Restriction map for
pF1KB3490
(Circular) (Six-base) MAPSORT of: pF1KB3490.seq Check: 2638 from: 1 to: 4124
pF1KB3490 4124 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 826 826
Size: 4124
AccI GT'mk_AC
Cuts at: 812 1162 812
Size: 350 3774
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 934 968 1949 2381 3128 3396 368
Size: 566 34 981 432 747 268 1096
Fragments arranged by size:
1096 981 747 566 432 268 34
AflIII A'CryG_T
Cuts at: 669 1476 2580 3557 669
Size: 807 1104 977 1236
Fragments arranged by size:
1236 1104 977 807
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 198 560 1429 2996 198
Size: 362 869 1567 1326
Fragments arranged by size:
1567 1326 869 362
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 352 538 1128 3477 3733 352
Size: 186 590 2349 256 743
Fragments arranged by size:
2349 743 590 256 186
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 1144 1144
Size: 4124
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 940 973 940
Size: 33 4091
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 827 1042 1140 1554 1802 1837 827
Size: 215 98 414 248 35 3114
Fragments arranged by size:
3114 414 248 215 98 35
BanII G_rGCy'C
Cuts at: 756 1069 1138 2168 756
Size: 313 69 1030 2712
Fragments arranged by size:
2712 1030 313 69
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 263 263
Size: 4124
BbvCI CC'TCA_GC
Cuts at: 117 117
Size: 4124
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 1063 1236 1063
Size: 173 3951
Bme1580I G_kGCm'C
Cuts at: 830 1749 1842 2898 830
Size: 919 93 1056 2056
Fragments arranged by size:
2056 1056 919 93
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1417 1752 3896 1336
Size: 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 81
BmtI G_CTAG'C
Cuts at: 437 437
Size: 4124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
Bpu10I CC'TnA_GC
Cuts at: 117 424 117
Size: 307 3817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 540 820 1300 2179 2671 2969 109
Size: 431 280 480 879 492 298 1264
Fragments arranged by size:
1264 879 492 480 431 298 280
BsaI GGTCTCn'nnnn_
Cuts at: 106 1091 3828 106
Size: 985 2737 402
Fragments arranged by size:
2737 985 402
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 491 506 1555 1803 2505 4001 491
Size: 15 1049 248 702 1496 614
Fragments arranged by size:
1496 1049 702 614 248 15
BsaWI w'CCGG_w
Cuts at: 1313 1834 2512 2786 2933 3344 1313
Size: 521 678 274 147 411 2093
Fragments arranged by size:
2093 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 817 817
Size: 4124
BseYI C'CCAG_C
Cuts at: 1549 2307 2884 3380 1549
Size: 758 577 496 2293
Fragments arranged by size:
2293 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 597 804 597
Size: 207 3917
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 775 1069 1138 1916 2106 2898 775
Size: 294 69 778 190 792 2001
Fragments arranged by size:
2001 792 778 294 190 69
BsmI GAATG_Cn'
Cuts at: 384 519 384
Size: 135 3989
Bsp1286I G_dGCh'C
Cuts at: 756 775 830 1069 1138 1749 1842 1916 2106
Size: 19 55 239 69 611 93 74 190
Cuts at: 2106 2168 2898 756
Size: 62 730 1982
Fragments arranged by size:
1982 730 611 239 190 93 74 69
62 55 19
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 173 826 1174 1388 1690 2071 173
Size: 653 348 214 302 381 2226
Fragments arranged by size:
2226 653 381 348 302 214
BsrBI CCG'CTC
Cuts at: 50 834 2416 2470 3954 4119 50
Size: 784 1582 54 1484 165 55
Fragments arranged by size:
1582 1484 784 165 55 54
BsrDI GCAATG_nn'
Cuts at: 845 1073 2036 845
Size: 228 963 2933
Fragments arranged by size:
2933 963 228
BsrFI r'CCGG_y
Cuts at: 185 2122 2303 2512 185
Size: 1937 181 209 1797
Fragments arranged by size:
1937 1797 209 181
BssSI C'ACGA_G
Cuts at: 615 2395 2753 615
Size: 1780 358 1986
Fragments arranged by size:
1986 1780 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 990 2485 990
Size: 1495 2629
BstXI CCAn_nnnn'nTGG
Cuts at: 369 858 369
Size: 489 3635
BstYI r'GATC_y
Cuts at: 1 1055 1149 1616 1639 1974 2220 3232 3318
Size: 1054 94 467 23 335 246 1012 86
Cuts at: 3318 3330 3676 1
Size: 12 346 449
Fragments arranged by size:
1054 1012 467 449 346 335 246 94
86 23 12
BtgI C'CryG_G
Cuts at: 107 143 362 107
Size: 36 219 3869
Fragments arranged by size:
3869 219 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 925 1367 925
Size: 442 3682
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 187 1709 1883 2274 2301 3646 187
Size: 1522 174 391 27 1345 665
Fragments arranged by size:
1522 1345 665 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 519 677 2147 2357 519
Size: 158 1470 210 2286
Fragments arranged by size:
2286 1470 210 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1136 1067
Size: 69 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 934 968 1949 2381 3128 3396 368
Size: 566 34 981 432 747 268 1096
Fragments arranged by size:
1096 981 747 566 432 268 34
EcoO109I rG'GnC_Cy
Cuts at: 1263 1263
Size: 4124
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
EcoRV GAT'ATC
Cuts at: 936 936
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 2143 477
Size: 1666 2458
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 1558 1806 2828 3807 1558
Size: 248 1022 979 1875
Fragments arranged by size:
1875 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 404 436 548 580 858 890 964 996 1966
Size: 32 112 32 278 32 74 32 970
Cuts at: 1966 1996 1998 2028 404
Size: 30 2 30 2500
Fragments arranged by size:
2500 970 278 112 74 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 972 1163 972
Size: 191 3933
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2979 2795
Size: 184 3940
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 778 2113 2240 778
Size: 1335 127 2662
Fragments arranged by size:
2662 1335 127
MspA1I CmG'CkG
Cuts at: 145 611 1062 1235 1549 1909 2922 3167 145
Size: 466 451 173 314 360 1013 245 1102
Fragments arranged by size:
1102 1013 466 451 360 314 245 173
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NcoI C'CATG_G
Cuts at: 107 362 107
Size: 255 3869
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NheI G'CTAG_C
Cuts at: 433 433
Size: 4124
NspI r_CATG'y
Cuts at: 673 747 1177 1677 2584 673
Size: 74 430 500 907 2213
Fragments arranged by size:
2213 907 500 430 74
PasI CC'CwG_GG
Cuts at: 170 292 170
Size: 122 4002
PciI A'CATG_T
Cuts at: 669 2580 669
Size: 1911 2213
PfoI T'CCnGG_A
Cuts at: 857 857
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PstI C_TGCA'G
Cuts at: 1171 1856 1171
Size: 685 3439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1062 1549 1909 1062
Size: 487 360 3277
Fragments arranged by size:
3277 487 360
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1069 1138 1069
Size: 69 4055
SacII CC_GC'GG
Cuts at: 146 146
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SfcI C'TryA_G
Cuts at: 33 894 1167 1852 2845 3036 33
Size: 861 273 685 993 191 1121
Fragments arranged by size:
1121 993 861 685 273 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 124 519 835 1279 2194 2686 2948 124
Size: 395 316 444 915 492 262 1300
Fragments arranged by size:
1300 915 492 444 395 316 262
SpeI A'CTAG_T
Cuts at: 67 720 67
Size: 653 3471
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
SspI AAT'ATT
Cuts at: 449 449
Size: 4124
StyI C'CwwG_G
Cuts at: 107 362 1258 1612 107
Size: 255 896 354 2619
Fragments arranged by size:
2619 896 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 275 407 432 619 1707 1840 2299 2508 275
Size: 132 25 187 1088 133 459 209 1891
Fragments arranged by size:
1891 1088 459 209 187 133 132 25
TatI w'GTAC_w
Cuts at: 1025 3639 1025
Size: 2614 1510
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BglI BmgBI BpmI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI