Restriction map for
pF1KB3507
(Circular) (Six-base) MAPSORT of: pF1KB3507.seq Check: 3905 from: 1 to: 4232
pF1KB3507 4232 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 141 141
Size: 4232
AatII G_ACGT'C
Cuts at: 1005 1005
Size: 4232
AccI GT'mk_AC
Cuts at: 517 1270 517
Size: 753 3479
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 928 2057 2489 3236 3504 858
Size: 70 1129 432 747 268 1586
Fragments arranged by size:
1586 1129 747 432 268 70
AflIII A'CryG_T
Cuts at: 158 335 1180 1584 2688 3665 158
Size: 177 845 404 1104 977 725
Fragments arranged by size:
1104 977 845 725 404 177
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AleI CACnn'nnGTG
Cuts at: 962 962
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaI G_GGCC'C
Cuts at: 578 578
Size: 4232
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 288 715 1236 3585 3841 288
Size: 427 521 2349 256 679
Fragments arranged by size:
2349 679 521 427 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 1252 1252
Size: 4232
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 539 947 1248 1662 1910 1945 539
Size: 408 301 414 248 35 2826
Fragments arranged by size:
2826 414 408 301 248 35
BanII G_rGCy'C
Cuts at: 260 578 1211 1246 2276 260
Size: 318 633 35 1030 2216
Fragments arranged by size:
2216 1030 633 318 35
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 803 1177 803
Size: 374 3858
BbvCI CC'TCA_GC
Cuts at: 117 117
Size: 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 612 646 1027 1061 612
Size: 34 381 34 3783
Fragments arranged by size:
3783 381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 542 578 1857 1950 3006 542
Size: 36 1279 93 1056 1768
Fragments arranged by size:
1768 1279 1056 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 702 1444 1525 1860 4004 702
Size: 742 81 335 2144 930
Fragments arranged by size:
2144 930 742 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 775 2599 775
Size: 1824 2408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 285 686 236
Size: 49 401 3782
Fragments arranged by size:
3782 401 49
Bpu10I CC'TnA_GC
Cuts at: 117 827 117
Size: 710 3522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 2779 3077 1408
Size: 879 492 298 2563
Fragments arranged by size:
2563 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 430 3936 430
Size: 3506 726
BsaAI yAC'GTr
Cuts at: 1181 1585 1181
Size: 404 3828
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1002 1663 1911 2613 4109 1002
Size: 661 248 702 1496 1125
Fragments arranged by size:
1496 1125 702 661 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 499
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 910 934 910
Size: 24 4208
BseYI C'CCAG_C
Cuts at: 1657 2415 2992 3488 1657
Size: 758 577 496 2401
Fragments arranged by size:
2401 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 875
Size: 4232
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1246 2024 2214 3006 1246
Size: 778 190 792 2472
Fragments arranged by size:
2472 792 778 190
BsmI GAATG_Cn'
Cuts at: 1079 1079
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 260 542 578 1211 1246 1857 1950 2024 2214
Size: 282 36 633 35 611 93 74 190
Cuts at: 2214 2276 3006 260
Size: 62 730 1486
Fragments arranged by size:
1486 730 633 611 282 190 93 74
62 36 35
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 1282 1496 1798 2179 141
Size: 1141 214 302 381 2194
Fragments arranged by size:
2194 1141 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 1198 2230 2411 2620 1198
Size: 1032 181 209 2810
Fragments arranged by size:
2810 1032 209 181
BsrGI T'GTAC_A
Cuts at: 155 155
Size: 4232
BssSI C'ACGA_G
Cuts at: 343 2503 2861 343
Size: 2160 358 1714
Fragments arranged by size:
2160 1714 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 267 267
Size: 4232
BstYI r'GATC_y
Cuts at: 1 1257 1724 1747 2082 2328 3340 3426 3438
Size: 1256 467 23 335 246 1012 86 12
Cuts at: 3438 3784 1
Size: 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 518 518
Size: 4232
Bsu36I CC'TnA_GG
Cuts at: 170 170
Size: 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1217 2182 2363 2526 1217
Size: 965 181 163 2923
Fragments arranged by size:
2923 965 181 163
BtsI GCAGTG_nn'
Cuts at: 1475 1475
Size: 4232
ClaI AT'CG_AT
Cuts at: 206 206
Size: 4232
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 1095 1817 1991 2382 2409 3754 1095
Size: 722 174 391 27 1345 1573
Fragments arranged by size:
1573 1345 722 391 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 266 2255 2465 266
Size: 1989 210 2033
Fragments arranged by size:
2033 1989 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 285 686 858 928 2057 2489 3236 3504
Size: 49 401 172 70 1129 432 747 268
Cuts at: 3504 236
Size: 964
Fragments arranged by size:
1129 964 747 432 401 268 172 70
49
EcoNI CCTnn'n_nnAGG
Cuts at: 169 621 734 169
Size: 452 113 3667
Fragments arranged by size:
3667 452 113
EcoO109I rG'GnC_Cy
Cuts at: 366 381 574 575 1371 366
Size: 15 193 1 796 3227
Fragments arranged by size:
3227 796 193 15 1
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1131 2251 1131
Size: 1120 3112
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1070 1666 1914 2936 3915 1070
Size: 596 248 1022 979 1387
Fragments arranged by size:
1387 1022 979 596 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2074 2104 2106 2136 2074
Size: 30 2 30 4170
Fragments arranged by size:
4170 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 584 1052 2903 3087 350
Size: 234 468 1851 184 1495
Fragments arranged by size:
1851 1495 468 234 184
MscI TGG'CCA
Cuts at: 1097 1993 1097
Size: 896 3336
MslI CAynn'nnrTG
Cuts at: 962 2221 2348 962
Size: 1259 127 2846
Fragments arranged by size:
2846 1259 127
MspA1I CmG'CkG
Cuts at: 1343 1657 2017 3030 3275 1343
Size: 314 360 1013 245 2300
Fragments arranged by size:
2300 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NspI r_CATG'y
Cuts at: 162 339 1285 1785 2692 162
Size: 177 946 500 907 1702
Fragments arranged by size:
1702 946 907 500 177
PasI CC'CwG_GG
Cuts at: 571 579 571
Size: 8 4224
PciI A'CATG_T
Cuts at: 158 335 2688 158
Size: 177 2353 1702
Fragments arranged by size:
2353 1702 177
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PmlI CAC'GTG
Cuts at: 1181 1181
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 366 381 366
Size: 15 4217
PspOMI G'GGCC_C
Cuts at: 574 574
Size: 4232
PstI C_TGCA'G
Cuts at: 435 1279 1964 435
Size: 844 685 2703
Fragments arranged by size:
2703 844 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3872
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SanDI GG'GwC_CC
Cuts at: 366 366
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 306 3749 306
Size: 3443 789
SfcI C'TryA_G
Cuts at: 33 431 1275 1960 2953 3144 33
Size: 398 844 685 993 191 1121
Fragments arranged by size:
1121 993 844 685 398 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 1387 2302 2794 3056 1387
Size: 915 492 262 2563
Fragments arranged by size:
2563 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StuI AGG'CCT
Cuts at: 429 986 429
Size: 557 3675
StyI C'CwwG_G
Cuts at: 881 977 1366 1720 881
Size: 96 389 354 3393
Fragments arranged by size:
3393 389 354 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 753 1815 1948 2407 2616 753
Size: 1062 133 459 209 2369
Fragments arranged by size:
2369 1062 459 209 133
TatI w'GTAC_w
Cuts at: 155 304 416 911 1217 3747 155
Size: 149 112 495 306 2530 640
Fragments arranged by size:
2530 640 495 306 149 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 984 1727 984
Size: 743 3489
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
ZraI GAC'GTC
Cuts at: 1003 1003
Size: 4232
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BtgI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI