Restriction map for
pF1KB3475
(Circular) (Six-base) MAPSORT of: pF1KB3475.seq Check: 615 from: 1 to: 4589
pF1KB3475 4589 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1627 1627
Size: 4589
Acc65I G'GTAC_C
Cuts at: 1605 1605
Size: 4589
AclI AA'CG_TT
Cuts at: 4429 4429
Size: 4589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1275 2414 2846 3199 3861 1275
Size: 1139 432 353 662 2003
Fragments arranged by size:
2003 1139 662 432 353
AflIII A'CryG_T
Cuts at: 1941 3741 4022 1941
Size: 1800 281 2508
Fragments arranged by size:
2508 1800 281
AleI CACnn'nnGTG
Cuts at: 1072 1072
Size: 4589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 4589
AlwNI CAG_nnn'CTG
Cuts at: 957 1894 3332 957
Size: 937 1438 2214
Fragments arranged by size:
2214 1438 937
ApaI G_GGCC'C
Cuts at: 573 573
Size: 4589
ApaLI G'TGCA_C
Cuts at: 3427 3427
Size: 4589
ApoI r'AATT_y
Cuts at: 387 1350 1593 3942 4198 387
Size: 963 243 2349 256 778
Fragments arranged by size:
2349 963 778 256 243
AseI AT'TA_AT
Cuts at: 20 20
Size: 4589
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
AvaI C'yCGr_G
Cuts at: 730 1543 1609 730
Size: 813 66 3710
Fragments arranged by size:
3710 813 66
BamHI G'GATC_C
Cuts at: 1614 1614
Size: 4589
BanI G'GyrC_C
Cuts at: 577 1605 2019 2267 2302 577
Size: 1028 414 248 35 2864
Fragments arranged by size:
2864 1028 414 248 35
BanII G_rGCy'C
Cuts at: 116 573 1603 2633 116
Size: 457 1030 1030 2072
Fragments arranged by size:
2072 1030 1030 457
BbeI G_GCGC'C
Cuts at: 2023 2271 2023
Size: 248 4341
BbsI GAAGACnn'nnnn_
Cuts at: 969 1398 969
Size: 429 4160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 585 597 619 631 585
Size: 12 22 12 4543
Fragments arranged by size:
4543 22 12 12
BciVI GTATCCnnnnn_n'
Cuts at: 2487 3543 4589 2487
Size: 1056 1046 2487
Fragments arranged by size:
2487 1056 1046
BclI T'GATC_A
Cuts at: 2109 2109
Size: 4589
BglI GCCn_nnn'nGGC
Cuts at: 1133 1133
Size: 4589
BglII A'GATC_T
Cuts at: 2104 4141 2104
Size: 2037 2552
BlpI GC'TnA_GC
Cuts at: 676 1701 676
Size: 1025 3564
Bme1580I G_kGCm'C
Cuts at: 530 573 580 2214 2307 3431 530
Size: 43 7 1634 93 1124 1688
Fragments arranged by size:
1688 1634 1124 93 43 7
BmgBI CAC'GTC
Cuts at: 816 1306 816
Size: 490 4099
BmrI ACTGGGnnnn_n'
Cuts at: 460 1801 1882 2217 4361 460
Size: 1341 81 335 2144 688
Fragments arranged by size:
2144 1341 688 335 81
BmtI G_CTAG'C
Cuts at: 1174 1174
Size: 4589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 907 1201 218
Size: 689 294 3606
Fragments arranged by size:
3606 689 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1765 2644 3358 3656 1765
Size: 879 714 298 2698
Fragments arranged by size:
2698 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4293 4293
Size: 4589
BsaAI yAC'GTr
Cuts at: 1942 1942
Size: 4589
BsaBI GATnn'nnATC
Cuts at: 6 472 2103 4140 6
Size: 466 1631 2037 455
Fragments arranged by size:
2037 1631 466 455
BsaHI Gr'CG_yC
Cuts at: 2020 2268 2970 4466 2020
Size: 248 702 1496 2143
Fragments arranged by size:
2143 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1045 1778 2299 2977 3388 3535 3809 1045
Size: 733 521 678 411 147 274 1825
Fragments arranged by size:
1825 733 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 149 179 1375 1405 149
Size: 30 1196 30 3333
Fragments arranged by size:
3333 1196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 173 244 781 1561 173
Size: 71 537 780 3201
Fragments arranged by size:
3201 780 537 71
BseYI C'CCAG_C
Cuts at: 115 591 950 2014 2772 3437 3845 115
Size: 476 359 1064 758 665 408 859
Fragments arranged by size:
1064 859 758 665 476 408 359
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1036 568
Size: 468 4121
BsiEI CG_ry'CG
Cuts at: 104 2131 2177 3407 104
Size: 2027 46 1230 1286
Fragments arranged by size:
2027 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 434 1427 1603 2381 2571 3431 434
Size: 993 176 778 190 860 1592
Fragments arranged by size:
1592 993 860 778 190 176
Bsp1286I G_dGCh'C
Cuts at: 116 434 530 573 580 1427 1603 2214 2307
Size: 318 96 43 7 847 176 611 93
Cuts at: 2307 2381 2571 2633 3431 116
Size: 74 190 62 798 1274
Fragments arranged by size:
1274 847 798 611 318 190 176 96
93 74 62 43 7
BspHI T'CATG_A
Cuts at: 1157 3021 1157
Size: 1864 2725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 416 836 1639 1853 2155 2536 416
Size: 420 803 214 302 381 2469
Fragments arranged by size:
2469 803 420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 899 2881 2935 4419 4584 50
Size: 849 1982 54 1484 165 55
Fragments arranged by size:
1982 1484 849 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1316 2501 1316
Size: 1185 3404
BsrFI r'CCGG_y
Cuts at: 380 1103 2587 2768 380
Size: 723 1484 181 2201
Fragments arranged by size:
2201 1484 723 181
BssSI C'ACGA_G
Cuts at: 990 2860 3568 990
Size: 1870 708 2011
Fragments arranged by size:
2011 1870 708
BstAPI GCAn_nnn'nTGC
Cuts at: 861 1966 861
Size: 1105 3484
BstBI TT'CG_AA
Cuts at: 2950 2950
Size: 4589
BstEII G'GTnAC_C
Cuts at: 876 876
Size: 4589
BstXI CCAn_nnnn'nTGG
Cuts at: 262 537 262
Size: 275 4314
BstYI r'GATC_y
Cuts at: 1 1614 2081 2104 2439 2685 2991 3003 3089
Size: 1613 467 23 335 246 306 12 86
Cuts at: 3089 4141 1
Size: 1052 449
Fragments arranged by size:
1613 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 152 1267 107
Size: 45 1115 3429
Fragments arranged by size:
3429 1115 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 600 912 1149 2539 2720 2883 600
Size: 312 237 1390 181 163 2306
Fragments arranged by size:
2306 1390 312 237 181 163
BtsI GCAGTG_nn'
Cuts at: 170 712 1174 1832 170
Size: 542 462 658 2927
Fragments arranged by size:
2927 658 542 462
DraI TTT'AAA
Cuts at: 1588 1588
Size: 4589
DrdI GACnn_nn'nnGTC
Cuts at: 2295 3639 2295
Size: 1344 3245
EaeI y'GGCC_r
Cuts at: 149 314 378 1217 2174 2348 2739 2766 4111
Size: 165 64 839 957 174 391 27 1345
Cuts at: 4111 149
Size: 627
Fragments arranged by size:
1345 957 839 627 391 174 165 64
27
EagI C'GGCC_G
Cuts at: 2174 2174
Size: 4589
EarI CTCTTCn'nnn_
Cuts at: 839 1294 2612 2822 839
Size: 455 1318 210 2606
Fragments arranged by size:
2606 1318 455 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 399 414 1122 2797 3539 3685 4399 399
Size: 15 708 1675 742 146 714 589
Fragments arranged by size:
1675 742 714 708 589 146 15
EcoICRI GAG'CTC
Cuts at: 1601 1601
Size: 4589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 907 1201 1275 2414 2846 3199 3861 218
Size: 689 294 74 1139 432 353 662 946
Fragments arranged by size:
1139 946 689 662 432 353 294 74
EcoO109I rG'GnC_Cy
Cuts at: 569 570 657 1252 1728 569
Size: 1 87 595 476 3430
Fragments arranged by size:
3430 595 476 87 1
EcoRI G'AATT_C
Cuts at: 387 1593 387
Size: 1206 3383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4589
FspI TGC'GCA
Cuts at: 2370 2370
Size: 4589
HaeII r_GCGC'y
Cuts at: 2023 2271 3501 4272 2023
Size: 248 1230 771 2340
Fragments arranged by size:
2340 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1101 1133 1188 1220 2431 2461 2463 2493 1101
Size: 32 55 32 1211 30 2 30 3197
Fragments arranged by size:
3197 1211 55 32 32 30 30 2
HincII GTy'rAC
Cuts at: 369 1056 1628 369
Size: 687 572 3330
Fragments arranged by size:
3330 687 572
HpaI GTT'AAC
Cuts at: 369 369
Size: 4589
KasI G'GCGC_C
Cuts at: 2019 2267 2019
Size: 248 4341
KpnI G_GTAC'C
Cuts at: 1609 1609
Size: 4589
MluI A'CGCG_T
Cuts at: 4022 4022
Size: 4589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1361 3348 3532 1361
Size: 1987 184 2418
Fragments arranged by size:
2418 1987 184
MscI TGG'CCA
Cuts at: 316 1219 2350 316
Size: 903 1131 2555
Fragments arranged by size:
2555 1131 903
MslI CAynn'nnrTG
Cuts at: 260 496 535 931 1072 1156 2578 2705 260
Size: 236 39 396 141 84 1422 127 2144
Fragments arranged by size:
2144 1422 396 236 141 127 84 39
MspA1I CmG'CkG
Cuts at: 591 811 945 957 1312 1700 2014 2374 3158
Size: 220 134 12 355 388 314 360 784
Cuts at: 3158 3403 591
Size: 245 1777
Fragments arranged by size:
1777 784 388 360 355 314 245 220
134 12
NaeI GCC'GGC
Cuts at: 382 1105 2770 382
Size: 723 1665 2201
Fragments arranged by size:
2201 1665 723
NarI GG'CG_CC
Cuts at: 2020 2268 2020
Size: 248 4341
NcoI C'CATG_G
Cuts at: 107 1267 107
Size: 1160 3429
NgoMIV G'CCGG_C
Cuts at: 380 1103 2768 380
Size: 723 1665 2201
Fragments arranged by size:
2201 1665 723
NheI G'CTAG_C
Cuts at: 1170 1170
Size: 4589
NspI r_CATG'y
Cuts at: 1368 1642 2142 3745 1368
Size: 274 500 1603 2212
Fragments arranged by size:
2212 1603 500 274
PasI CC'CwG_GG
Cuts at: 573 1563 573
Size: 990 3599
PciI A'CATG_T
Cuts at: 3741 3741
Size: 4589
PflMI CCAn_nnn'nTGG
Cuts at: 299 1268 1380 1563 299
Size: 969 112 183 3325
Fragments arranged by size:
3325 969 183 112
PfoI T'CCnGG_A
Cuts at: 884 884
Size: 4589
PmeI GTTT'AAAC
Cuts at: 1588 1588
Size: 4589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1537 1569 3035 3067 1537
Size: 32 1466 32 3059
Fragments arranged by size:
3059 1466 32 32
PpuMI rG'GwC_Cy
Cuts at: 657 1252 657
Size: 595 3994
PshAI GACnn'nnGTC
Cuts at: 250 250
Size: 4589
PspOMI G'GGCC_C
Cuts at: 569 569
Size: 4589
PstI C_TGCA'G
Cuts at: 182 707 833 944 1636 2321 182
Size: 525 126 111 692 685 2450
Fragments arranged by size:
2450 692 685 525 126 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4589
PvuII CAG'CTG
Cuts at: 591 811 945 957 1312 2014 2374 591
Size: 220 134 12 355 702 360 2806
Fragments arranged by size:
2806 702 360 355 220 134 12
RsrII CG'GwC_CG
Cuts at: 2784 2784
Size: 4589
SacI G_AGCT'C
Cuts at: 1603 1603
Size: 4589
SalI G'TCGA_C
Cuts at: 1626 1626
Size: 4589
SapI GCTCTTCn'nnn_
Cuts at: 2612 2822 2612
Size: 210 4379
SbfI CC_TGCA'GG
Cuts at: 833 1636 833
Size: 803 3786
ScaI AGT'ACT
Cuts at: 4106 4106
Size: 4589
SfcI C'TryA_G
Cuts at: 33 178 703 829 940 1632 2317 3285 3476
Size: 145 525 126 111 692 685 968 191
Cuts at: 3476 33
Size: 1146
Fragments arranged by size:
1146 968 692 685 525 191 145 126
111
SfoI GGC'GCC
Cuts at: 2021 2269 2021
Size: 248 4341
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
SmaI CCC'GGG
Cuts at: 1611 1611
Size: 4589
SmlI C'TyrA_G
Cuts at: 1744 2659 3373 3635 1744
Size: 915 714 262 2698
Fragments arranged by size:
2698 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4589
SphI G_CATG'C
Cuts at: 1642 2142 1642
Size: 500 4089
StuI AGG'CCT
Cuts at: 1127 1127
Size: 4589
StyI C'CwwG_G
Cuts at: 107 1267 1723 2077 107
Size: 1160 456 354 2619
Fragments arranged by size:
2619 1160 456 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 567 2172 2305 2764 2973 529
Size: 38 1605 133 459 209 2145
Fragments arranged by size:
2145 1605 459 209 133 38
TatI w'GTAC_w
Cuts at: 500 4104 500
Size: 3604 985
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 149 181 1375 1407 4106 4138 149
Size: 32 1194 32 2699 32 600
Fragments arranged by size:
2699 1194 600 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2386 2386
Size: 4589
XbaI T'CTAG_A
Cuts at: 1620 1620
Size: 4589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 958 2084 958
Size: 1126 3463
XmaI C'CCGG_G
Cuts at: 1609 1609
Size: 4589
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NotI
NruI NsiI PacI PmlI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI