Restriction map for
pF1KB3481
(Circular) (Six-base) MAPSORT of: pF1KB3481.seq Check: 6922 from: 1 to: 5459
pF1KB3481 5459 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 231 231
Size: 5459
AccI GT'mk_AC
Cuts at: 2497 2497
Size: 5459
Acc65I G'GTAC_C
Cuts at: 2475 2475
Size: 5459
AclI AA'CG_TT
Cuts at: 954 5299 954
Size: 4345 1114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 734 3284 3716 4463 4731 328
Size: 406 2550 432 747 268 1056
Fragments arranged by size:
2550 1056 747 432 406 268
AfeI AGC'GCT
Cuts at: 2443 2443
Size: 5459
AflII C'TTAA_G
Cuts at: 702 702
Size: 5459
AflIII A'CryG_T
Cuts at: 2421 2811 3915 4892 2421
Size: 390 1104 977 2988
Fragments arranged by size:
2988 1104 977 390
AgeI A'CCGG_T
Cuts at: 3847 3847
Size: 5459
AleI CACnn'nnGTG
Cuts at: 262 1477 262
Size: 1215 4244
AlwNI CAG_nnn'CTG
Cuts at: 1746 2266 2764 4331 1746
Size: 520 498 1567 2874
Fragments arranged by size:
2874 1567 520 498
ApaI G_GGCC'C
Cuts at: 797 797
Size: 5459
ApaLI G'TGCA_C
Cuts at: 1103 1435 4229 1103
Size: 332 2794 2333
Fragments arranged by size:
2794 2333 332
ApoI r'AATT_y
Cuts at: 1548 1657 1726 1880 1890 2302 2463 4812 5068
Size: 109 69 154 10 412 161 2349 256
Cuts at: 5068 1548
Size: 1939
Fragments arranged by size:
2349 1939 412 256 161 154 109 69
10
AseI AT'TA_AT
Cuts at: 20 20
Size: 5459
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5459
AvaI C'yCGr_G
Cuts at: 195 806 2479 195
Size: 611 1673 3175
Fragments arranged by size:
3175 1673 611
BamHI G'GATC_C
Cuts at: 2484 2484
Size: 5459
BanI G'GyrC_C
Cuts at: 256 1640 2341 2409 2475 2889 3137 3172 256
Size: 1384 701 68 66 414 248 35 2543
Fragments arranged by size:
2543 1384 701 414 248 68 66 35
BanII G_rGCy'C
Cuts at: 734 797 2473 3503 734
Size: 63 1676 1030 2690
Fragments arranged by size:
2690 1676 1030 63
BbeI G_GCGC'C
Cuts at: 2413 2893 3141 2413
Size: 480 248 4731
Fragments arranged by size:
4731 480 248
BbsI GAAGACnn'nnnn_
Cuts at: 2210 2210
Size: 5459
BbvCI CC'TCA_GC
Cuts at: 1631 1631
Size: 5459
BciVI GTATCCnnnnn_n'
Cuts at: 2106 3357 4118 5459 2106
Size: 1251 761 1341 2106
Fragments arranged by size:
2106 1341 1251 761
BclI T'GATC_A
Cuts at: 1748 2174 2979 1748
Size: 426 805 4228
Fragments arranged by size:
4228 805 426
BglII A'GATC_T
Cuts at: 1271 1982 2974 5011 1271
Size: 711 992 2037 1719
Fragments arranged by size:
2037 1719 992 711
BlpI GC'TnA_GC
Cuts at: 535 2571 535
Size: 2036 3423
Bme1580I G_kGCm'C
Cuts at: 797 1107 1439 1645 3084 3177 4233 797
Size: 310 332 206 1439 93 1056 2023
Fragments arranged by size:
2023 1439 1056 332 310 206 93
BmrI ACTGGGnnnn_n'
Cuts at: 1230 2671 2752 3087 5231 1230
Size: 1441 81 335 2144 1458
Fragments arranged by size:
2144 1458 1441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2409 3826 2409
Size: 1417 4042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 362 566 658 931 988 1870 1986 2295
Size: 53 204 92 273 57 882 116 309
Cuts at: 2295 309
Size: 3473
Fragments arranged by size:
3473 882 309 273 204 116 92 57
53
Bpu10I CC'TnA_GC
Cuts at: 1565 1631 1565
Size: 66 5393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 2635 3514 4006 4304 1381
Size: 1254 879 492 298 2536
Fragments arranged by size:
2536 1254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 113 5163 113
Size: 5050 409
BsaAI yAC'GTr
Cuts at: 2812 2812
Size: 5459
BsaBI GATnn'nnATC
Cuts at: 6 2973 5010 6
Size: 2967 2037 455
Fragments arranged by size:
2967 2037 455
BsaHI Gr'CG_yC
Cuts at: 191 2007 2410 2890 3138 3840 5336 191
Size: 1816 403 480 248 702 1496 314
Fragments arranged by size:
1816 1496 702 480 403 314 248
BsaWI w'CCGG_w
Cuts at: 998 2648 3169 3847 4121 4268 4679 998
Size: 1650 521 678 274 147 411 1778
Fragments arranged by size:
1778 1650 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 307 337 307
Size: 30 5429
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2212 2296 2299 2212
Size: 84 3 5372
Fragments arranged by size:
5372 84 3
BseYI C'CCAG_C
Cuts at: 1197 1344 1497 2296 2413 2884 3642 4219 4715
Size: 147 153 799 117 471 758 577 496
Cuts at: 4715 1197
Size: 1941
Fragments arranged by size:
1941 799 758 577 496 471 153 147
117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 1501
Size: 5459
BsiEI CG_ry'CG
Cuts at: 104 3001 3047 4255 104
Size: 2897 46 1208 1308
Fragments arranged by size:
2897 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 734 1107 1439 1472 2473 3251 3441 4233 734
Size: 373 332 33 1001 778 190 792 1960
Fragments arranged by size:
1960 1001 792 778 373 332 190 33
BsmI GAATG_Cn'
Cuts at: 700 700
Size: 5459
BsmBI CGTCTCn'nnnn_
Cuts at: 1865 1865
Size: 5459
Bsp1286I G_dGCh'C
Cuts at: 734 797 1107 1439 1472 1645 2473 3084 3177
Size: 63 310 332 33 173 828 611 93
Cuts at: 3177 3251 3441 3503 4233 734
Size: 74 190 62 730 1960
Fragments arranged by size:
1960 828 730 611 332 310 190 173
93 74 63 62 33
BspEI T'CCGG_A
Cuts at: 998 4679 998
Size: 3681 1778
BspHI T'CATG_A
Cuts at: 1418 1758 4635 1418
Size: 340 2877 2242
Fragments arranged by size:
2877 2242 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 231 2509 2723 3025 3406 231
Size: 2278 214 302 381 2284
Fragments arranged by size:
2284 2278 381 302 214
BsrBI CCG'CTC
Cuts at: 50 127 2433 3751 3805 5289 5454 50
Size: 77 2306 1318 54 1484 165 55
Fragments arranged by size:
2306 1484 1318 165 77 55 54
BsrDI GCAATG_nn'
Cuts at: 3371 3371
Size: 5459
BsrFI r'CCGG_y
Cuts at: 1277 3457 3638 3847 1277
Size: 2180 181 209 2889
Fragments arranged by size:
2889 2180 209 181
BsrGI T'GTAC_A
Cuts at: 1706 2378 2424 1706
Size: 672 46 4741
Fragments arranged by size:
4741 672 46
BssSI C'ACGA_G
Cuts at: 3730 4088 3730
Size: 358 5101
BstAPI GCAn_nnn'nTGC
Cuts at: 2836 2836
Size: 5459
BstBI TT'CG_AA
Cuts at: 3820 3820
Size: 5459
BstXI CCAn_nnnn'nTGG
Cuts at: 1587 2271 1587
Size: 684 4775
BstYI r'GATC_y
Cuts at: 1 816 1271 1982 2314 2484 2951 2974 3309
Size: 815 455 711 332 170 467 23 335
Cuts at: 3309 3555 4567 4653 4665 5011 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 815 711 467 455 449 346 335
332 246 170 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 209 526 209
Size: 317 5142
BtgI C'CryG_G
Cuts at: 107 842 1992 107
Size: 735 1150 3574
Fragments arranged by size:
3574 1150 735
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1596 2381 3409 3590 3753 1596
Size: 785 1028 181 163 3302
Fragments arranged by size:
3302 1028 785 181 163
BtsI GCAGTG_nn'
Cuts at: 792 1231 2702 792
Size: 439 1471 3549
Fragments arranged by size:
3549 1471 439
DraI TTT'AAA
Cuts at: 1547 2458 1547
Size: 911 4548
DrdI GACnn_nn'nnGTC
Cuts at: 3165 4023 3165
Size: 858 4601
EaeI y'GGCC_r
Cuts at: 351 3044 3218 3609 3636 4981 351
Size: 2693 174 391 27 1345 829
Fragments arranged by size:
2693 1345 829 391 174 27
EagI C'GGCC_G
Cuts at: 3044 3044
Size: 5459
EarI CTCTTCn'nnn_
Cuts at: 167 179 890 1066 2060 2222 2440 3482 3692
Size: 12 711 176 994 162 218 1042 210
Cuts at: 3692 167
Size: 1934
Fragments arranged by size:
1934 1042 994 711 218 210 176 162
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3667 3977 4123 5269 3667
Size: 310 146 1146 3857
Fragments arranged by size:
3857 1146 310 146
EcoICRI GAG'CTC
Cuts at: 732 2471 732
Size: 1739 3720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 328 362 566 658 734 931 988 1870
Size: 19 34 204 92 76 197 57 882
Cuts at: 1870 1986 2295 3284 3716 4463 4731 309
Size: 116 309 989 432 747 268 1037
Fragments arranged by size:
1037 989 882 747 432 309 268 204
197 116 92 76 57 34 19
EcoO109I rG'GnC_Cy
Cuts at: 329 678 794 1364 1413 2598 329
Size: 349 116 570 49 1185 3190
Fragments arranged by size:
3190 1185 570 349 116 49
EcoRI G'AATT_C
Cuts at: 2463 2463
Size: 5459
EcoRV GAT'ATC
Cuts at: 1023 1023
Size: 5459
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 630 3478 630
Size: 2848 2611
FspI TGC'GCA
Cuts at: 3240 3240
Size: 5459
HaeII r_GCGC'y
Cuts at: 2337 2413 2445 2893 3141 4163 5142 2337
Size: 76 32 448 248 1022 979 2654
Fragments arranged by size:
2654 1022 979 448 248 76 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 307 339 3301 3331 3333 3363 307
Size: 32 2962 30 2 30 2403
Fragments arranged by size:
2962 2403 32 30 30 2
HincII GTy'rAC
Cuts at: 1014 2498 1014
Size: 1484 3975
KasI G'GCGC_C
Cuts at: 2409 2889 3137 2409
Size: 480 248 4731
Fragments arranged by size:
4731 480 248
KpnI G_GTAC'C
Cuts at: 2479 2479
Size: 5459
MluI A'CGCG_T
Cuts at: 4892 4892
Size: 5459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4130 4314 4130
Size: 184 5275
MscI TGG'CCA
Cuts at: 353 3220 353
Size: 2867 2592
MslI CAynn'nnrTG
Cuts at: 262 446 653 841 1477 3448 3575 262
Size: 184 207 188 636 1971 127 2146
Fragments arranged by size:
2146 1971 636 207 188 184 127
MspA1I CmG'CkG
Cuts at: 306 484 1048 1210 2570 2884 3244 4257 4502
Size: 178 564 162 1360 314 360 1013 245
Cuts at: 4502 306
Size: 1263
Fragments arranged by size:
1360 1263 1013 564 360 314 245 178
162
NaeI GCC'GGC
Cuts at: 3640 3640
Size: 5459
NarI GG'CG_CC
Cuts at: 2410 2890 3138 2410
Size: 480 248 4731
Fragments arranged by size:
4731 480 248
NcoI C'CATG_G
Cuts at: 107 842 1992 107
Size: 735 1150 3574
Fragments arranged by size:
3574 1150 735
NgoMIV G'CCGG_C
Cuts at: 3638 3638
Size: 5459
NspI r_CATG'y
Cuts at: 1172 2425 2512 3012 3919 1172
Size: 1253 87 500 907 2712
Fragments arranged by size:
2712 1253 907 500 87
PciI A'CATG_T
Cuts at: 2421 3915 2421
Size: 1494 3965
PfoI T'CCnGG_A
Cuts at: 148 674 1424 148
Size: 526 750 4183
Fragments arranged by size:
4183 750 526
PmeI GTTT'AAAC
Cuts at: 2458 2458
Size: 5459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 4598
Size: 32 5427
PpuMI rG'GwC_Cy
Cuts at: 678 1364 678
Size: 686 4773
PshAI GACnn'nnGTC
Cuts at: 547 1516 1857 547
Size: 969 341 4149
Fragments arranged by size:
4149 969 341
PspOMI G'GGCC_C
Cuts at: 793 793
Size: 5459
PstI C_TGCA'G
Cuts at: 1598 2506 3191 1598
Size: 908 685 3866
Fragments arranged by size:
3866 908 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5459
PvuII CAG'CTG
Cuts at: 484 1048 1210 2884 3244 484
Size: 564 162 1674 360 2699
Fragments arranged by size:
2699 1674 564 360 162
RsrII CG'GwC_CG
Cuts at: 129 3654 129
Size: 3525 1934
SacI G_AGCT'C
Cuts at: 734 2473 734
Size: 1739 3720
SalI G'TCGA_C
Cuts at: 2496 2496
Size: 5459
SapI GCTCTTCn'nnn_
Cuts at: 1066 2222 2440 3482 3692 1066
Size: 1156 218 1042 210 2833
Fragments arranged by size:
2833 1156 1042 218 210
SbfI CC_TGCA'GG
Cuts at: 2506 2506
Size: 5459
ScaI AGT'ACT
Cuts at: 4976 4976
Size: 5459
SfcI C'TryA_G
Cuts at: 33 288 1594 2502 3187 4180 4371 33
Size: 255 1306 908 685 993 191 1121
Fragments arranged by size:
1306 1121 993 908 685 255 191
SfoI GGC'GCC
Cuts at: 2411 2891 3139 2411
Size: 480 248 4731
Fragments arranged by size:
4731 480 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5459
SmaI CCC'GGG
Cuts at: 197 2481 197
Size: 2284 3175
SmlI C'TyrA_G
Cuts at: 702 1396 2614 3529 4021 4283 702
Size: 694 1218 915 492 262 1878
Fragments arranged by size:
1878 1218 915 694 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5459
SphI G_CATG'C
Cuts at: 1172 2512 3012 1172
Size: 1340 500 3619
Fragments arranged by size:
3619 1340 500
StyI C'CwwG_G
Cuts at: 107 281 842 1295 1931 1992 2593 2947 107
Size: 174 561 453 636 61 601 354 2619
Fragments arranged by size:
2619 636 601 561 453 354 174 61
SwaI ATTT'AAAT
Cuts at: 1547 1547
Size: 5459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 137 327 3042 3175 3634 3843 137
Size: 190 2715 133 459 209 1753
Fragments arranged by size:
2715 1753 459 209 190 133
TatI w'GTAC_w
Cuts at: 452 583 1706 2069 2378 2424 4974 452
Size: 131 1123 363 309 46 2550 937
Fragments arranged by size:
2550 1123 937 363 309 131 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4976 5008 4976
Size: 32 5427
Tth111I GACn'n_nGTC
Cuts at: 909 3256 909
Size: 2347 3112
XbaI T'CTAG_A
Cuts at: 2490 2490
Size: 5459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2954 2954
Size: 5459
XmaI C'CCGG_G
Cuts at: 195 2479 195
Size: 2284 3175
XmnI GAAnn'nnTTC
Cuts at: 218 218
Size: 5459
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AloI AscI AvrII BaeI BcgI
BfrBI BglI BmgBI BmtI BsiWI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI XhoI ZraI