Restriction map for
pF1KB3486
(Circular) (Six-base) MAPSORT of: pF1KB3486.seq Check: 1683 from: 1 to: 4217
pF1KB3486 4217 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 729 729
Size: 4217
AatII G_ACGT'C
Cuts at: 1103 1103
Size: 4217
AccI GT'mk_AC
Cuts at: 1255 1255
Size: 4217
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 580 601 2042 2474 3221 3489 145
Size: 435 21 1441 432 747 268 873
Fragments arranged by size:
1441 873 747 435 432 268 21
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AhdI GACnn_n'nnGTC
Cuts at: 1158 1158
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 543 666 804 1522 3089 543
Size: 123 138 718 1567 1671
Fragments arranged by size:
1671 1567 718 138 123
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 1221 3570 3826 1221
Size: 2349 256 1612
Fragments arranged by size:
2349 1612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 884 1063 1237 884
Size: 179 174 3864
Fragments arranged by size:
3864 179 174
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 1233 1647 1895 1930 1233
Size: 414 248 35 3520
Fragments arranged by size:
3520 414 248 35
BanII G_rGCy'C
Cuts at: 893 1064 1231 2261 893
Size: 171 167 1030 2849
Fragments arranged by size:
2849 1030 171 167
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 947 981 947
Size: 34 4183
BciVI GTATCCnnnnn_n'
Cuts at: 2115 2876 4217 2115
Size: 761 1341 2115
Fragments arranged by size:
2115 1341 761
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglI GCCn_nnn'nGGC
Cuts at: 301 1163 301
Size: 862 3355
BglII A'GATC_T
Cuts at: 1732 3769 1732
Size: 2037 2180
BlpI GC'TnA_GC
Cuts at: 305 1177 1329 305
Size: 872 152 3193
Fragments arranged by size:
3193 872 152
Bme1580I G_kGCm'C
Cuts at: 1202 1842 1935 2991 1202
Size: 640 93 1056 2428
Fragments arranged by size:
2428 1056 640 93
BmrI ACTGGGnnnn_n'
Cuts at: 1429 1510 1845 3989 1429
Size: 81 335 2144 1657
Fragments arranged by size:
2144 1657 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 610 688 742 520
Size: 90 78 54 3995
Fragments arranged by size:
3995 90 78 54
Bpu10I CC'TnA_GC
Cuts at: 860 911 860
Size: 51 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1393 2272 2764 3062 811
Size: 582 879 492 298 1966
Fragments arranged by size:
1966 879 582 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3921 3921
Size: 4217
BsaAI yAC'GTr
Cuts at: 1185 1570 1185
Size: 385 3832
BsaBI GATnn'nnATC
Cuts at: 6 1731 3768 6
Size: 1725 2037 455
Fragments arranged by size:
2037 1725 455
BsaHI Gr'CG_yC
Cuts at: 1100 1648 1896 2598 4094 1100
Size: 548 248 702 1496 1223
Fragments arranged by size:
1496 1223 702 548 248
BsaWI w'CCGG_w
Cuts at: 995 1406 1927 2605 2879 3026 3437 995
Size: 411 521 678 274 147 411 1775
Fragments arranged by size:
1775 678 521 411 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 664 694 664
Size: 30 4187
BseYI C'CCAG_C
Cuts at: 291 417 1080 1642 2400 2977 3473 291
Size: 126 663 562 758 577 496 1035
Fragments arranged by size:
1035 758 663 577 562 496 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 921 817
Size: 104 4113
BsiEI CG_ry'CG
Cuts at: 104 1759 1805 3013 104
Size: 1655 46 1208 1308
Fragments arranged by size:
1655 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1231 2009 2199 2991 1231
Size: 778 190 792 2457
Fragments arranged by size:
2457 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 893 1064 1202 1231 1842 1935 2009 2199 2261
Size: 171 138 29 611 93 74 190 62
Cuts at: 2261 2991 893
Size: 730 2119
Fragments arranged by size:
2119 730 611 190 171 138 93 74
62 29
BspEI T'CCGG_A
Cuts at: 995 3437 995
Size: 2442 1775
BspHI T'CATG_A
Cuts at: 3393 3393
Size: 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 729 1200 1267 1481 1783 2164 287
Size: 442 471 67 214 302 381 2340
Fragments arranged by size:
2340 471 442 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1176 2509 2563 4047 4212 50
Size: 1126 1333 54 1484 165 55
Fragments arranged by size:
1484 1333 1126 165 55 54
BsrDI GCAATG_nn'
Cuts at: 445 2129 445
Size: 1684 2533
BsrFI r'CCGG_y
Cuts at: 457 785 2215 2396 2605 457
Size: 328 1430 181 209 2069
Fragments arranged by size:
2069 1430 328 209 181
BssSI C'ACGA_G
Cuts at: 2488 2846 2488
Size: 358 3859
BstAPI GCAn_nnn'nTGC
Cuts at: 795 1594 795
Size: 799 3418
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 114 693 114
Size: 579 3638
BstYI r'GATC_y
Cuts at: 1 335 566 638 800 1242 1709 1732 2067
Size: 334 231 72 162 442 467 23 335
Cuts at: 2067 2313 3325 3411 3423 3769 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 442 346 335 334 246
231 162 86 72 23 12
BtgI C'CryG_G
Cuts at: 405 405
Size: 4217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 1460 1460
Size: 4217
ClaI AT'CG_AT
Cuts at: 1124 1124
Size: 4217
DraI TTT'AAA
Cuts at: 1216 1216
Size: 4217
DrdI GACnn_nn'nnGTC
Cuts at: 508 1923 2781 508
Size: 1415 858 1944
Fragments arranged by size:
1944 1415 858
EaeI y'GGCC_r
Cuts at: 260 1155 1802 1976 2367 2394 3739 260
Size: 895 647 174 391 27 1345 738
Fragments arranged by size:
1345 895 738 647 391 174 27
EagI C'GGCC_G
Cuts at: 1802 1802
Size: 4217
EarI CTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 832 2425 2735 2881 4027 391
Size: 441 1593 310 146 1146 581
Fragments arranged by size:
1593 1146 581 441 310 146
EcoICRI GAG'CTC
Cuts at: 1229 1229
Size: 4217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 520 580 601 610 688 742 2042 2474
Size: 375 60 21 9 78 54 1300 432
Cuts at: 2474 3221 3489 145
Size: 747 268 873
Fragments arranged by size:
1300 873 747 432 375 268 78 60
54 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 481 1207 481
Size: 726 3491
EcoO109I rG'GnC_Cy
Cuts at: 1356 1356
Size: 4217
EcoRI G'AATT_C
Cuts at: 1221 1221
Size: 4217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 2236 537
Size: 1699 2518
FspI TGC'GCA
Cuts at: 1998 1998
Size: 4217
HaeII r_GCGC'y
Cuts at: 477 667 1048 1651 1899 2921 3900 477
Size: 190 381 603 248 1022 979 794
Fragments arranged by size:
1022 979 794 603 381 248 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1144 1176 2059 2089 2091 2121 1144
Size: 32 883 30 2 30 3240
Fragments arranged by size:
3240 883 32 30 30 2
HincII GTy'rAC
Cuts at: 1256 1256
Size: 4217
HindIII A'AGCT_T
Cuts at: 845 845
Size: 4217
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 150 353 455 944 1007 2888 3072 150
Size: 203 102 489 63 1881 184 1295
Fragments arranged by size:
1881 1295 489 203 184 102 63
MscI TGG'CCA
Cuts at: 262 1978 262
Size: 1716 2501
MslI CAynn'nnrTG
Cuts at: 112 2206 2333 112
Size: 2094 127 1996
Fragments arranged by size:
2094 1996 127
MspA1I CmG'CkG
Cuts at: 153 774 1328 1642 2002 3015 3260 153
Size: 621 554 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 621 554 360 314 245
NaeI GCC'GGC
Cuts at: 459 2398 459
Size: 1939 2278
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NgoMIV G'CCGG_C
Cuts at: 457 2396 457
Size: 1939 2278
NspI r_CATG'y
Cuts at: 711 1017 1149 1270 1770 2677 711
Size: 306 132 121 500 907 2251
Fragments arranged by size:
2251 907 500 306 132 121
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PflMI CCAn_nnn'nTGG
Cuts at: 666 666
Size: 4217
PfoI T'CCnGG_A
Cuts at: 719 803 719
Size: 84 4133
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 346 378 346
Size: 32 4185
PstI C_TGCA'G
Cuts at: 284 1264 1949 284
Size: 980 685 2552
Fragments arranged by size:
2552 980 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 153 774 1642 2002 153
Size: 621 868 360 2368
Fragments arranged by size:
2368 868 621 360
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 1231 1231
Size: 4217
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 3734 3734
Size: 4217
SfcI C'TryA_G
Cuts at: 33 280 1260 1945 2938 3129 33
Size: 247 980 685 993 191 1121
Fragments arranged by size:
1121 993 980 685 247 191
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 886 1239 886
Size: 353 3864
SmlI C'TyrA_G
Cuts at: 826 1372 2287 2779 3041 826
Size: 546 915 492 262 2002
Fragments arranged by size:
2002 915 546 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 711 1017 1149 1270 1770 711
Size: 306 132 121 500 3158
Fragments arranged by size:
3158 500 306 132 121
StyI C'CwwG_G
Cuts at: 686 900 1351 1705 686
Size: 214 451 354 3198
Fragments arranged by size:
3198 451 354 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1800 1933 2392 2601 1800
Size: 133 459 209 3416
Fragments arranged by size:
3416 459 209 133
TatI w'GTAC_w
Cuts at: 3732 3732
Size: 4217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4185
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 474 1712 474
Size: 1238 2979
XmaI C'CCGG_G
Cuts at: 884 1237 884
Size: 353 3864
XmnI GAAnn'nnTTC
Cuts at: 133 133
Size: 4217
ZraI GAC'GTC
Cuts at: 1101 1101
Size: 4217
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI