Restriction map for
pF1KB3580
(Circular) (Six-base) MAPSORT of: pF1KB3580.seq Check: 1640 from: 1 to: 4727
pF1KB3580 4727 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 571 2552 2984 3337 3999 557
Size: 14 1981 432 353 662 1285
Fragments arranged by size:
1981 1285 662 432 353 14
AflIII A'CryG_T
Cuts at: 2079 3879 4160 2079
Size: 1800 281 2646
Fragments arranged by size:
2646 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 616 2032 3470 616
Size: 1416 1438 1873
Fragments arranged by size:
1873 1438 1416
ApaI G_GGCC'C
Cuts at: 939 939
Size: 4727
ApaLI G'TGCA_C
Cuts at: 3565 3565
Size: 4727
ApoI r'AATT_y
Cuts at: 421 579 850 971 1285 1731 4080 4336 421
Size: 158 271 121 314 446 2349 256 812
Fragments arranged by size:
2349 812 446 314 271 256 158 121
AseI AT'TA_AT
Cuts at: 20 323 20
Size: 303 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 1747 1747
Size: 4727
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 339 1743 2157 2405 2440 339
Size: 1404 414 248 35 2626
Fragments arranged by size:
2626 1404 414 248 35
BanII G_rGCy'C
Cuts at: 684 939 1741 2771 684
Size: 255 802 1030 2640
Fragments arranged by size:
2640 1030 802 255
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbsI GAAGACnn'nnnn_
Cuts at: 1640 1640
Size: 4727
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3681 4727 2625
Size: 1056 1046 2625
Fragments arranged by size:
2625 1056 1046
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BfrBI ATG'CAT
Cuts at: 1026 1026
Size: 4727
BglI GCCn_nnn'nGGC
Cuts at: 934 934
Size: 4727
BglII A'GATC_T
Cuts at: 1580 2242 4279 1580
Size: 662 2037 2028
Fragments arranged by size:
2037 2028 662
BlpI GC'TnA_GC
Cuts at: 640 1119 1839 640
Size: 479 720 3528
Fragments arranged by size:
3528 720 479
Bme1580I G_kGCm'C
Cuts at: 623 939 1221 2352 2445 3569 623
Size: 316 282 1131 93 1124 1781
Fragments arranged by size:
1781 1131 1124 316 282 93
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1939 2020 2355 4499 1381
Size: 558 81 335 2144 1609
Fragments arranged by size:
2144 1609 558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 4727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 830 1355 719
Size: 111 525 4091
Fragments arranged by size:
4091 525 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 2782 3496 3794 1903
Size: 879 714 298 2836
Fragments arranged by size:
2836 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4431 4431
Size: 4727
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 2158 2406 3108 4604 2158
Size: 248 702 1496 2281
Fragments arranged by size:
2281 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1916 2437 3115 3526 3673 3947 1916
Size: 521 678 411 147 274 2696
Fragments arranged by size:
2696 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 1390 201
Size: 1189 3538
BseYI C'CCAG_C
Cuts at: 932 1254 2152 2910 3575 3983 932
Size: 322 898 758 665 408 1676
Fragments arranged by size:
1676 898 758 665 408 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 735
Size: 4727
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3545 104
Size: 2165 46 1230 1286
Fragments arranged by size:
2165 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1741 2519 2709 3569 1741
Size: 778 190 860 2899
Fragments arranged by size:
2899 860 778 190
BsmI GAATG_Cn'
Cuts at: 804 804
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 623 684 939 1221 1741 2352 2445 2519 2709
Size: 61 255 282 520 611 93 74 190
Cuts at: 2709 2771 3569 623
Size: 62 798 1781
Fragments arranged by size:
1781 798 611 520 282 255 190 93
74 62 61
BspHI T'CATG_A
Cuts at: 309 1566 3159 309
Size: 1257 1593 1877
Fragments arranged by size:
1877 1593 1257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1777 1991 2293 2674 1777
Size: 214 302 381 3830
Fragments arranged by size:
3830 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1389 3019 3073 4557 4722 50
Size: 1339 1630 54 1484 165 55
Fragments arranged by size:
1630 1484 1339 165 55 54
BsrDI GCAATG_nn'
Cuts at: 692 1643 2639 692
Size: 951 996 2780
Fragments arranged by size:
2780 996 951
BsrFI r'CCGG_y
Cuts at: 2725 2906 2725
Size: 181 4546
BsrGI T'GTAC_A
Cuts at: 529 1425 529
Size: 896 3831
BssSI C'ACGA_G
Cuts at: 2998 3706 2998
Size: 708 4019
BstAPI GCAn_nnn'nTGC
Cuts at: 2104 2104
Size: 4727
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstXI CCAn_nnnn'nTGG
Cuts at: 501 501
Size: 4727
BstYI r'GATC_y
Cuts at: 1 1223 1580 1752 2219 2242 2577 2823 3129
Size: 1222 357 172 467 23 335 246 306
Cuts at: 3129 3141 3227 4279 1
Size: 12 86 1052 449
Fragments arranged by size:
1222 1052 467 449 357 335 306 246
172 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1459 1459
Size: 4727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 532 1970 532
Size: 1438 3289
ClaI AT'CG_AT
Cuts at: 986 986
Size: 4727
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DraIII CAC_nnn'GTG
Cuts at: 346 346
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3777 2433
Size: 1344 3383
EaeI y'GGCC_r
Cuts at: 491 2312 2486 2877 2904 4249 491
Size: 1821 174 391 27 1345 969
Fragments arranged by size:
1821 1345 969 391 174 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 224 590 2750 2960 224
Size: 366 2160 210 1991
Fragments arranged by size:
2160 1991 366 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3677 3823 4537 2935
Size: 742 146 714 3125
Fragments arranged by size:
3125 742 714 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 571 719 830 1355 2552 2984 3337 3999
Size: 14 148 111 525 1197 432 353 662
Cuts at: 3999 557
Size: 1285
Fragments arranged by size:
1285 1197 662 525 432 353 148 111
14
EcoNI CCTnn'n_nnAGG
Cuts at: 1706 1706
Size: 4727
EcoO109I rG'GnC_Cy
Cuts at: 936 1866 936
Size: 930 3797
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
EcoRV GAT'ATC
Cuts at: 1193 1193
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 181 2508 181
Size: 2327 2400
HaeII r_GCGC'y
Cuts at: 2161 2409 3639 4410 2161
Size: 248 1230 771 2478
Fragments arranged by size:
2478 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2569 2599 2601 2631 2569
Size: 30 2 30 4665
Fragments arranged by size:
4665 30 30 2
HincII GTy'rAC
Cuts at: 1659 1766 1659
Size: 107 4620
HindIII A'AGCT_T
Cuts at: 1529 1529
Size: 4727
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1710 3486 3670 1165
Size: 545 1776 184 2222
Fragments arranged by size:
2222 1776 545 184
MscI TGG'CCA
Cuts at: 493 2488 493
Size: 1995 2732
MslI CAynn'nnrTG
Cuts at: 334 1408 2716 2843 334
Size: 1074 1308 127 2218
Fragments arranged by size:
2218 1308 1074 127
MspA1I CmG'CkG
Cuts at: 1207 1345 1838 2152 2512 3296 3541 1207
Size: 138 493 314 360 784 245 2393
Fragments arranged by size:
2393 784 493 360 314 245 138
NaeI GCC'GGC
Cuts at: 2908 2908
Size: 4727
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NgoMIV G'CCGG_C
Cuts at: 2906 2906
Size: 4727
NsiI A_TGCA'T
Cuts at: 1028 1028
Size: 4727
NspI r_CATG'y
Cuts at: 1184 1780 2280 3883 1184
Size: 596 500 1603 2028
Fragments arranged by size:
2028 1603 596 500
PciI A'CATG_T
Cuts at: 3879 3879
Size: 4727
PfoI T'CCnGG_A
Cuts at: 662 662
Size: 4727
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4695
PsiI TTA'TAA
Cuts at: 1037 1037
Size: 4727
PspOMI G'GGCC_C
Cuts at: 935 935
Size: 4727
PstI C_TGCA'G
Cuts at: 209 539 1130 1774 2459 209
Size: 330 591 644 685 2477
Fragments arranged by size:
2477 685 644 591 330
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 1207 1345 2152 2512 1207
Size: 138 807 360 3422
Fragments arranged by size:
3422 807 360 138
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 224 2750 2960 224
Size: 2526 210 1991
Fragments arranged by size:
2526 1991 210
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SexAI A'CCwGG_T
Cuts at: 342 342
Size: 4727
SfcI C'TryA_G
Cuts at: 33 205 260 535 825 1126 1770 2455 3423
Size: 172 55 275 290 301 644 685 968
Cuts at: 3423 3614 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 644 301 290 275 191
172 55
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 1882 2797 3511 3773 1882
Size: 915 714 262 2836
Fragments arranged by size:
2836 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
SspI AAT'ATT
Cuts at: 567 1200 567
Size: 633 4094
StuI AGG'CCT
Cuts at: 1594 1594
Size: 4727
StyI C'CwwG_G
Cuts at: 1861 2215 1861
Size: 354 4373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1639 2310 2443 2902 3111 1639
Size: 671 133 459 209 3255
Fragments arranged by size:
3255 671 459 209 133
TatI w'GTAC_w
Cuts at: 529 915 1425 1676 4242 529
Size: 386 510 251 2566 1014
Fragments arranged by size:
2566 1014 510 386 251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 4695
Tth111I GACn'n_nGTC
Cuts at: 724 2524 724
Size: 1800 2927
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 4727
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BspEI BssHII BstEII BstZ17I
BtgI CspCI FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI