Restriction map for
pF1KB3508
(Circular) (Six-base) MAPSORT of: pF1KB3508.seq Check: 1627 from: 1 to: 4097
pF1KB3508 4097 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 999 1045 1922 2354 3101 3369 948
Size: 51 46 877 432 747 268 1676
Fragments arranged by size:
1676 877 747 432 268 51 46
AflIII A'CryG_T
Cuts at: 417 1449 2553 3530 417
Size: 1032 1104 977 984
Fragments arranged by size:
1104 1032 984 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 221 253 221
Size: 32 4065
AlwNI CAG_nnn'CTG
Cuts at: 622 1402 2969 622
Size: 780 1567 1750
Fragments arranged by size:
1750 1567 780
ApaI G_GGCC'C
Cuts at: 470 470
Size: 4097
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 177 1101 3450 3706 177
Size: 924 2349 256 568
Fragments arranged by size:
2349 924 568 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 1117 1117
Size: 4097
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 1113 1527 1775 1810 1113
Size: 414 248 35 3400
Fragments arranged by size:
3400 414 248 35
BanII G_rGCy'C
Cuts at: 470 1053 1111 2141 470
Size: 583 58 1030 2426
Fragments arranged by size:
2426 1030 583 58
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 420 420
Size: 4097
BciVI GTATCCnnnnn_n'
Cuts at: 432 1995 2756 4097 432
Size: 1563 761 1341 432
Fragments arranged by size:
1563 1341 761 432
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BfrBI ATG'CAT
Cuts at: 748 900 748
Size: 152 3945
BglII A'GATC_T
Cuts at: 938 1612 3649 938
Size: 674 2037 1386
Fragments arranged by size:
2037 1386 674
BlpI GC'TnA_GC
Cuts at: 916 1209 916
Size: 293 3804
Bme1580I G_kGCm'C
Cuts at: 470 1722 1815 2871 470
Size: 1252 93 1056 1696
Fragments arranged by size:
1696 1252 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 511 482
Size: 29 4068
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1273 2152 2644 2942 1072
Size: 201 879 492 298 2227
Fragments arranged by size:
2227 879 492 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 316 784 3801 316
Size: 468 3017 612
Fragments arranged by size:
3017 612 468
BsaAI yAC'GTr
Cuts at: 420 1450 420
Size: 1030 3067
BsaBI GATnn'nnATC
Cuts at: 6 826 1611 3648 6
Size: 820 785 2037 455
Fragments arranged by size:
2037 820 785 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 535 1075 508
Size: 27 540 3530
Fragments arranged by size:
3530 540 27
BseYI C'CCAG_C
Cuts at: 1522 2280 2857 3353 1522
Size: 758 577 496 2266
Fragments arranged by size:
2266 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 446 542 1111 1889 2079 2871 446
Size: 96 569 778 190 792 1672
Fragments arranged by size:
1672 792 778 569 190 96
BsmI GAATG_Cn'
Cuts at: 707 900 707
Size: 193 3904
Bsp1286I G_dGCh'C
Cuts at: 446 470 542 1053 1111 1722 1815 1889 2079
Size: 24 72 511 58 611 93 74 190
Cuts at: 2079 2141 2871 446
Size: 62 730 1672
Fragments arranged by size:
1672 730 611 511 190 93 74 72
62 58 24
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1361 1663 2044 1147
Size: 214 302 381 3200
Fragments arranged by size:
3200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 485 2095 2276 2485 485
Size: 1610 181 209 2097
Fragments arranged by size:
2097 1610 209 181
BsrGI T'GTAC_A
Cuts at: 401 401
Size: 4097
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 906 1474 906
Size: 568 3529
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstYI r'GATC_y
Cuts at: 1 497 938 1122 1589 1612 1947 2193 3205
Size: 496 441 184 467 23 335 246 1012
Cuts at: 3205 3291 3303 3649 1
Size: 86 12 346 449
Fragments arranged by size:
1012 496 467 449 441 346 335 246
184 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 1682 1856 2247 2274 3619 1682
Size: 174 391 27 1345 2160
Fragments arranged by size:
2160 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 365 2120 2330 365
Size: 1755 210 2132
Fragments arranged by size:
2132 1755 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 511 948 999 1045 1922 2354 3101 3369
Size: 29 437 51 46 877 432 747 268
Cuts at: 3369 482
Size: 1210
Fragments arranged by size:
1210 877 747 437 432 268 51 46
29
EcoNI CCTnn'n_nnAGG
Cuts at: 436 436
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 466 1236 466
Size: 770 3327
EcoRI G'AATT_C
Cuts at: 177 1101 177
Size: 924 3173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 462 2116 462
Size: 1654 2443
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 1531 1779 2801 3780 1531
Size: 248 1022 979 1848
Fragments arranged by size:
1848 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 704 736 1939 1969 1971 2001 704
Size: 32 1203 30 2 30 2800
Fragments arranged by size:
2800 1203 32 30 30 2
HincII GTy'rAC
Cuts at: 315 453 1136 315
Size: 138 683 3276
Fragments arranged by size:
3276 683 138
HindIII A'AGCT_T
Cuts at: 231 1057 231
Size: 826 3271
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 515 525 835 2768 2952 515
Size: 10 310 1933 184 1660
Fragments arranged by size:
1933 1660 310 184 10
MscI TGG'CCA
Cuts at: 1858 1858
Size: 4097
MslI CAynn'nnrTG
Cuts at: 2086 2213 2086
Size: 127 3970
MspA1I CmG'CkG
Cuts at: 992 1208 1522 1882 2895 3140 992
Size: 216 314 360 1013 245 1949
Fragments arranged by size:
1949 1013 360 314 245 216
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 107 107
Size: 4097
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NsiI A_TGCA'T
Cuts at: 750 902 750
Size: 152 3945
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 489 489
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PsiI TTA'TAA
Cuts at: 664 664
Size: 4097
PspOMI G'GGCC_C
Cuts at: 466 466
Size: 4097
PstI C_TGCA'G
Cuts at: 1144 1829 1144
Size: 685 3412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 1522 1882 1522
Size: 360 3737
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 757 1140 1825 2818 3009 33
Size: 724 383 685 993 191 1121
Fragments arranged by size:
1121 993 724 685 383 191
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 1087 1252 2167 2659 2921 1087
Size: 165 915 492 262 2263
Fragments arranged by size:
2263 915 492 262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StuI AGG'CCT
Cuts at: 559 969 559
Size: 410 3687
StyI C'CwwG_G
Cuts at: 107 528 1231 1585 107
Size: 421 703 354 2619
Fragments arranged by size:
2619 703 421 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 1813 2272 2481 1680
Size: 133 459 209 3296
Fragments arranged by size:
3296 459 209 133
TatI w'GTAC_w
Cuts at: 401 3612 401
Size: 3211 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 173 205 375 407 3614 3646 173
Size: 32 170 32 3207 32 624
Fragments arranged by size:
3207 624 170 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI