Restriction map for
pF1KB3513
(Circular) (Six-base) MAPSORT of: pF1KB3513.seq Check: 466 from: 1 to: 6182
pF1KB3513 6182 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2866 2866
Size: 6182
AccI GT'mk_AC
Cuts at: 884 3220 884
Size: 2336 3846
Acc65I G'GTAC_C
Cuts at: 3198 3198
Size: 6182
AclI AA'CG_TT
Cuts at: 6022 6022
Size: 6182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1334 1826 4007 4439 4792 5454 232
Size: 1102 492 2181 432 353 662 960
Fragments arranged by size:
2181 1102 960 662 492 432 353
AflII C'TTAA_G
Cuts at: 1435 1435
Size: 6182
AflIII A'CryG_T
Cuts at: 1277 3534 5334 5615 1277
Size: 2257 1800 281 1844
Fragments arranged by size:
2257 1844 1800 281
AleI CACnn'nnGTG
Cuts at: 997 2108 997
Size: 1111 5071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 753 753
Size: 6182
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 965 997 965
Size: 32 6150
AlwNI CAG_nnn'CTG
Cuts at: 1695 2694 3487 4925 1695
Size: 999 793 1438 2952
Fragments arranged by size:
2952 1438 999 793
ApaLI G'TGCA_C
Cuts at: 5020 5020
Size: 6182
ApoI r'AATT_y
Cuts at: 701 1836 2931 3186 5535 5791 701
Size: 1135 1095 255 2349 256 1092
Fragments arranged by size:
2349 1135 1095 1092 256 255
AseI AT'TA_AT
Cuts at: 20 20
Size: 6182
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
AvaI C'yCGr_G
Cuts at: 2461 3202 2461
Size: 741 5441
AvrII C'CTAG_G
Cuts at: 1764 1764
Size: 6182
BamHI G'GATC_C
Cuts at: 1646 3207 1646
Size: 1561 4621
BanI G'GyrC_C
Cuts at: 862 1633 3198 3612 3860 3895 862
Size: 771 1565 414 248 35 3149
Fragments arranged by size:
3149 1565 771 414 248 35
BanII G_rGCy'C
Cuts at: 1610 1871 3196 4226 1610
Size: 261 1325 1030 3566
Fragments arranged by size:
3566 1325 1030 261
BbeI G_GCGC'C
Cuts at: 1637 3616 3864 1637
Size: 1979 248 3955
Fragments arranged by size:
3955 1979 248
BbsI GAAGACnn'nnnn_
Cuts at: 2039 2810 2931 2039
Size: 771 121 5290
Fragments arranged by size:
5290 771 121
BbvCI CC'TCA_GC
Cuts at: 605 2192 605
Size: 1587 4595
BciVI GTATCCnnnnn_n'
Cuts at: 3059 4080 5136 6182 3059
Size: 1021 1056 1046 3059
Fragments arranged by size:
3059 1056 1046 1021
BclI T'GATC_A
Cuts at: 234 2654 3702 234
Size: 2420 1048 2714
Fragments arranged by size:
2714 2420 1048
BglI GCCn_nnn'nGGC
Cuts at: 3149 3149
Size: 6182
BglII A'GATC_T
Cuts at: 2963 3697 5734 2963
Size: 734 2037 3411
Fragments arranged by size:
3411 2037 734
BlpI GC'TnA_GC
Cuts at: 3294 3294
Size: 6182
Bme1580I G_kGCm'C
Cuts at: 2548 3807 3900 5024 2548
Size: 1259 93 1124 3706
Fragments arranged by size:
3706 1259 1124 93
BmgBI CAC'GTC
Cuts at: 127 1217 127
Size: 1090 5092
BmrI ACTGGGnnnn_n'
Cuts at: 899 2053 2521 2784 3394 3475 3810 5954 899
Size: 1154 468 263 610 81 335 2144 1127
Fragments arranged by size:
2144 1154 1127 610 468 335 263 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4549 4549
Size: 6182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 838 2258 2408 776
Size: 62 1420 150 4550
Fragments arranged by size:
4550 1420 150 62
Bpu10I CC'TnA_GC
Cuts at: 605 2192 605
Size: 1587 4595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 1066 1081 3358 4237 4951 5249 303
Size: 763 15 2277 879 714 298 1236
Fragments arranged by size:
2277 1236 879 763 714 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 689 794 1195 1955 2017 5886 689
Size: 105 401 760 62 3869 985
Fragments arranged by size:
3869 985 760 401 105 62
BsaAI yAC'GTr
Cuts at: 871 3535 871
Size: 2664 3518
BsaBI GATnn'nnATC
Cuts at: 6 297 3696 5733 6
Size: 291 3399 2037 455
Fragments arranged by size:
3399 2037 455 291
BsaHI Gr'CG_yC
Cuts at: 1634 1715 3141 3613 3861 4563 6059 1634
Size: 81 1426 472 248 702 1496 1757
Fragments arranged by size:
1757 1496 1426 702 472 248 81
BsaWI w'CCGG_w
Cuts at: 1259 3371 3892 4570 4981 5128 5402 1259
Size: 2112 521 678 411 147 274 2039
Fragments arranged by size:
2112 2039 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 339 369 774 804 1745 1775 2113 2143 339
Size: 30 405 30 941 30 338 30 4378
Fragments arranged by size:
4378 941 405 338 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 707 744 1663 1717 707
Size: 37 919 54 5172
Fragments arranged by size:
5172 919 54 37
BseYI C'CCAG_C
Cuts at: 1740 3152 3607 4365 5030 5438 1740
Size: 1412 455 758 665 408 2484
Fragments arranged by size:
2484 1412 758 665 455 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 2914 329
Size: 2585 3597
BsiEI CG_ry'CG
Cuts at: 104 1901 3724 3770 5000 104
Size: 1797 1823 46 1230 1286
Fragments arranged by size:
1823 1797 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3196 3974 4164 5024 3196
Size: 778 190 860 4354
Fragments arranged by size:
4354 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 814 814
Size: 6182
Bsp1286I G_dGCh'C
Cuts at: 1610 1871 2548 3196 3807 3900 3974 4164 4226
Size: 261 677 648 611 93 74 190 62
Cuts at: 4226 5024 1610
Size: 798 2768
Fragments arranged by size:
2768 798 677 648 611 261 190 93
74 62
BspHI T'CATG_A
Cuts at: 2600 4614 2600
Size: 2014 4168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1372 2866 3232 3446 3748 4129 1372
Size: 1494 366 214 302 381 3425
Fragments arranged by size:
3425 1494 381 366 302 214
BsrBI CCG'CTC
Cuts at: 50 2649 4474 4528 6012 6177 50
Size: 2599 1825 54 1484 165 55
Fragments arranged by size:
2599 1825 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 830 956 2025 2036 2432 4094 830
Size: 126 1069 11 396 1662 2918
Fragments arranged by size:
2918 1662 1069 396 126 11
BsrFI r'CCGG_y
Cuts at: 4180 4361 4180
Size: 181 6001
BssSI C'ACGA_G
Cuts at: 305 4453 5161 305
Size: 4148 708 1326
Fragments arranged by size:
4148 1326 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1456 3559 1456
Size: 2103 4079
BstBI TT'CG_AA
Cuts at: 624 4543 624
Size: 3919 2263
BstXI CCAn_nnnn'nTGG
Cuts at: 1158 1998 2571 1158
Size: 840 573 4769
Fragments arranged by size:
4769 840 573
BstYI r'GATC_y
Cuts at: 1 317 672 1646 2963 3207 3674 3697 4032
Size: 316 355 974 1317 244 467 23 335
Cuts at: 4032 4278 4584 4596 4682 5734 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1317 1052 974 467 449 355 335 316
306 246 244 86 23 12
BtgI C'CryG_G
Cuts at: 107 1677 1891 1913 2027 107
Size: 1570 214 22 114 4262
Fragments arranged by size:
4262 1570 214 114 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4132 4313 4476 4132
Size: 181 163 5838
Fragments arranged by size:
5838 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 2115 2827 2902 3425 1406
Size: 709 712 75 523 4163
Fragments arranged by size:
4163 712 709 523 75
ClaI AT'CG_AT
Cuts at: 1847 1847
Size: 6182
DraI TTT'AAA
Cuts at: 3181 3181
Size: 6182
DrdI GACnn_nn'nnGTC
Cuts at: 1209 3888 5232 1209
Size: 2679 1344 2159
Fragments arranged by size:
2679 2159 1344
EaeI y'GGCC_r
Cuts at: 1118 1898 3767 3941 4332 4359 5704 1118
Size: 780 1869 174 391 27 1345 1596
Fragments arranged by size:
1869 1596 1345 780 391 174 27
EagI C'GGCC_G
Cuts at: 1898 3767 1898
Size: 1869 4313
EarI CTCTTCn'nnn_
Cuts at: 467 2599 2981 3098 4205 4415 467
Size: 2132 382 117 1107 210 2234
Fragments arranged by size:
2234 2132 1107 382 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4390 5132 5278 5992 4390
Size: 742 146 714 4580
Fragments arranged by size:
4580 742 714 146
EcoICRI GAG'CTC
Cuts at: 3194 3194
Size: 6182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 776 838 1334 1826 2258 2408 4007 4439
Size: 544 62 496 492 432 150 1599 432
Cuts at: 4439 4792 5454 232
Size: 353 662 960
Fragments arranged by size:
1599 960 662 544 496 492 432 432
353 150 62
EcoNI CCTnn'n_nnAGG
Cuts at: 1699 1699
Size: 6182
EcoO109I rG'GnC_Cy
Cuts at: 1746 2183 2691 3321 1746
Size: 437 508 630 4607
Fragments arranged by size:
4607 630 508 437
EcoRI G'AATT_C
Cuts at: 701 2931 3186 701
Size: 2230 255 3697
Fragments arranged by size:
3697 2230 255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4201 4201
Size: 6182
FspI TGC'GCA
Cuts at: 3963 3963
Size: 6182
HaeII r_GCGC'y
Cuts at: 1637 3616 3864 5094 5865 1637
Size: 1979 248 1230 771 1954
Fragments arranged by size:
1979 1954 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 739 771 774 806 1305 1337 1745 1777 1872
Size: 32 3 32 499 32 408 32 95
Cuts at: 1872 1904 2113 2145 2175 2207 4024 4054 4056
Size: 32 209 32 30 32 1817 30 2
Cuts at: 4056 4086 739
Size: 30 2835
Fragments arranged by size:
2835 1817 499 408 209 95 32 32
32 32 32 32 32 30 30 30
3 2
HincII GTy'rAC
Cuts at: 3221 3221
Size: 6182
KasI G'GCGC_C
Cuts at: 1633 3612 3860 1633
Size: 1979 248 3955
Fragments arranged by size:
3955 1979 248
KpnI G_GTAC'C
Cuts at: 3202 3202
Size: 6182
MfeI C'AATT_G
Cuts at: 849 2378 849
Size: 1529 4653
MluI A'CGCG_T
Cuts at: 5615 5615
Size: 6182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 102 4941 5125 102
Size: 4839 184 1159
Fragments arranged by size:
4839 1159 184
MscI TGG'CCA
Cuts at: 1120 3943 1120
Size: 2823 3359
MslI CAynn'nnrTG
Cuts at: 904 997 1876 2108 2209 2551 4171 4298 904
Size: 93 879 232 101 342 1620 127 2788
Fragments arranged by size:
2788 1620 879 342 232 127 101 93
MspA1I CmG'CkG
Cuts at: 148 313 2139 2786 3293 3607 3967 4751 4996
Size: 165 1826 647 507 314 360 784 245
Cuts at: 4996 148
Size: 1334
Fragments arranged by size:
1826 1334 784 647 507 360 314 245
165
NaeI GCC'GGC
Cuts at: 4363 4363
Size: 6182
NarI GG'CG_CC
Cuts at: 1634 3613 3861 1634
Size: 1979 248 3955
Fragments arranged by size:
3955 1979 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6182
NgoMIV G'CCGG_C
Cuts at: 4361 4361
Size: 6182
NspI r_CATG'y
Cuts at: 1281 3235 3735 5338 1281
Size: 1954 500 1603 2125
Fragments arranged by size:
2125 1954 1603 500
PasI CC'CwG_GG
Cuts at: 2078 2694 2078
Size: 616 5566
PciI A'CATG_T
Cuts at: 1277 5334 1277
Size: 4057 2125
PflMI CCAn_nnn'nTGG
Cuts at: 2694 2694
Size: 6182
PmeI GTTT'AAAC
Cuts at: 3181 3181
Size: 6182
PmlI CAC'GTG
Cuts at: 871 871
Size: 6182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1236 1268 4628 4660 1236
Size: 32 3360 32 2758
Fragments arranged by size:
3360 2758 32 32
PpuMI rG'GwC_Cy
Cuts at: 2183 2183
Size: 6182
PstI C_TGCA'G
Cuts at: 147 966 3229 3914 147
Size: 819 2263 685 2415
Fragments arranged by size:
2415 2263 819 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6182
PvuII CAG'CTG
Cuts at: 148 313 2139 3607 3967 148
Size: 165 1826 1468 360 2363
Fragments arranged by size:
2363 1826 1468 360 165
RsrII CG'GwC_CG
Cuts at: 4377 4377
Size: 6182
SacI G_AGCT'C
Cuts at: 3196 3196
Size: 6182
SalI G'TCGA_C
Cuts at: 3219 3219
Size: 6182
SapI GCTCTTCn'nnn_
Cuts at: 4205 4415 4205
Size: 210 5972
SbfI CC_TGCA'GG
Cuts at: 3229 3229
Size: 6182
ScaI AGT'ACT
Cuts at: 5699 5699
Size: 6182
SexAI A'CCwGG_T
Cuts at: 3064 3064
Size: 6182
SfcI C'TryA_G
Cuts at: 33 143 467 962 3225 3910 4878 5069 33
Size: 110 324 495 2263 685 968 191 1146
Fragments arranged by size:
2263 1146 968 685 495 324 191 110
SfoI GGC'GCC
Cuts at: 1635 3614 3862 1635
Size: 1979 248 3955
Fragments arranged by size:
3955 1979 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
SmaI CCC'GGG
Cuts at: 3204 3204
Size: 6182
SmlI C'TyrA_G
Cuts at: 282 1045 1060 1435 3337 4252 4966 5228 282
Size: 763 15 375 1902 915 714 262 1236
Fragments arranged by size:
1902 1236 915 763 714 375 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6182
SphI G_CATG'C
Cuts at: 3235 3735 3235
Size: 500 5682
StuI AGG'CCT
Cuts at: 2092 2092
Size: 6182
StyI C'CwwG_G
Cuts at: 107 1205 1764 2087 3316 3670 107
Size: 1098 559 323 1229 354 2619
Fragments arranged by size:
2619 1229 1098 559 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 493 713 1003 2925 3019 3144 3765 3898 4357
Size: 220 290 1922 94 125 621 133 459
Cuts at: 4357 4566 493
Size: 209 2109
Fragments arranged by size:
2109 1922 621 459 290 220 209 133
125 94
TatI w'GTAC_w
Cuts at: 1049 2100 5697 1049
Size: 1051 3597 1534
Fragments arranged by size:
3597 1534 1051
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 339 371 5699 5731 339
Size: 32 5328 32 790
Fragments arranged by size:
5328 790 32 32
Tth111I GACn'n_nGTC
Cuts at: 786 2908 3115 3979 786
Size: 2122 207 864 2989
Fragments arranged by size:
2989 2122 864 207
XbaI T'CTAG_A
Cuts at: 3213 3213
Size: 6182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3677 3677
Size: 6182
XmaI C'CCGG_G
Cuts at: 3202 3202
Size: 6182
XmnI GAAnn'nnTTC
Cuts at: 1799 2737 1799
Size: 938 5244
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI ApaI AscI BaeI BcgI
BfrBI BmtI BsiWI BsmI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI