Restriction map for
pF1KB3544
(Circular) (Six-base) MAPSORT of: pF1KB3544.seq Check: 673 from: 1 to: 3437
pF1KB3544 3437 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 108 108
Size: 3437
AccI GT'mk_AC
Cuts at: 475 475
Size: 3437
Acc65I G'GTAC_C
Cuts at: 453 453
Size: 3437
AclI AA'CG_TT
Cuts at: 3277 3277
Size: 3437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1262 1694 2441 2709 1262
Size: 432 747 268 1990
Fragments arranged by size:
1990 747 432 268
AflIII A'CryG_T
Cuts at: 789 1893 2870 789
Size: 1104 977 1356
Fragments arranged by size:
1356 1104 977
AgeI A'CCGG_T
Cuts at: 1825 1825
Size: 3437
AlwNI CAG_nnn'CTG
Cuts at: 742 2309 742
Size: 1567 1870
ApaLI G'TGCA_C
Cuts at: 181 2207 181
Size: 2026 1411
ApoI r'AATT_y
Cuts at: 214 441 2790 3046 214
Size: 227 2349 256 605
Fragments arranged by size:
2349 605 256 227
AseI AT'TA_AT
Cuts at: 20 20
Size: 3437
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3437
AvaI C'yCGr_G
Cuts at: 227 457 227
Size: 230 3207
BamHI G'GATC_C
Cuts at: 462 462
Size: 3437
BanI G'GyrC_C
Cuts at: 358 368 453 867 1115 1150 358
Size: 10 85 414 248 35 2645
Fragments arranged by size:
2645 414 248 85 35 10
BanII G_rGCy'C
Cuts at: 451 1481 451
Size: 1030 2407
BbeI G_GCGC'C
Cuts at: 871 1119 871
Size: 248 3189
BbsI GAAGACnn'nnnn_
Cuts at: 396 396
Size: 3437
BciVI GTATCCnnnnn_n'
Cuts at: 1335 2096 3437 1335
Size: 761 1341 1335
Fragments arranged by size:
1341 1335 761
BclI T'GATC_A
Cuts at: 957 957
Size: 3437
BglII A'GATC_T
Cuts at: 952 2989 952
Size: 2037 1400
BlpI GC'TnA_GC
Cuts at: 549 549
Size: 3437
Bme1580I G_kGCm'C
Cuts at: 185 371 1062 1155 2211 185
Size: 186 691 93 1056 1411
Fragments arranged by size:
1411 1056 691 186 93
BmrI ACTGGGnnnn_n'
Cuts at: 374 649 730 1065 3209 374
Size: 275 81 335 2144 602
Fragments arranged by size:
2144 602 335 275 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 326
Size: 3437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1492 1984 2282 613
Size: 879 492 298 1768
Fragments arranged by size:
1768 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3141 3141
Size: 3437
BsaAI yAC'GTr
Cuts at: 790 790
Size: 3437
BsaBI GATnn'nnATC
Cuts at: 6 951 2988 6
Size: 945 2037 455
Fragments arranged by size:
2037 945 455
BsaHI Gr'CG_yC
Cuts at: 868 1116 1818 3314 868
Size: 248 702 1496 991
Fragments arranged by size:
1496 991 702 248
BsaWI w'CCGG_w
Cuts at: 626 1147 1825 2099 2246 2657 626
Size: 521 678 274 147 411 1406
Fragments arranged by size:
1406 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 104 134 324 354 104
Size: 30 190 30 3187
Fragments arranged by size:
3187 190 30 30
BseYI C'CCAG_C
Cuts at: 284 862 1620 2197 2693 284
Size: 578 758 577 496 1028
Fragments arranged by size:
1028 758 578 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 136
Size: 3437
BsiEI CG_ry'CG
Cuts at: 104 979 1025 2233 104
Size: 875 46 1208 1308
Fragments arranged by size:
1308 1208 875 46
BsiHKAI G_wGCw'C
Cuts at: 185 451 1229 1419 2211 185
Size: 266 778 190 792 1411
Fragments arranged by size:
1411 792 778 266 190
BsmBI CGTCTCn'nnnn_
Cuts at: 134 134
Size: 3437
Bsp1286I G_dGCh'C
Cuts at: 185 371 451 1062 1155 1229 1419 1481 2211
Size: 186 80 611 93 74 190 62 730
Cuts at: 2211 185
Size: 1411
Fragments arranged by size:
1411 730 611 190 186 93 80 74
62
BspEI T'CCGG_A
Cuts at: 2657 2657
Size: 3437
BspHI T'CATG_A
Cuts at: 2613 2613
Size: 3437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 108 181 487 701 1003 1384 108
Size: 73 306 214 302 381 2161
Fragments arranged by size:
2161 381 306 302 214 73
BsrBI CCG'CTC
Cuts at: 50 1729 1783 3267 3432 50
Size: 1679 54 1484 165 55
Fragments arranged by size:
1679 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1349 1349
Size: 3437
BsrFI r'CCGG_y
Cuts at: 1435 1616 1825 1435
Size: 181 209 3047
Fragments arranged by size:
3047 209 181
BssSI C'ACGA_G
Cuts at: 1708 2066 1708
Size: 358 3079
BstAPI GCAn_nnn'nTGC
Cuts at: 814 814
Size: 3437
BstBI TT'CG_AA
Cuts at: 1798 1798
Size: 3437
BstYI r'GATC_y
Cuts at: 1 462 929 952 1287 1533 2545 2631 2643
Size: 461 467 23 335 246 1012 86 12
Cuts at: 2643 2989 1
Size: 346 449
Fragments arranged by size:
1012 467 461 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1387 1568 1731 1387
Size: 181 163 3093
Fragments arranged by size:
3093 181 163
BtsI GCAGTG_nn'
Cuts at: 680 680
Size: 3437
DraI TTT'AAA
Cuts at: 436 436
Size: 3437
DrdI GACnn_nn'nnGTC
Cuts at: 1143 2001 1143
Size: 858 2579
EaeI y'GGCC_r
Cuts at: 165 1022 1196 1587 1614 2959 165
Size: 857 174 391 27 1345 643
Fragments arranged by size:
1345 857 643 391 174 27
EagI C'GGCC_G
Cuts at: 1022 1022
Size: 3437
EarI CTCTTCn'nnn_
Cuts at: 1460 1670 1460
Size: 210 3227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1645 1955 2101 3247 1645
Size: 310 146 1146 1835
Fragments arranged by size:
1835 1146 310 146
EcoICRI GAG'CTC
Cuts at: 449 449
Size: 3437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1262 1694 2441 2709 326
Size: 936 432 747 268 1054
Fragments arranged by size:
1054 936 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 576 576
Size: 3437
EcoRI G'AATT_C
Cuts at: 441 441
Size: 3437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1456 1456
Size: 3437
FspI TGC'GCA
Cuts at: 1218 1218
Size: 3437
HaeII r_GCGC'y
Cuts at: 871 1119 2141 3120 871
Size: 248 1022 979 1188
Fragments arranged by size:
1188 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 300 324 332 356 1279 1309 1311 1341 300
Size: 24 8 24 923 30 2 30 2396
Fragments arranged by size:
2396 923 30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 476 476
Size: 3437
KasI G'GCGC_C
Cuts at: 867 1115 867
Size: 248 3189
KpnI G_GTAC'C
Cuts at: 457 457
Size: 3437
MluI A'CGCG_T
Cuts at: 2870 2870
Size: 3437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2292 2108
Size: 184 3253
MscI TGG'CCA
Cuts at: 1198 1198
Size: 3437
MslI CAynn'nnrTG
Cuts at: 112 193 1426 1553 112
Size: 81 1233 127 1996
Fragments arranged by size:
1996 1233 127 81
MspA1I CmG'CkG
Cuts at: 548 862 1222 2235 2480 548
Size: 314 360 1013 245 1505
Fragments arranged by size:
1505 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1618 1618
Size: 3437
NarI GG'CG_CC
Cuts at: 868 1116 868
Size: 248 3189
NcoI C'CATG_G
Cuts at: 107 107
Size: 3437
NgoMIV G'CCGG_C
Cuts at: 1616 1616
Size: 3437
NspI r_CATG'y
Cuts at: 394 490 990 1897 394
Size: 96 500 907 1934
Fragments arranged by size:
1934 907 500 96
PciI A'CATG_T
Cuts at: 1893 1893
Size: 3437
PmeI GTTT'AAAC
Cuts at: 436 436
Size: 3437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 3405
PstI C_TGCA'G
Cuts at: 484 1169 484
Size: 685 2752
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3437
PvuII CAG'CTG
Cuts at: 862 1222 862
Size: 360 3077
RsrII CG'GwC_CG
Cuts at: 1632 1632
Size: 3437
SacI G_AGCT'C
Cuts at: 451 451
Size: 3437
SalI G'TCGA_C
Cuts at: 474 474
Size: 3437
SapI GCTCTTCn'nnn_
Cuts at: 1460 1670 1460
Size: 210 3227
SbfI CC_TGCA'GG
Cuts at: 484 484
Size: 3437
ScaI AGT'ACT
Cuts at: 2954 2954
Size: 3437
SfcI C'TryA_G
Cuts at: 33 480 1165 2158 2349 33
Size: 447 685 993 191 1121
Fragments arranged by size:
1121 993 685 447 191
SfoI GGC'GCC
Cuts at: 869 1117 869
Size: 248 3189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3437
SmaI CCC'GGG
Cuts at: 229 459 229
Size: 230 3207
SmlI C'TyrA_G
Cuts at: 592 1507 1999 2261 592
Size: 915 492 262 1768
Fragments arranged by size:
1768 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3437
SphI G_CATG'C
Cuts at: 490 990 490
Size: 500 2937
StyI C'CwwG_G
Cuts at: 107 571 925 107
Size: 464 354 2619
Fragments arranged by size:
2619 464 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 386 1020 1153 1612 1821 386
Size: 634 133 459 209 2002
Fragments arranged by size:
2002 634 459 209 133
TatI w'GTAC_w
Cuts at: 2952 2952
Size: 3437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 104 136 2954 2986 104
Size: 32 2818 32 555
Fragments arranged by size:
2818 555 32 32
Tth111I GACn'n_nGTC
Cuts at: 1234 1234
Size: 3437
XbaI T'CTAG_A
Cuts at: 468 468
Size: 3437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 932 932
Size: 3437
XmaI C'CCGG_G
Cuts at: 227 457 227
Size: 230 3207
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI