Restriction map for
pF1KB3497
(Circular) (Six-base) MAPSORT of: pF1KB3497.seq Check: 8673 from: 1 to: 7424
pF1KB3497 7424 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3474 3474
Size: 7424
AccI GT'mk_AC
Cuts at: 1276 4462 1276
Size: 3186 4238
Acc65I G'GTAC_C
Cuts at: 522 4440 522
Size: 3918 3506
AclI AA'CG_TT
Cuts at: 3246 7264 3246
Size: 4018 3406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 752 1325 1618 1814 2108 3209 5249 5681
Size: 501 573 293 196 294 1101 2040 432
Cuts at: 5681 6428 6696 251
Size: 747 268 979
Fragments arranged by size:
2040 1101 979 747 573 501 432 294
293 268 196
AflIII A'CryG_T
Cuts at: 1595 4776 5880 6857 1595
Size: 3181 1104 977 2162
Fragments arranged by size:
3181 2162 1104 977
AgeI A'CCGG_T
Cuts at: 5812 5812
Size: 7424
AleI CACnn'nnGTG
Cuts at: 1475 3807 1475
Size: 2332 5092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1493 2947 3057 3960 1493
Size: 1454 110 903 4957
Fragments arranged by size:
4957 1454 903 110
AlwNI CAG_nnn'CTG
Cuts at: 2131 4729 6296 2131
Size: 2598 1567 3259
Fragments arranged by size:
3259 2598 1567
ApaI G_GGCC'C
Cuts at: 4287 4287
Size: 7424
ApaLI G'TGCA_C
Cuts at: 6194 6194
Size: 7424
ApoI r'AATT_y
Cuts at: 452 476 1445 1657 2422 2449 3613 4243 4428
Size: 24 969 212 765 27 1164 630 185
Cuts at: 4428 6777 7033 452
Size: 2349 256 843
Fragments arranged by size:
2349 1164 969 843 765 630 256 212
185 27 24
AseI AT'TA_AT
Cuts at: 20 3640 20
Size: 3620 3804
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7424
AvaI C'yCGr_G
Cuts at: 1022 1253 4444 1022
Size: 231 3191 4002
Fragments arranged by size:
4002 3191 231
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1078 1111 1078
Size: 33 7391
BamHI G'GATC_C
Cuts at: 4449 4449
Size: 7424
BanI G'GyrC_C
Cuts at: 522 1018 1963 4440 4854 5102 5137 522
Size: 496 945 2477 414 248 35 2809
Fragments arranged by size:
2809 2477 945 496 414 248 35
BanII G_rGCy'C
Cuts at: 3681 4287 4438 5468 3681
Size: 606 151 1030 5637
Fragments arranged by size:
5637 1030 606 151
BbeI G_GCGC'C
Cuts at: 4858 5106 4858
Size: 248 7176
BbsI GAAGACnn'nnnn_
Cuts at: 553 608 1951 553
Size: 55 1343 6026
Fragments arranged by size:
6026 1343 55
BbvCI CC'TCA_GC
Cuts at: 1130 2492 1130
Size: 1362 6062
BciVI GTATCCnnnnn_n'
Cuts at: 335 392 2637 5322 6083 7424 335
Size: 57 2245 2685 761 1341 335
Fragments arranged by size:
2685 2245 1341 761 335 57
BclI T'GATC_A
Cuts at: 4944 4944
Size: 7424
BfrBI ATG'CAT
Cuts at: 4065 4065
Size: 7424
BglII A'GATC_T
Cuts at: 300 727 1375 3038 4939 6976 300
Size: 427 648 1663 1901 2037 748
Fragments arranged by size:
2037 1901 1663 748 648 427
BlpI GC'TnA_GC
Cuts at: 4536 4536
Size: 7424
Bme1580I G_kGCm'C
Cuts at: 1023 2896 3493 4287 5049 5142 6198 1023
Size: 1873 597 794 762 93 1056 2249
Fragments arranged by size:
2249 1873 1056 794 762 597 93
BmrI ACTGGGnnnn_n'
Cuts at: 847 2357 3144 4636 4717 5052 7196 847
Size: 1510 787 1492 81 335 2144 1075
Fragments arranged by size:
2144 1510 1492 1075 787 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1440 5791 1440
Size: 4351 3073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1586 2308 2612 3518 4383 1586
Size: 722 304 906 865 4627
Fragments arranged by size:
4627 906 865 722 304
Bpu10I CC'TnA_GC
Cuts at: 1130 2492 2920 1130
Size: 1362 428 5634
Fragments arranged by size:
5634 1362 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1419 3291 4600 5479 5971 6269 1009
Size: 410 1872 1309 879 492 298 2164
Fragments arranged by size:
2164 1872 1309 879 492 410 298
BsaI GGTCTCn'nnnn_
Cuts at: 623 7128 623
Size: 6505 919
BsaAI yAC'GTr
Cuts at: 4777 4777
Size: 7424
BsaBI GATnn'nnATC
Cuts at: 6 2304 4938 6975 6
Size: 2298 2634 2037 455
Fragments arranged by size:
2634 2298 2037 455
BsaHI Gr'CG_yC
Cuts at: 4855 5103 5805 7301 4855
Size: 248 702 1496 4978
Fragments arranged by size:
4978 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4613 5134 5812 6086 6233 6644 4613
Size: 521 678 274 147 411 5393
Fragments arranged by size:
5393 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4091 4121 4180 4210 4394 4424 4091
Size: 30 59 30 184 30 7091
Fragments arranged by size:
7091 184 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 503 1416 503
Size: 913 6511
BseYI C'CCAG_C
Cuts at: 285 983 2895 3492 3575 4849 5607 6184 6680
Size: 698 1912 597 83 1274 758 577 496
Cuts at: 6680 285
Size: 1029
Fragments arranged by size:
1912 1274 1029 758 698 597 577 496
83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 2176 3382 4236 4244 4283 431
Size: 1745 1206 854 8 39 3572
Fragments arranged by size:
3572 1745 1206 854 39 8
BsiEI CG_ry'CG
Cuts at: 104 2612 4966 5012 6220 104
Size: 2508 2354 46 1208 1308
Fragments arranged by size:
2508 2354 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 662 1206 4438 5216 5406 6198 662
Size: 544 3232 778 190 792 1888
Fragments arranged by size:
3232 1888 792 778 544 190
BsmI GAATG_Cn'
Cuts at: 1644 2920 3478 3661 1644
Size: 1276 558 183 5407
Fragments arranged by size:
5407 1276 558 183
BsmBI CGTCTCn'nnnn_
Cuts at: 4026 4026
Size: 7424
Bsp1286I G_dGCh'C
Cuts at: 662 1023 1206 2896 3493 3681 4287 4438 5049
Size: 361 183 1690 597 188 606 151 611
Cuts at: 5049 5142 5216 5406 5468 6198 662
Size: 93 74 190 62 730 1888
Fragments arranged by size:
1888 1690 730 611 606 597 361 190
188 183 151 93 74 62
BspEI T'CCGG_A
Cuts at: 6644 6644
Size: 7424
BspHI T'CATG_A
Cuts at: 1074 2430 3123 3770 4272 6600 1074
Size: 1356 693 647 502 2328 1898
Fragments arranged by size:
2328 1898 1356 693 647 502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1905 2082 2096 3474 4474 4688 4990 5371 1905
Size: 177 14 1378 1000 214 302 381 3958
Fragments arranged by size:
3958 1378 1000 381 302 214 177 14
BsrBI CCG'CTC
Cuts at: 50 3567 5716 5770 7254 7419 50
Size: 3517 2149 54 1484 165 55
Fragments arranged by size:
3517 2149 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 224 1220 2603 5336 224
Size: 996 1383 2733 2312
Fragments arranged by size:
2733 2312 1383 996
BsrFI r'CCGG_y
Cuts at: 3742 5422 5603 5812 3742
Size: 1680 181 209 5354
Fragments arranged by size:
5354 1680 209 181
BssSI C'ACGA_G
Cuts at: 3682 5695 6053 3682
Size: 2013 358 5053
Fragments arranged by size:
5053 2013 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4012 4801 4012
Size: 789 6635
BstBI TT'CG_AA
Cuts at: 5785 5785
Size: 7424
BstXI CCAn_nnnn'nTGG
Cuts at: 4361 4361
Size: 7424
BstYI r'GATC_y
Cuts at: 1 300 727 1375 3038 3365 4449 4916 4939
Size: 299 427 648 1663 327 1084 467 23
Cuts at: 4939 5274 5520 6532 6618 6630 6976 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1663 1084 1012 648 467 449 427 346
335 327 299 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1277 1277
Size: 7424
Bsu36I CC'TnA_GG
Cuts at: 1931 3583 1931
Size: 1652 5772
BtgI C'CryG_G
Cuts at: 107 2162 4195 4337 107
Size: 2055 2033 142 3194
Fragments arranged by size:
3194 2055 2033 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4223 5374 5555 5718 4223
Size: 1151 181 163 5929
Fragments arranged by size:
5929 1151 181 163
BtsI GCAGTG_nn'
Cuts at: 2456 2496 3954 4227 4667 2456
Size: 40 1458 273 440 5213
Fragments arranged by size:
5213 1458 440 273 40
ClaI AT'CG_AT
Cuts at: 1664 1664
Size: 7424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1866 1901 1866
Size: 35 7389
DraI TTT'AAA
Cuts at: 638 4423 638
Size: 3785 3639
DrdI GACnn_nn'nnGTC
Cuts at: 5130 5988 5130
Size: 858 6566
EaeI y'GGCC_r
Cuts at: 240 2609 3028 3967 5009 5183 5574 5601 6946
Size: 2369 419 939 1042 174 391 27 1345
Cuts at: 6946 240
Size: 718
Fragments arranged by size:
2369 1345 1042 939 718 419 391 174
27
EagI C'GGCC_G
Cuts at: 2609 5009 2609
Size: 2400 5024
EarI CTCTTCn'nnn_
Cuts at: 728 1301 1478 1484 1637 2178 3992 4024 5447
Size: 573 177 6 153 541 1814 32 1423
Cuts at: 5447 5657 728
Size: 210 2495
Fragments arranged by size:
2495 1814 1423 573 541 210 177 153
32 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 171 5632 5942 6088 7234 171
Size: 5461 310 146 1146 361
Fragments arranged by size:
5461 1146 361 310 146
EcoICRI GAG'CTC
Cuts at: 4436 4436
Size: 7424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 752 1325 1586 1618 1814 2108 2308 2612
Size: 501 573 261 32 196 294 200 304
Cuts at: 2612 3209 3518 4383 5249 5681 6428 6696 251
Size: 597 309 865 866 432 747 268 979
Fragments arranged by size:
979 866 865 747 597 573 501 432
309 304 294 268 261 200 196 32
EcoO109I rG'GnC_Cy
Cuts at: 3075 3579 4283 4284 4563 3075
Size: 504 704 1 279 5936
Fragments arranged by size:
5936 704 504 279 1
EcoRI G'AATT_C
Cuts at: 4243 4428 4243
Size: 185 7239
EcoRV GAT'ATC
Cuts at: 2529 2529
Size: 7424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1750 5443 1750
Size: 3693 3731
FspI TGC'GCA
Cuts at: 5205 5205
Size: 7424
HaeII r_GCGC'y
Cuts at: 4858 5106 6128 7107 4858
Size: 248 1022 979 5175
Fragments arranged by size:
5175 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 793 825 1300 1332 2143 2175 3653
Size: 32 34 32 475 32 811 32 1478
Cuts at: 3653 3685 4025 4057 4091 4123 5266 5296 5298
Size: 32 340 32 34 32 1143 30 2
Cuts at: 5298 5328 727
Size: 30 2823
Fragments arranged by size:
2823 1478 1143 811 475 340 34 34
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 801 3713 3878 3989 4463 801
Size: 2912 165 111 474 3762
Fragments arranged by size:
3762 2912 474 165 111
HindIII A'AGCT_T
Cuts at: 3180 3180
Size: 7424
HpaI GTT'AAC
Cuts at: 3713 3878 3713
Size: 165 7259
KasI G'GCGC_C
Cuts at: 4854 5102 4854
Size: 248 7176
KpnI G_GTAC'C
Cuts at: 526 4444 526
Size: 3918 3506
MluI A'CGCG_T
Cuts at: 6857 6857
Size: 7424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 904 6095 6279 904
Size: 5191 184 2049
Fragments arranged by size:
5191 2049 184
MscI TGG'CCA
Cuts at: 242 3030 5185 242
Size: 2788 2155 2481
Fragments arranged by size:
2788 2481 2155
MslI CAynn'nnrTG
Cuts at: 1475 1558 2194 2332 2929 3807 5413 5540 1475
Size: 83 636 138 597 878 1606 127 3359
Fragments arranged by size:
3359 1606 878 636 597 138 127 83
MspA1I CmG'CkG
Cuts at: 2486 3398 3415 3496 4228 4535 4849 5209 6222
Size: 912 17 81 732 307 314 360 1013
Cuts at: 6222 6467 2486
Size: 245 3443
Fragments arranged by size:
3443 1013 912 732 360 314 307 245
81 17
NaeI GCC'GGC
Cuts at: 3744 5605 3744
Size: 1861 5563
NarI GG'CG_CC
Cuts at: 4855 5103 4855
Size: 248 7176
NcoI C'CATG_G
Cuts at: 107 2162 4337 107
Size: 2055 2175 3194
Fragments arranged by size:
3194 2175 2055
NdeI CA'TA_TG
Cuts at: 252 2760 4176 252
Size: 2508 1416 3500
Fragments arranged by size:
3500 2508 1416
NgoMIV G'CCGG_C
Cuts at: 3742 5603 3742
Size: 1861 5563
NsiI A_TGCA'T
Cuts at: 4067 4067
Size: 7424
NspI r_CATG'y
Cuts at: 1599 2622 3983 4477 4977 5884 1599
Size: 1023 1361 494 500 907 3139
Fragments arranged by size:
3139 1361 1023 907 500 494
PciI A'CATG_T
Cuts at: 1595 5880 1595
Size: 4285 3139
PflMI CCAn_nnn'nTGG
Cuts at: 4177 4177
Size: 7424
PfoI T'CCnGG_A
Cuts at: 3251 3251
Size: 7424
PmeI GTTT'AAAC
Cuts at: 4423 4423
Size: 7424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6563 6595 6563
Size: 32 7392
PsiI TTA'TAA
Cuts at: 1738 3890 1738
Size: 2152 5272
PspOMI G'GGCC_C
Cuts at: 4283 4283
Size: 7424
PstI C_TGCA'G
Cuts at: 2093 2136 3867 3949 4471 5156 2093
Size: 43 1731 82 522 685 4361
Fragments arranged by size:
4361 1731 685 522 82 43
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7424
PvuII CAG'CTG
Cuts at: 3415 3496 4228 4849 5209 3415
Size: 81 732 621 360 5630
Fragments arranged by size:
5630 732 621 360 81
RsrII CG'GwC_CG
Cuts at: 5619 5619
Size: 7424
SacI G_AGCT'C
Cuts at: 4438 4438
Size: 7424
SalI G'TCGA_C
Cuts at: 4461 4461
Size: 7424
SapI GCTCTTCn'nnn_
Cuts at: 728 1484 1637 5447 5657 728
Size: 756 153 3810 210 2495
Fragments arranged by size:
3810 2495 756 210 153
SbfI CC_TGCA'GG
Cuts at: 2093 3867 4471 2093
Size: 1774 604 5046
Fragments arranged by size:
5046 1774 604
ScaI AGT'ACT
Cuts at: 836 1440 6941 836
Size: 604 5501 1319
Fragments arranged by size:
5501 1319 604
SfcI C'TryA_G
Cuts at: 33 1643 1811 1937 2089 2132 2646 3863 3945
Size: 1610 168 126 152 43 514 1217 82
Cuts at: 3945 4467 5152 6145 6336 33
Size: 522 685 993 191 1121
Fragments arranged by size:
1610 1217 1121 993 685 522 514 191
168 152 126 82 43
SfoI GGC'GCC
Cuts at: 4856 5104 4856
Size: 248 7176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7424
SmaI CCC'GGG
Cuts at: 4446 4446
Size: 7424
SmlI C'TyrA_G
Cuts at: 988 1253 1434 3306 4579 5494 5986 6248 988
Size: 265 181 1872 1273 915 492 262 2164
Fragments arranged by size:
2164 1872 1273 915 492 265 262 181
SpeI A'CTAG_T
Cuts at: 67 2035 3199 67
Size: 1968 1164 4292
Fragments arranged by size:
4292 1968 1164
SphI G_CATG'C
Cuts at: 4477 4977 4477
Size: 500 6924
StyI C'CwwG_G
Cuts at: 107 820 935 952 2162 3722 4337 4558 4912
Size: 713 115 17 1210 1560 615 221 354
Cuts at: 4912 107
Size: 2619
Fragments arranged by size:
2619 1560 1210 713 615 354 221 115
17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2372 5007 5140 5599 5808 2372
Size: 2635 133 459 209 3988
Fragments arranged by size:
3988 2635 459 209 133
TatI w'GTAC_w
Cuts at: 645 834 1438 6939 645
Size: 189 604 5501 1130
Fragments arranged by size:
5501 1130 604 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 737 769 3053 3085 3430 3462 4180 4212 6941
Size: 32 2284 32 345 32 718 32 2729
Cuts at: 6941 6973 737
Size: 32 1188
Fragments arranged by size:
2729 2284 1188 718 345 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5221 5221
Size: 7424
XbaI T'CTAG_A
Cuts at: 2071 4455 2071
Size: 2384 5040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1064 2942 4181 4919 1064
Size: 1878 1239 738 3569
Fragments arranged by size:
3569 1878 1239 738
XhoI C'TCGA_G
Cuts at: 1253 1253
Size: 7424
XmaI C'CCGG_G
Cuts at: 4444 4444
Size: 7424
XmnI GAAnn'nnTTC
Cuts at: 302 1857 1925 3759 302
Size: 1555 68 1834 3967
Fragments arranged by size:
3967 1834 1555 68
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BcgI
BglI BmgBI BmtI BsiWI BsrGI BssHII BstEII DraIII
EcoNI FseI FspAI MfeI NheI NotI NruI PacI
PasI PmlI PpuMI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI