1000 3715
DraI TTT'AAA
Cuts at: 1714 1714
Size: 4715
DraIII CAC_nnn'GTG
Cuts at: 1683 1683
Size: 4715
DrdI GACnn_nn'nnGTC
Cuts at: 2421 3765 2421
Size: 1344 3371
EaeI y'GGCC_r
Cuts at: 170 1016 1265 1568 1616 2300 2474 2865 2892
Size: 846 249 303 48 684 174 391 27
Cuts at: 2892 4237 170
Size: 1345 648
Fragments arranged by size:
1345 846 684 648 391 303 249 174
48 27
EagI C'GGCC_G
Cuts at: 2300 2300
Size: 4715
EarI CTCTTCn'nnn_
Cuts at: 163 676 946 2738 2948 163
Size: 513 270 1792 210 1930
Fragments arranged by size:
1930 1792 513 270 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2923 3665 3811 4525 2923
Size: 742 146 714 3113
Fragments arranged by size:
3113 742 714 146
EcoICRI GAG'CTC
Cuts at: 156 1727 156
Size: 1571 3144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 277 605 652 748 1194 1249 1663 2540
Size: 42 328 47 96 446 55 414 877
Cuts at: 2540 2972 3325 3987 235
Size: 432 353 662 963
Fragments arranged by size:
963 877 662 446 432 414 353 328
96 55 47 42
EcoNI CCTnn'n_nnAGG
Cuts at: 943 1630 943
Size: 687 4028
EcoO109I rG'GnC_Cy
Cuts at: 809 810 936 1854 809
Size: 1 126 918 3670
Fragments arranged by size:
3670 918 126 1
EcoRI G'AATT_C
Cuts at: 1719 1719
Size: 4715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 2734 342
Size: 2392 2323
FspI TGC'GCA
Cuts at: 367 2496 367
Size: 2129 2586
FspAI rTGC'GCAy
Cuts at: 367 367
Size: 4715
HaeII r_GCGC'y
Cuts at: 468 1228 1346 1502 2149 2397 3627 4398 468
Size: 760 118 156 647 248 1230 771 785
Fragments arranged by size:
1230 785 771 760 647 248 156 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 326 358 691 708 723 740 940 972 2557
Size: 32 333 17 15 17 200 32 1585
Cuts at: 2557 2587 2589 2619 326
Size: 30 2 30 2422
Fragments arranged by size:
2422 1585 333 200 32 32 30 30
17 17 15 2
HincII GTy'rAC
Cuts at: 1482 1754 1482
Size: 272 4443
KasI G'GCGC_C
Cuts at: 464 1342 1498 2145 2393 464
Size: 878 156 647 248 2786
Fragments arranged by size:
2786 878 647 248 156
KpnI G_GTAC'C
Cuts at: 1735 1735
Size: 4715
MluI A'CGCG_T
Cuts at: 485 4148 485
Size: 3663 1052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 357 3474 3658 357
Size: 3117 184 1414
Fragments arranged by size:
3117 1414 184
MscI TGG'CCA
Cuts at: 1018 1267 1618 2476 1018
Size: 249 351 858 3257
Fragments arranged by size:
3257 858 351 249
MslI CAynn'nnrTG
Cuts at: 2704 2831 2704
Size: 127 4588
MspA1I CmG'CkG
Cuts at: 116 189 462 528 597 792 1826 2140 2500
Size: 73 273 66 69 195 1034 314 360
Cuts at: 2500 3284 3529 116
Size: 784 245 1302
Fragments arranged by size:
1302 1034 784 360 314 273 245 195
73 69 66
NaeI GCC'GGC
Cuts at: 1398 2896 1398
Size: 1498 3217
NarI GG'CG_CC
Cuts at: 465 1343 1499 2146 2394 465
Size: 878 156 647 248 2786
Fragments arranged by size:
2786 878 647 248 156
NgoMIV G'CCGG_C
Cuts at: 1396 2894 1396
Size: 1498 3217
NspI r_CATG'y
Cuts at: 1263 1768 2268 3871 1263
Size: 505 500 1603 2107
Fragments arranged by size:
2107 1603 505 500
PasI CC'CwG_GG
Cuts at: 825 1156 825
Size: 331 4384
PciI A'CATG_T
Cuts at: 3867 3867
Size: 4715
PflMI CCAn_nnn'nTGG
Cuts at: 168 687 168
Size: 519 4196
PfoI T'CCnGG_A
Cuts at: 542 629 542
Size: 87 4628
PmeI GTTT'AAAC
Cuts at: 1714 1714
Size: 4715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 4683
PpuMI rG'GwC_Cy
Cuts at: 936 936
Size: 4715
PspOMI G'GGCC_C
Cuts at: 373 809 373
Size: 436 4279
PstI C_TGCA'G
Cuts at: 1199 1762 2447 1199
Size: 563 685 3467
Fragments arranged by size:
3467 685 563
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4715
PvuII CAG'CTG
Cuts at: 116 189 528 792 2140 2500 116
Size: 73 339 264 1348 360 2331
Fragments arranged by size:
2331 1348 360 339 264 73
RsrII CG'GwC_CG
Cuts at: 2910 2910
Size: 4715
SacI G_AGCT'C
Cuts at: 158 1729 158
Size: 1571 3144
SacII CC_GC'GG
Cuts at: 463 463
Size: 4715
SalI G'TCGA_C
Cuts at: 1752 1752
Size: 4715
SapI GCTCTTCn'nnn_
Cuts at: 163 676 2738 2948 163
Size: 513 2062 210 1930
Fragments arranged by size:
2062 1930 513 210
SbfI CC_TGCA'GG
Cuts at: 1762 1762
Size: 4715
ScaI AGT'ACT
Cuts at: 4232 4232
Size: 4715
SexAI A'CCwGG_T
Cuts at: 1679 1679
Size: 4715
SfcI C'TryA_G
Cuts at: 33 1195 1758 2443 3411 3602 33
Size: 1162 563 685 968 191 1146
Fragments arranged by size:
1162 1146 968 685 563 191
SfoI GGC'GCC
Cuts at: 466 1344 1500 2147 2395 466
Size: 878 156 647 248 2786
Fragments arranged by size:
2786 878 647 248 156
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
SmaI CCC'GGG
Cuts at: 359 573 1737 359
Size: 214 1164 3337
Fragments arranged by size:
3337 1164 214
SmlI C'TyrA_G
Cuts at: 1048 1870 2785 3499 3761 1048
Size: 822 915 714 262 2002
Fragments arranged by size:
2002 915 822 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4715
SphI G_CATG'C
Cuts at: 1768 2268 1768
Size: 500 4215
StyI C'CwwG_G
Cuts at: 1019 1849 2203 1019
Size: 830 354 3531
Fragments arranged by size:
3531 830 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 2298 2431 2890 3099 1413
Size: 885 133 459 209 3029
Fragments arranged by size:
3029 885 459 209 133
TatI w'GTAC_w
Cuts at: 305 4230 305
Size: 3925 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1135 1167 4232 4264 1135
Size: 32 3065 32 1586
Fragments arranged by size:
3065 1586 32 32
Tth111I GACn'n_nGTC
Cuts at: 2512 2512
Size: 4715
XbaI T'CTAG_A
Cuts at: 1746 1746
Size: 4715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 540 1153 1515 2210 426
Size: 114 613 362 695 2931
Fragments arranged by size:
2931 695 613 362 114
XmaI C'CCGG_G
Cuts at: 357 571 1735 357
Size: 214 1164 3337
Fragments arranged by size:
3337 1164 214
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsgI
BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI