Restriction map for
pF1KB3539
(Circular) (Six-base) MAPSORT of: pF1KB3539.seq Check: 6804 from: 1 to: 4145
pF1KB3539 4145 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1183 1183
Size: 4145
Acc65I G'GTAC_C
Cuts at: 699 1161 699
Size: 462 3683
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 965 1970 2402 3149 3417 617
Size: 348 1005 432 747 268 1345
Fragments arranged by size:
1345 1005 747 432 348 268
AflIII A'CryG_T
Cuts at: 1497 2601 3578 1497
Size: 1104 977 2064
Fragments arranged by size:
2064 1104 977
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AlwNI CAG_nnn'CTG
Cuts at: 564 1450 3017 564
Size: 886 1567 1692
Fragments arranged by size:
1692 1567 886
ApaLI G'TGCA_C
Cuts at: 2915 2915
Size: 4145
ApoI r'AATT_y
Cuts at: 142 926 1149 3498 3754 142
Size: 784 223 2349 256 533
Fragments arranged by size:
2349 784 533 256 223
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 703 1165 703
Size: 462 3683
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 214 699 1161 1575 1823 1858 214
Size: 485 462 414 248 35 2501
Fragments arranged by size:
2501 485 462 414 248 35
BanII G_rGCy'C
Cuts at: 914 1159 2189 914
Size: 245 1030 2870
Fragments arranged by size:
2870 1030 245
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BbsI GAAGACnn'nnnn_
Cuts at: 890 960 890
Size: 70 4075
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 1665 1665
Size: 4145
BglII A'GATC_T
Cuts at: 390 1660 3697 390
Size: 1270 2037 838
Fragments arranged by size:
2037 1270 838
BlpI GC'TnA_GC
Cuts at: 1257 1257
Size: 4145
Bme1580I G_kGCm'C
Cuts at: 1770 1863 2919 1770
Size: 93 1056 2996
Fragments arranged by size:
2996 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 782 876 1357 1438 1773 3917 782
Size: 94 481 81 335 2144 1010
Fragments arranged by size:
2144 1010 481 335 94 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 2512 640
Size: 1872 2273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1062 1062
Size: 4145
Bpu10I CC'TnA_GC
Cuts at: 820 820
Size: 4145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1321 2200 2692 2990 637
Size: 684 879 492 298 1792
Fragments arranged by size:
1792 879 684 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3849 3849
Size: 4145
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 1659 3696 6
Size: 1653 2037 455
Fragments arranged by size:
2037 1653 455
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 497 1334 1855 2533 2807 2954 3365 497
Size: 837 521 678 274 147 411 1277
Fragments arranged by size:
1277 837 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 748 748
Size: 4145
BseYI C'CCAG_C
Cuts at: 1570 2328 2905 3401 1570
Size: 758 577 496 2314
Fragments arranged by size:
2314 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1157
Size: 4145
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 914 1159 1937 2127 2919 914
Size: 245 778 190 792 2140
Fragments arranged by size:
2140 792 778 245 190
Bsp1286I G_dGCh'C
Cuts at: 914 1159 1770 1863 1937 2127 2189 2919 914
Size: 245 611 93 74 190 62 730 2140
Fragments arranged by size:
2140 730 611 245 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3365 3365
Size: 4145
BspHI T'CATG_A
Cuts at: 3321 3321
Size: 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 533 1195 1409 1711 2092 533
Size: 662 214 302 381 2586
Fragments arranged by size:
2586 662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2437 2491 3975 4140 50
Size: 2387 54 1484 165 55
Fragments arranged by size:
2387 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 996 2057 996
Size: 1061 3084
BsrFI r'CCGG_y
Cuts at: 440 2143 2324 2533 440
Size: 1703 181 209 2052
Fragments arranged by size:
2052 1703 209 181
BssSI C'ACGA_G
Cuts at: 1034 2416 2774 1034
Size: 1382 358 2405
Fragments arranged by size:
2405 1382 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1522 1522
Size: 4145
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstXI CCAn_nnnn'nTGG
Cuts at: 750 750
Size: 4145
BstYI r'GATC_y
Cuts at: 1 390 1170 1637 1660 1995 2241 3253 3339
Size: 389 780 467 23 335 246 1012 86
Cuts at: 3339 3351 3697 1
Size: 12 346 449
Fragments arranged by size:
1012 780 467 449 389 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 452 452
Size: 4145
BtgI C'CryG_G
Cuts at: 107 107
Size: 4145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2095 2276 2439 2095
Size: 181 163 3801
Fragments arranged by size:
3801 181 163
BtsI GCAGTG_nn'
Cuts at: 1388 1388
Size: 4145
DraI TTT'AAA
Cuts at: 1144 1144
Size: 4145
DraIII CAC_nnn'GTG
Cuts at: 690 1029 690
Size: 339 3806
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 1730 1904 2295 2322 3667 1730
Size: 174 391 27 1345 2208
Fragments arranged by size:
2208 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 398 2168 2378 398
Size: 1770 210 2165
Fragments arranged by size:
2165 1770 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 912 1157 912
Size: 245 3900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 965 1062 1970 2402 3149 3417 617
Size: 348 97 908 432 747 268 1345
Fragments arranged by size:
1345 908 747 432 348 268 97
EcoO109I rG'GnC_Cy
Cuts at: 1284 1284
Size: 4145
EcoRI G'AATT_C
Cuts at: 1149 1149
Size: 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 4145
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 1579 1827 2849 3828 1579
Size: 248 1022 979 1896
Fragments arranged by size:
1896 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1987 2017 2019 2049 1987
Size: 30 2 30 4083
Fragments arranged by size:
4083 30 30 2
HincII GTy'rAC
Cuts at: 164 1184 164
Size: 1020 3125
HindIII A'AGCT_T
Cuts at: 464 464
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 703 1165 703
Size: 462 3683
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2816 3000 2816
Size: 184 3961
MscI TGG'CCA
Cuts at: 1906 1906
Size: 4145
MslI CAynn'nnrTG
Cuts at: 1117 2134 2261 1117
Size: 1017 127 3001
Fragments arranged by size:
3001 1017 127
MspA1I CmG'CkG
Cuts at: 564 1256 1570 1930 2943 3188 564
Size: 692 314 360 1013 245 1521
Fragments arranged by size:
1521 1013 692 360 314 245
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 107 107
Size: 4145
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NspI r_CATG'y
Cuts at: 1198 1698 2605 1198
Size: 500 907 2738
Fragments arranged by size:
2738 907 500
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PflMI CCAn_nnn'nTGG
Cuts at: 690 690
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1050 1082 3284 3316 1050
Size: 32 2202 32 1879
Fragments arranged by size:
2202 1879 32 32
PsiI TTA'TAA
Cuts at: 1073 1073
Size: 4145
PstI C_TGCA'G
Cuts at: 554 1192 1877 554
Size: 638 685 2822
Fragments arranged by size:
2822 685 638
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 564 1570 1930 564
Size: 1006 360 2779
Fragments arranged by size:
2779 1006 360
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 914 1159 914
Size: 245 3900
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 2168 2378 2168
Size: 210 3935
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SexAI A'CCwGG_T
Cuts at: 695 695
Size: 4145
SfcI C'TryA_G
Cuts at: 33 550 1188 1873 2866 3057 33
Size: 517 638 685 993 191 1121
Fragments arranged by size:
1121 993 685 638 517 191
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 652 1300 2215 2707 2969 652
Size: 648 915 492 262 1828
Fragments arranged by size:
1828 915 648 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
StyI C'CwwG_G
Cuts at: 107 1279 1633 107
Size: 1172 354 2619
Fragments arranged by size:
2619 1172 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 176 202 1728 1861 2320 2529 176
Size: 26 1526 133 459 209 1792
Fragments arranged by size:
1792 1526 459 209 133 26
TatI w'GTAC_w
Cuts at: 3660 3660
Size: 4145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4113
Tth111I GACn'n_nGTC
Cuts at: 1942 1942
Size: 4145
XbaI T'CTAG_A
Cuts at: 642 1176 642
Size: 534 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 750 1640 750
Size: 890 3255
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
XmnI GAAnn'nnTTC
Cuts at: 850 850
Size: 4145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI