Restriction map for
pF1KB3524
(Circular) (Six-base) MAPSORT of: pF1KB3524.seq Check: 5901 from: 1 to: 4493
pF1KB3524 4493 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 564 564
Size: 4493
AccI GT'mk_AC
Cuts at: 417 1531 417
Size: 1114 3379
Acc65I G'GTAC_C
Cuts at: 1509 1509
Size: 4493
AclI AA'CG_TT
Cuts at: 857 4333 857
Size: 3476 1017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1051 1214 2318 2750 3497 3765 925
Size: 126 163 1104 432 747 268 1653
Fragments arranged by size:
1653 1104 747 432 268 163 126
AflIII A'CryG_T
Cuts at: 1845 2949 3926 1845
Size: 1104 977 2412
Fragments arranged by size:
2412 1104 977
AgeI A'CCGG_T
Cuts at: 2881 2881
Size: 4493
AlwNI CAG_nnn'CTG
Cuts at: 622 943 1798 3365 622
Size: 321 855 1567 1750
Fragments arranged by size:
1750 1567 855 321
ApaLI G'TGCA_C
Cuts at: 3263 3263
Size: 4493
ApoI r'AATT_y
Cuts at: 490 502 824 970 1497 3846 4102 490
Size: 12 322 146 527 2349 256 881
Fragments arranged by size:
2349 881 527 322 256 146 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4493
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
AvaI C'yCGr_G
Cuts at: 187 1513 187
Size: 1326 3167
BamHI G'GATC_C
Cuts at: 813 1518 813
Size: 705 3788
BanI G'GyrC_C
Cuts at: 1509 1923 2171 2206 1509
Size: 414 248 35 3796
Fragments arranged by size:
3796 414 248 35
BanII G_rGCy'C
Cuts at: 188 953 1507 2537 188
Size: 765 554 1030 2144
Fragments arranged by size:
2144 1030 765 554
BbeI G_GCGC'C
Cuts at: 1927 2175 1927
Size: 248 4245
BbsI GAAGACnn'nnnn_
Cuts at: 1481 1481
Size: 4493
BciVI GTATCCnnnnn_n'
Cuts at: 2391 3152 4493 2391
Size: 761 1341 2391
Fragments arranged by size:
2391 1341 761
BclI T'GATC_A
Cuts at: 2013 2013
Size: 4493
BglII A'GATC_T
Cuts at: 2008 4045 2008
Size: 2037 2456
BlpI GC'TnA_GC
Cuts at: 1605 1605
Size: 4493
Bme1580I G_kGCm'C
Cuts at: 2118 2211 3267 2118
Size: 93 1056 3344
Fragments arranged by size:
3344 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1705 1786 2121 4265 1705
Size: 81 335 2144 1933
Fragments arranged by size:
2144 1933 335 81
BmtI G_CTAG'C
Cuts at: 1045 1045
Size: 4493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 4493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 809 220
Size: 589 3904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1480 1669 2548 3040 3338 1480
Size: 189 879 492 298 2635
Fragments arranged by size:
2635 879 492 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 4197 4197
Size: 4493
BsaAI yAC'GTr
Cuts at: 1297 1846 1297
Size: 549 3944
BsaBI GATnn'nnATC
Cuts at: 6 1058 2007 4044 6
Size: 1052 949 2037 455
Fragments arranged by size:
2037 1052 949 455
BsaHI Gr'CG_yC
Cuts at: 561 1924 2172 2874 4370 561
Size: 1363 248 702 1496 684
Fragments arranged by size:
1496 1363 702 684 248
BsaWI w'CCGG_w
Cuts at: 1682 2203 2881 3155 3302 3713 1682
Size: 521 678 274 147 411 2462
Fragments arranged by size:
2462 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 855 885 855
Size: 30 4463
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 688 763 766 769 772 775 778 781 823
Size: 75 3 3 3 3 3 3 42
Cuts at: 823 1223 688
Size: 400 3958
Fragments arranged by size:
3958 400 75 42 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 223 265 307 1918 2676 3253 3749 223
Size: 42 42 1611 758 577 496 967
Fragments arranged by size:
1611 967 758 577 496 42 42
BsiEI CG_ry'CG
Cuts at: 104 2035 2081 3289 104
Size: 1931 46 1208 1308
Fragments arranged by size:
1931 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1507 2285 2475 3267 1507
Size: 778 190 792 2733
Fragments arranged by size:
2733 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 227 227
Size: 4493
Bsp1286I G_dGCh'C
Cuts at: 188 953 1507 2118 2211 2285 2475 2537 3267
Size: 765 554 611 93 74 190 62 730
Cuts at: 3267 188
Size: 1414
Fragments arranged by size:
1414 765 730 611 554 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3713 3713
Size: 4493
BspHI T'CATG_A
Cuts at: 507 651 3669 507
Size: 144 3018 1331
Fragments arranged by size:
3018 1331 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1543 1757 2059 2440 1543
Size: 214 302 381 3596
Fragments arranged by size:
3596 381 302 214
BsrBI CCG'CTC
Cuts at: 50 472 2785 2839 4323 4488 50
Size: 422 2313 54 1484 165 55
Fragments arranged by size:
2313 1484 422 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2405 2405
Size: 4493
BsrFI r'CCGG_y
Cuts at: 2491 2672 2881 2491
Size: 181 209 4103
Fragments arranged by size:
4103 209 181
BssSI C'ACGA_G
Cuts at: 2764 3122 2764
Size: 358 4135
BstAPI GCAn_nnn'nTGC
Cuts at: 1870 1870
Size: 4493
BstBI TT'CG_AA
Cuts at: 2854 2854
Size: 4493
BstYI r'GATC_y
Cuts at: 1 813 939 1166 1518 1985 2008 2343 2589
Size: 812 126 227 352 467 23 335 246
Cuts at: 2589 3601 3687 3699 4045 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 812 467 449 352 346 335 246
227 126 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 496 818 496
Size: 322 4171
BtgI C'CryG_G
Cuts at: 107 107
Size: 4493
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2443 2624 2787 2443
Size: 181 163 4149
Fragments arranged by size:
4149 181 163
BtsI GCAGTG_nn'
Cuts at: 1736 1736
Size: 4493
DraI TTT'AAA
Cuts at: 851 1492 851
Size: 641 3852
DrdI GACnn_nn'nnGTC
Cuts at: 2199 3057 2199
Size: 858 3635
EaeI y'GGCC_r
Cuts at: 110 473 2078 2252 2643 2670 4015 110
Size: 363 1605 174 391 27 1345 588
Fragments arranged by size:
1605 1345 588 391 363 174 27
EagI C'GGCC_G
Cuts at: 2078 2078
Size: 4493
EarI CTCTTCn'nnn_
Cuts at: 149 590 650 740 788 800 2516 2726 149
Size: 441 60 90 48 12 1716 210 1916
Fragments arranged by size:
1916 1716 441 210 90 60 48 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2701 3011 3157 4303 2701
Size: 310 146 1146 2891
Fragments arranged by size:
2891 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1505 1505
Size: 4493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 809 925 1051 1214 2318 2750 3497 3765
Size: 589 116 126 163 1104 432 747 268
Cuts at: 3765 220
Size: 948
Fragments arranged by size:
1104 948 747 589 432 268 163 126
116
EcoO109I rG'GnC_Cy
Cuts at: 1632 1632
Size: 4493
EcoRI G'AATT_C
Cuts at: 824 1497 824
Size: 673 3820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 949 2512 949
Size: 1563 2930
FspI TGC'GCA
Cuts at: 2274 2274
Size: 4493
HaeII r_GCGC'y
Cuts at: 1927 2175 3197 4176 1927
Size: 248 1022 979 2244
Fragments arranged by size:
2244 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 551 583 2335 2365 2367 2397 337
Size: 32 182 32 1752 30 2 30 2433
Fragments arranged by size:
2433 1752 182 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1013 1532 1013
Size: 519 3974
HindIII A'AGCT_T
Cuts at: 457 931 1442 457
Size: 474 511 3508
Fragments arranged by size:
3508 511 474
KasI G'GCGC_C
Cuts at: 1923 2171 1923
Size: 248 4245
KpnI G_GTAC'C
Cuts at: 1513 1513
Size: 4493
MfeI C'AATT_G
Cuts at: 1427 1427
Size: 4493
MluI A'CGCG_T
Cuts at: 3926 3926
Size: 4493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3164 3348 3164
Size: 184 4309
MscI TGG'CCA
Cuts at: 2254 2254
Size: 4493
MslI CAynn'nnrTG
Cuts at: 692 2482 2609 692
Size: 1790 127 2576
Fragments arranged by size:
2576 1790 127
MspA1I CmG'CkG
Cuts at: 220 265 1604 1918 2278 3291 3536 220
Size: 45 1339 314 360 1013 245 1177
Fragments arranged by size:
1339 1177 1013 360 314 245 45
NaeI GCC'GGC
Cuts at: 2674 2674
Size: 4493
NarI GG'CG_CC
Cuts at: 1924 2172 1924
Size: 248 4245
NcoI C'CATG_G
Cuts at: 107 107
Size: 4493
NgoMIV G'CCGG_C
Cuts at: 2672 2672
Size: 4493
NheI G'CTAG_C
Cuts at: 1041 1041
Size: 4493
NspI r_CATG'y
Cuts at: 1546 2046 2953 1546
Size: 500 907 3086
Fragments arranged by size:
3086 907 500
PciI A'CATG_T
Cuts at: 2949 2949
Size: 4493
PmeI GTTT'AAAC
Cuts at: 1492 1492
Size: 4493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4461
PstI C_TGCA'G
Cuts at: 1540 2225 1540
Size: 685 3808
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4493
PvuII CAG'CTG
Cuts at: 1918 2278 1918
Size: 360 4133
RsrII CG'GwC_CG
Cuts at: 2688 2688
Size: 4493
SacI G_AGCT'C
Cuts at: 1507 1507
Size: 4493
SalI G'TCGA_C
Cuts at: 1530 1530
Size: 4493
SapI GCTCTTCn'nnn_
Cuts at: 2516 2726 2516
Size: 210 4283
SbfI CC_TGCA'GG
Cuts at: 1540 1540
Size: 4493
ScaI AGT'ACT
Cuts at: 4010 4010
Size: 4493
SfcI C'TryA_G
Cuts at: 33 590 1065 1536 2221 3214 3405 33
Size: 557 475 471 685 993 191 1121
Fragments arranged by size:
1121 993 685 557 475 471 191
SfoI GGC'GCC
Cuts at: 1925 2173 1925
Size: 248 4245
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
SmaI CCC'GGG
Cuts at: 1515 1515
Size: 4493
SmlI C'TyrA_G
Cuts at: 1459 1648 2563 3055 3317 1459
Size: 189 915 492 262 2635
Fragments arranged by size:
2635 915 492 262 189
SnaBI TAC'GTA
Cuts at: 1297 1297
Size: 4493
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4493
SphI G_CATG'C
Cuts at: 1546 2046 1546
Size: 500 3993
SspI AAT'ATT
Cuts at: 994 994
Size: 4493
StyI C'CwwG_G
Cuts at: 107 1205 1627 1981 107
Size: 1098 422 354 2619
Fragments arranged by size:
2619 1098 422 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2076 2209 2668 2877 2076
Size: 133 459 209 3692
Fragments arranged by size:
3692 459 209 133
TatI w'GTAC_w
Cuts at: 4008 4008
Size: 4493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 234 266 318 350 4010 4042 234
Size: 32 52 32 3660 32 685
Fragments arranged by size:
3660 685 52 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2290 2290
Size: 4493
XbaI T'CTAG_A
Cuts at: 1524 1524
Size: 4493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1988 1988
Size: 4493
XmaI C'CCGG_G
Cuts at: 1513 1513
Size: 4493
XmnI GAAnn'nnTTC
Cuts at: 1199 1199
Size: 4493
ZraI GAC'GTC
Cuts at: 562 562
Size: 4493
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI
XhoI