Restriction map for
pF1KB3511
(Circular) (Six-base) MAPSORT of: pF1KB3511.seq Check: 324 from: 1 to: 4514
pF1KB3511 4514 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 998 998
Size: 4514
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2339 2771 3124 3786 1435
Size: 904 432 353 662 2163
Fragments arranged by size:
2163 904 662 432 353
AflIII A'CryG_T
Cuts at: 1866 3666 3947 1866
Size: 1800 281 2433
Fragments arranged by size:
2433 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1501 1501
Size: 4514
AlwNI CAG_nnn'CTG
Cuts at: 499 943 972 1819 3257 499
Size: 444 29 847 1438 1756
Fragments arranged by size:
1756 1438 847 444 29
ApaLI G'TGCA_C
Cuts at: 3352 3352
Size: 4514
ApoI r'AATT_y
Cuts at: 382 931 1518 3867 4123 382
Size: 549 587 2349 256 773
Fragments arranged by size:
2349 773 587 549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 1534 1534
Size: 4514
BamHI G'GATC_C
Cuts at: 487 1539 487
Size: 1052 3462
BanI G'GyrC_C
Cuts at: 403 414 627 720 1530 1944 2192 2227 403
Size: 11 213 93 810 414 248 35 2690
Fragments arranged by size:
2690 810 414 248 213 93 35 11
BanII G_rGCy'C
Cuts at: 185 472 902 1528 2558 185
Size: 287 430 626 1030 2141
Fragments arranged by size:
2141 1030 626 430 287
BbeI G_GCGC'C
Cuts at: 631 1948 2196 631
Size: 1317 248 2949
Fragments arranged by size:
2949 1317 248
BbvCI CC'TCA_GC
Cuts at: 458 458
Size: 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1156 1190 1156
Size: 34 4480
BciVI GTATCCnnnnn_n'
Cuts at: 2412 3468 4514 2412
Size: 1056 1046 2412
Fragments arranged by size:
2412 1056 1046
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BglII A'GATC_T
Cuts at: 1217 1373 2029 4066 1217
Size: 156 656 2037 1665
Fragments arranged by size:
2037 1665 656 156
BlpI GC'TnA_GC
Cuts at: 1409 1626 1409
Size: 217 4297
Bme1580I G_kGCm'C
Cuts at: 419 1092 2139 2232 3356 419
Size: 673 1047 93 1124 1577
Fragments arranged by size:
1577 1124 1047 673 93
BmrI ACTGGGnnnn_n'
Cuts at: 421 1726 1807 2142 4286 421
Size: 1305 81 335 2144 649
Fragments arranged by size:
2144 1305 649 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 860 2881 860
Size: 2021 2493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 731 940 1039 721
Size: 10 209 99 4196
Fragments arranged by size:
4196 209 99 10
Bpu10I CC'TnA_GC
Cuts at: 458 944 1162 458
Size: 486 218 3810
Fragments arranged by size:
3810 486 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2569 3283 3581 1690
Size: 879 714 298 2623
Fragments arranged by size:
2623 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 4514
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 628 995 1945 2193 2895 4391 628
Size: 367 950 248 702 1496 751
Fragments arranged by size:
1496 950 751 702 367 248
BsaWI w'CCGG_w
Cuts at: 1703 2224 2902 3313 3460 3734 1703
Size: 521 678 411 147 274 2483
Fragments arranged by size:
2483 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 664 694 664
Size: 30 4484
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 910 910
Size: 4514
BseYI C'CCAG_C
Cuts at: 333 350 1474 1939 2697 3362 3770 333
Size: 17 1124 465 758 665 408 1077
Fragments arranged by size:
1124 1077 758 665 465 408 17
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3332 104
Size: 1952 46 1230 1286
Fragments arranged by size:
1952 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 366 902 1528 2306 2496 3356 366
Size: 536 626 778 190 860 1524
Fragments arranged by size:
1524 860 778 626 536 190
Bsp1286I G_dGCh'C
Cuts at: 185 366 419 472 902 1092 1528 2139 2232
Size: 181 53 53 430 190 436 611 93
Cuts at: 2232 2306 2496 2558 3356 185
Size: 74 190 62 798 1343
Fragments arranged by size:
1343 798 611 436 430 190 190 181
93 74 62 53 53
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 915 1564 1778 2080 2461 915
Size: 649 214 302 381 2968
Fragments arranged by size:
2968 649 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1172 2426 1172
Size: 1254 3260
BsrFI r'CCGG_y
Cuts at: 2512 2693 2512
Size: 181 4333
BssSI C'ACGA_G
Cuts at: 2785 3493 2785
Size: 708 3806
BstAPI GCAn_nnn'nTGC
Cuts at: 1461 1891 1461
Size: 430 4084
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 341 755 341
Size: 414 4100
BstYI r'GATC_y
Cuts at: 1 487 1217 1373 1539 2006 2029 2364 2610
Size: 486 730 156 166 467 23 335 246
Cuts at: 2610 2916 2928 3014 4066 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 730 486 467 449 335 306 246
166 156 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 845 845
Size: 4514
BtgI C'CryG_G
Cuts at: 107 623 804 107
Size: 516 181 3817
Fragments arranged by size:
3817 516 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 657 683 1365 1757 657
Size: 26 682 392 3414
Fragments arranged by size:
3414 682 392 26
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3564 2220
Size: 1344 3170
EaeI y'GGCC_r
Cuts at: 1243 2099 2273 2664 2691 4036 1243
Size: 856 174 391 27 1345 1721
Fragments arranged by size:
1721 1345 856 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 254 865 2537 2747 254
Size: 611 1672 210 2021
Fragments arranged by size:
2021 1672 611 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1354 2722 3464 3610 4324 1354
Size: 1368 742 146 714 1544
Fragments arranged by size:
1544 1368 742 714 146
EcoICRI GAG'CTC
Cuts at: 900 1526 900
Size: 626 3888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 731 940 1039 1435 2339 2771 3124 3786
Size: 10 209 99 396 904 432 353 662
Cuts at: 3786 721
Size: 1449
Fragments arranged by size:
1449 904 662 432 396 353 209 99
10
EcoNI CCTnn'n_nnAGG
Cuts at: 922 922
Size: 4514
EcoO109I rG'GnC_Cy
Cuts at: 870 877 940 1653 870
Size: 7 63 713 3731
Fragments arranged by size:
3731 713 63 7
EcoRI G'AATT_C
Cuts at: 382 1518 382
Size: 1136 3378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 631 1948 2196 3426 4197 631
Size: 1317 248 1230 771 948
Fragments arranged by size:
1317 1230 948 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 750 782 998 1030 2356 2386 2388 2418 750
Size: 32 216 32 1326 30 2 30 2846
Fragments arranged by size:
2846 1326 216 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1553 1553
Size: 4514
KasI G'GCGC_C
Cuts at: 627 1944 2192 627
Size: 1317 248 2949
Fragments arranged by size:
2949 1317 248
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MfeI C'AATT_G
Cuts at: 645 645
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3273 3457 3273
Size: 184 4330
MscI TGG'CCA
Cuts at: 1245 2275 1245
Size: 1030 3484
MslI CAynn'nnrTG
Cuts at: 832 2503 2630 832
Size: 1671 127 2716
Fragments arranged by size:
2716 1671 127
MspA1I CmG'CkG
Cuts at: 354 1028 1413 1422 1625 1939 2299 3083 3328
Size: 674 385 9 203 314 360 784 245
Cuts at: 3328 354
Size: 1540
Fragments arranged by size:
1540 784 674 385 360 314 245 203
9
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 628 1945 2193 628
Size: 1317 248 2949
Fragments arranged by size:
2949 1317 248
NcoI C'CATG_G
Cuts at: 107 623 107
Size: 516 3998
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NspI r_CATG'y
Cuts at: 235 1482 1567 2067 3670 235
Size: 1247 85 500 1603 1079
Fragments arranged by size:
1603 1247 1079 500 85
PasI CC'CwG_GG
Cuts at: 178 465 506 178
Size: 287 41 4186
Fragments arranged by size:
4186 287 41
PciI A'CATG_T
Cuts at: 3666 3666
Size: 4514
PfoI T'CCnGG_A
Cuts at: 584 1016 584
Size: 432 4082
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 4482
PpuMI rG'GwC_Cy
Cuts at: 870 877 940 870
Size: 7 63 4444
Fragments arranged by size:
4444 63 7
PshAI GACnn'nnGTC
Cuts at: 875 875
Size: 4514
PstI C_TGCA'G
Cuts at: 667 1373 1561 2246 667
Size: 706 188 685 2935
Fragments arranged by size:
2935 706 685 188
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 354 1413 1422 1939 2299 354
Size: 1059 9 517 360 2569
Fragments arranged by size:
2569 1059 517 360 9
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 902 1528 902
Size: 626 3888
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SanDI GG'GwC_CC
Cuts at: 877 877
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 2537 2747 2537
Size: 210 4304
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SfcI C'TryA_G
Cuts at: 33 290 663 738 1369 1557 2242 3210 3401
Size: 257 373 75 631 188 685 968 191
Cuts at: 3401 33
Size: 1146
Fragments arranged by size:
1146 968 685 631 373 257 191 188
75
SfoI GGC'GCC
Cuts at: 629 1946 2194 629
Size: 1317 248 2949
Fragments arranged by size:
2949 1317 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1536 1536
Size: 4514
SmlI C'TyrA_G
Cuts at: 1669 2584 3298 3560 1669
Size: 915 714 262 2623
Fragments arranged by size:
2623 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 1482 1567 2067 1482
Size: 85 500 3929
Fragments arranged by size:
3929 500 85
StuI AGG'CCT
Cuts at: 283 283
Size: 4514
StyI C'CwwG_G
Cuts at: 107 407 623 1648 2002 107
Size: 300 216 1025 354 2619
Fragments arranged by size:
2619 1025 354 300 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 1070 4029 1070
Size: 2959 1555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 664 696 4031 4063 664
Size: 32 3335 32 1115
Fragments arranged by size:
3335 1115 32 32
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 163 2009 163
Size: 1846 2668
XmaI C'CCGG_G
Cuts at: 1534 1534
Size: 4514
XmnI GAAnn'nnTTC
Cuts at: 973 973
Size: 4514
ZraI GAC'GTC
Cuts at: 996 996
Size: 4514
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI