Restriction map for
pF1KB3598
(Circular) (Six-base) MAPSORT of: pF1KB3598.seq Check: 1401 from: 1 to: 7610
pF1KB3598 7610 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 1794 1794
Size: 7610
AccI GT'mk_AC
Cuts at: 4648 4648
Size: 7610
Acc65I G'GTAC_C
Cuts at: 1420 3274 4626 1420
Size: 1854 1352 4404
Fragments arranged by size:
4404 1854 1352
AclI AA'CG_TT
Cuts at: 7450 7450
Size: 7610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1319 1742 1877 2489 3541 3641 3884 3943
Size: 298 423 135 612 1052 100 243 59
Cuts at: 3943 5435 5867 6614 6882 1021
Size: 1492 432 747 268 1749
Fragments arranged by size:
1749 1492 1052 747 612 432 423 298
268 243 135 100 59
AflIII A'CryG_T
Cuts at: 3991 4111 4962 6066 7043 3991
Size: 120 851 1104 977 4558
Fragments arranged by size:
4558 1104 977 851 120
AgeI A'CCGG_T
Cuts at: 5998 5998
Size: 7610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 818 818
Size: 7610
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1215 1247 1629 1661 2799 2831 1215
Size: 32 382 32 1138 32 5994
Fragments arranged by size:
5994 1138 382 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1702 2872 2890 3736 4915 6482 1702
Size: 1170 18 846 1179 1567 2830
Fragments arranged by size:
2830 1567 1179 1170 846 18
ApaI G_GGCC'C
Cuts at: 883 1334 2080 2521 3215 3745 883
Size: 451 746 441 694 530 4748
Fragments arranged by size:
4748 746 694 530 451 441
ApaLI G'TGCA_C
Cuts at: 1178 6380 1178
Size: 5202 2408
ApoI r'AATT_y
Cuts at: 423 1493 4570 4614 6963 7219 423
Size: 1070 3077 44 2349 256 814
Fragments arranged by size:
3077 2349 1070 814 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 7610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7610
AvaI C'yCGr_G
Cuts at: 1820 1939 2567 2594 2612 2911 3422 4630 1820
Size: 119 628 27 18 299 511 1208 4800
Fragments arranged by size:
4800 1208 628 511 299 119 27 18
AvrII C'CTAG_G
Cuts at: 2901 2901
Size: 7610
BamHI G'GATC_C
Cuts at: 1782 2752 4635 1782
Size: 970 1883 4757
Fragments arranged by size:
4757 1883 970
BanI G'GyrC_C
Cuts at: 1096 1393 1420 3274 3355 4626 5040 5288 5323
Size: 297 27 1854 81 1271 414 248 35
Cuts at: 5323 1096
Size: 3383
Fragments arranged by size:
3383 1854 1271 414 297 248 81 35
27
BanII G_rGCy'C
Cuts at: 883 966 984 1334 1414 2037 2080 2521 2883
Size: 83 18 350 80 623 43 441 362
Cuts at: 2883 3215 3745 4624 5654 883
Size: 332 530 879 1030 2839
Fragments arranged by size:
2839 1030 879 623 530 441 362 350
332 83 80 43 18
BbeI G_GCGC'C
Cuts at: 5044 5292 5044
Size: 248 7362
BbsI GAAGACnn'nnnn_
Cuts at: 200 564 982 1351 2377 2494 2926 3547 3556
Size: 364 418 369 1026 117 432 621 9
Cuts at: 3556 200
Size: 4254
Fragments arranged by size:
4254 1026 621 432 418 369 364 117
9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1107 1141 1107
Size: 34 7576
BciVI GTATCCnnnnn_n'
Cuts at: 1217 4330 5508 6269 7610 1217
Size: 3113 1178 761 1341 1217
Fragments arranged by size:
3113 1341 1217 1178 761
BclI T'GATC_A
Cuts at: 2187 3501 5130 2187
Size: 1314 1629 4667
Fragments arranged by size:
4667 1629 1314
BglI GCCn_nnn'nGGC
Cuts at: 112 1409 1958 2336 2588 3137 3542 3687 3767
Size: 1297 549 378 252 549 405 145 80
Cuts at: 3767 3965 112
Size: 198 3757
Fragments arranged by size:
3757 1297 549 549 405 378 252 198
145 80
BglII A'GATC_T
Cuts at: 287 4533 5125 7162 287
Size: 4246 592 2037 735
Fragments arranged by size:
4246 2037 735 592
BlpI GC'TnA_GC
Cuts at: 1440 4722 1440
Size: 3282 4328
Bme1580I G_kGCm'C
Cuts at: 883 1182 1334 2080 2521 3215 3360 3745 3824
Size: 299 152 746 441 694 145 385 79
Cuts at: 3824 5235 5328 6384 883
Size: 1411 93 1056 2109
Fragments arranged by size:
2109 1411 1056 746 694 441 385 299
152 145 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 129 1337 3640 4822 4903 5238 7382 129
Size: 1208 2303 1182 81 335 2144 357
Fragments arranged by size:
2303 2144 1208 1182 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 331 2051 5977 331
Size: 1720 3926 1964
Fragments arranged by size:
3926 1964 1720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 500 608 1337 1445 1634 1895 2015 2033
Size: 72 108 729 108 189 261 120 18
Cuts at: 2033 2102 2138 2186 2222 2330 2384 2507 2564
Size: 69 36 48 36 108 54 123 57
Cuts at: 2564 2654 2699 2717 2849 2867 2897 2912 3371
Size: 90 45 18 132 18 30 15 459
Cuts at: 3371 3623 3686 428
Size: 252 63 4352
Fragments arranged by size:
4352 729 459 261 252 189 132 123
120 108 108 108 90 72 69 63
57 54 48 45 36 36 30 18
18 18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1715 4786 5665 6157 6455 687
Size: 1028 3071 879 492 298 1842
Fragments arranged by size:
3071 1842 1028 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3314 3503 4220 4226 7314 3314
Size: 189 717 6 3088 3610
Fragments arranged by size:
3610 3088 717 189 6
BsaAI yAC'GTr
Cuts at: 490 868 3992 4144 4963 490
Size: 378 3124 152 819 3137
Fragments arranged by size:
3137 3124 819 378 152
BsaBI GATnn'nnATC
Cuts at: 6 5124 7161 6
Size: 5118 2037 455
Fragments arranged by size:
5118 2037 455
BsaHI Gr'CG_yC
Cuts at: 1791 5041 5289 5991 7487 1791
Size: 3250 248 702 1496 1914
Fragments arranged by size:
3250 1914 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4430 4799 5320 5998 6272 6419 6830 4430
Size: 369 521 678 274 147 411 5210
Fragments arranged by size:
5210 678 521 411 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 804 834 1398 1428 804
Size: 30 564 30 6986
Fragments arranged by size:
6986 564 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 551 2117 2252 2279 3422 130
Size: 421 1566 135 27 1143 4318
Fragments arranged by size:
4318 1566 1143 421 135 27
BseYI C'CCAG_C
Cuts at: 694 3141 3451 3981 5035 5793 6370 6866 694
Size: 2447 310 530 1054 758 577 496 1438
Fragments arranged by size:
2447 1438 1054 758 577 530 496 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 824 213
Size: 611 6999
BsiEI CG_ry'CG
Cuts at: 104 3074 5152 5198 6406 104
Size: 2970 2078 46 1208 1308
Fragments arranged by size:
2970 2078 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 624 966 1056 1164 1182 1677 1731 1821 2037
Size: 342 90 108 18 495 54 90 216
Cuts at: 2037 2883 3387 4624 5402 5592 6384 624
Size: 846 504 1237 778 190 792 1850
Fragments arranged by size:
1850 1237 846 792 778 504 495 342
216 190 108 90 90 54 18
BsmI GAATG_Cn'
Cuts at: 337 385 1130 337
Size: 48 745 6817
Fragments arranged by size:
6817 745 48
Bsp1286I G_dGCh'C
Cuts at: 624 883 966 984 1056 1164 1182 1334 1414
Size: 259 83 18 72 108 18 152 80
Cuts at: 1414 1677 1731 1821 2037 2080 2521 2883 3215
Size: 263 54 90 216 43 441 362 332
Cuts at: 3215 3360 3387 3745 3824 4624 5235 5328 5402
Size: 145 27 358 79 800 611 93 74
Cuts at: 5402 5592 5654 6384 624
Size: 190 62 730 1850
Fragments arranged by size:
1850 800 730 611 441 362 358 332
263 259 216 190 152 145 108 93
90 83 80 79 74 72 62 54
43 27 18 18
BspEI T'CCGG_A
Cuts at: 6830 6830
Size: 7610
BspHI T'CATG_A
Cuts at: 6786 6786
Size: 7610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 282 1264 2524 2898 3325 4660 4874 5176 5557
Size: 982 1260 374 427 1335 214 302 381
Cuts at: 5557 282
Size: 2335
Fragments arranged by size:
2335 1335 1260 982 427 381 374 302
214
BsrBI CCG'CTC
Cuts at: 50 5902 5956 7440 7605 50
Size: 5852 54 1484 165 55
Fragments arranged by size:
5852 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3273 5522 3273
Size: 2249 5361
BsrFI r'CCGG_y
Cuts at: 504 5608 5789 5998 504
Size: 5104 181 209 2116
Fragments arranged by size:
5104 2116 209 181
BssSI C'ACGA_G
Cuts at: 569 965 3485 5881 6239 569
Size: 396 2520 2396 358 1940
Fragments arranged by size:
2520 2396 1940 396 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2008 4987 2008
Size: 2979 4631
BstBI TT'CG_AA
Cuts at: 5971 5971
Size: 7610
BstEII G'GTnAC_C
Cuts at: 1006 1627 1978 1006
Size: 621 351 6638
Fragments arranged by size:
6638 621 351
BstYI r'GATC_y
Cuts at: 1 287 952 1456 1782 2205 2752 3411 3843
Size: 286 665 504 326 423 547 659 432
Cuts at: 3843 4060 4533 4635 5102 5125 5460 5706 6718
Size: 217 473 102 467 23 335 246 1012
Cuts at: 6718 6804 6816 7162 1
Size: 86 12 346 449
Fragments arranged by size:
1012 665 659 547 504 473 467 449
432 423 346 335 326 286 246 217
102 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 578 1307 1469 1487 3737 578
Size: 729 162 18 2250 4451
Fragments arranged by size:
4451 2250 729 162 18
BtgI C'CryG_G
Cuts at: 1100 1100
Size: 7610
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5560 5741 5904 5560
Size: 181 163 7266
Fragments arranged by size:
7266 181 163
BtsI GCAGTG_nn'
Cuts at: 1980 3723 4853 1980
Size: 1743 1130 4737
Fragments arranged by size:
4737 1743 1130
DraI TTT'AAA
Cuts at: 4609 4609
Size: 7610
DraIII CAC_nnn'GTG
Cuts at: 1159 1600 1159
Size: 441 7169
DrdI GACnn_nn'nnGTC
Cuts at: 722 851 1022 1211 2660 2930 3254 3443 5316
Size: 129 171 189 1449 270 324 189 1873
Cuts at: 5316 6174 722
Size: 858 2158
Fragments arranged by size:
2158 1873 1449 858 324 270 189 189
171 129
EaeI y'GGCC_r
Cuts at: 240 1896 3786 5195 5369 5760 5787 7132 240
Size: 1656 1890 1409 174 391 27 1345 718
Fragments arranged by size:
1890 1656 1409 1345 718 391 174 27
EagI C'GGCC_G
Cuts at: 5195 5195
Size: 7610
EarI CTCTTCn'nnn_
Cuts at: 5633 5843 5633
Size: 210 7400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 2807 5818 6128 6274 7420 139
Size: 2668 3011 310 146 1146 329
Fragments arranged by size:
3011 2668 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 964 2035 2881 4622 964
Size: 1071 846 1741 3952
Fragments arranged by size:
3952 1741 1071 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 500 608 1021 1319 1337 1445 1634 1742
Size: 72 108 413 298 18 108 189 108
Cuts at: 1742 1877 1895 2015 2033 2102 2138 2186 2222
Size: 135 18 120 18 69 36 48 36
Cuts at: 2222 2330 2384 2489 2507 2564 2654 2699 2717
Size: 108 54 105 18 57 90 45 18
Cuts at: 2717 2849 2867 2897 2912 3371 3541 3623 3641
Size: 132 18 30 15 459 170 82 18
Cuts at: 3641 3686 3884 3943 5435 5867 6614 6882 428
Size: 45 198 59 1492 432 747 268 1156
Fragments arranged by size:
1492 1156 747 459 432 413 298 268
198 189 170 135 132 120 108 108
108 108 105 90 82 72 69 59
57 54 48 45 45 36 36 30
18 18 18 18 18 18 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 4055 2168
Size: 1887 5723
EcoO109I rG'GnC_Cy
Cuts at: 511 628 655 754 808 817 879 880 1033
Size: 117 27 99 54 9 62 1 153
Cuts at: 1033 1105 1213 1276 1294 1303 1331 1447 1465
Size: 72 108 63 18 9 28 116 18
Cuts at: 1465 1618 1744 1762 1852 1906 1960 2113 2122
Size: 153 126 18 90 54 54 153 9
Cuts at: 2122 2158 2230 2357 2509 2518 2536 2662 2869
Size: 36 72 127 152 9 18 126 207
Cuts at: 2869 2887 2896 2923 2943 3015 3211 3212 3427
Size: 18 9 27 20 72 196 1 215
Cuts at: 3427 3481 3634 3733 3741 3742 3778 4749 511
Size: 54 153 99 8 1 36 971 3372
Fragments arranged by size:
3372 971 215 207 196 153 153 153
153 152 127 126 126 117 116 108
99 99 90 72 72 72 63 62
54 54 54 54 36 36 28 27
27 20 18 18 18 18 18 9
9 9 9 9 8 1 1 1
EcoRI G'AATT_C
Cuts at: 1493 4570 4614 1493
Size: 3077 44 4489
Fragments arranged by size:
4489 3077 44
EcoRV GAT'ATC
Cuts at: 3813 4404 3813
Size: 591 7019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1882 4506 5629 1882
Size: 2624 1123 3863
Fragments arranged by size:
3863 2624 1123
FspI TGC'GCA
Cuts at: 3955 5391 3955
Size: 1436 6174
HaeII r_GCGC'y
Cuts at: 5044 5292 6314 7293 5044
Size: 248 1022 979 5361
Fragments arranged by size:
5361 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 694 726 1821 1853 2935 2967 4225
Size: 32 537 32 1095 32 1082 32 1258
Cuts at: 4225 4257 4441 4473 5452 5482 5484 5514 125
Size: 32 184 32 979 30 2 30 2221
Fragments arranged by size:
2221 1258 1095 1082 979 537 184 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 613 4649 613
Size: 4036 3574
HindIII A'AGCT_T
Cuts at: 4006 4006
Size: 7610
KasI G'GCGC_C
Cuts at: 5040 5288 5040
Size: 248 7362
KpnI G_GTAC'C
Cuts at: 1424 3278 4630 1424
Size: 1854 1352 4404
Fragments arranged by size:
4404 1854 1352
MfeI C'AATT_G
Cuts at: 3692 3692
Size: 7610
MluI A'CGCG_T
Cuts at: 7043 7043
Size: 7610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 680 1029 2838 3279 6281 6465 680
Size: 349 1809 441 3002 184 1825
Fragments arranged by size:
3002 1825 1809 441 349 184
MscI TGG'CCA
Cuts at: 242 3788 5371 242
Size: 3546 1583 2481
Fragments arranged by size:
3546 2481 1583
MslI CAynn'nnrTG
Cuts at: 2960 3827 5599 5726 2960
Size: 867 1772 127 4844
Fragments arranged by size:
4844 1772 867 127
MspA1I CmG'CkG
Cuts at: 2479 2983 3064 3145 3262 3799 4721 5035 5395
Size: 504 81 81 117 537 922 314 360
Cuts at: 5395 6408 6653 2479
Size: 1013 245 3436
Fragments arranged by size:
3436 1013 922 537 504 360 314 245
117 81 81
NaeI GCC'GGC
Cuts at: 5791 5791
Size: 7610
NarI GG'CG_CC
Cuts at: 5041 5289 5041
Size: 248 7362
NcoI C'CATG_G
Cuts at: 1100 1100
Size: 7610
NgoMIV G'CCGG_C
Cuts at: 5789 5789
Size: 7610
NspI r_CATG'y
Cuts at: 3099 3126 3840 4115 4663 5163 6070 3099
Size: 27 714 275 548 500 907 4639
Fragments arranged by size:
4639 907 714 548 500 275 27
PasI CC'CwG_GG
Cuts at: 373 1984 2658 3325 3676 3904 373
Size: 1611 674 667 351 228 4079
Fragments arranged by size:
4079 1611 674 667 351 228
PciI A'CATG_T
Cuts at: 4111 6066 4111
Size: 1955 5655
PfoI T'CCnGG_A
Cuts at: 498 516 624 633 840 957 1164 1245 1254
Size: 18 108 9 207 117 207 81 9
Cuts at: 1254 1452 1461 1515 1785 2037 2163 2199 2208
Size: 198 9 54 270 252 126 36 9
Cuts at: 2208 2730 2784 2883 3387 498
Size: 522 54 99 504 4721
Fragments arranged by size:
4721 522 504 270 252 207 207 198
126 117 108 99 81 54 54 36
18 9 9 9 9
PmeI GTTT'AAAC
Cuts at: 4609 4609
Size: 7610
PmlI CAC'GTG
Cuts at: 868 3992 868
Size: 3124 4486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6749 6781 6749
Size: 32 7578
PpuMI rG'GwC_Cy
Cuts at: 511 628 655 754 808 817 1105 1213 1276
Size: 117 27 99 54 9 288 108 63
Cuts at: 1276 1303 1465 1618 1744 1762 1852 1906 2113
Size: 27 162 153 126 18 90 54 207
Cuts at: 2113 2230 2357 2509 2536 2662 2887 2896 2923
Size: 117 127 152 27 126 225 9 27
Cuts at: 2923 2943 3015 3427 3481 3634 3733 3778 511
Size: 20 72 412 54 153 99 45 4343
Fragments arranged by size:
4343 412 288 225 207 162 153 153
152 127 126 126 117 117 108 99
99 90 72 63 54 54 54 45
27 27 27 27 20 18 9 9
PshAI GACnn'nnGTC
Cuts at: 642 3524 642
Size: 2882 4728
PspOMI G'GGCC_C
Cuts at: 879 1330 2076 2517 3211 3741 879
Size: 451 746 441 694 530 4748
Fragments arranged by size:
4748 746 694 530 451 441
PspXI vC'TCGA_Gb
Cuts at: 2567 2567
Size: 7610
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1368 1400 4297 4329 1368
Size: 32 2897 32 4649
Fragments arranged by size:
4649 2897 32 32
PstI C_TGCA'G
Cuts at: 3336 4657 5342 3336
Size: 1321 685 5604
Fragments arranged by size:
5604 1321 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7610
PvuII CAG'CTG
Cuts at: 2479 2983 3064 3262 3799 5035 5395 2479
Size: 504 81 198 537 1236 360 4694
Fragments arranged by size:
4694 1236 537 504 360 198 81
RsrII CG'GwC_CG
Cuts at: 5805 5805
Size: 7610
SacI G_AGCT'C
Cuts at: 966 2037 2883 4624 966
Size: 1071 846 1741 3952
Fragments arranged by size:
3952 1741 1071 846
SalI G'TCGA_C
Cuts at: 4647 4647
Size: 7610
SanDI GG'GwC_CC
Cuts at: 2357 2896 2943 3015 3778 2357
Size: 539 47 72 763 6189
Fragments arranged by size:
6189 763 539 72 47
SapI GCTCTTCn'nnn_
Cuts at: 5633 5843 5633
Size: 210 7400
SbfI CC_TGCA'GG
Cuts at: 3336 4657 3336
Size: 1321 6289
ScaI AGT'ACT
Cuts at: 7127 7127
Size: 7610
SexAI A'CCwGG_T
Cuts at: 597 615 831 1056 1227 2181 2379 2559 3036
Size: 18 216 225 171 954 198 180 477
Cuts at: 3036 3441 4150 597
Size: 405 709 4057
Fragments arranged by size:
4057 954 709 477 405 225 216 198
180 171 18
SfcI C'TryA_G
Cuts at: 33 812 1280 1748 2828 3233 3332 3431 4653
Size: 779 468 468 1080 405 99 99 1222
Cuts at: 4653 5338 6331 6522 33
Size: 685 993 191 1121
Fragments arranged by size:
1222 1121 1080 993 779 685 468 468
405 191 99 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 1958 3137 3687 3767 1958
Size: 1179 550 80 5801
Fragments arranged by size:
5801 1179 550 80
SfoI GGC'GCC
Cuts at: 5042 5290 5042
Size: 248 7362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7610
SmaI CCC'GGG
Cuts at: 1941 2596 2913 4632 1941
Size: 655 317 1719 4919
Fragments arranged by size:
4919 1719 655 317
SmlI C'TyrA_G
Cuts at: 666 1730 2567 2612 4765 5680 6172 6434 666
Size: 1064 837 45 2153 915 492 262 1842
Fragments arranged by size:
2153 1842 1064 915 837 492 262 45
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7610
SphI G_CATG'C
Cuts at: 3099 3126 3840 4663 5163 3099
Size: 27 714 823 500 5546
Fragments arranged by size:
5546 823 714 500 27
SspI AAT'ATT
Cuts at: 4422 4422
Size: 7610
StyI C'CwwG_G
Cuts at: 766 1064 1100 2432 2811 2901 3477 4056 4744
Size: 298 36 1332 379 90 576 579 688
Cuts at: 4744 5098 766
Size: 354 3278
Fragments arranged by size:
3278 1332 688 579 576 379 354 298
90 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 665 1084 1867 3370 3527 3995 5193 5326 5785
Size: 419 783 1503 157 468 1198 133 459
Cuts at: 5785 5994 665
Size: 209 2281
Fragments arranged by size:
2281 1503 1198 783 468 459 419 209
157 133
TatI w'GTAC_w
Cuts at: 248 7125 248
Size: 6877 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7127 7159 7127
Size: 32 7578
Tth111I GACn'n_nGTC
Cuts at: 2318 5407 2318
Size: 3089 4521
XbaI T'CTAG_A
Cuts at: 4641 4641
Size: 7610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1089 1107 2818 3484 4148 5105 1089
Size: 18 1711 666 664 957 3594
Fragments arranged by size:
3594 1711 957 666 664 18
XhoI C'TCGA_G
Cuts at: 2567 2612 2567
Size: 45 7565
XmaI C'CCGG_G
Cuts at: 1939 2594 2911 4630 1939
Size: 655 317 1719 4919
Fragments arranged by size:
4919 1719 655 317
ZraI GAC'GTC
Cuts at: 1792 1792
Size: 7610
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AscI BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PflMI PsiI SacII
SgrAI SnaBI SrfI StuI SwaI XmnI