Restriction map for
pF1KB3523
(Circular) (Six-base) MAPSORT of: pF1KB3523.seq Check: 1486 from: 1 to: 5687
pF1KB3523 5687 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 621 621
Size: 5687
AccI GT'mk_AC
Cuts at: 314 2138 2725 314
Size: 1824 587 3276
Fragments arranged by size:
3276 1824 587
Acc65I G'GTAC_C
Cuts at: 2703 2703
Size: 5687
AclI AA'CG_TT
Cuts at: 5527 5527
Size: 5687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 979 1125 1677 2370 2432 3512 3944 4297
Size: 346 146 552 693 62 1080 432 353
Cuts at: 4297 4959 633
Size: 662 1361
Fragments arranged by size:
1361 1080 693 662 552 432 353 346
146 62
AfeI AGC'GCT
Cuts at: 2339 2339
Size: 5687
AflIII A'CryG_T
Cuts at: 251 878 3039 4839 5120 251
Size: 627 2161 1800 281 818
Fragments arranged by size:
2161 1800 818 627 281
AhdI GACnn_n'nnGTC
Cuts at: 2485 2485
Size: 5687
AleI CACnn'nnGTG
Cuts at: 1234 1234
Size: 5687
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 970 1427 1771 1942 970
Size: 457 344 171 4715
Fragments arranged by size:
4715 457 344 171
AlwNI CAG_nnn'CTG
Cuts at: 370 1774 2992 4430 370
Size: 1404 1218 1438 1627
Fragments arranged by size:
1627 1438 1404 1218
ApaI G_GGCC'C
Cuts at: 1683 1722 1683
Size: 39 5648
ApaLI G'TGCA_C
Cuts at: 4525 4525
Size: 5687
ApoI r'AATT_y
Cuts at: 1387 2691 5040 5296 1387
Size: 1304 2349 256 1778
Fragments arranged by size:
2349 1778 1304 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5687
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
AvaI C'yCGr_G
Cuts at: 503 2318 2707 503
Size: 1815 389 3483
Fragments arranged by size:
3483 1815 389
BamHI G'GATC_C
Cuts at: 2712 2712
Size: 5687
BanI G'GyrC_C
Cuts at: 338 853 1311 1686 1854 2539 2703 3117 3365
Size: 515 458 375 168 685 164 414 248
Cuts at: 3365 3400 338
Size: 35 2625
Fragments arranged by size:
2625 685 515 458 414 375 248 168
164 35
BanII G_rGCy'C
Cuts at: 1468 1683 1722 1898 1988 2251 2701 3731 1468
Size: 215 39 176 90 263 450 1030 3424
Fragments arranged by size:
3424 1030 450 263 215 176 90 39
BbeI G_GCGC'C
Cuts at: 342 3121 3369 342
Size: 2779 248 2660
Fragments arranged by size:
2779 2660 248
BbsI GAAGACnn'nnnn_
Cuts at: 976 2024 2030 976
Size: 1048 6 4633
Fragments arranged by size:
4633 1048 6
BbvCI CC'TCA_GC
Cuts at: 2162 2162
Size: 5687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2389 2423 2389
Size: 34 5653
BciVI GTATCCnnnnn_n'
Cuts at: 3585 4641 5687 3585
Size: 1056 1046 3585
Fragments arranged by size:
3585 1056 1046
BclI T'GATC_A
Cuts at: 3207 3207
Size: 5687
BglI GCCn_nnn'nGGC
Cuts at: 399 1717 1759 399
Size: 1318 42 4327
Fragments arranged by size:
4327 1318 42
BglII A'GATC_T
Cuts at: 1187 3202 5239 1187
Size: 2015 2037 1635
Fragments arranged by size:
2037 2015 1635
BlpI GC'TnA_GC
Cuts at: 926 2442 2799 926
Size: 1516 357 3814
Fragments arranged by size:
3814 1516 357
Bme1580I G_kGCm'C
Cuts at: 856 1583 1683 1691 1722 1859 1921 2542 3312
Size: 727 100 8 31 137 62 621 770
Cuts at: 3312 3405 4529 856
Size: 93 1124 2014
Fragments arranged by size:
2014 1124 770 727 621 137 100 93
62 31 8
BmgBI CAC'GTC
Cuts at: 879 879
Size: 5687
BmrI ACTGGGnnnn_n'
Cuts at: 523 1860 1902 2187 2899 2980 3315 5459 523
Size: 1337 42 285 712 81 335 2144 751
Fragments arranged by size:
2144 1337 751 712 335 285 81 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2457 4054 2457
Size: 1597 4090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 392 514 1201 1636 1726 1764 191
Size: 201 122 687 435 90 38 4114
Fragments arranged by size:
4114 687 435 201 122 90 38
Bpu10I CC'TnA_GC
Cuts at: 433 1724 2162 433
Size: 1291 438 3958
Fragments arranged by size:
3958 1291 438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1042 2863 3742 4456 4754 640
Size: 402 1821 879 714 298 1573
Fragments arranged by size:
1821 1573 879 714 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 167 368 5391 167
Size: 201 5023 463
Fragments arranged by size:
5023 463 201
BsaAI yAC'GTr
Cuts at: 3040 3040
Size: 5687
BsaBI GATnn'nnATC
Cuts at: 6 3201 5238 6
Size: 3195 2037 455
Fragments arranged by size:
3195 2037 455
BsaHI Gr'CG_yC
Cuts at: 339 2120 3118 3366 4068 5564 339
Size: 1781 998 248 702 1496 462
Fragments arranged by size:
1781 1496 998 702 462 248
BsaWI w'CCGG_w
Cuts at: 1091 1287 1977 2876 3397 4075 4486 4633 4907
Size: 196 690 899 521 678 411 147 274
Cuts at: 4907 1091
Size: 1871
Fragments arranged by size:
1871 899 690 678 521 411 274 196
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 164 194 571 601 863 893 2011 2041 164
Size: 30 377 30 262 30 1118 30 3810
Fragments arranged by size:
3810 1118 377 262 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 364 454 1621 1639 1663 1906 1954 1957
Size: 225 90 1167 18 24 243 48 3
Cuts at: 1957 2040 139
Size: 83 3786
Fragments arranged by size:
3786 1167 243 225 90 83 48 24
18 3
BseYI C'CCAG_C
Cuts at: 849 916 1245 1547 3112 3870 4535 4943 849
Size: 67 329 302 1565 758 665 408 1593
Fragments arranged by size:
1593 1565 758 665 408 329 302 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 625 575
Size: 50 5637
BsiEI CG_ry'CG
Cuts at: 104 578 3229 3275 4505 104
Size: 474 2651 46 1230 1286
Fragments arranged by size:
2651 1286 1230 474 46
BsiHKAI G_wGCw'C
Cuts at: 706 815 932 1139 1468 1750 1898 2701 3479
Size: 109 117 207 329 282 148 803 778
Cuts at: 3479 3669 4529 706
Size: 190 860 1864
Fragments arranged by size:
1864 860 803 778 329 282 207 190
148 117 109
BsmI GAATG_Cn'
Cuts at: 1203 1203
Size: 5687
Bsp1286I G_dGCh'C
Cuts at: 706 815 856 932 1139 1468 1583 1683 1691
Size: 109 41 76 207 329 115 100 8
Cuts at: 1691 1722 1750 1859 1898 1921 1988 2251 2542
Size: 31 28 109 39 23 67 263 291
Cuts at: 2542 2701 3312 3405 3479 3669 3731 4529 706
Size: 159 611 93 74 190 62 798 1864
Fragments arranged by size:
1864 798 611 329 291 263 207 190
159 115 109 109 100 93 76 74
67 62 41 39 31 28 23 8
BspEI T'CCGG_A
Cuts at: 1091 1977 1091
Size: 886 4801
BspHI T'CATG_A
Cuts at: 4119 4119
Size: 5687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 621 1719 2737 2951 3253 3634 621
Size: 1098 1018 214 302 381 2674
Fragments arranged by size:
2674 1098 1018 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1308 2441 3979 4033 5517 5682 50
Size: 1258 1133 1538 54 1484 165 55
Fragments arranged by size:
1538 1484 1258 1133 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1340 1955 3599 1340
Size: 615 1644 3428
Fragments arranged by size:
3428 1644 615
BsrFI r'CCGG_y
Cuts at: 301 1400 3685 3866 301
Size: 1099 2285 181 2122
Fragments arranged by size:
2285 2122 1099 181
BssSI C'ACGA_G
Cuts at: 3958 4666 3958
Size: 708 4979
BstAPI GCAn_nnn'nTGC
Cuts at: 603 3064 603
Size: 2461 3226
BstBI TT'CG_AA
Cuts at: 4048 4048
Size: 5687
BstEII G'GTnAC_C
Cuts at: 546 546
Size: 5687
BstXI CCAn_nnnn'nTGG
Cuts at: 402 558 1018 1866 2646 402
Size: 156 460 848 780 3443
Fragments arranged by size:
3443 848 780 460 156
BstYI r'GATC_y
Cuts at: 1 1187 2381 2712 3179 3202 3537 3783 4089
Size: 1186 1194 331 467 23 335 246 306
Cuts at: 4089 4101 4187 5239 1
Size: 12 86 1052 449
Fragments arranged by size:
1194 1186 1052 467 449 335 331 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 164 2279 164
Size: 2115 3572
BtgI C'CryG_G
Cuts at: 107 1251 1583 2306 2420 107
Size: 1144 332 723 114 3374
Fragments arranged by size:
3374 1144 723 332 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1313 2045 3637 3818 3981 1313
Size: 732 1592 181 163 3019
Fragments arranged by size:
3019 1592 732 181 163
BtsI GCAGTG_nn'
Cuts at: 563 792 1660 2930 563
Size: 229 868 1270 3320
Fragments arranged by size:
3320 1270 868 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1773 1808 1773
Size: 35 5652
DraI TTT'AAA
Cuts at: 2686 2686
Size: 5687
DraIII CAC_nnn'GTG
Cuts at: 807 807
Size: 5687
DrdI GACnn_nn'nnGTC
Cuts at: 1813 3393 4737 1813
Size: 1580 1344 2763
Fragments arranged by size:
2763 1580 1344
EaeI y'GGCC_r
Cuts at: 303 575 1238 1523 2675 3272 3446 3837 3864
Size: 272 663 285 1152 597 174 391 27
Cuts at: 3864 5209 303
Size: 1345 781
Fragments arranged by size:
1345 1152 781 663 597 391 285 272
174 27
EagI C'GGCC_G
Cuts at: 575 3272 575
Size: 2697 2990
EarI CTCTTCn'nnn_
Cuts at: 1144 1640 3710 3920 1144
Size: 496 2070 210 2911
Fragments arranged by size:
2911 2070 496 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 345 437 3895 4637 4783 5497 127
Size: 218 92 3458 742 146 714 317
Fragments arranged by size:
3458 742 714 317 218 146 92
EcoICRI GAG'CTC
Cuts at: 1466 1896 2699 1466
Size: 430 803 4454
Fragments arranged by size:
4454 803 430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 392 514 633 979 1125 1201 1636 1677
Size: 201 122 119 346 146 76 435 41
Cuts at: 1677 1726 1764 2370 2432 3512 3944 4297 4959
Size: 49 38 606 62 1080 432 353 662
Cuts at: 4959 191
Size: 919
Fragments arranged by size:
1080 919 662 606 435 432 353 346
201 146 122 119 76 62 49 41
38
EcoNI CCTnn'n_nnAGG
Cuts at: 362 436 362
Size: 74 5613
EcoO109I rG'GnC_Cy
Cuts at: 159 231 354 564 1362 1718 1719 2479 2643
Size: 72 123 210 798 356 1 760 164
Cuts at: 2643 2826 159
Size: 183 3020
Fragments arranged by size:
3020 798 760 356 210 183 164 123
72 1
EcoRI G'AATT_C
Cuts at: 1387 2691 1387
Size: 1304 4383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 663 1627 1854 2014 3706 663
Size: 964 227 160 1692 2644
Fragments arranged by size:
2644 1692 964 227 160
FspI TGC'GCA
Cuts at: 3468 3468
Size: 5687
HaeII r_GCGC'y
Cuts at: 133 342 2341 3121 3369 4599 5370 133
Size: 209 1999 780 248 1230 771 450
Fragments arranged by size:
1999 1230 780 771 450 248 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 571 603 868 900 919 951 2011
Size: 32 375 32 265 32 19 32 1060
Cuts at: 2011 2043 2373 2405 2443 2471 2475 2503 2582
Size: 32 330 32 38 28 4 28 79
Cuts at: 2582 2614 3529 3559 3561 3591 164
Size: 32 915 30 2 30 2260
Fragments arranged by size:
2260 1060 915 375 330 265 79 38
32 32 32 32 32 32 32 30
30 28 28 19 4 2
HincII GTy'rAC
Cuts at: 1557 2726 1557
Size: 1169 4518
KasI G'GCGC_C
Cuts at: 338 3117 3365 338
Size: 2779 248 2660
Fragments arranged by size:
2779 2660 248
KpnI G_GTAC'C
Cuts at: 2707 2707
Size: 5687
MluI A'CGCG_T
Cuts at: 5120 5120
Size: 5687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 644 974 2080 4446 4630 644
Size: 330 1106 2366 184 1701
Fragments arranged by size:
2366 1701 1106 330 184
MscI TGG'CCA
Cuts at: 1240 1525 2677 3448 1240
Size: 285 1152 771 3479
Fragments arranged by size:
3479 1152 771 285
MslI CAynn'nnrTG
Cuts at: 244 952 1234 1882 2195 3676 3803 244
Size: 708 282 648 313 1481 127 2128
Fragments arranged by size:
2128 1481 708 648 313 282 127
MspA1I CmG'CkG
Cuts at: 1014 1109 1572 2094 2156 2446 2798 3112 3472
Size: 95 463 522 62 290 352 314 360
Cuts at: 3472 4256 4501 1014
Size: 784 245 2200
Fragments arranged by size:
2200 784 522 463 360 352 314 290
245 95 62
NaeI GCC'GGC
Cuts at: 3868 3868
Size: 5687
NarI GG'CG_CC
Cuts at: 339 3118 3366 339
Size: 2779 248 2660
Fragments arranged by size:
2779 2660 248
NcoI C'CATG_G
Cuts at: 107 1251 1583 2306 2420 107
Size: 1144 332 723 114 3374
Fragments arranged by size:
3374 1144 723 332 114
NdeI CA'TA_TG
Cuts at: 888 888
Size: 5687
NgoMIV G'CCGG_C
Cuts at: 3866 3866
Size: 5687
NspI r_CATG'y
Cuts at: 646 957 2740 3240 4843 646
Size: 311 1783 500 1603 1490
Fragments arranged by size:
1783 1603 1490 500 311
PciI A'CATG_T
Cuts at: 4839 4839
Size: 5687
PflMI CCAn_nnn'nTGG
Cuts at: 807 2097 2177 2673 807
Size: 1290 80 496 3821
Fragments arranged by size:
3821 1290 496 80
PfoI T'CCnGG_A
Cuts at: 908 1037 2489 2499 908
Size: 129 1452 10 4096
Fragments arranged by size:
4096 1452 129 10
PmeI GTTT'AAAC
Cuts at: 2686 2686
Size: 5687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 4133
Size: 32 5655
PpuMI rG'GwC_Cy
Cuts at: 159 354 2479 2643 159
Size: 195 2125 164 3203
Fragments arranged by size:
3203 2125 195 164
PspOMI G'GGCC_C
Cuts at: 1679 1718 1679
Size: 39 5648
PstI C_TGCA'G
Cuts at: 1760 1931 2734 3419 1760
Size: 171 803 685 4028
Fragments arranged by size:
4028 803 685 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5687
PvuII CAG'CTG
Cuts at: 1109 1572 2094 2446 3112 3472 1109
Size: 463 522 352 666 360 3324
Fragments arranged by size:
3324 666 522 463 360 352
RsrII CG'GwC_CG
Cuts at: 3882 3882
Size: 5687
SacI G_AGCT'C
Cuts at: 1468 1898 2701 1468
Size: 430 803 4454
Fragments arranged by size:
4454 803 430
SalI G'TCGA_C
Cuts at: 2724 2724
Size: 5687
SanDI GG'GwC_CC
Cuts at: 159 159
Size: 5687
SapI GCTCTTCn'nnn_
Cuts at: 1144 3710 3920 1144
Size: 2566 210 2911
Fragments arranged by size:
2911 2566 210
SbfI CC_TGCA'GG
Cuts at: 1760 2734 1760
Size: 974 4713
ScaI AGT'ACT
Cuts at: 5204 5204
Size: 5687
SexAI A'CCwGG_T
Cuts at: 1811 1811
Size: 5687
SfcI C'TryA_G
Cuts at: 33 362 1756 1927 2730 3415 4383 4574 33
Size: 329 1394 171 803 685 968 191 1146
Fragments arranged by size:
1394 1146 968 803 685 329 191 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 1717 1759 1717
Size: 42 5645
SfoI GGC'GCC
Cuts at: 340 3119 3367 340
Size: 2779 248 2660
Fragments arranged by size:
2779 2660 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
SmaI CCC'GGG
Cuts at: 505 2320 2709 505
Size: 1815 389 3483
Fragments arranged by size:
3483 1815 389
SmlI C'TyrA_G
Cuts at: 655 1057 2842 3757 4471 4733 655
Size: 402 1785 915 714 262 1609
Fragments arranged by size:
1785 1609 915 714 402 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5687
SphI G_CATG'C
Cuts at: 646 2740 3240 646
Size: 2094 500 3093
Fragments arranged by size:
3093 2094 500
StuI AGG'CCT
Cuts at: 1711 2329 1711
Size: 618 5069
StyI C'CwwG_G
Cuts at: 107 1251 1583 1998 2306 2420 2639 2821 3175
Size: 1144 332 415 308 114 219 182 354
Cuts at: 3175 107
Size: 2619
Fragments arranged by size:
2619 1144 415 354 332 308 219 182
114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1246 1561 1842 2583 3270 3403 3862 4071 1246
Size: 315 281 741 687 133 459 209 2862
Fragments arranged by size:
2862 741 687 459 315 281 209 133
TatI w'GTAC_w
Cuts at: 881 1541 5202 881
Size: 660 3661 1366
Fragments arranged by size:
3661 1366 660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 863 895 5204 5236 863
Size: 32 4309 32 1314
Fragments arranged by size:
4309 1314 32 32
Tth111I GACn'n_nGTC
Cuts at: 178 532 904 3484 178
Size: 354 372 2580 2381
Fragments arranged by size:
2580 2381 372 354
XbaI T'CTAG_A
Cuts at: 2718 2718
Size: 5687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2304 3182 2304
Size: 878 4809
XmaI C'CCGG_G
Cuts at: 503 2318 2707 503
Size: 1815 389 3483
Fragments arranged by size:
3483 1815 389
XmnI GAAnn'nnTTC
Cuts at: 1189 1673 1189
Size: 484 5203
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SgrAI SnaBI SrfI SspI SwaI XhoI ZraI