Restriction map for
pF1KB3499
(Circular) (Six-base) MAPSORT of: pF1KB3499.seq Check: 3917 from: 1 to: 6329
pF1KB3499 6329 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1325 1715 1786 1939 1325
Size: 390 71 153 5715
Fragments arranged by size:
5715 390 153 71
AatII G_ACGT'C
Cuts at: 124 124
Size: 6329
AccI GT'mk_AC
Cuts at: 470 542 935 3367 470
Size: 72 393 2432 3432
Fragments arranged by size:
3432 2432 393 72
Acc65I G'GTAC_C
Cuts at: 1017 3345 1017
Size: 2328 4001
AclI AA'CG_TT
Cuts at: 6169 6169
Size: 6329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2464 3019 4154 4586 5333 5601 2464
Size: 555 1135 432 747 268 3192
Fragments arranged by size:
3192 1135 747 555 432 268
AflIII A'CryG_T
Cuts at: 1289 2463 3681 4785 5762 1289
Size: 1174 1218 1104 977 1856
Fragments arranged by size:
1856 1218 1174 1104 977
AgeI A'CCGG_T
Cuts at: 4717 4717
Size: 6329
AhdI GACnn_n'nnGTC
Cuts at: 2424 2424
Size: 6329
AleI CACnn'nnGTG
Cuts at: 982 2368 982
Size: 1386 4943
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 271 361 1162 271
Size: 90 801 5438
Fragments arranged by size:
5438 801 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2203 2235 2203
Size: 32 6297
AlwNI CAG_nnn'CTG
Cuts at: 2029 2700 3634 5201 2029
Size: 671 934 1567 3157
Fragments arranged by size:
3157 1567 934 671
ApaI G_GGCC'C
Cuts at: 792 1083 1258 2643 792
Size: 291 175 1385 4478
Fragments arranged by size:
4478 1385 291 175
ApaLI G'TGCA_C
Cuts at: 834 841 5099 834
Size: 7 4258 2064
Fragments arranged by size:
4258 2064 7
ApoI r'AATT_y
Cuts at: 2260 2308 3299 3333 5682 5938 2260
Size: 48 991 34 2349 256 2651
Fragments arranged by size:
2651 2349 991 256 48 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 6329
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6329
AvaI C'yCGr_G
Cuts at: 201 593 1066 1491 1904 2403 2795 3254 3349
Size: 392 473 425 413 499 392 459 95
Cuts at: 3349 201
Size: 3181
Fragments arranged by size:
3181 499 473 459 425 413 392 392
95
AvrII C'CTAG_G
Cuts at: 2946 2946
Size: 6329
BamHI G'GATC_C
Cuts at: 1496 1986 2247 3354 1496
Size: 490 261 1107 4471
Fragments arranged by size:
4471 1107 490 261
BanI G'GyrC_C
Cuts at: 127 294 655 873 912 1017 1444 1927 2179
Size: 167 361 218 39 105 427 483 252
Cuts at: 2179 3345 3759 4007 4042 127
Size: 1166 414 248 35 2414
Fragments arranged by size:
2414 1166 483 427 414 361 252 248
218 167 105 39 35
BanII G_rGCy'C
Cuts at: 602 792 1083 1258 1356 1714 1904 1973 2643
Size: 190 291 175 98 358 190 69 670
Cuts at: 2643 3261 3343 4373 602
Size: 618 82 1030 2558
Fragments arranged by size:
2558 1030 670 618 358 291 190 190
175 98 82 69
BbeI G_GCGC'C
Cuts at: 916 3763 4011 916
Size: 2847 248 3234
Fragments arranged by size:
3234 2847 248
BbsI GAAGACnn'nnnn_
Cuts at: 474 993 1982 3007 474
Size: 519 989 1025 3796
Fragments arranged by size:
3796 1025 989 519
BbvCI CC'TCA_GC
Cuts at: 1476 2118 2756 1476
Size: 642 638 5049
Fragments arranged by size:
5049 642 638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2001 2035 2394 2428 2001
Size: 34 359 34 5902
Fragments arranged by size:
5902 359 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4227 4988 6329 4227
Size: 761 1341 4227
Fragments arranged by size:
4227 1341 761
BclI T'GATC_A
Cuts at: 2880 3849 2880
Size: 969 5360
BfrBI ATG'CAT
Cuts at: 3310 3310
Size: 6329
BglI GCCn_nnn'nGGC
Cuts at: 1642 1808 1868 2498 1642
Size: 166 60 630 5473
Fragments arranged by size:
5473 630 166 60
BglII A'GATC_T
Cuts at: 2678 3844 5881 2678
Size: 1166 2037 3126
Fragments arranged by size:
3126 2037 1166
BlpI GC'TnA_GC
Cuts at: 3441 3441
Size: 6329
Bme1580I G_kGCm'C
Cuts at: 299 537 700 792 838 845 878 1083 1258
Size: 238 163 92 46 7 33 205 175
Cuts at: 1258 2184 2643 3954 4047 5103 299
Size: 926 459 1311 93 1056 1525
Fragments arranged by size:
1525 1311 1056 926 459 238 205 175
163 93 92 46 33 7
BmgBI CAC'GTC
Cuts at: 1014 1014
Size: 6329
BmrI ACTGGGnnnn_n'
Cuts at: 225 1316 3541 3622 3957 6101 225
Size: 1091 2225 81 335 2144 453
Fragments arranged by size:
2225 2144 1091 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 924 1506 2415 4696 924
Size: 582 909 2281 2557
Fragments arranged by size:
2557 2281 909 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 790 1705 2263 2595 3142 748
Size: 42 915 558 332 547 3935
Fragments arranged by size:
3935 915 558 547 332 42
Bpu10I CC'TnA_GC
Cuts at: 1476 2118 2756 2978 1476
Size: 642 638 222 4827
Fragments arranged by size:
4827 642 638 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 571 3505 4384 4876 5174 460
Size: 111 2934 879 492 298 1615
Fragments arranged by size:
2934 1615 879 492 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 1111 1822 2223 2411 6033 1111
Size: 711 401 188 3622 1407
Fragments arranged by size:
3622 1407 711 401 188
BsaAI yAC'GTr
Cuts at: 332 2823 3682 332
Size: 2491 859 2979
Fragments arranged by size:
2979 2491 859
BsaBI GATnn'nnATC
Cuts at: 6 367 3843 5880 6
Size: 361 3476 2037 455
Fragments arranged by size:
3476 2037 455 361
BsaHI Gr'CG_yC
Cuts at: 121 281 913 932 1268 3166 3760 4008 4710
Size: 160 632 19 336 1898 594 248 702
Cuts at: 4710 6206 121
Size: 1496 244
Fragments arranged by size:
1898 1496 702 632 594 336 248 244
160 19
BsaWI w'CCGG_w
Cuts at: 124 401 1184 2135 2176 3011 3518 4039 4717
Size: 277 783 951 41 835 507 521 678
Cuts at: 4717 4991 5138 5549 124
Size: 274 147 411 904
Fragments arranged by size:
951 904 835 783 678 521 507 411
277 274 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 403 433 1253 1283 1321 1351 1767
Size: 30 162 30 820 30 38 30 416
Cuts at: 1767 1797 1942 1972 2203 2233 2599 2629 2823
Size: 30 145 30 231 30 366 30 194
Cuts at: 2823 2853 211
Size: 30 3687
Fragments arranged by size:
3687 820 416 366 231 194 162 145
38 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 379 874 1654 1708 1963 1966 2106 2160
Size: 75 495 780 54 255 3 140 54
Cuts at: 2160 2497 2500 2844 3055 3156 3226 3229 304
Size: 337 3 344 211 101 70 3 3404
Fragments arranged by size:
3404 780 495 344 337 255 211 140
101 75 70 54 54 3 3 3
BseYI C'CCAG_C
Cuts at: 298 785 1082 1601 1786 3754 4512 5089 5585
Size: 487 297 519 185 1968 758 577 496
Cuts at: 5585 298
Size: 1042
Fragments arranged by size:
1968 1042 758 577 519 496 487 297
185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1297 1685 706
Size: 591 388 5350
Fragments arranged by size:
5350 591 388
BsiEI CG_ry'CG
Cuts at: 104 1157 3871 3917 5125 104
Size: 1053 2714 46 1208 1308
Fragments arranged by size:
2714 1308 1208 1053 46
BsiHKAI G_wGCw'C
Cuts at: 602 781 838 845 3343 4121 4311 5103 602
Size: 179 57 7 2498 778 190 792 1828
Fragments arranged by size:
2498 1828 792 778 190 179 57 7
BsmI GAATG_Cn'
Cuts at: 632 1434 632
Size: 802 5527
BsmBI CGTCTCn'nnnn_
Cuts at: 1671 2055 1671
Size: 384 5945
Bsp1286I G_dGCh'C
Cuts at: 299 537 602 700 781 792 838 845 878
Size: 238 65 98 81 11 46 7 33
Cuts at: 878 1083 1258 1356 1714 1904 1973 2184 2643
Size: 205 175 98 358 190 69 211 459
Cuts at: 2643 3261 3343 3954 4047 4121 4311 4373 5103
Size: 618 82 611 93 74 190 62 730
Cuts at: 5103 299
Size: 1525
Fragments arranged by size:
1525 730 618 611 459 358 238 211
205 190 190 175 98 98 93 82
81 74 69 65 62 46 33 11
7
BspEI T'CCGG_A
Cuts at: 2135 5549 2135
Size: 3414 2915
BspHI T'CATG_A
Cuts at: 5505 5505
Size: 6329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1029 1325 1715 1786 1807 1939 2429 2439 3379
Size: 296 390 71 21 132 490 10 940
Cuts at: 3379 3593 3895 4276 1029
Size: 214 302 381 3082
Fragments arranged by size:
3082 940 490 390 381 302 296 214
132 71 21 10
BsrBI CCG'CTC
Cuts at: 50 1264 2505 2637 2982 4621 4675 6159 6324
Size: 1214 1241 132 345 1639 54 1484 165
Cuts at: 6324 50
Size: 55
Fragments arranged by size:
1639 1484 1241 1214 345 165 132 55
54
BsrDI GCAATG_nn'
Cuts at: 3070 4241 3070
Size: 1171 5158
BsrFI r'CCGG_y
Cuts at: 244 382 847 1302 2186 3004 3093 4327 4508
Size: 138 465 455 884 818 89 1234 181
Cuts at: 4508 4717 244
Size: 209 1856
Fragments arranged by size:
1856 1234 884 818 465 455 209 181
138 89
BssHII G'CGCG_C
Cuts at: 851 853 851
Size: 2 6327
BssSI C'ACGA_G
Cuts at: 1888 4600 4958 1888
Size: 2712 358 3259
Fragments arranged by size:
3259 2712 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2081 3706 2081
Size: 1625 4704
BstBI TT'CG_AA
Cuts at: 4690 4690
Size: 6329
BstEII G'GTnAC_C
Cuts at: 885 1470 885
Size: 585 5744
BstXI CCAn_nnnn'nTGG
Cuts at: 951 1221 2619 2855 951
Size: 270 1398 236 4425
Fragments arranged by size:
4425 1398 270 236
BstYI r'GATC_y
Cuts at: 1 362 1496 1986 2237 2247 2678 3354 3821
Size: 361 1134 490 251 10 431 676 467
Cuts at: 3821 3844 4179 4425 5437 5523 5535 5881 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1134 1012 676 490 467 449 431 361
346 335 251 246 86 23 12 10
BstZ17I GTA'TAC
Cuts at: 543 543
Size: 6329
Bsu36I CC'TnA_GG
Cuts at: 287 1853 1946 287
Size: 1566 93 4670
Fragments arranged by size:
4670 1566 93
BtgI C'CryG_G
Cuts at: 107 131 447 926 950 980 2204 2222 2366
Size: 24 316 479 24 30 1224 18 144
Cuts at: 2366 2648 107
Size: 282 3788
Fragments arranged by size:
3788 1224 479 316 282 144 30 24
24 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1832 4279 4460 4623 1832
Size: 2447 181 163 3538
Fragments arranged by size:
3538 2447 181 163
BtsI GCAGTG_nn'
Cuts at: 1812 2079 2598 3572 1812
Size: 267 519 974 4569
Fragments arranged by size:
4569 974 519 267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3258 3293 3258
Size: 35 6294
DraI TTT'AAA
Cuts at: 3328 3328
Size: 6329
DrdI GACnn_nn'nnGTC
Cuts at: 4035 4893 4035
Size: 858 5471
EaeI y'GGCC_r
Cuts at: 523 827 986 1565 1634 1809 2372 2702 3006
Size: 304 159 579 69 175 563 330 304
Cuts at: 3006 3914 4088 4479 4506 5851 523
Size: 908 174 391 27 1345 1001
Fragments arranged by size:
1345 1001 908 579 563 391 330 304
304 175 174 159 69 27
EagI C'GGCC_G
Cuts at: 3914 3914
Size: 6329
EarI CTCTTCn'nnn_
Cuts at: 2041 2178 2804 2821 2992 4352 4562 2041
Size: 137 626 17 171 1360 210 3808
Fragments arranged by size:
3808 1360 626 210 171 137 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 295 661 2778 4537 4847 4993 6139 295
Size: 366 2117 1759 310 146 1146 485
Fragments arranged by size:
2117 1759 1146 485 366 310 146
EcoICRI GAG'CTC
Cuts at: 600 3341 600
Size: 2741 3588
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 790 1705 2263 2464 2595 3019 3142 4154
Size: 42 915 558 201 131 424 123 1012
Cuts at: 4154 4586 5333 5601 748
Size: 432 747 268 1476
Fragments arranged by size:
1476 1012 915 747 558 432 424 268
201 131 123 42
EcoNI CCTnn'n_nnAGG
Cuts at: 505 1025 2136 2446 3208 505
Size: 520 1111 310 762 3626
Fragments arranged by size:
3626 1111 762 520 310
EcoO109I rG'GnC_Cy
Cuts at: 620 789 1254 1521 2483 2575 2640 3468 620
Size: 169 465 267 962 92 65 828 3481
Fragments arranged by size:
3481 962 828 465 267 169 92 65
EcoRI G'AATT_C
Cuts at: 2260 3333 2260
Size: 1073 5256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4348 4348
Size: 6329
FspI TGC'GCA
Cuts at: 4110 4110
Size: 6329
HaeII r_GCGC'y
Cuts at: 916 950 3763 4011 5033 6012 916
Size: 34 2813 248 1022 979 1233
Fragments arranged by size:
2813 1233 1022 979 248 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 1253 1285 2410 2442 2647 2679 2682
Size: 32 818 32 1125 32 205 32 3
Cuts at: 2682 2714 2823 2855 2896 2928 3190 3222 4171
Size: 32 109 32 41 32 262 32 949
Cuts at: 4171 4201 4203 4233 403
Size: 30 2 30 2499
Fragments arranged by size:
2499 1125 949 818 262 205 109 41
32 32 32 32 32 32 32 32
30 30 3 2
HincII GTy'rAC
Cuts at: 936 2454 3368 936
Size: 1518 914 3897
Fragments arranged by size:
3897 1518 914
KasI G'GCGC_C
Cuts at: 912 3759 4007 912
Size: 2847 248 3234
Fragments arranged by size:
3234 2847 248
KpnI G_GTAC'C
Cuts at: 1021 3349 1021
Size: 2328 4001
MfeI C'AATT_G
Cuts at: 2864 2864
Size: 6329
MluI A'CGCG_T
Cuts at: 5762 5762
Size: 6329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2821 5000 5184 2821
Size: 2179 184 3966
Fragments arranged by size:
3966 2179 184
MscI TGG'CCA
Cuts at: 525 988 1567 4090 525
Size: 463 579 2523 2764
Fragments arranged by size:
2764 2523 579 463
MslI CAynn'nnrTG
Cuts at: 709 982 1561 1817 2368 2462 2630 4318 4445
Size: 273 579 256 551 94 168 1688 127
Cuts at: 4445 709
Size: 2593
Fragments arranged by size:
2593 1688 579 551 273 256 168 127
94
MspA1I CmG'CkG
Cuts at: 133 168 449 752 807 928 1221 1605 2029
Size: 35 281 303 55 121 293 384 424
Cuts at: 2029 2122 3225 3440 3754 4114 5127 5372 133
Size: 93 1103 215 314 360 1013 245 1090
Fragments arranged by size:
1103 1090 1013 424 384 360 314 303
293 281 245 215 121 93 55 35
NaeI GCC'GGC
Cuts at: 246 849 2188 4510 246
Size: 603 1339 2322 2065
Fragments arranged by size:
2322 2065 1339 603
NarI GG'CG_CC
Cuts at: 913 3760 4008 913
Size: 2847 248 3234
Fragments arranged by size:
3234 2847 248
NcoI C'CATG_G
Cuts at: 107 2204 2648 107
Size: 2097 444 3788
Fragments arranged by size:
3788 2097 444
NdeI CA'TA_TG
Cuts at: 1629 1629
Size: 6329
NgoMIV G'CCGG_C
Cuts at: 244 847 2186 4508 244
Size: 603 1339 2322 2065
Fragments arranged by size:
2322 2065 1339 603
NsiI A_TGCA'T
Cuts at: 3312 3312
Size: 6329
NspI r_CATG'y
Cuts at: 2467 3382 3882 4789 2467
Size: 915 500 907 4007
Fragments arranged by size:
4007 915 907 500
PciI A'CATG_T
Cuts at: 2463 4785 2463
Size: 2322 4007
PflMI CCAn_nnn'nTGG
Cuts at: 3015 3015
Size: 6329
PfoI T'CCnGG_A
Cuts at: 371 725 1982 2240 371
Size: 354 1257 258 4460
Fragments arranged by size:
4460 1257 354 258
PmeI GTTT'AAAC
Cuts at: 3328 3328
Size: 6329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2235 5468 5500 2203
Size: 32 3233 32 3032
Fragments arranged by size:
3233 3032 32 32
PshAI GACnn'nnGTC
Cuts at: 1123 1123
Size: 6329
PspOMI G'GGCC_C
Cuts at: 788 1079 1254 2639 788
Size: 291 175 1385 4478
Fragments arranged by size:
4478 1385 291 175
PspXI vC'TCGA_Gb
Cuts at: 3254 3254
Size: 6329
PstI C_TGCA'G
Cuts at: 580 1456 2491 3037 3376 4061 580
Size: 876 1035 546 339 685 2848
Fragments arranged by size:
2848 1035 876 685 546 339
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6329
PvuII CAG'CTG
Cuts at: 1221 1605 2122 3754 4114 1221
Size: 384 517 1632 360 3436
Fragments arranged by size:
3436 1632 517 384 360
RsrII CG'GwC_CG
Cuts at: 4524 4524
Size: 6329
SacI G_AGCT'C
Cuts at: 602 3343 602
Size: 2741 3588
SacII CC_GC'GG
Cuts at: 134 450 929 134
Size: 316 479 5534
Fragments arranged by size:
5534 479 316
SalI G'TCGA_C
Cuts at: 934 3366 934
Size: 2432 3897
SapI GCTCTTCn'nnn_
Cuts at: 2992 4352 4562 2992
Size: 1360 210 4759
Fragments arranged by size:
4759 1360 210
SbfI CC_TGCA'GG
Cuts at: 3376 3376
Size: 6329
ScaI AGT'ACT
Cuts at: 5846 5846
Size: 6329
SexAI A'CCwGG_T
Cuts at: 2213 2213
Size: 6329
SfcI C'TryA_G
Cuts at: 33 576 717 1167 1452 2487 3033 3372 4057
Size: 543 141 450 285 1035 546 339 685
Cuts at: 4057 5050 5241 33
Size: 993 191 1121
Fragments arranged by size:
1121 1035 993 685 546 543 450 339
285 191 141
SfoI GGC'GCC
Cuts at: 914 3761 4009 914
Size: 2847 248 3234
Fragments arranged by size:
3234 2847 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6329
SgrAI Cr'CCGG_yG
Cuts at: 847 847
Size: 6329
SmaI CCC'GGG
Cuts at: 595 1068 1906 3351 595
Size: 473 838 1445 3573
Fragments arranged by size:
3573 1445 838 473
SmlI C'TyrA_G
Cuts at: 201 475 586 3254 3484 4399 4891 5153 201
Size: 274 111 2668 230 915 492 262 1377
Fragments arranged by size:
2668 1377 915 492 274 262 230 111
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6329
SphI G_CATG'C
Cuts at: 3382 3882 3382
Size: 500 5829
StuI AGG'CCT
Cuts at: 2152 2152
Size: 6329
StyI C'CwwG_G
Cuts at: 107 1517 2204 2648 2946 3463 3817 107
Size: 1410 687 444 298 517 354 2619
Fragments arranged by size:
2619 1410 687 517 444 354 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 507 1113 2124 2474 3912 4045 4504 4713 507
Size: 606 1011 350 1438 133 459 209 2123
Fragments arranged by size:
2123 1438 1011 606 459 350 209 133
TatI w'GTAC_w
Cuts at: 1424 5844 1424
Size: 4420 1909
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 552 584 1368 1400 1767 1799 1942 1974 1978
Size: 32 784 32 367 32 143 32 4
Cuts at: 1978 2010 2239 2271 5846 5878 552
Size: 32 229 32 3575 32 1003
Fragments arranged by size:
3575 1003 784 367 229 143 32 32
32 32 32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 4126 4126
Size: 6329
XbaI T'CTAG_A
Cuts at: 3360 3360
Size: 6329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 708 951 1405 2745 3824 306
Size: 402 243 454 1340 1079 2811
Fragments arranged by size:
2811 1340 1079 454 402 243
XhoI C'TCGA_G
Cuts at: 201 3254 201
Size: 3053 3276
XmaI C'CCGG_G
Cuts at: 593 1066 1904 3349 593
Size: 473 838 1445 3573
Fragments arranged by size:
3573 1445 838 473
XmnI GAAnn'nnTTC
Cuts at: 1960 1960
Size: 6329
ZraI GAC'GTC
Cuts at: 122 122
Size: 6329
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BmtI BsiWI BsrGI ClaI
DraIII EcoRV FseI FspAI HindIII HpaI NheI NotI
NruI PacI PasI PmlI PpuMI PsiI PsrI SanDI
SfiI SnaBI SrfI SspI SwaI