Restriction map for
pF1KB3596
(Circular) (Six-base) MAPSORT of: pF1KB3596.seq Check: 3268 from: 1 to: 4622
pF1KB3596 4622 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1660 1660
Size: 4622
Acc65I G'GTAC_C
Cuts at: 1072 1638 1072
Size: 566 4056
AclI AA'CG_TT
Cuts at: 825 4462 825
Size: 3637 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 2447 2879 3232 3894 1271
Size: 1176 432 353 662 1999
Fragments arranged by size:
1999 1176 662 432 353
AflIII A'CryG_T
Cuts at: 1974 3774 4055 1974
Size: 1800 281 2541
Fragments arranged by size:
2541 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 673 1000 1927 3365 673
Size: 327 927 1438 1930
Fragments arranged by size:
1930 1438 927 327
ApaLI G'TGCA_C
Cuts at: 365 3460 365
Size: 3095 1527
ApoI r'AATT_y
Cuts at: 1364 1626 3975 4231 1364
Size: 262 2349 256 1755
Fragments arranged by size:
2349 1755 262 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 192 344 1642 192
Size: 152 1298 3172
Fragments arranged by size:
3172 1298 152
BamHI G'GATC_C
Cuts at: 964 1647 964
Size: 683 3939
BanI G'GyrC_C
Cuts at: 1072 1638 2052 2300 2335 1072
Size: 566 414 248 35 3359
Fragments arranged by size:
3359 566 414 248 35
BanII G_rGCy'C
Cuts at: 266 1636 2666 266
Size: 1370 1030 2222
Fragments arranged by size:
2222 1370 1030
BbeI G_GCGC'C
Cuts at: 2056 2304 2056
Size: 248 4374
BciVI GTATCCnnnnn_n'
Cuts at: 2520 3576 4622 2520
Size: 1056 1046 2520
Fragments arranged by size:
2520 1056 1046
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4622
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 1734 1734
Size: 4622
Bme1580I G_kGCm'C
Cuts at: 369 2247 2340 3464 369
Size: 1878 93 1124 1527
Fragments arranged by size:
1878 1527 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 392 1834 1915 2250 4394 392
Size: 1442 81 335 2144 620
Fragments arranged by size:
2144 1442 620 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4622
Bpu10I CC'TnA_GC
Cuts at: 678 678
Size: 4622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1518 1798 2677 3391 3689 1518
Size: 280 879 714 298 2451
Fragments arranged by size:
2451 879 714 298 280
BsaI GGTCTCn'nnnn_
Cuts at: 280 4326 280
Size: 4046 576
BsaAI yAC'GTr
Cuts at: 1975 1975
Size: 4622
BsaBI GATnn'nnATC
Cuts at: 6 2136 4173 6
Size: 2130 2037 455
Fragments arranged by size:
2130 2037 455
BsaHI Gr'CG_yC
Cuts at: 2053 2301 3003 4499 2053
Size: 248 702 1496 2176
Fragments arranged by size:
2176 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1811 2332 3010 3421 3568 3842 1811
Size: 521 678 411 147 274 2591
Fragments arranged by size:
2591 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 776 806 776
Size: 30 4592
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 139 801 136
Size: 3 662 3957
Fragments arranged by size:
3957 662 3
BseYI C'CCAG_C
Cuts at: 2047 2805 3470 3878 2047
Size: 758 665 408 2791
Fragments arranged by size:
2791 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 122 2164 2210 3440 104
Size: 18 2042 46 1230 1286
Fragments arranged by size:
2042 1286 1230 46 18
BsiHKAI G_wGCw'C
Cuts at: 369 1636 2414 2604 3464 369
Size: 1267 778 190 860 1527
Fragments arranged by size:
1527 1267 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 266 369 1636 2247 2340 2414 2604 2666 3464
Size: 103 1267 611 93 74 190 62 798
Cuts at: 3464 266
Size: 1424
Fragments arranged by size:
1424 1267 798 611 190 103 93 74
62
BspHI T'CATG_A
Cuts at: 873 954 1368 3054 873
Size: 81 414 1686 2441
Fragments arranged by size:
2441 1686 414 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1672 1886 2188 2569 1672
Size: 214 302 381 3725
Fragments arranged by size:
3725 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1040 2914 2968 4452 4617 50
Size: 990 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 990 165 55 54
BsrDI GCAATG_nn'
Cuts at: 420 494 688 1091 1322 1561 2534 420
Size: 74 194 403 231 239 973 2508
Fragments arranged by size:
2508 973 403 239 231 194 74
BsrFI r'CCGG_y
Cuts at: 2620 2801 2620
Size: 181 4441
BsrGI T'GTAC_A
Cuts at: 229 229
Size: 4622
BssSI C'ACGA_G
Cuts at: 151 2893 3601 151
Size: 2742 708 1172
Fragments arranged by size:
2742 1172 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1999 1999
Size: 4622
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstXI CCAn_nnnn'nTGG
Cuts at: 1478 1478
Size: 4622
BstYI r'GATC_y
Cuts at: 1 964 1647 2114 2137 2472 2718 3024 3036
Size: 963 683 467 23 335 246 306 12
Cuts at: 3036 3122 4174 1
Size: 86 1052 449
Fragments arranged by size:
1052 963 683 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1031 107
Size: 924 3698
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2572 2753 2916 2572
Size: 181 163 4278
Fragments arranged by size:
4278 181 163
BtsI GCAGTG_nn'
Cuts at: 1865 1865
Size: 4622
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DraIII CAC_nnn'GTG
Cuts at: 1224 1224
Size: 4622
DrdI GACnn_nn'nnGTC
Cuts at: 2328 3672 2328
Size: 1344 3278
EaeI y'GGCC_r
Cuts at: 2207 2381 2772 2799 4144 2207
Size: 174 391 27 1345 2685
Fragments arranged by size:
2685 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2207 2207
Size: 4622
EarI CTCTTCn'nnn_
Cuts at: 378 584 2645 2855 378
Size: 206 2061 210 2145
Fragments arranged by size:
2145 2061 210 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2830 3572 3718 4432 2830
Size: 742 146 714 3020
Fragments arranged by size:
3020 742 714 146
EcoICRI GAG'CTC
Cuts at: 1634 1634
Size: 4622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 2447 2879 3232 3894 1271
Size: 1176 432 353 662 1999
Fragments arranged by size:
1999 1176 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 594 1761 594
Size: 1167 3455
EcoRI G'AATT_C
Cuts at: 1364 1626 1364
Size: 262 4360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1140 2641 1140
Size: 1501 3121
FspI TGC'GCA
Cuts at: 2403 2403
Size: 4622
HaeII r_GCGC'y
Cuts at: 187 2056 2304 3534 4305 187
Size: 1869 248 1230 771 504
Fragments arranged by size:
1869 1230 771 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 949 981 1096 1128 2464 2494 2496 2526 949
Size: 32 115 32 1336 30 2 30 3045
Fragments arranged by size:
3045 1336 115 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1597 1661 1597
Size: 64 4558
KasI G'GCGC_C
Cuts at: 2052 2300 2052
Size: 248 4374
KpnI G_GTAC'C
Cuts at: 1076 1642 1076
Size: 566 4056
MfeI C'AATT_G
Cuts at: 707 707
Size: 4622
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 466 3381 3565 466
Size: 2915 184 1523
Fragments arranged by size:
2915 1523 184
MscI TGG'CCA
Cuts at: 2383 2383
Size: 4622
MslI CAynn'nnrTG
Cuts at: 535 959 1273 1612 2611 2738 535
Size: 424 314 339 999 127 2419
Fragments arranged by size:
2419 999 424 339 314 127
MspA1I CmG'CkG
Cuts at: 490 1733 2047 2407 3191 3436 490
Size: 1243 314 360 784 245 1676
Fragments arranged by size:
1676 1243 784 360 314 245
NaeI GCC'GGC
Cuts at: 2803 2803
Size: 4622
NarI GG'CG_CC
Cuts at: 2053 2301 2053
Size: 248 4374
NcoI C'CATG_G
Cuts at: 107 107
Size: 4622
NgoMIV G'CCGG_C
Cuts at: 2801 2801
Size: 4622
NspI r_CATG'y
Cuts at: 318 1675 2175 3778 318
Size: 1357 500 1603 1162
Fragments arranged by size:
1603 1357 1162 500
PciI A'CATG_T
Cuts at: 3774 3774
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 1224 1224
Size: 4622
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 4590
PpuMI rG'GwC_Cy
Cuts at: 594 594
Size: 4622
PstI C_TGCA'G
Cuts at: 1669 2354 1669
Size: 685 3937
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 490 2047 2407 490
Size: 1557 360 2705
Fragments arranged by size:
2705 1557 360
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 1636 1636
Size: 4622
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
SbfI CC_TGCA'GG
Cuts at: 1669 1669
Size: 4622
ScaI AGT'ACT
Cuts at: 4139 4139
Size: 4622
SfcI C'TryA_G
Cuts at: 33 1665 2350 3318 3509 33
Size: 1632 685 968 191 1146
Fragments arranged by size:
1632 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2054 2302 2054
Size: 248 4374
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 194 1644 194
Size: 1450 3172
SmlI C'TyrA_G
Cuts at: 344 1497 1777 2692 3406 3668 344
Size: 1153 280 915 714 262 1298
Fragments arranged by size:
1298 1153 915 714 280 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1675 2175 1675
Size: 500 4122
StyI C'CwwG_G
Cuts at: 107 468 1077 1756 2110 107
Size: 361 609 679 354 2619
Fragments arranged by size:
2619 679 609 361 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2205 2338 2797 3006 2205
Size: 133 459 209 3821
Fragments arranged by size:
3821 459 209 133
TatI w'GTAC_w
Cuts at: 229 4137 229
Size: 3908 714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4590
Tth111I GACn'n_nGTC
Cuts at: 663 2419 663
Size: 1756 2866
XbaI T'CTAG_A
Cuts at: 814 1653 814
Size: 839 3783
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2117
Size: 4622
XhoI C'TCGA_G
Cuts at: 344 344
Size: 4622
XmaI C'CCGG_G
Cuts at: 192 1642 192
Size: 1450 3172
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BmgBI BmtI BpmI BsgI BsiWI BsmI BsmBI
BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI