Restriction map for
pF1KB3483
(Circular) (Six-base) MAPSORT of: pF1KB3483.seq Check: 7255 from: 1 to: 3872
pF1KB3483 3872 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 910 910
Size: 3872
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1697 2129 2876 3144 1697
Size: 432 747 268 2425
Fragments arranged by size:
2425 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 460 1177 2744 460
Size: 717 1567 1588
Fragments arranged by size:
1588 1567 717
ApaLI G'TGCA_C
Cuts at: 634 2642 634
Size: 2008 1864
ApoI r'AATT_y
Cuts at: 876 3225 3481 876
Size: 2349 256 1267
Fragments arranged by size:
2349 1267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 222 892 222
Size: 670 3202
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 388 888 1302 1550 1585 388
Size: 500 414 248 35 2675
Fragments arranged by size:
2675 500 414 248 35
BanII G_rGCy'C
Cuts at: 886 1916 886
Size: 1030 2842
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 1392 1392
Size: 3872
BglI GCCn_nnn'nGGC
Cuts at: 242 485 242
Size: 243 3629
BglII A'GATC_T
Cuts at: 1387 3424 1387
Size: 2037 1835
BlpI GC'TnA_GC
Cuts at: 984 984
Size: 3872
Bme1580I G_kGCm'C
Cuts at: 638 1497 1590 2646 638
Size: 859 93 1056 1864
Fragments arranged by size:
1864 1056 859 93
BmrI ACTGGGnnnn_n'
Cuts at: 215 750 1084 1165 1500 3644 215
Size: 535 334 81 335 2144 443
Fragments arranged by size:
2144 535 443 335 334 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 788 2239 788
Size: 1451 2421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 260 212
Size: 48 3824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 700 3576 700
Size: 2876 996
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 748 1386 3423 6
Size: 742 638 2037 455
Fragments arranged by size:
2037 742 638 455
BsaHI Gr'CG_yC
Cuts at: 1303 1551 2253 3749 1303
Size: 248 702 1496 1426
Fragments arranged by size:
1496 1426 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1582 2260 2534 2681 3092 1061
Size: 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 804 804
Size: 3872
BseYI C'CCAG_C
Cuts at: 275 299 323 347 371 460 495 1297 2055
Size: 24 24 24 24 89 35 802 758
Cuts at: 2055 2632 3128 275
Size: 577 496 1019
Fragments arranged by size:
1019 802 758 577 496 89 35 24
24 24 24
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 638 886 1664 1854 2646 638
Size: 248 778 190 792 1864
Fragments arranged by size:
1864 792 778 248 190
Bsp1286I G_dGCh'C
Cuts at: 638 886 1497 1590 1664 1854 1916 2646 638
Size: 248 611 93 74 190 62 730 1864
Fragments arranged by size:
1864 730 611 248 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 3048 3048
Size: 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 922 1136 1438 1819 922
Size: 214 302 381 2975
Fragments arranged by size:
2975 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2164 2218 3702 3867 50
Size: 2114 54 1484 165 55
Fragments arranged by size:
2114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1784 1784
Size: 3872
BsrFI r'CCGG_y
Cuts at: 1870 2051 2260 1870
Size: 181 209 3482
Fragments arranged by size:
3482 209 181
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 1249 1249
Size: 3872
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstEII G'GTnAC_C
Cuts at: 672 672
Size: 3872
BstXI CCAn_nnnn'nTGG
Cuts at: 343 343
Size: 3872
BstYI r'GATC_y
Cuts at: 1 897 1364 1387 1722 1968 2980 3066 3078
Size: 896 467 23 335 246 1012 86 12
Cuts at: 3078 3424 1
Size: 346 449
Fragments arranged by size:
1012 896 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 260 260
Size: 3872
BtgI C'CryG_G
Cuts at: 107 336 602 107
Size: 229 266 3377
Fragments arranged by size:
3377 266 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 473 540 1115 473
Size: 67 575 3230
Fragments arranged by size:
3230 575 67
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 146 1457 1631 2022 2049 3394 146
Size: 1311 174 391 27 1345 624
Fragments arranged by size:
1345 1311 624 391 174 27
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 814 1895 2105 814
Size: 1081 210 2581
Fragments arranged by size:
2581 1081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2080 2390 2536 3682 2080
Size: 310 146 1146 2270
Fragments arranged by size:
2270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 884 884
Size: 3872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 260 1697 2129 2876 3144 212
Size: 48 1437 432 747 268 940
Fragments arranged by size:
1437 940 747 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 477 1011 477
Size: 534 3338
EcoRI G'AATT_C
Cuts at: 876 876
Size: 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 183 1306 1554 2576 3555 183
Size: 1123 248 1022 979 500
Fragments arranged by size:
1123 1022 979 500 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 754 786 1714 1744 1746 1776 754
Size: 32 928 30 2 30 2850
Fragments arranged by size:
2850 928 32 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2727 2543
Size: 184 3688
MscI TGG'CCA
Cuts at: 148 1633 148
Size: 1485 2387
MslI CAynn'nnrTG
Cuts at: 293 317 341 365 1861 1988 293
Size: 24 24 24 1496 127 2177
Fragments arranged by size:
2177 1496 127 24 24 24
MspA1I CmG'CkG
Cuts at: 460 983 1297 1657 2670 2915 460
Size: 523 314 360 1013 245 1417
Fragments arranged by size:
1417 1013 523 360 314 245
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 3872
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NcoI C'CATG_G
Cuts at: 107 336 602 107
Size: 229 266 3377
Fragments arranged by size:
3377 266 229
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 3872
NspI r_CATG'y
Cuts at: 495 570 925 1425 2332 495
Size: 75 355 500 907 2035
Fragments arranged by size:
2035 907 500 355 75
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PstI C_TGCA'G
Cuts at: 456 919 1604 456
Size: 463 685 2724
Fragments arranged by size:
2724 685 463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 460 1297 1657 460
Size: 837 360 2675
Fragments arranged by size:
2675 837 360
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 886 886
Size: 3872
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 3389 3389
Size: 3872
SfcI C'TryA_G
Cuts at: 33 452 594 915 1600 2593 2784 33
Size: 419 142 321 685 993 191 1121
Fragments arranged by size:
1121 993 685 419 321 191 142
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 224 894 224
Size: 670 3202
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 925 1425 925
Size: 500 3372
StuI AGG'CCT
Cuts at: 778 778
Size: 3872
StyI C'CwwG_G
Cuts at: 107 117 336 480 602 686 1006 1360 107
Size: 10 219 144 122 84 320 354 2619
Fragments arranged by size:
2619 354 320 219 144 122 84 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 724 769 1455 1588 2047 2256 406
Size: 318 45 686 133 459 209 2022
Fragments arranged by size:
2022 686 459 318 209 133 45
TatI w'GTAC_w
Cuts at: 590 611 3387 590
Size: 21 2776 1075
Fragments arranged by size:
2776 1075 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 812 844 3389 3421 812
Size: 32 2545 32 1263
Fragments arranged by size:
2545 1263 32 32
Tth111I GACn'n_nGTC
Cuts at: 643 1669 643
Size: 1026 2846
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1367 1367
Size: 3872
XmaI C'CCGG_G
Cuts at: 222 892 222
Size: 670 3202
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI