Restriction map for
pF1KB3476
(Circular) (Six-base) MAPSORT of: pF1KB3476.seq Check: 6890 from: 1 to: 5282
pF1KB3476 5282 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 2320 2320
Size: 5282
Acc65I G'GTAC_C
Cuts at: 1769 2298 1769
Size: 529 4753
AclI AA'CG_TT
Cuts at: 5122 5122
Size: 5282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 3107 3539 3892 4554 119
Size: 2988 432 353 662 847
Fragments arranged by size:
2988 847 662 432 353
AflIII A'CryG_T
Cuts at: 274 665 1292 2634 4434 4715 274
Size: 391 627 1342 1800 281 841
Fragments arranged by size:
1800 1342 841 627 391 281
AleI CACnn'nnGTG
Cuts at: 575 1778 575
Size: 1203 4079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 593 593
Size: 5282
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1282 1314 1282
Size: 32 5250
AlwNI CAG_nnn'CTG
Cuts at: 2587 4025 2587
Size: 1438 3844
ApaLI G'TGCA_C
Cuts at: 4120 4120
Size: 5282
ApoI r'AATT_y
Cuts at: 945 995 1180 1618 1681 2041 2272 2286 4635
Size: 50 185 438 63 360 231 14 2349
Cuts at: 4635 4891 945
Size: 256 1336
Fragments arranged by size:
2349 1336 438 360 256 231 185 63
50 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 5282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
AvaI C'yCGr_G
Cuts at: 1263 1466 2302 1263
Size: 203 836 4243
Fragments arranged by size:
4243 836 203
BamHI G'GATC_C
Cuts at: 2064 2307 2064
Size: 243 5039
BanI G'GyrC_C
Cuts at: 1769 2298 2712 2960 2995 1769
Size: 529 414 248 35 4056
Fragments arranged by size:
4056 529 414 248 35
BanII G_rGCy'C
Cuts at: 2296 3326 2296
Size: 1030 4252
BbeI G_GCGC'C
Cuts at: 2716 2964 2716
Size: 248 5034
BciVI GTATCCnnnnn_n'
Cuts at: 3180 4236 5282 3180
Size: 1056 1046 3180
Fragments arranged by size:
3180 1056 1046
BclI T'GATC_A
Cuts at: 1368 2802 1368
Size: 1434 3848
BglI GCCn_nnn'nGGC
Cuts at: 1404 1950 1404
Size: 546 4736
BglII A'GATC_T
Cuts at: 882 2797 4834 882
Size: 1915 2037 1330
Fragments arranged by size:
2037 1915 1330
BlpI GC'TnA_GC
Cuts at: 2394 2394
Size: 5282
Bme1580I G_kGCm'C
Cuts at: 2907 3000 4124 2907
Size: 93 1124 4065
Fragments arranged by size:
4065 1124 93
BmgBI CAC'GTC
Cuts at: 277 277
Size: 5282
BmrI ACTGGGnnnn_n'
Cuts at: 969 2494 2575 2910 5054 969
Size: 1525 81 335 2144 1197
Fragments arranged by size:
2144 1525 1197 335 81
BmtI G_CTAG'C
Cuts at: 1411 1411
Size: 5282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1722 3649 1722
Size: 1927 3355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 733 973 1466 1730 245
Size: 488 240 493 264 3797
Fragments arranged by size:
3797 493 488 264 240
Bpu10I CC'TnA_GC
Cuts at: 554 554
Size: 5282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 517 2458 3337 4051 4349 301
Size: 216 1941 879 714 298 1234
Fragments arranged by size:
1941 1234 879 714 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 4986 4986
Size: 5282
BsaAI yAC'GTr
Cuts at: 956 1558 2635 956
Size: 602 1077 3603
Fragments arranged by size:
3603 1077 602
BsaBI GATnn'nnATC
Cuts at: 6 1912 2063 2796 4833 6
Size: 1906 151 733 2037 455
Fragments arranged by size:
2037 1906 733 455 151
BsaHI Gr'CG_yC
Cuts at: 2713 2961 3663 5159 2713
Size: 248 702 1496 2836
Fragments arranged by size:
2836 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2471 2992 3670 4081 4228 4502 2471
Size: 521 678 411 147 274 3251
Fragments arranged by size:
3251 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 706 736 1282 1312 706
Size: 30 546 30 4676
Fragments arranged by size:
4676 546 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 169 130
Size: 39 5243
BseYI C'CCAG_C
Cuts at: 2707 3465 4130 4538 2707
Size: 758 665 408 3451
Fragments arranged by size:
3451 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2824 2870 4100 104
Size: 2720 46 1230 1286
Fragments arranged by size:
2720 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 582 1452 2296 3074 3264 4124 582
Size: 870 844 778 190 860 1740
Fragments arranged by size:
1740 870 860 844 778 190
BsiWI C'GTAC_G
Cuts at: 1558 1558
Size: 5282
Bsp1286I G_dGCh'C
Cuts at: 582 1452 2296 2907 3000 3074 3264 3326 4124
Size: 870 844 611 93 74 190 62 798
Cuts at: 4124 582
Size: 1740
Fragments arranged by size:
1740 870 844 798 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 1113 3714 1113
Size: 2601 2681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2332 2546 2848 3229 2332
Size: 214 302 381 4385
Fragments arranged by size:
4385 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2226 3574 3628 5112 5277 50
Size: 2176 1348 54 1484 165 55
Fragments arranged by size:
2176 1484 1348 165 55 54
BsrDI GCAATG_nn'
Cuts at: 702 797 3194 702
Size: 95 2397 2790
Fragments arranged by size:
2790 2397 95
BsrFI r'CCGG_y
Cuts at: 3280 3461 3280
Size: 181 5101
BsrGI T'GTAC_A
Cuts at: 2074 2074
Size: 5282
BssSI C'ACGA_G
Cuts at: 3553 4261 3553
Size: 708 4574
BstAPI GCAn_nnn'nTGC
Cuts at: 2659 2659
Size: 5282
BstBI TT'CG_AA
Cuts at: 2094 3643 2094
Size: 1549 3733
BstXI CCAn_nnnn'nTGG
Cuts at: 1385 1794 2046 1385
Size: 409 252 4621
Fragments arranged by size:
4621 409 252
BstYI r'GATC_y
Cuts at: 1 220 882 1017 2064 2307 2774 2797 3132
Size: 219 662 135 1047 243 467 23 335
Cuts at: 3132 3378 3684 3696 3782 4834 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 1047 662 467 449 335 306 246
243 219 135 86 23 12
BtgI C'CryG_G
Cuts at: 599 1511 2193 599
Size: 912 682 3688
Fragments arranged by size:
3688 912 682
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3232 3413 3576 3232
Size: 181 163 4938
Fragments arranged by size:
4938 181 163
BtsI GCAGTG_nn'
Cuts at: 566 1353 2525 566
Size: 787 1172 3323
Fragments arranged by size:
3323 1172 787
DraI TTT'AAA
Cuts at: 932 1590 2281 932
Size: 658 691 3933
Fragments arranged by size:
3933 691 658
DrdI GACnn_nn'nnGTC
Cuts at: 2188 2988 4332 2188
Size: 800 1344 3138
Fragments arranged by size:
3138 1344 800
EaeI y'GGCC_r
Cuts at: 446 1375 2867 3041 3432 3459 4804 446
Size: 929 1492 174 391 27 1345 924
Fragments arranged by size:
1492 1345 929 924 391 174 27
EagI C'GGCC_G
Cuts at: 2867 2867
Size: 5282
EarI CTCTTCn'nnn_
Cuts at: 173 711 3305 3515 173
Size: 538 2594 210 1940
Fragments arranged by size:
2594 1940 538 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 626 3490 4232 4378 5092 626
Size: 2864 742 146 714 816
Fragments arranged by size:
2864 816 742 714 146
EcoICRI GAG'CTC
Cuts at: 2294 2294
Size: 5282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 245 733 973 1466 1730 3107 3539 3892
Size: 126 488 240 493 264 1377 432 353
Cuts at: 3892 4554 119
Size: 662 847
Fragments arranged by size:
1377 847 662 493 488 432 353 264
240 126
EcoO109I rG'GnC_Cy
Cuts at: 489 895 1382 2421 489
Size: 406 487 1039 3350
Fragments arranged by size:
3350 1039 487 406
EcoRI G'AATT_C
Cuts at: 1180 2286 1180
Size: 1106 4176
EcoRV GAT'ATC
Cuts at: 1632 1632
Size: 5282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3301 3301
Size: 5282
FspI TGC'GCA
Cuts at: 3063 3063
Size: 5282
HaeII r_GCGC'y
Cuts at: 2716 2964 4194 4965 2716
Size: 248 1230 771 3033
Fragments arranged by size:
3033 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 1860 1892 3124 3154 3156 3186 379
Size: 32 1449 32 1232 30 2 30 2475
Fragments arranged by size:
2475 1449 1232 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2321 2321
Size: 5282
HindIII A'AGCT_T
Cuts at: 749 749
Size: 5282
KasI G'GCGC_C
Cuts at: 2712 2960 2712
Size: 248 5034
KpnI G_GTAC'C
Cuts at: 1773 2302 1773
Size: 529 4753
MluI A'CGCG_T
Cuts at: 4715 4715
Size: 5282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 871 1506 4041 4225 871
Size: 635 2535 184 1928
Fragments arranged by size:
2535 1928 635 184
MscI TGG'CCA
Cuts at: 3043 3043
Size: 5282
MslI CAynn'nnrTG
Cuts at: 575 1778 1844 3271 3398 575
Size: 1203 66 1427 127 2459
Fragments arranged by size:
2459 1427 1203 127 66
MspA1I CmG'CkG
Cuts at: 1794 2393 2707 3067 3851 4096 1794
Size: 599 314 360 784 245 2980
Fragments arranged by size:
2980 784 599 360 314 245
NaeI GCC'GGC
Cuts at: 3463 3463
Size: 5282
NarI GG'CG_CC
Cuts at: 2713 2961 2713
Size: 248 5034
NcoI C'CATG_G
Cuts at: 599 1511 599
Size: 912 4370
NgoMIV G'CCGG_C
Cuts at: 3461 3461
Size: 5282
NheI G'CTAG_C
Cuts at: 1407 1407
Size: 5282
NspI r_CATG'y
Cuts at: 669 1296 2335 2835 4438 669
Size: 627 1039 500 1603 1513
Fragments arranged by size:
1603 1513 1039 627 500
PciI A'CATG_T
Cuts at: 665 1292 4434 665
Size: 627 3142 1513
Fragments arranged by size:
3142 1513 627
PflMI CCAn_nnn'nTGG
Cuts at: 959 1971 959
Size: 1012 4270
PfoI T'CCnGG_A
Cuts at: 1214 1214
Size: 5282
PmeI GTTT'AAAC
Cuts at: 2281 2281
Size: 5282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1279 1282 1311 1314 3728 3760 1279
Size: 3 29 3 2414 32 2801
Fragments arranged by size:
2801 2414 32 29 3 3
PpuMI rG'GwC_Cy
Cuts at: 489 895 1382 489
Size: 406 487 4389
Fragments arranged by size:
4389 487 406
PsiI TTA'TAA
Cuts at: 1166 1166
Size: 5282
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 793 825 1961 1993 793
Size: 32 1136 32 4082
Fragments arranged by size:
4082 1136 32 32
PstI C_TGCA'G
Cuts at: 2329 3014 2329
Size: 685 4597
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5282
PvuII CAG'CTG
Cuts at: 1794 2707 3067 1794
Size: 913 360 4009
Fragments arranged by size:
4009 913 360
RsrII CG'GwC_CG
Cuts at: 634 3477 634
Size: 2843 2439
SacI G_AGCT'C
Cuts at: 2296 2296
Size: 5282
SalI G'TCGA_C
Cuts at: 2319 2319
Size: 5282
SapI GCTCTTCn'nnn_
Cuts at: 3305 3515 3305
Size: 210 5072
SbfI CC_TGCA'GG
Cuts at: 2329 2329
Size: 5282
ScaI AGT'ACT
Cuts at: 4799 4799
Size: 5282
SfcI C'TryA_G
Cuts at: 33 861 1281 2230 2325 3010 3978 4169 33
Size: 828 420 949 95 685 968 191 1146
Fragments arranged by size:
1146 968 949 828 685 420 191 95
SfoI GGC'GCC
Cuts at: 2714 2962 2714
Size: 248 5034
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
SmaI CCC'GGG
Cuts at: 1468 2304 1468
Size: 836 4446
SmlI C'TyrA_G
Cuts at: 280 532 2437 3352 4066 4328 280
Size: 252 1905 915 714 262 1234
Fragments arranged by size:
1905 1234 915 714 262 252
SnaBI TAC'GTA
Cuts at: 1558 1558
Size: 5282
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5282
SphI G_CATG'C
Cuts at: 2335 2835 2335
Size: 500 4782
StuI AGG'CCT
Cuts at: 443 443
Size: 5282
StyI C'CwwG_G
Cuts at: 253 521 599 1378 1511 1623 2416 2770 253
Size: 268 78 779 133 112 793 354 2765
Fragments arranged by size:
2765 793 779 354 268 133 112 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 2865 2998 3457 3666 580
Size: 2285 133 459 209 2196
Fragments arranged by size:
2285 2196 459 209 133
TatI w'GTAC_w
Cuts at: 800 2074 4797 800
Size: 1274 2723 1285
Fragments arranged by size:
2723 1285 1274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 706 738 931 963 4799 4831 706
Size: 32 193 32 3836 32 1157
Fragments arranged by size:
3836 1157 193 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3079 3079
Size: 5282
XbaI T'CTAG_A
Cuts at: 2313 2313
Size: 5282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 997 2777 997
Size: 1780 3502
XmaI C'CCGG_G
Cuts at: 1466 2302 1466
Size: 836 4446
XmnI GAAnn'nnTTC
Cuts at: 1294 1294
Size: 5282
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BsgI BsmI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PmlI PshAI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI
XhoI ZraI