Restriction map for
pF1KB3558
(Circular) (Six-base) MAPSORT of: pF1KB3558.seq Check: 310 from: 1 to: 4112
pF1KB3558 4112 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 181 181
Size: 4112
AccI GT'mk_AC
Cuts at: 181 1150 181
Size: 969 3143
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 930 1937 2369 3116 3384 930
Size: 1007 432 747 268 1658
Fragments arranged by size:
1658 1007 747 432 268
AfeI AGC'GCT
Cuts at: 432 598 432
Size: 166 3946
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AhdI GACnn_n'nnGTC
Cuts at: 831 831
Size: 4112
AleI CACnn'nnGTG
Cuts at: 727 727
Size: 4112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 810 1025 810
Size: 215 3897
AlwNI CAG_nnn'CTG
Cuts at: 738 868 993 1417 2984 738
Size: 130 125 424 1567 1866
Fragments arranged by size:
1866 1567 424 130 125
ApaI G_GGCC'C
Cuts at: 335 335
Size: 4112
ApaLI G'TGCA_C
Cuts at: 871 2882 871
Size: 2011 2101
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1063 1132 1063
Size: 69 4043
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 691 1128 1542 1790 1825 691
Size: 437 414 248 35 2978
Fragments arranged by size:
2978 437 414 248 35
BanII G_rGCy'C
Cuts at: 272 335 470 1126 2156 272
Size: 63 135 656 1030 2228
Fragments arranged by size:
2228 1030 656 135 63
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BbsI GAAGACnn'nnnn_
Cuts at: 520 520
Size: 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 701 1632 701
Size: 931 3181
BglI GCCn_nnn'nGGC
Cuts at: 964 964
Size: 4112
BglII A'GATC_T
Cuts at: 632 1627 3664 632
Size: 995 2037 1080
Fragments arranged by size:
2037 1080 995
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 335 696 875 1014 1737 1830 2886 335
Size: 361 179 139 723 93 1056 1561
Fragments arranged by size:
1561 1056 723 361 179 139 93
BmgBI CAC'GTC
Cuts at: 729 729
Size: 4112
BmrI ACTGGGnnnn_n'
Cuts at: 1324 1405 1740 3884 1324
Size: 81 335 2144 1552
Fragments arranged by size:
2144 1552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 807 2479 807
Size: 1672 2440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 760 1003 1015 1074 613
Size: 147 243 12 59 3651
Fragments arranged by size:
3651 243 147 59 12
Bpu10I CC'TnA_GC
Cuts at: 246 403 246
Size: 157 3955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2167 2659 2957 1288
Size: 879 492 298 2443
Fragments arranged by size:
2443 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1028 1060 3816 1028
Size: 32 2756 1324
Fragments arranged by size:
2756 1324 32
BsaAI yAC'GTr
Cuts at: 507 1465 507
Size: 958 3154
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 178 1543 1791 2493 3989 178
Size: 1365 248 702 1496 301
Fragments arranged by size:
1496 1365 702 301 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 272 610 646 1537 2295 2872 3368 272
Size: 338 36 891 758 577 496 1016
Fragments arranged by size:
1016 891 758 577 496 338 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 751
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 351 1654 1700 2908 104
Size: 247 1303 46 1208 1308
Fragments arranged by size:
1308 1303 1208 247 46
BsiHKAI G_wGCw'C
Cuts at: 413 875 1126 1904 2094 2886 413
Size: 462 251 778 190 792 1639
Fragments arranged by size:
1639 792 778 462 251 190
BsmI GAATG_Cn'
Cuts at: 940 967 940
Size: 27 4085
Bsp1286I G_dGCh'C
Cuts at: 272 335 413 470 696 875 1014 1126 1737
Size: 63 78 57 226 179 139 112 611
Cuts at: 1737 1830 1904 2094 2156 2886 272
Size: 93 74 190 62 730 1498
Fragments arranged by size:
1498 730 611 226 190 179 139 112
93 78 74 63 62 57
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 414 1162 1376 1678 2059 191
Size: 223 748 214 302 381 2244
Fragments arranged by size:
2244 748 381 302 223 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1035 2024 1035
Size: 989 3123
BsrFI r'CCGG_y
Cuts at: 118 2110 2291 2500 118
Size: 1992 181 209 1730
Fragments arranged by size:
1992 1730 209 181
BssSI C'ACGA_G
Cuts at: 808 2383 2741 808
Size: 1575 358 2179
Fragments arranged by size:
2179 1575 358
BstAPI GCAn_nnn'nTGC
Cuts at: 981 1489 981
Size: 508 3604
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 486 579 486
Size: 93 4019
BstYI r'GATC_y
Cuts at: 1 632 1137 1604 1627 1962 2208 3220 3306
Size: 631 505 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1012 631 505 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 224 417 107
Size: 117 193 3802
Fragments arranged by size:
3802 193 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 272 342 654 2062 2243 2406 272
Size: 70 312 1408 181 163 1978
Fragments arranged by size:
1978 1408 312 181 163 70
BtsI GCAGTG_nn'
Cuts at: 760 1014 1355 760
Size: 254 341 3517
Fragments arranged by size:
3517 341 254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 517 552 517
Size: 35 4077
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 878 914 1697 1871 2262 2289 3634 878
Size: 36 783 174 391 27 1345 1356
Fragments arranged by size:
1356 1345 783 391 174 36 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 266 1049 2320 2630 2776 3922 266
Size: 783 1271 310 146 1146 456
Fragments arranged by size:
1271 1146 783 456 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 760 930 1003 1015 1074 1937 2369 3116
Size: 147 170 73 12 59 863 432 747
Cuts at: 3116 3384 613
Size: 268 1341
Fragments arranged by size:
1341 863 747 432 268 170 147 73
59 12
EcoO109I rG'GnC_Cy
Cuts at: 203 311 331 332 825 990 1085 1251 203
Size: 108 20 1 493 165 95 166 3064
Fragments arranged by size:
3064 493 166 165 108 95 20 1
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 960 2131 960
Size: 1171 2941
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 434 600 1546 1794 2816 3795 434
Size: 166 946 248 1022 979 751
Fragments arranged by size:
1022 979 946 751 248 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 817 849 889 921 1025 1057 1954 1984 1986
Size: 32 40 32 104 32 897 30 2
Cuts at: 1986 2016 817
Size: 30 2913
Fragments arranged by size:
2913 897 104 40 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 182 1151 182
Size: 969 3143
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 181 740 2783 2967 181
Size: 559 2043 184 1326
Fragments arranged by size:
2043 1326 559 184
MscI TGG'CCA
Cuts at: 880 1873 880
Size: 993 3119
MslI CAynn'nnrTG
Cuts at: 577 727 2101 2228 577
Size: 150 1374 127 2461
Fragments arranged by size:
2461 1374 150 127
MspA1I CmG'CkG
Cuts at: 148 241 646 1223 1537 1897 2910 3155 148
Size: 93 405 577 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 577 405 360 314 245 93
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NcoI C'CATG_G
Cuts at: 107 224 107
Size: 117 3995
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NspI r_CATG'y
Cuts at: 848 1165 1665 2572 848
Size: 317 500 907 2388
Fragments arranged by size:
2388 907 500 317
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PflMI CCAn_nnn'nTGG
Cuts at: 755 755
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PpuMI rG'GwC_Cy
Cuts at: 203 311 825 990 203
Size: 108 514 165 3325
Fragments arranged by size:
3325 514 165 108
PspOMI G'GGCC_C
Cuts at: 331 331
Size: 4112
PstI C_TGCA'G
Cuts at: 989 1159 1844 989
Size: 170 685 3257
Fragments arranged by size:
3257 685 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 646 1537 1897 646
Size: 891 360 2861
Fragments arranged by size:
2861 891 360
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 180 1149 180
Size: 969 3143
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 901 3629 901
Size: 2728 1384
SfcI C'TryA_G
Cuts at: 33 621 985 1155 1840 2833 3024 33
Size: 588 364 170 685 993 191 1121
Fragments arranged by size:
1121 993 685 588 364 191 170
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SgrAI Cr'CCGG_yG
Cuts at: 118 118
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 1267 2182 2674 2936 1267
Size: 915 492 262 2443
Fragments arranged by size:
2443 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StyI C'CwwG_G
Cuts at: 107 224 479 1246 1600 107
Size: 117 255 767 354 2619
Fragments arranged by size:
2619 767 354 255 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1695 1828 2287 2496 1695
Size: 133 459 209 3311
Fragments arranged by size:
3311 459 209 133
TatI w'GTAC_w
Cuts at: 899 3627 899
Size: 2728 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 352 1909 352
Size: 1557 2555
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 576 756 1027 1607 486
Size: 90 180 271 580 2991
Fragments arranged by size:
2991 580 271 180 90
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
XmnI GAAnn'nnTTC
Cuts at: 940 940
Size: 4112
ZraI GAC'GTC
Cuts at: 179 179
Size: 4112
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI
StuI SwaI XhoI