(Circular) (Six-base) MAPSORT of: pF1KB3471.seq Check: 2516 from: 1 to: 4715 pF1KB3471 4715 bp With 182 enzymes: SgfI * June 20, 2008 10:23 .. AccI GT'mk_AC Cuts at: 863 1753 863 Size: 890 3825 Acc65I G'GTAC_C Cuts at: 1731 1731 Size: 4715 AclI AA'CG_TT Cuts at: 4555 4555 Size: 4715 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 748 1194 1249 2540 2972 3325 3987 748 Size: 446 55 1291 432 353 662 1476 Fragments arranged by size: 1476 1291 662 446 432 353 55 AflIII A'CryG_T Cuts at: 485 2067 3867 4148 485 Size: 1582 1800 281 1052 Fragments arranged by size: 1800 1582 1052 281 AhdI GACnn_n'nnGTC Cuts at: 954 954 Size: 4715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 682 682 Size: 4715 AlwNI CAG_nnn'CTG Cuts at: 204 792 960 1227 2020 3458 204 Size: 588 168 267 793 1438 1461 Fragments arranged by size: 1461 1438 793 588 267 168 ApaI G_GGCC'C Cuts at: 377 813 377 Size: 436 4279 ApaLI G'TGCA_C Cuts at: 3553 3553 Size: 4715 ApoI r'AATT_y Cuts at: 1719 4068 4324 1719 Size: 2349 256 2110 Fragments arranged by size: 2349 2110 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4715 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4715 AvaI C'yCGr_G Cuts at: 357 376 571 847 1167 1735 357 Size: 19 195 276 320 568 3337 Fragments arranged by size: 3337 568 320 276 195 19 BamHI G'GATC_C Cuts at: 575 1740 575 Size: 1165 3550 BanI G'GyrC_C Cuts at: 432 464 1342 1354 1498 1683 1731 2145 2393 Size: 32 878 12 144 185 48 414 248 Cuts at: 2393 2428 432 Size: 35 2719 Fragments arranged by size: 2719 878 414 248 185 144 48 35 32 12 BanII G_rGCy'C Cuts at: 158 377 383 813 1729 2759 158 Size: 219 6 430 916 1030 2114 Fragments arranged by size: 2114 1030 916 430 219 6 BbeI G_GCGC'C Cuts at: 468 1346 1502 2149 2397 468 Size: 878 156 647 248 2786 Fragments arranged by size: 2786 878 647 248 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 429 463 429 Size: 34 4681 BciVI GTATCCnnnnn_n' Cuts at: 2613 3669 4715 2613 Size: 1056 1046 2613 Fragments arranged by size: 2613 1056 1046 BclI T'GATC_A Cuts at: 716 998 2235 716 Size: 282 1237 3196 Fragments arranged by size: 3196 1237 282 BglI GCCn_nnn'nGGC Cuts at: 1374 1374 Size: 4715 BglII A'GATC_T Cuts at: 1118 1148 1457 1556 2230 4267 1118 Size: 30 309 99 674 2037 1566 Fragments arranged by size: 2037 1566 674 309 99 30 BlpI GC'TnA_GC Cuts at: 1827 1827 Size: 4715 Bme1580I G_kGCm'C Cuts at: 377 525 813 1011 1379 2340 2433 3557 377 Size: 148 288 198 368 961 93 1124 1535 Fragments arranged by size: 1535 1124 961 368 288 198 148 93 BmgBI CAC'GTC Cuts at: 1416 1575 1416 Size: 159 4556 BmrI ACTGGGnnnn_n' Cuts at: 1927 2008 2343 4487 1927 Size: 81 335 2144 2155 Fragments arranged by size: 2155 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 261 3082 261 Size: 2821 1894 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 235 277 605 652 1663 235 Size: 42 328 47 1011 3287 Fragments arranged by size: 3287 1011 328 47 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1033 1891 2770 3484 3782 1033 Size: 858 879 714 298 1966 Fragments arranged by size: 1966 879 858 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 4419 4419 Size: 4715 BsaAI yAC'GTr Cuts at: 2068 2068 Size: 4715 BsaBI GATnn'nnATC Cuts at: 6 367 2229 4266 6 Size: 361 1862 2037 455 Fragments arranged by size: 2037 1862 455 361 BsaHI Gr'CG_yC Cuts at: 465 677 1343 1499 2146 2394 3096 4592 465 Size: 212 666 156 647 248 702 1496 588 Fragments arranged by size: 1496 702 666 647 588 248 212 156 BsaWI w'CCGG_w Cuts at: 1904 2425 3103 3514 3661 3935 1904 Size: 521 678 411 147 274 2684 Fragments arranged by size: 2684 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 856 886 1135 1165 856 Size: 30 249 30 4406 Fragments arranged by size: 4406 249 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 232 283 883 943 1321 1618 232 Size: 51 600 60 378 297 3329 Fragments arranged by size: 3329 600 378 297 60 51 BseYI C'CCAG_C Cuts at: 116 524 2140 2898 3563 3971 116 Size: 408 1616 758 665 408 860 Fragments arranged by size: 1616 860 758 665 408 408 BsiEI CG_ry'CG Cuts at: 104 2257 2303 3533 104 Size: 2153 46 1230 1286 Fragments arranged by size: 2153 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 158 224 293 1145 1729 2507 2697 3557 158 Size: 66 69 852 584 778 190 860 1316 Fragments arranged by size: 1316 860 852 778 584 190 69 66 BsmBI CGTCTCn'nnnn_ Cuts at: 775 775 Size: 4715 Bsp1286I G_dGCh'C Cuts at: 158 224 293 377 383 525 813 1011 1145 Size: 66 69 84 6 142 288 198 134 Cuts at: 1145 1379 1729 2340 2433 2507 2697 2759 3557 Size: 234 350 611 93 74 190 62 798 Cuts at: 3557 158 Size: 1316 Fragments arranged by size: 1316 798 611 350 288 234 198 190 142 134 93 84 74 69 66 62 6 BspHI T'CATG_A Cuts at: 3147 3147 Size: 4715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1676 1765 1979 2281 2662 1676 Size: 89 214 302 381 3729 Fragments arranged by size: 3729 381 302 214 89 BsrBI CCG'CTC Cuts at: 50 891 3007 3061 4545 4710 50 Size: 841 2116 54 1484 165 55 Fragments arranged by size: 2116 1484 841 165 55 54 BsrDI GCAATG_nn' Cuts at: 2627 2627 Size: 4715 BsrFI r'CCGG_y Cuts at: 1396 2713 2894 1396 Size: 1317 181 3217 Fragments arranged by size: 3217 1317 181 BssSI C'ACGA_G Cuts at: 235 295 448 1651 2986 3694 235 Size: 60 153 1203 1335 708 1256 Fragments arranged by size: 1335 1256 1203 708 153 60 BstAPI GCAn_nnn'nTGC Cuts at: 204 795 1683 2092 204 Size: 591 888 409 2827 Fragments arranged by size: 2827 888 591 409 BstBI TT'CG_AA Cuts at: 3076 3076 Size: 4715 BstXI CCAn_nnnn'nTGG Cuts at: 1242 1242 Size: 4715 BstYI r'GATC_y Cuts at: 1 575 746 1118 1148 1457 1556 1740 2207 Size: 574 171 372 30 309 99 184 467 Cuts at: 2207 2230 2565 2811 3117 3129 3215 4267 1 Size: 23 335 246 306 12 86 1052 449 Fragments arranged by size: 1052 574 467 449 372 335 309 306 246 184 171 99 86 30 23 12 Bsu36I CC'TnA_GG Cuts at: 979 979 Size: 4715 BtgI C'CryG_G Cuts at: 460 460 Size: 4715 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 384 1260 2665 2846 3009 384 Size: 876 1405 181 163 2090 Fragments arranged by size: 2090 1405 876 181 163 BtsI GCAGTG_nn' Cuts at: 958 1958 958 Size: 1000 3715 DraI TTT'AAA Cuts at: 1714 1714 Size: 4715 DraIII CAC_nnn'GTG Cuts at: 1683 1683 Size: 4715 DrdI GACnn_nn'nnGTC Cuts at: 2421 3765 2421 Size: 1344 3371 EaeI y'GGCC_r Cuts at: 170 1016 1265 1568 1616 2300 2474 2865 2892 Size: 846 249 303 48 684 174 391 27 Cuts at: 2892 4237 170 Size: 1345 648 Fragments arranged by size: 1345 846 684 648 391 303 249 174 48 27 EagI C'GGCC_G Cuts at: 2300 2300 Size: 4715 EarI CTCTTCn'nnn_ Cuts at: 163 676 946 2738 2948 163 Size: 513 270 1792 210 1930 Fragments arranged by size: 1930 1792 513 270 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2923 3665 3811 4525 2923 Size: 742 146 714 3113 Fragments arranged by size: 3113 742 714 146 EcoICRI GAG'CTC Cuts at: 156 1727 156 Size: 1571 3144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 235 277 605 652 748 1194 1249 1663 2540 Size: 42 328 47 96 446 55 414 877 Cuts at: 2540 2972 3325 3987 235 Size: 432 353 662 963 Fragments arranged by size: 963 877 662 446 432 414 353 328 96 55 47 42 EcoNI CCTnn'n_nnAGG Cuts at: 943 1630 943 Size: 687 4028 EcoO109I rG'GnC_Cy Cuts at: 809 810 936 1854 809 Size: 1 126 918 3670 Fragments arranged by size: 3670 918 126 1 EcoRI G'AATT_C Cuts at: 1719 1719 Size: 4715 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 342 2734 342 Size: 2392 2323 FspI TGC'GCA Cuts at: 367 2496 367 Size: 2129 2586 FspAI rTGC'GCAy Cuts at: 367 367 Size: 4715 HaeII r_GCGC'y Cuts at: 468 1228 1346 1502 2149 2397 3627 4398 468 Size: 760 118 156 647 248 1230 771 785 Fragments arranged by size: 1230 785 771 760 647 248 156 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 326 358 691 708 723 740 940 972 2557 Size: 32 333 17 15 17 200 32 1585 Cuts at: 2557 2587 2589 2619 326 Size: 30 2 30 2422 Fragments arranged by size: 2422 1585 333 200 32 32 30 30 17 17 15 2 HincII GTy'rAC Cuts at: 1482 1754 1482 Size: 272 4443 KasI G'GCGC_C Cuts at: 464 1342 1498 2145 2393 464 Size: 878 156 647 248 2786 Fragments arranged by size: 2786 878 647 248 156 KpnI G_GTAC'C Cuts at: 1735 1735 Size: 4715 MluI A'CGCG_T Cuts at: 485 4148 485 Size: 3663 1052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 357 3474 3658 357 Size: 3117 184 1414 Fragments arranged by size: 3117 1414 184 MscI TGG'CCA Cuts at: 1018 1267 1618 2476 1018 Size: 249 351 858 3257 Fragments arranged by size: 3257 858 351 249 MslI CAynn'nnrTG Cuts at: 2704 2831 2704 Size: 127 4588 MspA1I CmG'CkG Cuts at: 116 189 462 528 597 792 1826 2140 2500 Size: 73 273 66 69 195 1034 314 360 Cuts at: 2500 3284 3529 116 Size: 784 245 1302 Fragments arranged by size: 1302 1034 784 360 314 273 245 195 73 69 66 NaeI GCC'GGC Cuts at: 1398 2896 1398 Size: 1498 3217 NarI GG'CG_CC Cuts at: 465 1343 1499 2146 2394 465 Size: 878 156 647 248 2786 Fragments arranged by size: 2786 878 647 248 156 NgoMIV G'CCGG_C Cuts at: 1396 2894 1396 Size: 1498 3217 NspI r_CATG'y Cuts at: 1263 1768 2268 3871 1263 Size: 505 500 1603 2107 Fragments arranged by size: 2107 1603 505 500 PasI CC'CwG_GG Cuts at: 825 1156 825 Size: 331 4384 PciI A'CATG_T Cuts at: 3867 3867 Size: 4715 PflMI CCAn_nnn'nTGG Cuts at: 168 687 168 Size: 519 4196 PfoI T'CCnGG_A Cuts at: 542 629 542 Size: 87 4628 PmeI GTTT'AAAC Cuts at: 1714 1714 Size: 4715 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3161 3193 3161 Size: 32 4683 PpuMI rG'GwC_Cy Cuts at: 936 936 Size: 4715 PspOMI G'GGCC_C Cuts at: 373 809 373 Size: 436 4279 PstI C_TGCA'G Cuts at: 1199 1762 2447 1199 Size: 563 685 3467 Fragments arranged by size: 3467 685 563 PvuI CG_AT'CG Cuts at: 104 104 Size: 4715 PvuII CAG'CTG Cuts at: 116 189 528 792 2140 2500 116 Size: 73 339 264 1348 360 2331 Fragments arranged by size: 2331 1348 360 339 264 73 RsrII CG'GwC_CG Cuts at: 2910 2910 Size: 4715 SacI G_AGCT'C Cuts at: 158 1729 158 Size: 1571 3144 SacII CC_GC'GG Cuts at: 463 463 Size: 4715 SalI G'TCGA_C Cuts at: 1752 1752 Size: 4715 SapI GCTCTTCn'nnn_ Cuts at: 163 676 2738 2948 163 Size: 513 2062 210 1930 Fragments arranged by size: 2062 1930 513 210 SbfI CC_TGCA'GG Cuts at: 1762 1762 Size: 4715 ScaI AGT'ACT Cuts at: 4232 4232 Size: 4715 SexAI A'CCwGG_T Cuts at: 1679 1679 Size: 4715 SfcI C'TryA_G Cuts at: 33 1195 1758 2443 3411 3602 33 Size: 1162 563 685 968 191 1146 Fragments arranged by size: 1162 1146 968 685 563 191 SfoI GGC'GCC Cuts at: 466 1344 1500 2147 2395 466 Size: 878 156 647 248 2786 Fragments arranged by size: 2786 878 647 248 156 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4715 SmaI CCC'GGG Cuts at: 359 573 1737 359 Size: 214 1164 3337 Fragments arranged by size: 3337 1164 214 SmlI C'TyrA_G Cuts at: 1048 1870 2785 3499 3761 1048 Size: 822 915 714 262 2002 Fragments arranged by size: 2002 915 822 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4715 SphI G_CATG'C Cuts at: 1768 2268 1768 Size: 500 4215 StyI C'CwwG_G Cuts at: 1019 1849 2203 1019 Size: 830 354 3531 Fragments arranged by size: 3531 830 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1413 2298 2431 2890 3099 1413 Size: 885 133 459 209 3029 Fragments arranged by size: 3029 885 459 209 133 TatI w'GTAC_w Cuts at: 305 4230 305 Size: 3925 790 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1135 1167 4232 4264 1135 Size: 32 3065 32 1586 Fragments arranged by size: 3065 1586 32 32 Tth111I GACn'n_nGTC Cuts at: 2512 2512 Size: 4715 XbaI T'CTAG_A Cuts at: 1746 1746 Size: 4715 XcmI CCAnnnn_n'nnnnTGG Cuts at: 426 540 1153 1515 2210 426 Size: 114 613 362 695 2931 Fragments arranged by size: 2931 695 613 362 114 XmaI C'CCGG_G Cuts at: 357 571 1735 357 Size: 214 1164 3337 Fragments arranged by size: 3337 1164 214 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AleI AloI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI