Restriction map for
pF1KB3493
(Circular) (Six-base) MAPSORT of: pF1KB3493.seq Check: 1202 from: 1 to: 5003
pF1KB3493 5003 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 506 506
Size: 5003
AccI GT'mk_AC
Cuts at: 275 1073 2041 275
Size: 798 968 3237
Fragments arranged by size:
3237 968 798
Acc65I G'GTAC_C
Cuts at: 2019 2019
Size: 5003
AclI AA'CG_TT
Cuts at: 4843 4843
Size: 5003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1171 2828 3260 4007 4275 460
Size: 711 1657 432 747 268 1188
Fragments arranged by size:
1657 1188 747 711 432 268
AflIII A'CryG_T
Cuts at: 2355 3459 4436 2355
Size: 1104 977 2922
Fragments arranged by size:
2922 1104 977
AgeI A'CCGG_T
Cuts at: 3391 3391
Size: 5003
AhdI GACnn_n'nnGTC
Cuts at: 1218 1218
Size: 5003
AleI CACnn'nnGTG
Cuts at: 1834 1834
Size: 5003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 135 135
Size: 5003
AlwNI CAG_nnn'CTG
Cuts at: 336 1002 1611 2308 3875 336
Size: 666 609 697 1567 1464
Fragments arranged by size:
1567 1464 697 666 609
ApaI G_GGCC'C
Cuts at: 1720 1720
Size: 5003
ApaLI G'TGCA_C
Cuts at: 3773 3773
Size: 5003
ApoI r'AATT_y
Cuts at: 601 2007 4356 4612 601
Size: 1406 2349 256 992
Fragments arranged by size:
2349 1406 992 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5003
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
AvaI C'yCGr_G
Cuts at: 353 613 1081 2023 353
Size: 260 468 942 3333
Fragments arranged by size:
3333 942 468 260
BamHI G'GATC_C
Cuts at: 1637 2028 1637
Size: 391 4612
BanI G'GyrC_C
Cuts at: 1969 2019 2433 2681 2716 1969
Size: 50 414 248 35 4256
Fragments arranged by size:
4256 414 248 50 35
BanII G_rGCy'C
Cuts at: 164 244 1181 1332 1463 1720 2017 3047 164
Size: 80 937 151 131 257 297 1030 2120
Fragments arranged by size:
2120 1030 937 297 257 151 131 80
BbeI G_GCGC'C
Cuts at: 2437 2685 2437
Size: 248 4755
BbsI GAAGACnn'nnnn_
Cuts at: 463 463
Size: 5003
BciVI GTATCCnnnnn_n'
Cuts at: 2901 3662 5003 2901
Size: 761 1341 2901
Fragments arranged by size:
2901 1341 761
BclI T'GATC_A
Cuts at: 2523 2523
Size: 5003
BglI GCCn_nnn'nGGC
Cuts at: 349 421 349
Size: 72 4931
BglII A'GATC_T
Cuts at: 1778 2518 4555 1778
Size: 740 2037 2226
Fragments arranged by size:
2226 2037 740
BlpI GC'TnA_GC
Cuts at: 2115 2115
Size: 5003
Bme1580I G_kGCm'C
Cuts at: 124 259 1720 2628 2721 3777 124
Size: 135 1461 908 93 1056 1350
Fragments arranged by size:
1461 1350 1056 908 135 93
BmgBI CAC'GTC
Cuts at: 227 461 227
Size: 234 4769
BmrI ACTGGGnnnn_n'
Cuts at: 1181 1656 2215 2296 2631 4775 1181
Size: 475 559 81 335 2144 1409
Fragments arranged by size:
2144 1409 559 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 897 3370 897
Size: 2473 2530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 721 1051 1156 1223 1450 1486 178
Size: 543 330 105 67 227 36 3695
Fragments arranged by size:
3695 543 330 227 105 67 36
Bpu10I CC'TnA_GC
Cuts at: 679 1457 679
Size: 778 4225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1399 2179 3058 3550 3848 937
Size: 462 780 879 492 298 2092
Fragments arranged by size:
2092 879 780 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 868 1260 1426 4707 868
Size: 392 166 3281 1164
Fragments arranged by size:
3281 1164 392 166
BsaAI yAC'GTr
Cuts at: 2356 2356
Size: 5003
BsaBI GATnn'nnATC
Cuts at: 6 2517 4554 6
Size: 2511 2037 455
Fragments arranged by size:
2511 2037 455
BsaHI Gr'CG_yC
Cuts at: 379 754 866 2434 2682 3384 4880 379
Size: 375 112 1568 248 702 1496 502
Fragments arranged by size:
1568 1496 702 502 375 248 112
BsaWI w'CCGG_w
Cuts at: 2192 2713 3391 3665 3812 4223 2192
Size: 521 678 274 147 411 2972
Fragments arranged by size:
2972 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 1445 1475 1732 1762 127
Size: 30 1288 30 257 30 3368
Fragments arranged by size:
3368 1288 257 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 919 940 1000 148
Size: 771 21 60 4151
Fragments arranged by size:
4151 771 60 21
BseYI C'CCAG_C
Cuts at: 403 581 1215 1306 1611 2428 3186 3763 4259
Size: 178 634 91 305 817 758 577 496
Cuts at: 4259 403
Size: 1147
Fragments arranged by size:
1147 817 758 634 577 496 305 178
91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 383 1603 280
Size: 103 1220 3680
Fragments arranged by size:
3680 1220 103
BsiEI CG_ry'CG
Cuts at: 104 289 1576 1737 2545 2591 3799 104
Size: 185 1287 161 808 46 1208 1308
Fragments arranged by size:
1308 1287 1208 808 185 161 46
BsiHKAI G_wGCw'C
Cuts at: 148 497 620 765 1129 1181 1463 2017 2795
Size: 349 123 145 364 52 282 554 778
Cuts at: 2795 2985 3777 148
Size: 190 792 1374
Fragments arranged by size:
1374 792 778 554 364 349 282 190
145 123 52
BsmI GAATG_Cn'
Cuts at: 417 417
Size: 5003
BsmBI CGTCTCn'nnnn_
Cuts at: 217 217
Size: 5003
Bsp1286I G_dGCh'C
Cuts at: 124 148 164 244 259 497 620 765 1129
Size: 24 16 80 15 238 123 145 364
Cuts at: 1129 1181 1332 1463 1720 2017 2628 2721 2795
Size: 52 151 131 257 297 611 93 74
Cuts at: 2795 2985 3047 3777 124
Size: 190 62 730 1350
Fragments arranged by size:
1350 730 611 364 297 257 238 190
151 145 131 123 93 80 74 62
52 24 16 15
BspEI T'CCGG_A
Cuts at: 4223 4223
Size: 5003
BspHI T'CATG_A
Cuts at: 4179 4179
Size: 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 2053 2267 2569 2950 506
Size: 1547 214 302 381 2559
Fragments arranged by size:
2559 1547 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3295 3349 4833 4998 50
Size: 3245 54 1484 165 55
Fragments arranged by size:
3245 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2915 2915
Size: 5003
BsrFI r'CCGG_y
Cuts at: 174 1571 1709 3001 3182 3391 174
Size: 1397 138 1292 181 209 1786
Fragments arranged by size:
1786 1397 1292 209 181 138
BsrGI T'GTAC_A
Cuts at: 734 734
Size: 5003
BssSI C'ACGA_G
Cuts at: 3274 3632 3274
Size: 358 4645
BstAPI GCAn_nnn'nTGC
Cuts at: 336 795 2380 336
Size: 459 1585 2959
Fragments arranged by size:
2959 1585 459
BstBI TT'CG_AA
Cuts at: 3364 3364
Size: 5003
BstEII G'GTnAC_C
Cuts at: 177 1195 177
Size: 1018 3985
BstXI CCAn_nnnn'nTGG
Cuts at: 156 1428 1836 156
Size: 1272 408 3323
Fragments arranged by size:
3323 1272 408
BstYI r'GATC_y
Cuts at: 1 968 1637 1778 2028 2495 2518 2853 3099
Size: 967 669 141 250 467 23 335 246
Cuts at: 3099 4111 4197 4209 4555 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 967 669 467 449 346 335 250
246 141 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 712 712
Size: 5003
BtgI C'CryG_G
Cuts at: 107 1860 107
Size: 1753 3250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2953 3134 3297 2953
Size: 181 163 4659
Fragments arranged by size:
4659 181 163
BtsI GCAGTG_nn'
Cuts at: 268 432 1162 1620 2246 268
Size: 164 730 458 626 3025
Fragments arranged by size:
3025 730 626 458 164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1659 1694 1659
Size: 35 4968
DraI TTT'AAA
Cuts at: 1352 2002 1352
Size: 650 4353
DraIII CAC_nnn'GTG
Cuts at: 188 188
Size: 5003
DrdI GACnn_nn'nnGTC
Cuts at: 2709 3567 2709
Size: 858 4145
EaeI y'GGCC_r
Cuts at: 128 192 1442 1573 1734 2588 2762 3153 3180
Size: 64 1250 131 161 854 174 391 27
Cuts at: 3180 4525 128
Size: 1345 606
Fragments arranged by size:
1345 1250 854 606 391 174 161 131
64 27
EagI C'GGCC_G
Cuts at: 1573 1734 2588 1573
Size: 161 854 3988
Fragments arranged by size:
3988 854 161
EarI CTCTTCn'nnn_
Cuts at: 896 904 955 3026 3236 896
Size: 8 51 2071 210 2663
Fragments arranged by size:
2663 2071 210 51 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3211 3521 3667 4813 3211
Size: 310 146 1146 3401
Fragments arranged by size:
3401 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1179 1461 2015 1179
Size: 282 554 4167
Fragments arranged by size:
4167 554 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 460 721 1051 1156 1171 1223 1450 1486
Size: 282 261 330 105 15 52 227 36
Cuts at: 1486 2828 3260 4007 4275 178
Size: 1342 432 747 268 906
Fragments arranged by size:
1342 906 747 432 330 282 268 261
227 105 52 36 15
EcoNI CCTnn'n_nnAGG
Cuts at: 130 442 682 885 130
Size: 312 240 203 4248
Fragments arranged by size:
4248 312 240 203
EcoO109I rG'GnC_Cy
Cuts at: 1716 2142 1716
Size: 426 4577
EcoRI G'AATT_C
Cuts at: 2007 2007
Size: 5003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 480 876 1689 3022 480
Size: 396 813 1333 2461
Fragments arranged by size:
2461 1333 813 396
FspI TGC'GCA
Cuts at: 2784 2784
Size: 5003
HaeII r_GCGC'y
Cuts at: 695 1748 2437 2685 3707 4686 695
Size: 1053 689 248 1022 979 1012
Fragments arranged by size:
1053 1022 1012 979 689 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 1024 1056 1204 1236 1445 1477 2845
Size: 32 868 32 148 32 209 32 1368
Cuts at: 2845 2875 2877 2907 124
Size: 30 2 30 2220
Fragments arranged by size:
2220 1368 868 209 148 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 918 2042 918
Size: 1124 3879
HindIII A'AGCT_T
Cuts at: 1312 1312
Size: 5003
KasI G'GCGC_C
Cuts at: 2433 2681 2433
Size: 248 4755
KpnI G_GTAC'C
Cuts at: 2023 2023
Size: 5003
MluI A'CGCG_T
Cuts at: 4436 4436
Size: 5003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3674 3858 3674
Size: 184 4819
MscI TGG'CCA
Cuts at: 1444 2764 1444
Size: 1320 3683
MslI CAynn'nnrTG
Cuts at: 252 847 1834 2992 3119 252
Size: 595 987 1158 127 2136
Fragments arranged by size:
2136 1158 987 595 127
MspA1I CmG'CkG
Cuts at: 294 540 2114 2428 2788 3801 4046 294
Size: 246 1574 314 360 1013 245 1251
Fragments arranged by size:
1574 1251 1013 360 314 246 245
NaeI GCC'GGC
Cuts at: 3184 3184
Size: 5003
NarI GG'CG_CC
Cuts at: 2434 2682 2434
Size: 248 4755
NcoI C'CATG_G
Cuts at: 107 107
Size: 5003
NgoMIV G'CCGG_C
Cuts at: 3182 3182
Size: 5003
NruI TCG'CGA
Cuts at: 557 557
Size: 5003
NspI r_CATG'y
Cuts at: 2056 2556 3463 2056
Size: 500 907 3596
Fragments arranged by size:
3596 907 500
PciI A'CATG_T
Cuts at: 3459 3459
Size: 5003
PfoI T'CCnGG_A
Cuts at: 527 1172 1463 1487 527
Size: 645 291 24 4043
Fragments arranged by size:
4043 645 291 24
PmeI GTTT'AAAC
Cuts at: 2002 2002
Size: 5003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4971
PspOMI G'GGCC_C
Cuts at: 1716 1716
Size: 5003
PstI C_TGCA'G
Cuts at: 341 503 545 1377 1664 2050 2735 341
Size: 162 42 832 287 386 685 2609
Fragments arranged by size:
2609 832 685 386 287 162 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5003
PvuII CAG'CTG
Cuts at: 294 2428 2788 294
Size: 2134 360 2509
Fragments arranged by size:
2509 2134 360
RsrII CG'GwC_CG
Cuts at: 3198 3198
Size: 5003
SacI G_AGCT'C
Cuts at: 1181 1463 2017 1181
Size: 282 554 4167
Fragments arranged by size:
4167 554 282
SalI G'TCGA_C
Cuts at: 2040 2040
Size: 5003
SapI GCTCTTCn'nnn_
Cuts at: 3026 3236 3026
Size: 210 4793
SbfI CC_TGCA'GG
Cuts at: 341 2050 341
Size: 1709 3294
ScaI AGT'ACT
Cuts at: 4520 4520
Size: 5003
SfcI C'TryA_G
Cuts at: 33 337 499 541 1373 1660 2046 2731 3724
Size: 304 162 42 832 287 386 685 993
Cuts at: 3724 3915 33
Size: 191 1121
Fragments arranged by size:
1121 993 832 685 386 304 287 191
162 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 349 349
Size: 5003
SfoI GGC'GCC
Cuts at: 2435 2683 2435
Size: 248 4755
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
SmaI CCC'GGG
Cuts at: 355 2025 355
Size: 1670 3333
SmlI C'TyrA_G
Cuts at: 952 1414 2158 3073 3565 3827 952
Size: 462 744 915 492 262 2128
Fragments arranged by size:
2128 915 744 492 462 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5003
SphI G_CATG'C
Cuts at: 2056 2556 2056
Size: 500 4503
StuI AGG'CCT
Cuts at: 661 661
Size: 5003
StyI C'CwwG_G
Cuts at: 107 1421 1823 1994 2137 2491 107
Size: 1314 402 171 143 354 2619
Fragments arranged by size:
2619 1314 402 354 171 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 417 2586 2719 3178 3387 417
Size: 2169 133 459 209 2033
Fragments arranged by size:
2169 2033 459 209 133
TatI w'GTAC_w
Cuts at: 734 4518 734
Size: 3784 1219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 4520 4552 127
Size: 32 4361 32 578
Fragments arranged by size:
4361 578 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 2800 1894
Size: 906 4097
XbaI T'CTAG_A
Cuts at: 2034 2034
Size: 5003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 696 2498 696
Size: 1802 3201
XmaI C'CCGG_G
Cuts at: 353 2023 353
Size: 1670 3333
XmnI GAAnn'nnTTC
Cuts at: 1672 1672
Size: 5003
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsiWI BssHII BstZ17I ClaI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI