Restriction map for
pF1KB3472
(Circular) (Six-base) MAPSORT of: pF1KB3472.seq Check: 3444 from: 1 to: 5462
pF1KB3472 5462 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1979 2500 1979
Size: 521 4941
Acc65I G'GTAC_C
Cuts at: 2478 2478
Size: 5462
AclI AA'CG_TT
Cuts at: 5302 5302
Size: 5462
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 323 577 710 930 1223 2262 2292 3287
Size: 72 254 133 220 293 1039 30 995
Cuts at: 3287 3719 4072 4734 251
Size: 432 353 662 979
Fragments arranged by size:
1039 995 979 662 432 353 293 254
220 133 72 30
AfeI AGC'GCT
Cuts at: 1987 1987
Size: 5462
AflII C'TTAA_G
Cuts at: 1105 1105
Size: 5462
AflIII A'CryG_T
Cuts at: 2814 4614 4895 2814
Size: 1800 281 3381
Fragments arranged by size:
3381 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 808 897 1467 2767 4205 808
Size: 89 570 1300 1438 2065
Fragments arranged by size:
2065 1438 1300 570 89
ApaLI G'TGCA_C
Cuts at: 4300 4300
Size: 5462
ApoI r'AATT_y
Cuts at: 315 1476 2466 4815 5071 315
Size: 1161 990 2349 256 706
Fragments arranged by size:
2349 1161 990 706 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5462
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
AvaI C'yCGr_G
Cuts at: 2367 2482 2367
Size: 115 5347
BamHI G'GATC_C
Cuts at: 2487 2487
Size: 5462
BanI G'GyrC_C
Cuts at: 1966 2478 2892 3140 3175 1966
Size: 512 414 248 35 4253
Fragments arranged by size:
4253 512 414 248 35
BanII G_rGCy'C
Cuts at: 410 2476 3506 410
Size: 2066 1030 2366
Fragments arranged by size:
2366 2066 1030
BbeI G_GCGC'C
Cuts at: 2896 3144 2896
Size: 248 5214
BbsI GAAGACnn'nnnn_
Cuts at: 820 1211 820
Size: 391 5071
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5428
BciVI GTATCCnnnnn_n'
Cuts at: 3360 4416 5462 3360
Size: 1056 1046 3360
Fragments arranged by size:
3360 1056 1046
BclI T'GATC_A
Cuts at: 1158 1994 2982 1158
Size: 836 988 3638
Fragments arranged by size:
3638 988 836
BglII A'GATC_T
Cuts at: 1092 2977 5014 1092
Size: 1885 2037 1540
Fragments arranged by size:
2037 1885 1540
BlpI GC'TnA_GC
Cuts at: 2574 2574
Size: 5462
Bme1580I G_kGCm'C
Cuts at: 888 3087 3180 4304 888
Size: 2199 93 1124 2046
Fragments arranged by size:
2199 2046 1124 93
BmgBI CAC'GTC
Cuts at: 1973 1973
Size: 5462
BmrI ACTGGGnnnn_n'
Cuts at: 590 818 2674 2755 3090 5234 590
Size: 228 1856 81 335 2144 818
Fragments arranged by size:
2144 1856 818 335 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3829 3829
Size: 5462
Bpu10I CC'TnA_GC
Cuts at: 491 491
Size: 5462
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2638 3517 4231 4529 2638
Size: 879 714 298 3571
Fragments arranged by size:
3571 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5166 5166
Size: 5462
BsaAI yAC'GTr
Cuts at: 1603 2815 1603
Size: 1212 4250
BsaBI GATnn'nnATC
Cuts at: 6 1036 2976 5013 6
Size: 1030 1940 2037 455
Fragments arranged by size:
2037 1940 1030 455
BsaHI Gr'CG_yC
Cuts at: 2893 3141 3843 5339 2893
Size: 248 702 1496 3016
Fragments arranged by size:
3016 1496 702 248
BsaWI w'CCGG_w
Cuts at: 582 2651 3172 3850 4261 4408 4682 582
Size: 2069 521 678 411 147 274 1362
Fragments arranged by size:
2069 1362 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1565 1595 1972 2002 2424 2454 1565
Size: 30 377 30 422 30 4573
Fragments arranged by size:
4573 422 377 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 364 475 734 1930 2110 205
Size: 159 111 259 1196 180 3557
Fragments arranged by size:
3557 1196 259 180 159 111
BseYI C'CCAG_C
Cuts at: 2088 2142 2887 3645 4310 4718 2088
Size: 54 745 758 665 408 2832
Fragments arranged by size:
2832 758 745 665 408 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 1583
Size: 5462
BsiEI CG_ry'CG
Cuts at: 104 3004 3050 4280 104
Size: 2900 46 1230 1286
Fragments arranged by size:
2900 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2038 2476 3254 3444 4304 2038
Size: 438 778 190 860 3196
Fragments arranged by size:
3196 860 778 438 190
Bsp1286I G_dGCh'C
Cuts at: 410 888 2038 2476 3087 3180 3254 3444 3506
Size: 478 1150 438 611 93 74 190 62
Cuts at: 3506 4304 410
Size: 798 1568
Fragments arranged by size:
1568 1150 798 611 478 438 190 93
74 62
BspHI T'CATG_A
Cuts at: 3894 3894
Size: 5462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2512 2726 3028 3409 2512
Size: 214 302 381 4565
Fragments arranged by size:
4565 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3754 3808 5292 5457 50
Size: 3704 54 1484 165 55
Fragments arranged by size:
3704 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 161 1219 3374 161
Size: 1058 2155 2249
Fragments arranged by size:
2249 2155 1058
BsrFI r'CCGG_y
Cuts at: 3460 3641 3460
Size: 181 5281
BssSI C'ACGA_G
Cuts at: 3733 4441 3733
Size: 708 4754
BstAPI GCAn_nnn'nTGC
Cuts at: 2839 2839
Size: 5462
BstBI TT'CG_AA
Cuts at: 3823 3823
Size: 5462
BstYI r'GATC_y
Cuts at: 1 1092 2407 2487 2954 2977 3312 3558 3864
Size: 1091 1315 80 467 23 335 246 306
Cuts at: 3864 3876 3962 5014 1
Size: 12 86 1052 449
Fragments arranged by size:
1315 1091 1052 467 449 335 306 246
86 80 23 12
Bsu36I CC'TnA_GG
Cuts at: 890 1619 890
Size: 729 4733
BtgI C'CryG_G
Cuts at: 2257 2257
Size: 5462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3412 3593 3756 3412
Size: 181 163 5118
Fragments arranged by size:
5118 181 163
BtsI GCAGTG_nn'
Cuts at: 172 1871 2038 2705 172
Size: 1699 167 667 2929
Fragments arranged by size:
2929 1699 667 167
ClaI AT'CG_AT
Cuts at: 1677 1677
Size: 5462
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1319 1354 1319
Size: 35 5427
DraI TTT'AAA
Cuts at: 2461 2461
Size: 5462
DrdI GACnn_nn'nnGTC
Cuts at: 3168 4512 3168
Size: 1344 4118
EaeI y'GGCC_r
Cuts at: 2228 3047 3221 3612 3639 4984 2228
Size: 819 174 391 27 1345 2706
Fragments arranged by size:
2706 1345 819 391 174 27
EagI C'GGCC_G
Cuts at: 3047 3047
Size: 5462
EarI CTCTTCn'nnn_
Cuts at: 227 239 868 1163 3485 3695 227
Size: 12 629 295 2322 210 1994
Fragments arranged by size:
2322 1994 629 295 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1701 3670 4412 4558 5272 1701
Size: 1969 742 146 714 1891
Fragments arranged by size:
1969 1891 742 714 146
EcoICRI GAG'CTC
Cuts at: 2474 2474
Size: 5462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 323 577 710 930 1223 2262 2292 3287
Size: 72 254 133 220 293 1039 30 995
Cuts at: 3287 3719 4072 4734 251
Size: 432 353 662 979
Fragments arranged by size:
1039 995 979 662 432 353 293 254
220 133 72 30
EcoO109I rG'GnC_Cy
Cuts at: 2601 2601
Size: 5462
EcoRI G'AATT_C
Cuts at: 315 1476 2466 315
Size: 1161 990 3311
Fragments arranged by size:
3311 1161 990
EcoRV GAT'ATC
Cuts at: 1038 1038
Size: 5462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3481 3481
Size: 5462
FspI TGC'GCA
Cuts at: 3243 3243
Size: 5462
HaeII r_GCGC'y
Cuts at: 668 1989 2896 3144 4374 5145 668
Size: 1321 907 248 1230 771 985
Fragments arranged by size:
1321 1230 985 907 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 340 372 744 776 1415 1447 1486 1518 1648
Size: 32 372 32 639 32 39 32 130
Cuts at: 1648 1680 1972 2004 3304 3334 3336 3366 340
Size: 32 292 32 1300 30 2 30 2436
Fragments arranged by size:
2436 1300 639 372 292 130 39 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 309 2501 309
Size: 2192 3270
HindIII A'AGCT_T
Cuts at: 1826 1826
Size: 5462
KasI G'GCGC_C
Cuts at: 2892 3140 2892
Size: 248 5214
KpnI G_GTAC'C
Cuts at: 2482 2482
Size: 5462
MfeI C'AATT_G
Cuts at: 2039 2039
Size: 5462
MluI A'CGCG_T
Cuts at: 4895 4895
Size: 5462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1026 1121 4221 4405 1026
Size: 95 3100 184 2083
Fragments arranged by size:
3100 2083 184 95
MscI TGG'CCA
Cuts at: 2230 3223 2230
Size: 993 4469
MslI CAynn'nnrTG
Cuts at: 154 3451 3578 154
Size: 3297 127 2038
Fragments arranged by size:
3297 2038 127
MspA1I CmG'CkG
Cuts at: 773 1900 2088 2289 2319 2349 2573 2887 3247
Size: 1127 188 201 30 30 224 314 360
Cuts at: 3247 4031 4276 773
Size: 784 245 1959
Fragments arranged by size:
1959 1127 784 360 314 245 224 201
188 30 30
NaeI GCC'GGC
Cuts at: 3643 3643
Size: 5462
NarI GG'CG_CC
Cuts at: 2893 3141 2893
Size: 248 5214
NdeI CA'TA_TG
Cuts at: 1287 1287
Size: 5462
NgoMIV G'CCGG_C
Cuts at: 3641 3641
Size: 5462
NspI r_CATG'y
Cuts at: 1114 2515 3015 4618 1114
Size: 1401 500 1603 1958
Fragments arranged by size:
1958 1603 1401 500
PciI A'CATG_T
Cuts at: 4614 4614
Size: 5462
PflMI CCAn_nnn'nTGG
Cuts at: 1140 1140
Size: 5462
PfoI T'CCnGG_A
Cuts at: 1118 1118
Size: 5462
PmeI GTTT'AAAC
Cuts at: 2461 2461
Size: 5462
PmlI CAC'GTG
Cuts at: 1603 1603
Size: 5462
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 3908
Size: 32 5430
PstI C_TGCA'G
Cuts at: 2509 3194 2509
Size: 685 4777
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5462
PvuII CAG'CTG
Cuts at: 1900 2088 2887 3247 1900
Size: 188 799 360 4115
Fragments arranged by size:
4115 799 360 188
RsrII CG'GwC_CG
Cuts at: 3657 3657
Size: 5462
SacI G_AGCT'C
Cuts at: 2476 2476
Size: 5462
SalI G'TCGA_C
Cuts at: 2499 2499
Size: 5462
SapI GCTCTTCn'nnn_
Cuts at: 3485 3695 3485
Size: 210 5252
SbfI CC_TGCA'GG
Cuts at: 2509 2509
Size: 5462
ScaI AGT'ACT
Cuts at: 4979 4979
Size: 5462
SexAI A'CCwGG_T
Cuts at: 1053 1053
Size: 5462
SfcI C'TryA_G
Cuts at: 33 2505 3190 4158 4349 33
Size: 2472 685 968 191 1146
Fragments arranged by size:
2472 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2894 3142 2894
Size: 248 5214
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
SmaI CCC'GGG
Cuts at: 2484 2484
Size: 5462
SmlI C'TyrA_G
Cuts at: 1105 2617 3532 4246 4508 1105
Size: 1512 915 714 262 2059
Fragments arranged by size:
2059 1512 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5462
SphI G_CATG'C
Cuts at: 2515 3015 2515
Size: 500 4962
SspI AAT'ATT
Cuts at: 2055 2055
Size: 5462
StyI C'CwwG_G
Cuts at: 2596 2950 2596
Size: 354 5108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2134 3045 3178 3637 3846 2134
Size: 911 133 459 209 3750
Fragments arranged by size:
3750 911 459 209 133
TatI w'GTAC_w
Cuts at: 1755 4977 1755
Size: 3222 2240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1565 1597 4979 5011 1565
Size: 32 3382 32 2016
Fragments arranged by size:
3382 2016 32 32
Tth111I GACn'n_nGTC
Cuts at: 3259 3259
Size: 5462
XbaI T'CTAG_A
Cuts at: 2493 2493
Size: 5462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1078 1851 2957 1078
Size: 773 1106 3583
Fragments arranged by size:
3583 1106 773
XmaI C'CCGG_G
Cuts at: 2482 2482
Size: 5462
XmnI GAAnn'nnTTC
Cuts at: 817 817
Size: 5462
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BpmI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I DraIII EcoNI FseI FspAI HpaI NcoI NheI
NotI NruI NsiI PacI PasI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI