Restriction map for
pF1KB3561
(Circular) (Six-base) MAPSORT of: pF1KB3561.seq Check: 8892 from: 1 to: 3734
pF1KB3561 3734 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 230 557 772 230
Size: 327 215 3192
Fragments arranged by size:
3192 327 215
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 623 3574 623
Size: 2951 783
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1559 1991 2738 3006 601
Size: 958 432 747 268 1329
Fragments arranged by size:
1329 958 747 432 268
AflIII A'CryG_T
Cuts at: 545 1086 2190 3167 545
Size: 541 1104 977 1112
Fragments arranged by size:
1112 1104 977 541
AgeI A'CCGG_T
Cuts at: 117 2122 117
Size: 2005 1729
AlwNI CAG_nnn'CTG
Cuts at: 309 1039 2606 309
Size: 730 1567 1437
Fragments arranged by size:
1567 1437 730
ApaLI G'TGCA_C
Cuts at: 2504 2504
Size: 3734
ApoI r'AATT_y
Cuts at: 738 3087 3343 738
Size: 2349 256 1129
Fragments arranged by size:
2349 1129 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 163 754 163
Size: 591 3143
BamHI G'GATC_C
Cuts at: 759 759
Size: 3734
BanI G'GyrC_C
Cuts at: 114 750 1164 1412 1447 114
Size: 636 414 248 35 2401
Fragments arranged by size:
2401 636 414 248 35
BanII G_rGCy'C
Cuts at: 242 346 530 748 1778 242
Size: 104 184 218 1030 2198
Fragments arranged by size:
2198 1030 218 184 104
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BbvCI CC'TCA_GC
Cuts at: 214 214
Size: 3734
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 470 1254 470
Size: 784 2950
BglI GCCn_nnn'nGGC
Cuts at: 112 461 112
Size: 349 3385
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 1359 1452 2508 1359
Size: 93 1056 2585
Fragments arranged by size:
2585 1056 93
BmgBI CAC'GTC
Cuts at: 459 459
Size: 3734
BmrI ACTGGGnnnn_n'
Cuts at: 641 946 1027 1362 3506 641
Size: 305 81 335 2144 869
Fragments arranged by size:
2144 869 335 305 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 668
Size: 3734
Bpu10I CC'TnA_GC
Cuts at: 214 214
Size: 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 546 1087 546
Size: 541 3193
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 1165 1413 2115 3611 1165
Size: 248 702 1496 1288
Fragments arranged by size:
1496 1288 702 248
BsaWI w'CCGG_w
Cuts at: 117 923 1444 2122 2396 2543 2954 117
Size: 806 521 678 274 147 411 897
Fragments arranged by size:
897 806 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 520
Size: 3734
BseYI C'CCAG_C
Cuts at: 334 616 1159 1917 2494 2990 334
Size: 282 543 758 577 496 1078
Fragments arranged by size:
1078 758 577 543 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 268
Size: 3734
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2530 104
Size: 1172 46 1208 1308
Fragments arranged by size:
1308 1208 1172 46
BsiHKAI G_wGCw'C
Cuts at: 346 665 748 1526 1716 2508 346
Size: 319 83 778 190 792 1572
Fragments arranged by size:
1572 792 778 319 190 83
Bsp1286I G_dGCh'C
Cuts at: 242 346 530 665 748 1359 1452 1526 1716
Size: 104 184 135 83 611 93 74 190
Cuts at: 1716 1778 2508 242
Size: 62 730 1468
Fragments arranged by size:
1468 730 611 190 184 135 104 93
83 74 62
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 117 1732 1913 2122 117
Size: 1615 181 209 1729
Fragments arranged by size:
1729 1615 209 181
BssSI C'ACGA_G
Cuts at: 2005 2363 2005
Size: 358 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 1111 1111
Size: 3734
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstEII G'GTnAC_C
Cuts at: 120 120
Size: 3734
BstYI r'GATC_y
Cuts at: 1 176 759 1226 1249 1584 1830 2842 2928
Size: 175 583 467 23 335 246 1012 86
Cuts at: 2928 2940 3286 1
Size: 12 346 449
Fragments arranged by size:
1012 583 467 449 346 335 246 175
86 23 12
BtgI C'CryG_G
Cuts at: 107 611 704 107
Size: 504 93 3137
Fragments arranged by size:
3137 504 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DraIII CAC_nnn'GTG
Cuts at: 546 546
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 608 1319 1493 1884 1911 3256 608
Size: 711 174 391 27 1345 1086
Fragments arranged by size:
1345 1086 711 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 344 746 344
Size: 402 3332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 668 1559 1991 2738 3006 601
Size: 67 891 432 747 268 1329
Fragments arranged by size:
1329 891 747 432 268 67
EcoNI CCTnn'n_nnAGG
Cuts at: 187 187
Size: 3734
EcoO109I rG'GnC_Cy
Cuts at: 873 873
Size: 3734
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 1168 1416 2438 3417 1168
Size: 248 1022 979 1485
Fragments arranged by size:
1485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 140 172 477 509 1576 1606 1608 1638 140
Size: 32 305 32 1067 30 2 30 2236
Fragments arranged by size:
2236 1067 305 32 32 30 30 2
HincII GTy'rAC
Cuts at: 231 773 231
Size: 542 3192
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 610 1495 610
Size: 885 2849
MslI CAynn'nnrTG
Cuts at: 1723 1850 1723
Size: 127 3607
MspA1I CmG'CkG
Cuts at: 423 845 1159 1519 2532 2777 423
Size: 422 314 360 1013 245 1380
Fragments arranged by size:
1380 1013 422 360 314 245
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NcoI C'CATG_G
Cuts at: 107 704 107
Size: 597 3137
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NspI r_CATG'y
Cuts at: 787 1287 2194 787
Size: 500 907 2327
Fragments arranged by size:
2327 907 500
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PflMI CCAn_nnn'nTGG
Cuts at: 363 705 363
Size: 342 3392
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PstI C_TGCA'G
Cuts at: 314 781 1466 314
Size: 467 685 2582
Fragments arranged by size:
2582 685 467
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 346 748 346
Size: 402 3332
SalI G'TCGA_C
Cuts at: 229 771 229
Size: 542 3192
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 310 777 1462 2455 2646 33
Size: 277 467 685 993 191 1121
Fragments arranged by size:
1121 993 685 467 277 191
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 165 756 165
Size: 591 3143
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StyI C'CwwG_G
Cuts at: 107 704 868 1222 107
Size: 597 164 354 2619
Fragments arranged by size:
2619 597 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 326 1317 1450 1909 2118 326
Size: 991 133 459 209 1942
Fragments arranged by size:
1942 991 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3734
XmaI C'CCGG_G
Cuts at: 163 754 163
Size: 591 3143
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI