Restriction map for
pF1KB3473
(Circular) (Six-base) MAPSORT of: pF1KB3473.seq Check: 5903 from: 1 to: 4604
pF1KB3473 4604 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 706 706
Size: 4604
AccI GT'mk_AC
Cuts at: 313 1642 313
Size: 1329 3275
Acc65I G'GTAC_C
Cuts at: 1620 1620
Size: 4604
AclI AA'CG_TT
Cuts at: 619 4444 619
Size: 3825 779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2429 2861 3214 3876 2429
Size: 432 353 662 3157
Fragments arranged by size:
3157 662 432 353
AfeI AGC'GCT
Cuts at: 1110 1110
Size: 4604
AflIII A'CryG_T
Cuts at: 1586 1956 3756 4037 1586
Size: 370 1800 281 2153
Fragments arranged by size:
2153 1800 370 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 868 868
Size: 4604
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 969 1001 969
Size: 32 4572
AlwNI CAG_nnn'CTG
Cuts at: 1909 3347 1909
Size: 1438 3166
ApaLI G'TGCA_C
Cuts at: 3442 3442
Size: 4604
ApoI r'AATT_y
Cuts at: 810 1608 3957 4213 810
Size: 798 2349 256 1201
Fragments arranged by size:
2349 1201 798 256
AseI AT'TA_AT
Cuts at: 20 190 1259 20
Size: 170 1069 3365
Fragments arranged by size:
3365 1069 170
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
AvaI C'yCGr_G
Cuts at: 1307 1624 1307
Size: 317 4287
AvrII C'CTAG_G
Cuts at: 1191 1191
Size: 4604
BamHI G'GATC_C
Cuts at: 1491 1629 1491
Size: 138 4466
BanI G'GyrC_C
Cuts at: 1620 2034 2282 2317 1620
Size: 414 248 35 3907
Fragments arranged by size:
3907 414 248 35
BanII G_rGCy'C
Cuts at: 403 687 1618 2648 403
Size: 284 931 1030 2359
Fragments arranged by size:
2359 1030 931 284
BbeI G_GCGC'C
Cuts at: 2038 2286 2038
Size: 248 4356
BbvCI CC'TCA_GC
Cuts at: 1207 1207
Size: 4604
BciVI GTATCCnnnnn_n'
Cuts at: 2502 3558 4604 2502
Size: 1056 1046 2502
Fragments arranged by size:
2502 1056 1046
BclI T'GATC_A
Cuts at: 2124 2124
Size: 4604
BglII A'GATC_T
Cuts at: 915 2119 4156 915
Size: 1204 2037 1363
Fragments arranged by size:
2037 1363 1204
BlpI GC'TnA_GC
Cuts at: 1716 1716
Size: 4604
Bme1580I G_kGCm'C
Cuts at: 2229 2322 3446 2229
Size: 93 1124 3387
Fragments arranged by size:
3387 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1816 1897 2232 4376 1009
Size: 807 81 335 2144 1237
Fragments arranged by size:
2144 1237 807 335 81
BmtI G_CTAG'C
Cuts at: 284 284
Size: 4604
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 1244 701
Size: 543 4061
Bpu10I CC'TnA_GC
Cuts at: 840 1207 840
Size: 367 4237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 780 974 1780 2659 3373 3671 292
Size: 488 194 806 879 714 298 1225
Fragments arranged by size:
1225 879 806 714 488 298 194
BsaI GGTCTCn'nnnn_
Cuts at: 687 4308 687
Size: 3621 983
BsaAI yAC'GTr
Cuts at: 1957 1957
Size: 4604
BsaBI GATnn'nnATC
Cuts at: 6 2118 4155 6
Size: 2112 2037 455
Fragments arranged by size:
2112 2037 455
BsaHI Gr'CG_yC
Cuts at: 2035 2283 2985 4481 2035
Size: 248 702 1496 2158
Fragments arranged by size:
2158 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1793 2314 2992 3403 3550 3824 1793
Size: 521 678 411 147 274 2573
Fragments arranged by size:
2573 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 849 879 849
Size: 30 4574
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 677 1295 677
Size: 618 3986
BseYI C'CCAG_C
Cuts at: 1158 1388 1409 2029 2787 3452 3860 1158
Size: 230 21 620 758 665 408 1902
Fragments arranged by size:
1902 758 665 620 408 230 21
BsiEI CG_ry'CG
Cuts at: 104 2146 2192 3422 104
Size: 2042 46 1230 1286
Fragments arranged by size:
2042 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 221 428 687 733 1618 2396 2586 3446 221
Size: 207 259 46 885 778 190 860 1379
Fragments arranged by size:
1379 885 860 778 259 207 190 46
BsmI GAATG_Cn'
Cuts at: 1154 1154
Size: 4604
Bsp1286I G_dGCh'C
Cuts at: 221 403 428 687 733 1618 2229 2322 2396
Size: 182 25 259 46 885 611 93 74
Cuts at: 2396 2586 2648 3446 221
Size: 190 62 798 1379
Fragments arranged by size:
1379 885 798 611 259 190 182 93
74 62 46 25
BspHI T'CATG_A
Cuts at: 333 3036 333
Size: 2703 1901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 1654 1868 2170 2551 706
Size: 948 214 302 381 2759
Fragments arranged by size:
2759 948 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2896 2950 4434 4599 50
Size: 2846 54 1484 165 55
Fragments arranged by size:
2846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 1076 2516 717
Size: 359 1440 2805
Fragments arranged by size:
2805 1440 359
BsrFI r'CCGG_y
Cuts at: 2602 2783 2602
Size: 181 4423
BssSI C'ACGA_G
Cuts at: 2875 3583 2875
Size: 708 3896
BstAPI GCAn_nnn'nTGC
Cuts at: 1981 1981
Size: 4604
BstBI TT'CG_AA
Cuts at: 2965 2965
Size: 4604
BstEII G'GTnAC_C
Cuts at: 1593 1593
Size: 4604
BstYI r'GATC_y
Cuts at: 1 915 1491 1629 2096 2119 2454 2700 3006
Size: 914 576 138 467 23 335 246 306
Cuts at: 3006 3018 3104 4156 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 914 576 467 449 335 306 246
138 86 23 12
BstZ17I GTA'TAC
Cuts at: 314 314
Size: 4604
BtgI C'CryG_G
Cuts at: 107 1495 107
Size: 1388 3216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2735 2898 2554
Size: 181 163 4260
Fragments arranged by size:
4260 181 163
BtsI GCAGTG_nn'
Cuts at: 1485 1847 1485
Size: 362 4242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 699 734 699
Size: 35 4569
DraI TTT'AAA
Cuts at: 1603 1603
Size: 4604
DraIII CAC_nnn'GTG
Cuts at: 880 880
Size: 4604
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3654 2310
Size: 1344 3260
EaeI y'GGCC_r
Cuts at: 1527 2189 2363 2754 2781 4126 1527
Size: 662 174 391 27 1345 2005
Fragments arranged by size:
2005 1345 662 391 174 27
EagI C'GGCC_G
Cuts at: 2189 2189
Size: 4604
EarI CTCTTCn'nnn_
Cuts at: 2627 2837 2627
Size: 210 4394
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 129 2812 3554 3700 4414 129
Size: 2683 742 146 714 319
Fragments arranged by size:
2683 742 714 319 146
EcoICRI GAG'CTC
Cuts at: 685 1616 685
Size: 931 3673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 1244 2429 2861 3214 3876 701
Size: 543 1185 432 353 662 1429
Fragments arranged by size:
1429 1185 662 543 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 875 1049 875
Size: 174 4430
EcoO109I rG'GnC_Cy
Cuts at: 493 1743 493
Size: 1250 3354
EcoRI G'AATT_C
Cuts at: 1608 1608
Size: 4604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4604
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4604
HaeII r_GCGC'y
Cuts at: 1112 1434 2038 2286 3516 4287 1112
Size: 322 604 248 1230 771 1429
Fragments arranged by size:
1429 1230 771 604 322 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 914 946 1287 1319 2446 2476 2478 2508 914
Size: 32 341 32 1127 30 2 30 3010
Fragments arranged by size:
3010 1127 341 32 32 30 30 2
HincII GTy'rAC
Cuts at: 623 1643 623
Size: 1020 3584
HindIII A'AGCT_T
Cuts at: 1038 1038
Size: 4604
HpaI GTT'AAC
Cuts at: 623 623
Size: 4604
KasI G'GCGC_C
Cuts at: 2034 2282 2034
Size: 248 4356
KpnI G_GTAC'C
Cuts at: 1624 1624
Size: 4604
MfeI C'AATT_G
Cuts at: 1517 1517
Size: 4604
MluI A'CGCG_T
Cuts at: 1586 4037 1586
Size: 2451 2153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3363 3547 3363
Size: 184 4420
MscI TGG'CCA
Cuts at: 2365 2365
Size: 4604
MslI CAynn'nnrTG
Cuts at: 289 668 2593 2720 289
Size: 379 1925 127 2173
Fragments arranged by size:
2173 1925 379 127
MspA1I CmG'CkG
Cuts at: 1715 2029 2389 3173 3418 1715
Size: 314 360 784 245 2901
Fragments arranged by size:
2901 784 360 314 245
NaeI GCC'GGC
Cuts at: 2785 2785
Size: 4604
NarI GG'CG_CC
Cuts at: 2035 2283 2035
Size: 248 4356
NcoI C'CATG_G
Cuts at: 107 107
Size: 4604
NgoMIV G'CCGG_C
Cuts at: 2783 2783
Size: 4604
NheI G'CTAG_C
Cuts at: 280 280
Size: 4604
NspI r_CATG'y
Cuts at: 639 1657 2157 3760 639
Size: 1018 500 1603 1483
Fragments arranged by size:
1603 1483 1018 500
PasI CC'CwG_GG
Cuts at: 577 577
Size: 4604
PciI A'CATG_T
Cuts at: 3756 3756
Size: 4604
PflMI CCAn_nnn'nTGG
Cuts at: 706 880 883 706
Size: 174 3 4427
Fragments arranged by size:
4427 174 3
PfoI T'CCnGG_A
Cuts at: 678 678
Size: 4604
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 4572
PshAI GACnn'nnGTC
Cuts at: 1167 1167
Size: 4604
PstI C_TGCA'G
Cuts at: 1651 2336 1651
Size: 685 3919
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4604
PvuII CAG'CTG
Cuts at: 2029 2389 2029
Size: 360 4244
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4604
SacI G_AGCT'C
Cuts at: 687 1618 687
Size: 931 3673
SalI G'TCGA_C
Cuts at: 1641 1641
Size: 4604
SapI GCTCTTCn'nnn_
Cuts at: 2627 2837 2627
Size: 210 4394
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4604
ScaI AGT'ACT
Cuts at: 705 4121 705
Size: 3416 1188
SexAI A'CCwGG_T
Cuts at: 876 876
Size: 4604
SfcI C'TryA_G
Cuts at: 33 734 1647 2332 3300 3491 33
Size: 701 913 685 968 191 1146
Fragments arranged by size:
1146 968 913 701 685 191
SfoI GGC'GCC
Cuts at: 2036 2284 2036
Size: 248 4356
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
SmaI CCC'GGG
Cuts at: 1626 1626
Size: 4604
SmlI C'TyrA_G
Cuts at: 307 795 989 1759 2674 3388 3650 307
Size: 488 194 770 915 714 262 1261
Fragments arranged by size:
1261 915 770 714 488 262 194
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4604
SphI G_CATG'C
Cuts at: 639 1657 2157 639
Size: 1018 500 3086
Fragments arranged by size:
3086 1018 500
StuI AGG'CCT
Cuts at: 383 383
Size: 4604
StyI C'CwwG_G
Cuts at: 107 1191 1738 2092 107
Size: 1084 547 354 2619
Fragments arranged by size:
2619 1084 547 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 985 1581 2187 2320 2779 2988 985
Size: 596 606 133 459 209 2601
Fragments arranged by size:
2601 606 596 459 209 133
TatI w'GTAC_w
Cuts at: 703 1061 4119 703
Size: 358 3058 1188
Fragments arranged by size:
3058 1188 358
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 4572
Tth111I GACn'n_nGTC
Cuts at: 2401 2401
Size: 4604
XbaI T'CTAG_A
Cuts at: 1635 1635
Size: 4604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 2099
Size: 4604
XmaI C'CCGG_G
Cuts at: 1624 1624
Size: 4604
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI ApaI AscI BaeI
BbsI BcgI BfrBI BglI BmgBI BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstXI Bsu36I ClaI EcoRV FseI
FspAI NdeI NotI NruI NsiI PacI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI