Restriction map for
pF1KB3619
(Circular) (Six-base) MAPSORT of: pF1KB3619.seq Check: 7675 from: 1 to: 5819
pF1KB3619 5819 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 2857 2857
Size: 5819
Acc65I G'GTAC_C
Cuts at: 506 1736 2835 506
Size: 1230 1099 3490
Fragments arranged by size:
3490 1230 1099
AclI AA'CG_TT
Cuts at: 5659 5659
Size: 5819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 1426 2290 2495 3644 4076 4429 5091 279
Size: 1147 864 205 1149 432 353 662 1007
Fragments arranged by size:
1149 1147 1007 864 662 432 353 205
AflIII A'CryG_T
Cuts at: 1245 3171 4971 5252 1245
Size: 1926 1800 281 1812
Fragments arranged by size:
1926 1812 1800 281
AgeI A'CCGG_T
Cuts at: 1562 1562
Size: 5819
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1510 1542 2359 2391 1510
Size: 32 817 32 4938
Fragments arranged by size:
4938 817 32 32
AlwNI CAG_nnn'CTG
Cuts at: 353 3124 4562 353
Size: 2771 1438 1610
Fragments arranged by size:
2771 1610 1438
ApaLI G'TGCA_C
Cuts at: 4657 4657
Size: 5819
ApoI r'AATT_y
Cuts at: 359 585 603 1456 1610 2653 2823 5172 5428
Size: 226 18 853 154 1043 170 2349 256
Cuts at: 5428 359
Size: 750
Fragments arranged by size:
2349 1043 853 750 256 226 170 154
18
AseI AT'TA_AT
Cuts at: 20 1427 20
Size: 1407 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
AvaI C'yCGr_G
Cuts at: 2052 2839 2052
Size: 787 5032
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1104 1137 1720 1753 1104
Size: 33 583 33 5170
Fragments arranged by size:
5170 583 33 33
BamHI G'GATC_C
Cuts at: 2844 2844
Size: 5819
BanI G'GyrC_C
Cuts at: 506 1736 2287 2835 3249 3497 3532 506
Size: 1230 551 548 414 248 35 2793
Fragments arranged by size:
2793 1230 551 548 414 248 35
BanII G_rGCy'C
Cuts at: 135 191 2405 2833 3863 135
Size: 56 2214 428 1030 2091
Fragments arranged by size:
2214 2091 1030 428 56
BbeI G_GCGC'C
Cuts at: 3253 3501 3253
Size: 248 5571
BbsI GAAGACnn'nnnn_
Cuts at: 572 2237 2701 2816 572
Size: 1665 464 115 3575
Fragments arranged by size:
3575 1665 464 115
BciVI GTATCCnnnnn_n'
Cuts at: 2421 3717 4773 5819 2421
Size: 1296 1056 1046 2421
Fragments arranged by size:
2421 1296 1056 1046
BclI T'GATC_A
Cuts at: 3339 3339
Size: 5819
BglII A'GATC_T
Cuts at: 2321 3334 5371 2321
Size: 1013 2037 2769
Fragments arranged by size:
2769 2037 1013
BlpI GC'TnA_GC
Cuts at: 2931 2931
Size: 5819
Bme1580I G_kGCm'C
Cuts at: 3444 3537 4661 3444
Size: 93 1124 4602
Fragments arranged by size:
4602 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 632 3031 3112 3447 5591 632
Size: 2399 81 335 2144 860
Fragments arranged by size:
2399 2144 860 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4186 4186
Size: 5819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 2389 854
Size: 1535 4284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 972 2995 3874 4588 4886 972
Size: 2023 879 714 298 1905
Fragments arranged by size:
2023 1905 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 778 2292 5523 778
Size: 1514 3231 1074
Fragments arranged by size:
3231 1514 1074
BsaAI yAC'GTr
Cuts at: 679 1248 3172 679
Size: 569 1924 3326
Fragments arranged by size:
3326 1924 569
BsaBI GATnn'nnATC
Cuts at: 6 908 3333 5370 6
Size: 902 2425 2037 455
Fragments arranged by size:
2425 2037 902 455
BsaHI Gr'CG_yC
Cuts at: 3250 3498 4200 5696 3250
Size: 248 702 1496 3373
Fragments arranged by size:
3373 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1562 1739 3008 3529 4207 4618 4765 5039 1562
Size: 177 1269 521 678 411 147 274 2342
Fragments arranged by size:
2342 1269 678 521 411 274 177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1813 1843 2359 2389 1813
Size: 30 516 30 5243
Fragments arranged by size:
5243 516 30 30
BseYI C'CCAG_C
Cuts at: 134 2588 3244 4002 4667 5075 134
Size: 2454 656 758 665 408 878
Fragments arranged by size:
2454 878 758 665 656 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1226 1226
Size: 5819
BsiEI CG_ry'CG
Cuts at: 104 128 3361 3407 4637 104
Size: 24 3233 46 1230 1286
Fragments arranged by size:
3233 1286 1230 46 24
BsiHKAI G_wGCw'C
Cuts at: 958 2405 2833 3611 3801 4661 958
Size: 1447 428 778 190 860 2116
Fragments arranged by size:
2116 1447 860 778 428 190
BsmI GAATG_Cn'
Cuts at: 1026 2396 2435 2632 1026
Size: 1370 39 197 4213
Fragments arranged by size:
4213 1370 197 39
Bsp1286I G_dGCh'C
Cuts at: 135 191 958 2405 2833 3444 3537 3611 3801
Size: 56 767 1447 428 611 93 74 190
Cuts at: 3801 3863 4661 135
Size: 62 798 1293
Fragments arranged by size:
1447 1293 798 767 611 428 190 93
74 62 56
BspHI T'CATG_A
Cuts at: 1343 4251 1343
Size: 2908 2911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2869 3083 3385 3766 2869
Size: 214 302 381 4922
Fragments arranged by size:
4922 381 302 214
BsrBI CCG'CTC
Cuts at: 50 120 4111 4165 5649 5814 50
Size: 70 3991 54 1484 165 55
Fragments arranged by size:
3991 1484 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 3731 3731
Size: 5819
BsrFI r'CCGG_y
Cuts at: 1562 3817 3998 1562
Size: 2255 181 3383
Fragments arranged by size:
3383 2255 181
BssSI C'ACGA_G
Cuts at: 4090 4798 4090
Size: 708 5111
BstAPI GCAn_nnn'nTGC
Cuts at: 3196 3196
Size: 5819
BstBI TT'CG_AA
Cuts at: 4180 4180
Size: 5819
BstXI CCAn_nnnn'nTGG
Cuts at: 2386 2736 2386
Size: 350 5469
BstYI r'GATC_y
Cuts at: 1 2321 2567 2844 3311 3334 3669 3915 4221
Size: 2320 246 277 467 23 335 246 306
Cuts at: 4221 4233 4319 5371 1
Size: 12 86 1052 449
Fragments arranged by size:
2320 1052 467 449 335 306 277 246
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2716 2716
Size: 5819
BtgI C'CryG_G
Cuts at: 107 1004 1948 107
Size: 897 944 3978
Fragments arranged by size:
3978 944 897
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2553 3769 3950 4113 2553
Size: 1216 181 163 4259
Fragments arranged by size:
4259 1216 181 163
BtsI GCAGTG_nn'
Cuts at: 2479 3062 2479
Size: 583 5236
DraI TTT'AAA
Cuts at: 2818 2818
Size: 5819
DraIII CAC_nnn'GTG
Cuts at: 2469 2469
Size: 5819
DrdI GACnn_nn'nnGTC
Cuts at: 3525 4869 3525
Size: 1344 4475
EaeI y'GGCC_r
Cuts at: 861 3404 3578 3969 3996 5341 861
Size: 2543 174 391 27 1345 1339
Fragments arranged by size:
2543 1345 1339 391 174 27
EagI C'GGCC_G
Cuts at: 3404 3404
Size: 5819
EarI CTCTTCn'nnn_
Cuts at: 1705 2393 2704 3842 4052 1705
Size: 688 311 1138 210 3472
Fragments arranged by size:
3472 1138 688 311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4027 4769 4915 5629 4027
Size: 742 146 714 4217
Fragments arranged by size:
4217 742 714 146
EcoICRI GAG'CTC
Cuts at: 2403 2831 2403
Size: 428 5391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 854 1426 2290 2389 2495 3644 4076 4429
Size: 575 572 864 99 106 1149 432 353
Cuts at: 4429 5091 279
Size: 662 1007
Fragments arranged by size:
1149 1007 864 662 575 572 432 353
106 99
EcoNI CCTnn'n_nnAGG
Cuts at: 2245 2245
Size: 5819
EcoO109I rG'GnC_Cy
Cuts at: 175 2370 2958 175
Size: 2195 588 3036
Fragments arranged by size:
3036 2195 588
EcoRI G'AATT_C
Cuts at: 1610 2823 1610
Size: 1213 4606
EcoRV GAT'ATC
Cuts at: 273 921 273
Size: 648 5171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 340 3838 340
Size: 3498 2321
FspI TGC'GCA
Cuts at: 3600 3600
Size: 5819
HaeII r_GCGC'y
Cuts at: 393 1697 3253 3501 4731 5502 393
Size: 1304 1556 248 1230 771 710
Fragments arranged by size:
1556 1304 1230 771 710 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 159 191 1254 1286 2567 2599 2679 2711 3661
Size: 32 1063 32 1281 32 80 32 950
Cuts at: 3661 3691 3693 3723 159
Size: 30 2 30 2255
Fragments arranged by size:
2255 1281 1063 950 80 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 816 1503 2858 816
Size: 687 1355 3777
Fragments arranged by size:
3777 1355 687
HindIII A'AGCT_T
Cuts at: 689 1493 689
Size: 804 5015
KasI G'GCGC_C
Cuts at: 3249 3497 3249
Size: 248 5571
KpnI G_GTAC'C
Cuts at: 510 1740 2839 510
Size: 1230 1099 3490
Fragments arranged by size:
3490 1230 1099
MluI A'CGCG_T
Cuts at: 5252 5252
Size: 5819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 108 822 1122 4578 4762 108
Size: 714 300 3456 184 1165
Fragments arranged by size:
3456 1165 714 300 184
MscI TGG'CCA
Cuts at: 863 3580 863
Size: 2717 3102
MslI CAynn'nnrTG
Cuts at: 2609 3808 3935 2609
Size: 1199 127 4493
Fragments arranged by size:
4493 1199 127
MspA1I CmG'CkG
Cuts at: 439 765 2930 3244 3604 4388 4633 439
Size: 326 2165 314 360 784 245 1625
Fragments arranged by size:
2165 1625 784 360 326 314 245
NaeI GCC'GGC
Cuts at: 4000 4000
Size: 5819
NarI GG'CG_CC
Cuts at: 3250 3498 3250
Size: 248 5571
NcoI C'CATG_G
Cuts at: 107 1004 1948 107
Size: 897 944 3978
Fragments arranged by size:
3978 944 897
NgoMIV G'CCGG_C
Cuts at: 3998 3998
Size: 5819
NspI r_CATG'y
Cuts at: 265 1398 2872 3372 4975 265
Size: 1133 1474 500 1603 1109
Fragments arranged by size:
1603 1474 1133 1109 500
PciI A'CATG_T
Cuts at: 4971 4971
Size: 5819
PflMI CCAn_nnn'nTGG
Cuts at: 945 2469 945
Size: 1524 4295
PfoI T'CCnGG_A
Cuts at: 182 182
Size: 5819
PmeI GTTT'AAAC
Cuts at: 2818 2818
Size: 5819
PmlI CAC'GTG
Cuts at: 679 1248 679
Size: 569 5250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2391 4265 4297 2359
Size: 32 1874 32 3881
Fragments arranged by size:
3881 1874 32 32
PpuMI rG'GwC_Cy
Cuts at: 175 2370 175
Size: 2195 3624
PsiI TTA'TAA
Cuts at: 1280 1541 1280
Size: 261 5558
PstI C_TGCA'G
Cuts at: 2866 3551 2866
Size: 685 5134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5819
PvuII CAG'CTG
Cuts at: 439 3244 3604 439
Size: 2805 360 2654
Fragments arranged by size:
2805 2654 360
RsrII CG'GwC_CG
Cuts at: 4014 4014
Size: 5819
SacI G_AGCT'C
Cuts at: 2405 2833 2405
Size: 428 5391
SalI G'TCGA_C
Cuts at: 2856 2856
Size: 5819
SapI GCTCTTCn'nnn_
Cuts at: 2393 3842 4052 2393
Size: 1449 210 4160
Fragments arranged by size:
4160 1449 210
SbfI CC_TGCA'GG
Cuts at: 2866 2866
Size: 5819
ScaI AGT'ACT
Cuts at: 1939 5336 1939
Size: 3397 2422
SfcI C'TryA_G
Cuts at: 33 1010 1130 2862 3547 4515 4706 33
Size: 977 120 1732 685 968 191 1146
Fragments arranged by size:
1732 1146 977 968 685 191 120
SfoI GGC'GCC
Cuts at: 3251 3499 3251
Size: 248 5571
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
SmaI CCC'GGG
Cuts at: 2841 2841
Size: 5819
SmlI C'TyrA_G
Cuts at: 951 2974 3889 4603 4865 951
Size: 2023 915 714 262 1905
Fragments arranged by size:
2023 1905 915 714 262
SpeI A'CTAG_T
Cuts at: 67 2704 67
Size: 2637 3182
SphI G_CATG'C
Cuts at: 1398 2872 3372 1398
Size: 1474 500 3845
Fragments arranged by size:
3845 1474 500
SspI AAT'ATT
Cuts at: 1473 1473
Size: 5819
StuI AGG'CCT
Cuts at: 2721 2721
Size: 5819
StyI C'CwwG_G
Cuts at: 107 889 1004 1948 2315 2953 3307 107
Size: 782 115 944 367 638 354 2619
Fragments arranged by size:
2619 944 782 638 367 354 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1622 2742 3402 3535 3994 4203 1622
Size: 1120 660 133 459 209 3238
Fragments arranged by size:
3238 1120 660 459 209 133
TatI w'GTAC_w
Cuts at: 1937 5334 1937
Size: 3397 2422
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5336 5368 5336
Size: 32 5787
Tth111I GACn'n_nGTC
Cuts at: 3616 3616
Size: 5819
XbaI T'CTAG_A
Cuts at: 2850 2850
Size: 5819
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3314 3314
Size: 5819
XmaI C'CCGG_G
Cuts at: 2839 2839
Size: 5819
XmnI GAAnn'nnTTC
Cuts at: 2075 2075
Size: 5819
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI