Restriction map for
pF1KB3567
(Circular) (Six-base) MAPSORT of: pF1KB3567.seq Check: 7914 from: 1 to: 5255
pF1KB3567 5255 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 939 939
Size: 5255
AccI GT'mk_AC
Cuts at: 1442 2117 2293 1442
Size: 675 176 4404
Fragments arranged by size:
4404 675 176
Acc65I G'GTAC_C
Cuts at: 297 2271 297
Size: 1974 3281
AclI AA'CG_TT
Cuts at: 5095 5095
Size: 5255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1743 1845 3080 3512 3865 4527 1743
Size: 102 1235 432 353 662 2471
Fragments arranged by size:
2471 1235 662 432 353 102
AflIII A'CryG_T
Cuts at: 2607 4407 4688 2607
Size: 1800 281 3174
Fragments arranged by size:
3174 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1347 1347
Size: 5255
AleI CACnn'nnGTG
Cuts at: 130 145 130
Size: 15 5240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1381 1381
Size: 5255
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1090 1122 1090
Size: 32 5223
AlwNI CAG_nnn'CTG
Cuts at: 309 2560 3998 309
Size: 2251 1438 1566
Fragments arranged by size:
2251 1566 1438
ApaLI G'TGCA_C
Cuts at: 640 4093 640
Size: 3453 1802
ApoI r'AATT_y
Cuts at: 2259 4608 4864 2259
Size: 2349 256 2650
Fragments arranged by size:
2650 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5255
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5255
AvaI C'yCGr_G
Cuts at: 1517 1579 2275 1517
Size: 62 696 4497
Fragments arranged by size:
4497 696 62
BamHI G'GATC_C
Cuts at: 2280 2280
Size: 5255
BanI G'GyrC_C
Cuts at: 297 1225 1596 2271 2685 2933 2968 297
Size: 928 371 675 414 248 35 2584
Fragments arranged by size:
2584 928 675 414 371 248 35
BanII G_rGCy'C
Cuts at: 844 1616 2081 2105 2269 3299 844
Size: 772 465 24 164 1030 2800
Fragments arranged by size:
2800 1030 772 465 164 24
BbeI G_GCGC'C
Cuts at: 2689 2937 2689
Size: 248 5007
BciVI GTATCCnnnnn_n'
Cuts at: 3153 4209 5255 3153
Size: 1056 1046 3153
Fragments arranged by size:
3153 1056 1046
BclI T'GATC_A
Cuts at: 2775 2775
Size: 5255
BglI GCCn_nnn'nGGC
Cuts at: 1409 1409
Size: 5255
BglII A'GATC_T
Cuts at: 1154 2190 2770 4807 1154
Size: 1036 580 2037 1602
Fragments arranged by size:
2037 1602 1036 580
BlpI GC'TnA_GC
Cuts at: 2367 2367
Size: 5255
Bme1580I G_kGCm'C
Cuts at: 644 1601 2880 2973 4097 644
Size: 957 1279 93 1124 1802
Fragments arranged by size:
1802 1279 1124 957 93
BmgBI CAC'GTC
Cuts at: 591 591
Size: 5255
BmrI ACTGGGnnnn_n'
Cuts at: 152 387 451 794 2467 2548 2883 5027 152
Size: 235 64 343 1673 81 335 2144 380
Fragments arranged by size:
2144 1673 380 343 335 235 81 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 858 3622 858
Size: 2764 2491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 853 855 1047 1171 687
Size: 166 2 192 124 4771
Fragments arranged by size:
4771 192 166 124 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 808 1165 2431 3310 4024 4322 700
Size: 108 357 1266 879 714 298 1633
Fragments arranged by size:
1633 1266 879 714 357 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 2149 4959 2149
Size: 2810 2445
BsaAI yAC'GTr
Cuts at: 2608 2608
Size: 5255
BsaBI GATnn'nnATC
Cuts at: 6 2769 4806 6
Size: 2763 2037 455
Fragments arranged by size:
2763 2037 455
BsaHI Gr'CG_yC
Cuts at: 1175 2686 2934 3636 5132 1175
Size: 1511 248 702 1496 1298
Fragments arranged by size:
1511 1496 1298 702 248
BsaWI w'CCGG_w
Cuts at: 983 1455 1958 1979 2444 2965 3643 4054 4201
Size: 472 503 21 465 521 678 411 147
Cuts at: 4201 4475 983
Size: 274 1763
Fragments arranged by size:
1763 678 521 503 472 465 411 274
147 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 1029 1744 130
Size: 899 715 3641
Fragments arranged by size:
3641 899 715
BseYI C'CCAG_C
Cuts at: 183 849 1269 1663 1920 2680 3438 4103 4511
Size: 666 420 394 257 760 758 665 408
Cuts at: 4511 183
Size: 927
Fragments arranged by size:
927 760 758 666 665 420 408 394
257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 181 484 123
Size: 58 303 4894
Fragments arranged by size:
4894 303 58
BsiEI CG_ry'CG
Cuts at: 104 1413 1478 2117 2797 2843 4073 104
Size: 1309 65 639 680 46 1230 1286
Fragments arranged by size:
1309 1286 1230 680 639 65 46
BsiHKAI G_wGCw'C
Cuts at: 441 644 1246 1370 1721 2081 2269 3047 3237
Size: 203 602 124 351 360 188 778 190
Cuts at: 3237 4097 441
Size: 860 1599
Fragments arranged by size:
1599 860 778 602 360 351 203 190
188 124
BsmI GAATG_Cn'
Cuts at: 210 210
Size: 5255
BsmBI CGTCTCn'nnnn_
Cuts at: 1309 1520 1309
Size: 211 5044
Bsp1286I G_dGCh'C
Cuts at: 441 644 844 1246 1370 1601 1616 1721 2081
Size: 203 200 402 124 231 15 105 360
Cuts at: 2081 2105 2269 2880 2973 3047 3237 3299 4097
Size: 24 164 611 93 74 190 62 798
Cuts at: 4097 441
Size: 1599
Fragments arranged by size:
1599 798 611 402 360 231 203 200
190 164 124 105 93 74 62 24
15
BspEI T'CCGG_A
Cuts at: 983 1455 1958 1979 983
Size: 472 503 21 4259
Fragments arranged by size:
4259 503 472 21
BspHI T'CATG_A
Cuts at: 3687 3687
Size: 5255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 614 939 1553 1853 2305 2519 2821 3202 614
Size: 325 614 300 452 214 302 381 2667
Fragments arranged by size:
2667 614 452 381 325 302 300 214
BsrBI CCG'CTC
Cuts at: 50 837 3547 3601 5085 5250 50
Size: 787 2710 54 1484 165 55
Fragments arranged by size:
2710 1484 787 165 55 54
BsrDI GCAATG_nn'
Cuts at: 467 1917 3167 467
Size: 1450 1250 2555
Fragments arranged by size:
2555 1450 1250
BsrFI r'CCGG_y
Cuts at: 129 544 1473 3253 3434 129
Size: 415 929 1780 181 1950
Fragments arranged by size:
1950 1780 929 415 181
BssHII G'CGCG_C
Cuts at: 1522 1522
Size: 5255
BssSI C'ACGA_G
Cuts at: 3526 4234 3526
Size: 708 4547
BstAPI GCAn_nnn'nTGC
Cuts at: 2632 2632
Size: 5255
BstBI TT'CG_AA
Cuts at: 3616 3616
Size: 5255
BstEII G'GTnAC_C
Cuts at: 768 768
Size: 5255
BstXI CCAn_nnnn'nTGG
Cuts at: 183 183
Size: 5255
BstYI r'GATC_y
Cuts at: 1 1154 1301 1688 2190 2280 2747 2770 3105
Size: 1153 147 387 502 90 467 23 335
Cuts at: 3105 3351 3657 3669 3755 4807 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1153 1052 502 467 449 387 335 306
246 147 90 86 23 12
BtgI C'CryG_G
Cuts at: 371 1247 371
Size: 876 4379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 3205 3386 3549 264
Size: 2941 181 163 1970
Fragments arranged by size:
2941 1970 181 163
BtsI GCAGTG_nn'
Cuts at: 2498 2498
Size: 5255
DraI TTT'AAA
Cuts at: 2254 2254
Size: 5255
DraIII CAC_nnn'GTG
Cuts at: 507 507
Size: 5255
DrdI GACnn_nn'nnGTC
Cuts at: 2113 2961 4305 2113
Size: 848 1344 3063
Fragments arranged by size:
3063 1344 848
EaeI y'GGCC_r
Cuts at: 464 519 1410 1475 1505 2033 2840 3014 3405
Size: 55 891 65 30 528 807 174 391
Cuts at: 3405 3432 4777 464
Size: 27 1345 942
Fragments arranged by size:
1345 942 891 807 528 391 174 65
55 30 27
EagI C'GGCC_G
Cuts at: 1410 1475 2840 1410
Size: 65 1365 3825
Fragments arranged by size:
3825 1365 65
EarI CTCTTCn'nnn_
Cuts at: 277 1651 1721 1762 3278 3488 277
Size: 1374 70 41 1516 210 2044
Fragments arranged by size:
2044 1516 1374 210 70 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1558 3463 4205 4351 5065 1558
Size: 1905 742 146 714 1748
Fragments arranged by size:
1905 1748 742 714 146
EcoICRI GAG'CTC
Cuts at: 2079 2267 2079
Size: 188 5067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 853 855 1047 1171 1743 1845 3080 3512
Size: 166 2 192 124 572 102 1235 432
Cuts at: 3512 3865 4527 687
Size: 353 662 1415
Fragments arranged by size:
1415 1235 662 572 432 353 192 166
124 102 2
EcoNI CCTnn'n_nnAGG
Cuts at: 223 1549 223
Size: 1326 3929
EcoO109I rG'GnC_Cy
Cuts at: 266 321 328 717 1449 1752 2394 266
Size: 55 7 389 732 303 642 3127
Fragments arranged by size:
3127 732 642 389 303 55 7
EcoRI G'AATT_C
Cuts at: 2259 2259
Size: 5255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1512 1773 3274 1512
Size: 261 1501 3493
Fragments arranged by size:
3493 1501 261
FspI TGC'GCA
Cuts at: 790 3036 790
Size: 2246 3009
HaeII r_GCGC'y
Cuts at: 674 832 1404 2689 2937 4167 4938 674
Size: 158 572 1285 248 1230 771 991
Fragments arranged by size:
1285 1230 991 771 572 248 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 738 1024 1056 1333 1365 3097 3127 3129
Size: 32 286 32 277 32 1732 30 2
Cuts at: 3129 3159 706
Size: 30 2802
Fragments arranged by size:
2802 1732 286 277 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2118 2294 2118
Size: 176 5079
KasI G'GCGC_C
Cuts at: 2685 2933 2685
Size: 248 5007
KpnI G_GTAC'C
Cuts at: 301 2275 301
Size: 1974 3281
MluI A'CGCG_T
Cuts at: 4688 4688
Size: 5255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 4014 4198 92
Size: 3922 184 1149
Fragments arranged by size:
3922 1149 184
MscI TGG'CCA
Cuts at: 1507 2035 3016 1507
Size: 528 981 3746
Fragments arranged by size:
3746 981 528
MslI CAynn'nnrTG
Cuts at: 130 145 203 3244 3371 130
Size: 15 58 3041 127 2014
Fragments arranged by size:
3041 2014 127 58 15
MspA1I CmG'CkG
Cuts at: 171 240 564 1249 1767 2235 2366 2680 3040
Size: 69 324 685 518 468 131 314 360
Cuts at: 3040 3824 4069 171
Size: 784 245 1357
Fragments arranged by size:
1357 784 685 518 468 360 324 314
245 131 69
NaeI GCC'GGC
Cuts at: 546 1475 3436 546
Size: 929 1961 2365
Fragments arranged by size:
2365 1961 929
NarI GG'CG_CC
Cuts at: 2686 2934 2686
Size: 248 5007
NdeI CA'TA_TG
Cuts at: 1065 1065
Size: 5255
NgoMIV G'CCGG_C
Cuts at: 544 1473 3434 544
Size: 929 1961 2365
Fragments arranged by size:
2365 1961 929
NspI r_CATG'y
Cuts at: 2308 2808 4411 2308
Size: 500 1603 3152
Fragments arranged by size:
3152 1603 500
PasI CC'CwG_GG
Cuts at: 998 998
Size: 5255
PciI A'CATG_T
Cuts at: 4407 4407
Size: 5255
PflMI CCAn_nnn'nTGG
Cuts at: 309 1280 309
Size: 971 4284
PmeI GTTT'AAAC
Cuts at: 2254 2254
Size: 5255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2178 3701 3733 2146
Size: 32 1523 32 3668
Fragments arranged by size:
3668 1523 32 32
PpuMI rG'GwC_Cy
Cuts at: 266 321 328 1449 266
Size: 55 7 1121 4072
Fragments arranged by size:
4072 1121 55 7
PshAI GACnn'nnGTC
Cuts at: 326 326
Size: 5255
PstI C_TGCA'G
Cuts at: 260 1360 2302 2987 260
Size: 1100 942 685 2528
Fragments arranged by size:
2528 1100 942 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5255
PvuII CAG'CTG
Cuts at: 240 564 1767 2235 2680 3040 240
Size: 324 1203 468 445 360 2455
Fragments arranged by size:
2455 1203 468 445 360 324
RsrII CG'GwC_CG
Cuts at: 3450 3450
Size: 5255
SacI G_AGCT'C
Cuts at: 2081 2269 2081
Size: 188 5067
SacII CC_GC'GG
Cuts at: 1250 1250
Size: 5255
SalI G'TCGA_C
Cuts at: 2116 2292 2116
Size: 176 5079
SanDI GG'GwC_CC
Cuts at: 266 328 266
Size: 62 5193
SapI GCTCTTCn'nnn_
Cuts at: 3278 3488 3278
Size: 210 5045
SbfI CC_TGCA'GG
Cuts at: 2302 2302
Size: 5255
ScaI AGT'ACT
Cuts at: 4772 4772
Size: 5255
SfcI C'TryA_G
Cuts at: 33 256 1356 2298 2983 3951 4142 33
Size: 223 1100 942 685 968 191 1146
Fragments arranged by size:
1146 1100 968 942 685 223 191
SfoI GGC'GCC
Cuts at: 2687 2935 2687
Size: 248 5007
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5255
SgrAI Cr'CCGG_yG
Cuts at: 129 129
Size: 5255
SmaI CCC'GGG
Cuts at: 1581 2277 1581
Size: 696 4559
SmlI C'TyrA_G
Cuts at: 679 823 1180 2410 3325 4039 4301 679
Size: 144 357 1230 915 714 262 1633
Fragments arranged by size:
1633 1230 915 714 357 262 144
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5255
SphI G_CATG'C
Cuts at: 2308 2808 2308
Size: 500 4755
StuI AGG'CCT
Cuts at: 286 688 286
Size: 402 4853
StyI C'CwwG_G
Cuts at: 695 1853 2389 2743 695
Size: 1158 536 354 3207
Fragments arranged by size:
3207 1158 536 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 573 1272 2838 2971 3430 3639 573
Size: 699 1566 133 459 209 2189
Fragments arranged by size:
2189 1566 699 459 209 133
TatI w'GTAC_w
Cuts at: 134 4770 134
Size: 4636 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4772 4804 4772
Size: 32 5223
Tth111I GACn'n_nGTC
Cuts at: 592 3052 592
Size: 2460 2795
XbaI T'CTAG_A
Cuts at: 879 2286 879
Size: 1407 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 1277 2750 216
Size: 1061 1473 2721
Fragments arranged by size:
2721 1473 1061
XmaI C'CCGG_G
Cuts at: 1579 2275 1579
Size: 696 4559
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsrGI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PfoI PmlI PsiI PspOMI PspXI PsrI SexAI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI