Restriction map for
pF1KB3557
(Circular) (Six-base) MAPSORT of: pF1KB3557.seq Check: 1804 from: 1 to: 4013
pF1KB3557 4013 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1051 1051
Size: 4013
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 548 1838 2270 3017 3285 151
Size: 397 1290 432 747 268 879
Fragments arranged by size:
1290 879 747 432 397 268
AflII C'TTAA_G
Cuts at: 978 978
Size: 4013
AflIII A'CryG_T
Cuts at: 389 1365 2469 3446 389
Size: 976 1104 977 956
Fragments arranged by size:
1104 977 976 956
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AleI CACnn'nnGTG
Cuts at: 793 793
Size: 4013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 811 811
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 1318 2885 1318
Size: 1567 2446
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 137 1017 3366 3622 137
Size: 880 2349 256 528
Fragments arranged by size:
2349 880 528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 1033 1033
Size: 4013
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4013
BanI G'GyrC_C
Cuts at: 1029 1443 1691 1726 1029
Size: 414 248 35 3316
Fragments arranged by size:
3316 414 248 35
BanII G_rGCy'C
Cuts at: 653 1027 2057 653
Size: 374 1030 2609
Fragments arranged by size:
2609 1030 374
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BbsI GAAGACnn'nnnn_
Cuts at: 121 314 121
Size: 193 3820
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 426 1533 426
Size: 1107 2906
BglII A'GATC_T
Cuts at: 726 1528 3565 726
Size: 802 2037 1174
Fragments arranged by size:
2037 1174 802
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 1638 1731 2787 1638
Size: 93 1056 2864
Fragments arranged by size:
2864 1056 93
BmgBI CAC'GTC
Cuts at: 328 328
Size: 4013
BmrI ACTGGGnnnn_n'
Cuts at: 1225 1306 1641 3785 1225
Size: 81 335 2144 1453
Fragments arranged by size:
2144 1453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 522
Size: 4013
Bpu10I CC'TnA_GC
Cuts at: 953 953
Size: 4013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 880 1189 2068 2560 2858 104
Size: 776 309 879 492 298 1259
Fragments arranged by size:
1259 879 776 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 595 3717 595
Size: 3122 891
BsaAI yAC'GTr
Cuts at: 390 940 1366 390
Size: 550 426 3037
Fragments arranged by size:
3037 550 426
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1202 1723 2401 2675 2822 3233 1202
Size: 521 678 274 147 411 1982
Fragments arranged by size:
1982 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1438 2196 2773 3269 1438
Size: 758 577 496 2182
Fragments arranged by size:
2182 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 817
Size: 4013
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1805 1995 2787 1027
Size: 778 190 792 2253
Fragments arranged by size:
2253 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 653 1027 1638 1731 1805 1995 2057 2787 653
Size: 374 611 93 74 190 62 730 1879
Fragments arranged by size:
1879 730 611 374 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1063 1277 1579 1960 1063
Size: 214 302 381 3116
Fragments arranged by size:
3116 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3843 4008 50
Size: 2255 54 1484 165 55
Fragments arranged by size:
2255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1925 1925
Size: 4013
BsrFI r'CCGG_y
Cuts at: 2011 2192 2401 2011
Size: 181 209 3623
Fragments arranged by size:
3623 209 181
BsrGI T'GTAC_A
Cuts at: 849 849
Size: 4013
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstYI r'GATC_y
Cuts at: 1 726 1038 1505 1528 1863 2109 3121 3207
Size: 725 312 467 23 335 246 1012 86
Cuts at: 3207 3219 3565 1
Size: 12 346 449
Fragments arranged by size:
1012 725 467 449 346 335 312 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 446 1256 446
Size: 810 3203
ClaI AT'CG_AT
Cuts at: 296 296
Size: 4013
DraI TTT'AAA
Cuts at: 626 1012 626
Size: 386 3627
DraIII CAC_nnn'GTG
Cuts at: 943 943
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 1598 1772 2163 2190 3535 1598
Size: 174 391 27 1345 2076
Fragments arranged by size:
2076 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 522 548 1838 2270 3017 3285 151
Size: 371 26 1290 432 747 268 879
Fragments arranged by size:
1290 879 747 432 371 268 26
EcoO109I rG'GnC_Cy
Cuts at: 1152 1152
Size: 4013
EcoRI G'AATT_C
Cuts at: 137 1017 137
Size: 880 3133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 1447 1695 2717 3696 1447
Size: 248 1022 979 1764
Fragments arranged by size:
1764 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1855 1885 1887 1917 1855
Size: 30 2 30 3951
Fragments arranged by size:
3951 30 30 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
HindIII A'AGCT_T
Cuts at: 445 445
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 348 2684 2868 348
Size: 2336 184 1493
Fragments arranged by size:
2336 1493 184
MscI TGG'CCA
Cuts at: 1774 1774
Size: 4013
MslI CAynn'nnrTG
Cuts at: 434 793 2002 2129 434
Size: 359 1209 127 2318
Fragments arranged by size:
2318 1209 359 127
MspA1I CmG'CkG
Cuts at: 1124 1438 1798 2811 3056 1124
Size: 314 360 1013 245 2081
Fragments arranged by size:
2081 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4013
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NcoI C'CATG_G
Cuts at: 107 107
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4013
NspI r_CATG'y
Cuts at: 1066 1566 2473 1066
Size: 500 907 2606
Fragments arranged by size:
2606 907 500
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PflMI CCAn_nnn'nTGG
Cuts at: 465 465
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PmlI CAC'GTG
Cuts at: 940 940
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 727 759 3152 3184 727
Size: 32 2393 32 1556
Fragments arranged by size:
2393 1556 32 32
PstI C_TGCA'G
Cuts at: 557 1060 1745 557
Size: 503 685 2825
Fragments arranged by size:
2825 685 503
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 1438 1798 1438
Size: 360 3653
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SexAI A'CCwGG_T
Cuts at: 174 174
Size: 4013
SfcI C'TryA_G
Cuts at: 33 244 553 1056 1741 2734 2925 33
Size: 211 309 503 685 993 191 1121
Fragments arranged by size:
1121 993 685 503 309 211 191
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4013
SmlI C'TyrA_G
Cuts at: 119 859 978 1168 2083 2575 2837 119
Size: 740 119 190 915 492 262 1295
Fragments arranged by size:
1295 915 740 492 262 190 119
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StuI AGG'CCT
Cuts at: 488 780 488
Size: 292 3721
StyI C'CwwG_G
Cuts at: 107 399 1147 1501 107
Size: 292 748 354 2619
Fragments arranged by size:
2619 748 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 446 805 1596 1729 2188 2397 446
Size: 359 791 133 459 209 2062
Fragments arranged by size:
2062 791 459 359 209 133
TatI w'GTAC_w
Cuts at: 849 3528 849
Size: 2679 1334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 3981
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 144 1044 144
Size: 900 3113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 352 1508 352
Size: 1156 2857
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4013
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI BseRI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI