Restriction map for
pF1KB3482
(Circular) (Six-base) MAPSORT of: pF1KB3482.seq Check: 5135 from: 1 to: 5312
pF1KB3482 5312 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 447 2167 447
Size: 1720 3592
AccI GT'mk_AC
Cuts at: 2350 2350
Size: 5312
Acc65I G'GTAC_C
Cuts at: 2328 2328
Size: 5312
AclI AA'CG_TT
Cuts at: 979 5152 979
Size: 4173 1139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 980 1037 1045 1346 1522 1651 1664 3137
Size: 489 57 8 301 176 129 13 1473
Cuts at: 3137 3569 3922 4584 491
Size: 432 353 662 1219
Fragments arranged by size:
1473 1219 662 489 432 353 301 176
129 57 13 8
AflIII A'CryG_T
Cuts at: 2664 4464 4745 2664
Size: 1800 281 3231
Fragments arranged by size:
3231 1800 281
AleI CACnn'nnGTG
Cuts at: 1897 1897
Size: 5312
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 268 300 268
Size: 32 5280
AlwNI CAG_nnn'CTG
Cuts at: 1500 2617 4055 1500
Size: 1117 1438 2757
Fragments arranged by size:
2757 1438 1117
ApaLI G'TGCA_C
Cuts at: 4150 4150
Size: 5312
ApoI r'AATT_y
Cuts at: 254 320 874 1124 2053 2316 4665 4921 254
Size: 66 554 250 929 263 2349 256 645
Fragments arranged by size:
2349 929 645 554 263 256 250 66
AseI AT'TA_AT
Cuts at: 20 20
Size: 5312
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5312
AvaI C'yCGr_G
Cuts at: 1299 2332 1299
Size: 1033 4279
BamHI G'GATC_C
Cuts at: 2337 2337
Size: 5312
BanI G'GyrC_C
Cuts at: 2172 2328 2742 2990 3025 2172
Size: 156 414 248 35 4459
Fragments arranged by size:
4459 414 248 156 35
BanII G_rGCy'C
Cuts at: 498 1730 2326 3356 498
Size: 1232 596 1030 2454
Fragments arranged by size:
2454 1232 1030 596
BbeI G_GCGC'C
Cuts at: 2746 2994 2746
Size: 248 5064
BbsI GAAGACnn'nnnn_
Cuts at: 353 1114 1463 1639 1667 1773 353
Size: 761 349 176 28 106 3892
Fragments arranged by size:
3892 761 349 176 106 28
BciVI GTATCCnnnnn_n'
Cuts at: 3210 4266 5312 3210
Size: 1056 1046 3210
Fragments arranged by size:
3210 1056 1046
BclI T'GATC_A
Cuts at: 2832 2832
Size: 5312
BfrBI ATG'CAT
Cuts at: 1266 1266
Size: 5312
BglI GCCn_nnn'nGGC
Cuts at: 1762 1762
Size: 5312
BglII A'GATC_T
Cuts at: 2203 2827 4864 2203
Size: 624 2037 2651
Fragments arranged by size:
2651 2037 624
BlpI GC'TnA_GC
Cuts at: 2424 2424
Size: 5312
Bme1580I G_kGCm'C
Cuts at: 1671 2937 3030 4154 1671
Size: 1266 93 1124 2829
Fragments arranged by size:
2829 1266 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 2524 2605 2940 5084 724
Size: 1800 81 335 2144 952
Fragments arranged by size:
2144 1800 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1219 3679 1219
Size: 2460 2852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 1189 1897 512
Size: 677 708 3927
Fragments arranged by size:
3927 708 677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2488 3367 4081 4379 2488
Size: 879 714 298 3421
Fragments arranged by size:
3421 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 769 5016 769
Size: 4247 1065
BsaAI yAC'GTr
Cuts at: 2665 2665
Size: 5312
BsaBI GATnn'nnATC
Cuts at: 6 2826 4863 6
Size: 2820 2037 455
Fragments arranged by size:
2820 2037 455
BsaHI Gr'CG_yC
Cuts at: 2743 2991 3693 5189 2743
Size: 248 702 1496 2866
Fragments arranged by size:
2866 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2501 3022 3700 4111 4258 4532 2501
Size: 521 678 411 147 274 3281
Fragments arranged by size:
3281 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 555 585 1450 1480 555
Size: 30 865 30 4387
Fragments arranged by size:
4387 865 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1394 1562 1394
Size: 168 5144
BseYI C'CCAG_C
Cuts at: 1548 2737 3495 4160 4568 1548
Size: 1189 758 665 408 2292
Fragments arranged by size:
2292 1189 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 5312
BsiEI CG_ry'CG
Cuts at: 104 2854 2900 4130 104
Size: 2750 46 1230 1286
Fragments arranged by size:
2750 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 498 2326 3104 3294 4154 498
Size: 1828 778 190 860 1656
Fragments arranged by size:
1828 1656 860 778 190
BsmI GAATG_Cn'
Cuts at: 1917 1917
Size: 5312
Bsp1286I G_dGCh'C
Cuts at: 498 1671 1730 2326 2937 3030 3104 3294 3356
Size: 1173 59 596 611 93 74 190 62
Cuts at: 3356 4154 498
Size: 798 1656
Fragments arranged by size:
1656 1173 798 611 596 190 93 74
62 59
BspHI T'CATG_A
Cuts at: 1484 1532 3744 1484
Size: 48 2212 3052
Fragments arranged by size:
3052 2212 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 447 2167 2362 2576 2878 3259 447
Size: 1720 195 214 302 381 2500
Fragments arranged by size:
2500 1720 381 302 214 195
BsrBI CCG'CTC
Cuts at: 50 3604 3658 5142 5307 50
Size: 3554 54 1484 165 55
Fragments arranged by size:
3554 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1872 3224 1838
Size: 34 1352 3926
Fragments arranged by size:
3926 1352 34
BsrFI r'CCGG_y
Cuts at: 589 3310 3491 589
Size: 2721 181 2410
Fragments arranged by size:
2721 2410 181
BssSI C'ACGA_G
Cuts at: 3583 4291 3583
Size: 708 4604
BstAPI GCAn_nnn'nTGC
Cuts at: 2689 2689
Size: 5312
BstBI TT'CG_AA
Cuts at: 326 3673 326
Size: 3347 1965
BstXI CCAn_nnnn'nTGG
Cuts at: 606 606
Size: 5312
BstYI r'GATC_y
Cuts at: 1 1931 2203 2337 2804 2827 3162 3408 3714
Size: 1930 272 134 467 23 335 246 306
Cuts at: 3714 3726 3812 4864 1
Size: 12 86 1052 449
Fragments arranged by size:
1930 1052 467 449 335 306 272 246
134 86 23 12
BtgI C'CryG_G
Cuts at: 605 605
Size: 5312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3262 3443 3606 3262
Size: 181 163 4968
Fragments arranged by size:
4968 181 163
BtsI GCAGTG_nn'
Cuts at: 2555 2555
Size: 5312
DraI TTT'AAA
Cuts at: 218 230 854 2311 218
Size: 12 624 1457 3219
Fragments arranged by size:
3219 1457 624 12
DrdI GACnn_nn'nnGTC
Cuts at: 636 3018 4362 636
Size: 2382 1344 1586
Fragments arranged by size:
2382 1586 1344
EaeI y'GGCC_r
Cuts at: 683 2897 3071 3462 3489 4834 683
Size: 2214 174 391 27 1345 1161
Fragments arranged by size:
2214 1345 1161 391 174 27
EagI C'GGCC_G
Cuts at: 2897 2897
Size: 5312
EarI CTCTTCn'nnn_
Cuts at: 236 3335 3545 236
Size: 3099 210 2003
Fragments arranged by size:
3099 2003 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3520 4262 4408 5122 3520
Size: 742 146 714 3710
Fragments arranged by size:
3710 742 714 146
EcoICRI GAG'CTC
Cuts at: 496 2324 496
Size: 1828 3484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 512 980 1037 1045 1189 1346 1522 1651
Size: 21 468 57 8 144 157 176 129
Cuts at: 1651 1664 1897 3137 3569 3922 4584 491
Size: 13 233 1240 432 353 662 1219
Fragments arranged by size:
1240 1219 662 468 432 353 233 176
157 144 129 57 21 13 8
EcoO109I rG'GnC_Cy
Cuts at: 2451 2451
Size: 5312
EcoRI G'AATT_C
Cuts at: 2053 2316 2053
Size: 263 5049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3331 3331
Size: 5312
FspI TGC'GCA
Cuts at: 3093 3093
Size: 5312
HaeII r_GCGC'y
Cuts at: 2746 2994 4224 4995 2746
Size: 248 1230 771 3063
Fragments arranged by size:
3063 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 149 181 555 587 1450 1482 2188 2220 3154
Size: 32 374 32 863 32 706 32 934
Cuts at: 3154 3184 3186 3216 149
Size: 30 2 30 2245
Fragments arranged by size:
2245 934 863 706 374 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2351 2351
Size: 5312
HindIII A'AGCT_T
Cuts at: 1049 1049
Size: 5312
KasI G'GCGC_C
Cuts at: 2742 2990 2742
Size: 248 5064
KpnI G_GTAC'C
Cuts at: 2332 2332
Size: 5312
MluI A'CGCG_T
Cuts at: 4745 4745
Size: 5312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4071 4255 4071
Size: 184 5128
MscI TGG'CCA
Cuts at: 685 3073 685
Size: 2388 2924
MslI CAynn'nnrTG
Cuts at: 604 1489 1897 3301 3428 604
Size: 885 408 1404 127 2488
Fragments arranged by size:
2488 1404 885 408 127
MspA1I CmG'CkG
Cuts at: 1552 2423 2737 3097 3881 4126 1552
Size: 871 314 360 784 245 2738
Fragments arranged by size:
2738 871 784 360 314 245
NaeI GCC'GGC
Cuts at: 591 3493 591
Size: 2902 2410
NarI GG'CG_CC
Cuts at: 2743 2991 2743
Size: 248 5064
NcoI C'CATG_G
Cuts at: 605 605
Size: 5312
NgoMIV G'CCGG_C
Cuts at: 589 3491 589
Size: 2902 2410
NsiI A_TGCA'T
Cuts at: 1268 1268
Size: 5312
NspI r_CATG'y
Cuts at: 597 2365 2865 4468 597
Size: 1768 500 1603 1441
Fragments arranged by size:
1768 1603 1441 500
PciI A'CATG_T
Cuts at: 4464 4464
Size: 5312
PfoI T'CCnGG_A
Cuts at: 489 1521 1874 489
Size: 1032 353 3927
Fragments arranged by size:
3927 1032 353
PmeI GTTT'AAAC
Cuts at: 2311 2311
Size: 5312
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 5280
PstI C_TGCA'G
Cuts at: 585 2359 3044 585
Size: 1774 685 2853
Fragments arranged by size:
2853 1774 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5312
PvuII CAG'CTG
Cuts at: 1552 2737 3097 1552
Size: 1185 360 3767
Fragments arranged by size:
3767 1185 360
RsrII CG'GwC_CG
Cuts at: 3507 3507
Size: 5312
SacI G_AGCT'C
Cuts at: 498 2326 498
Size: 1828 3484
SalI G'TCGA_C
Cuts at: 2349 2349
Size: 5312
SapI GCTCTTCn'nnn_
Cuts at: 3335 3545 3335
Size: 210 5102
SbfI CC_TGCA'GG
Cuts at: 585 2359 585
Size: 1774 3538
ScaI AGT'ACT
Cuts at: 4829 4829
Size: 5312
SfcI C'TryA_G
Cuts at: 33 581 812 2355 3040 4008 4199 33
Size: 548 231 1543 685 968 191 1146
Fragments arranged by size:
1543 1146 968 685 548 231 191
SfoI GGC'GCC
Cuts at: 2744 2992 2744
Size: 248 5064
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5312
SmaI CCC'GGG
Cuts at: 2334 2334
Size: 5312
SmlI C'TyrA_G
Cuts at: 2467 3382 4096 4358 2467
Size: 915 714 262 3421
Fragments arranged by size:
3421 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5312
SphI G_CATG'C
Cuts at: 2365 2865 2365
Size: 500 4812
SspI AAT'ATT
Cuts at: 1062 1803 1062
Size: 741 4571
StyI C'CwwG_G
Cuts at: 605 2446 2800 605
Size: 1841 354 3117
Fragments arranged by size:
3117 1841 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 663 1934 2895 3028 3487 3696 663
Size: 1271 961 133 459 209 2279
Fragments arranged by size:
2279 1271 961 459 209 133
TatI w'GTAC_w
Cuts at: 751 4827 751
Size: 4076 1236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 821 853 1994 2026 4829 4861 821
Size: 32 1141 32 2803 32 1272
Fragments arranged by size:
2803 1272 1141 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1169 3109 1169
Size: 1940 3372
XbaI T'CTAG_A
Cuts at: 2343 2343
Size: 5312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2807 2807
Size: 5312
XmaI C'CCGG_G
Cuts at: 2332 2332
Size: 5312
XmnI GAAnn'nnTTC
Cuts at: 1480 1480
Size: 5312
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI