Restriction map for
pF1KB3518
(Circular) (Six-base) MAPSORT of: pF1KB3518.seq Check: 8853 from: 1 to: 5393
pF1KB3518 5393 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2185 2185
Size: 5393
AatII G_ACGT'C
Cuts at: 1286 1286
Size: 5393
AccI GT'mk_AC
Cuts at: 2431 2431
Size: 5393
Acc65I G'GTAC_C
Cuts at: 2409 2409
Size: 5393
AclI AA'CG_TT
Cuts at: 5233 5233
Size: 5393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 502 532 1767 3218 3650 4003 4665 391
Size: 111 30 1235 1451 432 353 662 1119
Fragments arranged by size:
1451 1235 1119 662 432 353 111 30
AfeI AGC'GCT
Cuts at: 874 874
Size: 5393
AflIII A'CryG_T
Cuts at: 2745 4545 4826 2745
Size: 1800 281 3312
Fragments arranged by size:
3312 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 690 690
Size: 5393
AleI CACnn'nnGTG
Cuts at: 1912 1912
Size: 5393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1683 2193 1683
Size: 510 4883
AlwNI CAG_nnn'CTG
Cuts at: 651 784 1803 2698 4136 651
Size: 133 1019 895 1438 1908
Fragments arranged by size:
1908 1438 1019 895 133
ApaI G_GGCC'C
Cuts at: 184 235 275 286 765 1606 184
Size: 51 40 11 479 841 3971
Fragments arranged by size:
3971 841 479 51 40 11
ApaLI G'TGCA_C
Cuts at: 1116 2143 4231 1116
Size: 1027 2088 2278
Fragments arranged by size:
2278 2088 1027
ApoI r'AATT_y
Cuts at: 2397 4746 5002 2397
Size: 2349 256 2788
Fragments arranged by size:
2788 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5393
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
AvaI C'yCGr_G
Cuts at: 223 268 2021 2413 223
Size: 45 1753 392 3203
Fragments arranged by size:
3203 1753 392 45
BamHI G'GATC_C
Cuts at: 308 1704 2418 308
Size: 1396 714 3283
Fragments arranged by size:
3283 1396 714
BanI G'GyrC_C
Cuts at: 1005 1825 2409 2823 3071 3106 1005
Size: 820 584 414 248 35 3292
Fragments arranged by size:
3292 820 584 414 248 35
BanII G_rGCy'C
Cuts at: 184 235 275 286 293 734 765 774 794
Size: 51 40 11 7 441 31 9 20
Cuts at: 794 1078 1101 1135 1596 1606 2407 3437 184
Size: 284 23 34 461 10 801 1030 2140
Fragments arranged by size:
2140 1030 801 461 441 284 51 40
34 31 23 20 11 10 9 7
BbeI G_GCGC'C
Cuts at: 2827 3075 2827
Size: 248 5145
BbsI GAAGACnn'nnnn_
Cuts at: 935 1025 1040 1278 1411 2373 935
Size: 90 15 238 133 962 3955
Fragments arranged by size:
3955 962 238 133 90 15
BbvCI CC'TCA_GC
Cuts at: 1366 1696 1366
Size: 330 5063
BciVI GTATCCnnnnn_n'
Cuts at: 3291 4347 5393 3291
Size: 1056 1046 3291
Fragments arranged by size:
3291 1056 1046
BclI T'GATC_A
Cuts at: 2913 2913
Size: 5393
BglI GCCn_nnn'nGGC
Cuts at: 769 769
Size: 5393
BglII A'GATC_T
Cuts at: 1338 2908 4945 1338
Size: 1570 2037 1786
Fragments arranged by size:
2037 1786 1570
BlpI GC'TnA_GC
Cuts at: 1735 2505 1735
Size: 770 4623
Bme1580I G_kGCm'C
Cuts at: 184 235 275 286 765 1010 1120 1606 2004
Size: 51 40 11 479 245 110 486 398
Cuts at: 2004 2147 3018 3111 4235 184
Size: 143 871 93 1124 1342
Fragments arranged by size:
1342 1124 871 486 479 398 245 143
110 93 51 40 11
BmgBI CAC'GTC
Cuts at: 1409 1743 1409
Size: 334 5059
BmrI ACTGGGnnnn_n'
Cuts at: 203 1600 2605 2686 3021 5165 203
Size: 1397 1005 81 335 2144 431
Fragments arranged by size:
2144 1397 1005 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 5393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 748 1085 1768 2035 2265 619
Size: 129 337 683 267 230 3747
Fragments arranged by size:
3747 683 337 267 230 129
Bpu10I CC'TnA_GC
Cuts at: 171 295 392 1077 1366 1696 171
Size: 124 97 685 289 330 3868
Fragments arranged by size:
3868 685 330 289 124 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 2569 3448 4162 4460 1498
Size: 1071 879 714 298 2431
Fragments arranged by size:
2431 1071 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 213 5097 213
Size: 4884 509
BsaAI yAC'GTr
Cuts at: 2746 2746
Size: 5393
BsaBI GATnn'nnATC
Cuts at: 6 2907 4944 6
Size: 2901 2037 455
Fragments arranged by size:
2901 2037 455
BsaHI Gr'CG_yC
Cuts at: 1283 1763 2824 3072 3774 5270 1283
Size: 480 1061 248 702 1496 1406
Fragments arranged by size:
1496 1406 1061 702 480 248
BsaWI w'CCGG_w
Cuts at: 2582 3103 3781 4192 4339 4613 2582
Size: 521 678 411 147 274 3362
Fragments arranged by size:
3362 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 141 844 1266 1996 2317 130
Size: 11 703 422 730 321 3206
Fragments arranged by size:
3206 730 703 422 321 11
BseYI C'CCAG_C
Cuts at: 362 714 891 1241 1796 1846 2332 2818 3576
Size: 352 177 350 555 50 486 486 758
Cuts at: 3576 4241 4649 362
Size: 665 408 1106
Fragments arranged by size:
1106 758 665 555 486 486 408 352
350 177 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 982 1857 2290 298
Size: 684 875 433 3401
Fragments arranged by size:
3401 875 684 433
BsiEI CG_ry'CG
Cuts at: 104 128 137 2935 2981 4211 104
Size: 24 9 2798 46 1230 1286
Fragments arranged by size:
2798 1286 1230 46 24 9
BsiHKAI G_wGCw'C
Cuts at: 734 1120 1898 2147 2407 3185 3375 4235 734
Size: 386 778 249 260 778 190 860 1892
Fragments arranged by size:
1892 860 778 778 386 260 249 190
BsmI GAATG_Cn'
Cuts at: 1172 1448 1560 2203 1172
Size: 276 112 643 4362
Fragments arranged by size:
4362 643 276 112
BsmBI CGTCTCn'nnnn_
Cuts at: 2245 2245
Size: 5393
Bsp1286I G_dGCh'C
Cuts at: 184 235 275 286 293 734 765 774 794
Size: 51 40 11 7 441 31 9 20
Cuts at: 794 1010 1078 1101 1120 1135 1596 1606 1898
Size: 216 68 23 19 15 461 10 292
Cuts at: 1898 2004 2147 2407 3018 3111 3185 3375 3437
Size: 106 143 260 611 93 74 190 62
Cuts at: 3437 4235 184
Size: 798 1342
Fragments arranged by size:
1342 798 611 461 441 292 260 216
190 143 106 93 74 68 62 51
40 31 23 20 19 15 11 10
9 7
BspHI T'CATG_A
Cuts at: 3825 3825
Size: 5393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 229 1193 1535 2185 2443 2657 2959 3340 229
Size: 964 342 650 258 214 302 381 2282
Fragments arranged by size:
2282 964 650 381 342 302 258 214
BsrBI CCG'CTC
Cuts at: 50 124 165 722 1961 3685 3739 5223 5388
Size: 74 41 557 1239 1724 54 1484 165
Cuts at: 5388 50
Size: 55
Fragments arranged by size:
1724 1484 1239 557 165 74 55 54
41
BsrDI GCAATG_nn'
Cuts at: 2345 3305 2345
Size: 960 4433
BsrFI r'CCGG_y
Cuts at: 2139 3391 3572 2139
Size: 1252 181 3960
Fragments arranged by size:
3960 1252 181
BssHII G'CGCG_C
Cuts at: 337 375 337
Size: 38 5355
BssSI C'ACGA_G
Cuts at: 328 2299 3664 4372 328
Size: 1971 1365 708 1349
Fragments arranged by size:
1971 1365 1349 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2770 2770
Size: 5393
BstBI TT'CG_AA
Cuts at: 344 3754 344
Size: 3410 1983
BstEII G'GTnAC_C
Cuts at: 591 591
Size: 5393
BstXI CCAn_nnnn'nTGG
Cuts at: 1002 1914 1002
Size: 912 4481
BstYI r'GATC_y
Cuts at: 1 308 899 1338 1704 2418 2885 2908 3243
Size: 307 591 439 366 714 467 23 335
Cuts at: 3243 3489 3795 3807 3893 4945 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 714 591 467 449 439 366 335
307 306 246 86 23 12
BtgI C'CryG_G
Cuts at: 131 275 581 1755 2150 2186 131
Size: 144 306 1174 395 36 3338
Fragments arranged by size:
3338 1174 395 306 144 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3343 3524 3687 3343
Size: 181 163 5049
Fragments arranged by size:
5049 181 163
BtsI GCAGTG_nn'
Cuts at: 749 2004 2636 749
Size: 1255 632 3506
Fragments arranged by size:
3506 1255 632
DraI TTT'AAA
Cuts at: 2392 2392
Size: 5393
DraIII CAC_nnn'GTG
Cuts at: 1947 1947
Size: 5393
DrdI GACnn_nn'nnGTC
Cuts at: 3099 4443 3099
Size: 1344 4049
EaeI y'GGCC_r
Cuts at: 125 1249 1544 2372 2978 3152 3543 3570 4915
Size: 1124 295 828 606 174 391 27 1345
Cuts at: 4915 125
Size: 603
Fragments arranged by size:
1345 1124 828 606 603 391 295 174
27
EagI C'GGCC_G
Cuts at: 125 2978 125
Size: 2853 2540
EarI CTCTTCn'nnn_
Cuts at: 650 3416 3626 650
Size: 2766 210 2417
Fragments arranged by size:
2766 2417 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 201 272 471 3601 4343 4489 5203 201
Size: 71 199 3130 742 146 714 391
Fragments arranged by size:
3130 742 714 391 199 146 71
EcoICRI GAG'CTC
Cuts at: 732 2405 732
Size: 1673 3720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 502 532 619 748 1085 1767 1768 2035
Size: 111 30 87 129 337 682 1 267
Cuts at: 2035 2265 3218 3650 4003 4665 391
Size: 230 953 432 353 662 1119
Fragments arranged by size:
1119 953 682 662 432 353 337 267
230 129 111 87 30 1
EcoNI CCTnn'n_nnAGG
Cuts at: 532 655 1531 2374 532
Size: 123 876 843 3551
Fragments arranged by size:
3551 876 843 123
EcoO109I rG'GnC_Cy
Cuts at: 181 761 762 781 1602 1773 1842 1902 2340
Size: 580 1 19 821 171 69 60 438
Cuts at: 2340 2532 181
Size: 192 3042
Fragments arranged by size:
3042 821 580 438 192 171 69 60
19 1
EcoRI G'AATT_C
Cuts at: 2397 2397
Size: 5393
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 5393
FspI TGC'GCA
Cuts at: 3174 3174
Size: 5393
HaeII r_GCGC'y
Cuts at: 876 1564 1803 2363 2827 3075 4305 5076 876
Size: 688 239 560 464 248 1230 771 1193
Fragments arranged by size:
1230 1193 771 688 560 464 248 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 676 708 3235 3265 3267 3297 676
Size: 32 2527 30 2 30 2772
Fragments arranged by size:
2772 2527 32 30 30 2
HincII GTy'rAC
Cuts at: 2432 2432
Size: 5393
KasI G'GCGC_C
Cuts at: 2823 3071 2823
Size: 248 5145
KpnI G_GTAC'C
Cuts at: 2413 2413
Size: 5393
MluI A'CGCG_T
Cuts at: 4826 4826
Size: 5393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 666 1660 2062 4152 4336 666
Size: 994 402 2090 184 1723
Fragments arranged by size:
2090 1723 994 402 184
MscI TGG'CCA
Cuts at: 2374 3154 2374
Size: 780 4613
MslI CAynn'nnrTG
Cuts at: 1912 3382 3509 1912
Size: 1470 127 3796
Fragments arranged by size:
3796 1470 127
MspA1I CmG'CkG
Cuts at: 133 362 1370 2033 2504 2818 3178 3962 4207
Size: 229 1008 663 471 314 360 784 245
Cuts at: 4207 133
Size: 1319
Fragments arranged by size:
1319 1008 784 663 471 360 314 245
229
NaeI GCC'GGC
Cuts at: 3574 3574
Size: 5393
NarI GG'CG_CC
Cuts at: 2824 3072 2824
Size: 248 5145
NcoI C'CATG_G
Cuts at: 2150 2150
Size: 5393
NgoMIV G'CCGG_C
Cuts at: 3572 3572
Size: 5393
NotI GC'GGCC_GC
Cuts at: 125 125
Size: 5393
NspI r_CATG'y
Cuts at: 690 1585 2446 2946 4549 690
Size: 895 861 500 1603 1534
Fragments arranged by size:
1603 1534 895 861 500
PasI CC'CwG_GG
Cuts at: 767 767
Size: 5393
PciI A'CATG_T
Cuts at: 4545 4545
Size: 5393
PflMI CCAn_nnn'nTGG
Cuts at: 1143 1746 1803 1947 1143
Size: 603 57 144 4589
Fragments arranged by size:
4589 603 144 57
PfoI T'CCnGG_A
Cuts at: 1745 2117 1745
Size: 372 5021
PmeI GTTT'AAAC
Cuts at: 2392 2392
Size: 5393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 5361
PpuMI rG'GwC_Cy
Cuts at: 1842 1902 1842
Size: 60 5333
PspOMI G'GGCC_C
Cuts at: 180 231 271 282 761 1602 180
Size: 51 40 11 479 841 3971
Fragments arranged by size:
3971 841 479 51 40 11
PstI C_TGCA'G
Cuts at: 789 914 1532 2440 3125 789
Size: 125 618 908 685 3057
Fragments arranged by size:
3057 908 685 618 125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5393
PvuII CAG'CTG
Cuts at: 1370 2818 3178 1370
Size: 1448 360 3585
Fragments arranged by size:
3585 1448 360
RsrII CG'GwC_CG
Cuts at: 3588 3588
Size: 5393
SacI G_AGCT'C
Cuts at: 734 2407 734
Size: 1673 3720
SacII CC_GC'GG
Cuts at: 134 134
Size: 5393
SalI G'TCGA_C
Cuts at: 2430 2430
Size: 5393
SanDI GG'GwC_CC
Cuts at: 1842 1902 1842
Size: 60 5333
SapI GCTCTTCn'nnn_
Cuts at: 3416 3626 3416
Size: 210 5183
SbfI CC_TGCA'GG
Cuts at: 789 2440 789
Size: 1651 3742
ScaI AGT'ACT
Cuts at: 4910 4910
Size: 5393
SexAI A'CCwGG_T
Cuts at: 446 446
Size: 5393
SfcI C'TryA_G
Cuts at: 33 785 910 1528 1647 2436 3121 4089 4280
Size: 752 125 618 119 789 685 968 191
Cuts at: 4280 33
Size: 1146
Fragments arranged by size:
1146 968 789 752 685 618 191 125
119
SfoI GGC'GCC
Cuts at: 2825 3073 2825
Size: 248 5145
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
SmaI CCC'GGG
Cuts at: 225 270 2023 2415 225
Size: 45 1753 392 3203
Fragments arranged by size:
3203 1753 392 45
SmlI C'TyrA_G
Cuts at: 1513 2548 3463 4177 4439 1513
Size: 1035 915 714 262 2467
Fragments arranged by size:
2467 1035 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5393
SphI G_CATG'C
Cuts at: 2446 2946 2446
Size: 500 4893
StyI C'CwwG_G
Cuts at: 995 1167 2150 2375 2527 2881 995
Size: 172 983 225 152 354 3507
Fragments arranged by size:
3507 983 354 225 172 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 579 2976 3109 3568 3777 579
Size: 2397 133 459 209 2195
Fragments arranged by size:
2397 2195 459 209 133
TatI w'GTAC_w
Cuts at: 4908 4908
Size: 5393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1753 1785 4910 4942 1753
Size: 32 3125 32 2204
Fragments arranged by size:
3125 2204 32 32
Tth111I GACn'n_nGTC
Cuts at: 1972 2170 3190 1972
Size: 198 1020 4175
Fragments arranged by size:
4175 1020 198
XbaI T'CTAG_A
Cuts at: 2424 2424
Size: 5393
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 2888 579
Size: 2309 3084
XmaI C'CCGG_G
Cuts at: 223 268 2021 2413 223
Size: 45 1753 392 3203
Fragments arranged by size:
3203 1753 392 45
ZraI GAC'GTC
Cuts at: 1284 1284
Size: 5393
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI AvrII BaeI BcgI BfrBI
BmtI BsaXI BsiWI BspEI BsrGI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI