Restriction map for
pF1KB3626
(Circular) (Six-base) MAPSORT of: pF1KB3626.seq Check: 7935 from: 1 to: 6575
pF1KB3626 6575 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1555 1557 2808 1555
Size: 2 1251 5322
Fragments arranged by size:
5322 1251 2
AatII G_ACGT'C
Cuts at: 3299 3299
Size: 6575
AccI GT'mk_AC
Cuts at: 1808 3613 1808
Size: 1805 4770
Acc65I G'GTAC_C
Cuts at: 3591 3591
Size: 6575
AclI AA'CG_TT
Cuts at: 1034 6415 1034
Size: 5381 1194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1150 1309 1964 2717 2857 4400 4832 5579
Size: 225 159 655 753 140 1543 432 747
Cuts at: 5579 5847 925
Size: 268 1653
Fragments arranged by size:
1653 1543 753 747 655 432 268 225
159 140
AfeI AGC'GCT
Cuts at: 2725 2725
Size: 6575
AflII C'TTAA_G
Cuts at: 2892 2998 2892
Size: 106 6469
AflIII A'CryG_T
Cuts at: 3927 5031 6008 3927
Size: 1104 977 4494
Fragments arranged by size:
4494 1104 977
AgeI A'CCGG_T
Cuts at: 4963 4963
Size: 6575
AhdI GACnn_n'nnGTC
Cuts at: 408 3300 408
Size: 2892 3683
AleI CACnn'nnGTG
Cuts at: 1384 1384
Size: 6575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 324 324
Size: 6575
AlwNI CAG_nnn'CTG
Cuts at: 1698 2695 3880 5447 1698
Size: 997 1185 1567 2826
Fragments arranged by size:
2826 1567 1185 997
ApaLI G'TGCA_C
Cuts at: 5345 5345
Size: 6575
ApoI r'AATT_y
Cuts at: 856 2179 2977 3277 3505 3579 5928 6184 856
Size: 1323 798 300 228 74 2349 256 1247
Fragments arranged by size:
2349 1323 1247 798 300 256 228 74
AseI AT'TA_AT
Cuts at: 20 2642 20
Size: 2622 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6575
AvaI C'yCGr_G
Cuts at: 1751 3595 1751
Size: 1844 4731
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 559 592 559
Size: 33 6542
BamHI G'GATC_C
Cuts at: 1838 2738 3600 1838
Size: 900 862 4813
Fragments arranged by size:
4813 900 862
BanI G'GyrC_C
Cuts at: 1576 3591 4005 4253 4288 1576
Size: 2015 414 248 35 3863
Fragments arranged by size:
3863 2015 414 248 35
BanII G_rGCy'C
Cuts at: 172 248 2780 3589 4619 172
Size: 76 2532 809 1030 2128
Fragments arranged by size:
2532 2128 1030 809 76
BbeI G_GCGC'C
Cuts at: 4009 4257 4009
Size: 248 6327
BbsI GAAGACnn'nnnn_
Cuts at: 184 2951 184
Size: 2767 3808
BbvCI CC'TCA_GC
Cuts at: 1501 1501
Size: 6575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6541
BciVI GTATCCnnnnn_n'
Cuts at: 4473 5234 6575 4473
Size: 761 1341 4473
Fragments arranged by size:
4473 1341 761
BclI T'GATC_A
Cuts at: 4095 4095
Size: 6575
BfrBI ATG'CAT
Cuts at: 2363 2363
Size: 6575
BglI GCCn_nnn'nGGC
Cuts at: 252 252
Size: 6575
BglII A'GATC_T
Cuts at: 2888 4090 6127 2888
Size: 1202 2037 3336
Fragments arranged by size:
3336 2037 1202
BlpI GC'TnA_GC
Cuts at: 3687 3687
Size: 6575
Bme1580I G_kGCm'C
Cuts at: 1581 2211 3457 4200 4293 5349 1581
Size: 630 1246 743 93 1056 2807
Fragments arranged by size:
2807 1246 1056 743 630 93
BmgBI CAC'GTC
Cuts at: 1386 2088 1386
Size: 702 5873
BmrI ACTGGGnnnn_n'
Cuts at: 1070 3787 3868 4203 6347 1070
Size: 2717 81 335 2144 1298
Fragments arranged by size:
2717 2144 1298 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4942 4942
Size: 6575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 592 3239 214
Size: 378 2647 3550
Fragments arranged by size:
3550 2647 378
Bpu10I CC'TnA_GC
Cuts at: 203 587 1501 2212 203
Size: 384 914 711 4566
Fragments arranged by size:
4566 914 711 384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 1828 3751 4630 5122 5420 1669
Size: 159 1923 879 492 298 2824
Fragments arranged by size:
2824 1923 879 492 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 623 1661 2046 6279 623
Size: 1038 385 4233 919
Fragments arranged by size:
4233 1038 919 385
BsaAI yAC'GTr
Cuts at: 1497 3928 1497
Size: 2431 4144
BsaBI GATnn'nnATC
Cuts at: 6 1364 4089 6126 6
Size: 1358 2725 2037 455
Fragments arranged by size:
2725 2037 1358 455
BsaHI Gr'CG_yC
Cuts at: 489 2072 3296 4006 4254 4956 6452 489
Size: 1583 1224 710 248 702 1496 612
Fragments arranged by size:
1583 1496 1224 710 702 612 248
BsaWI w'CCGG_w
Cuts at: 428 2147 3764 4285 4963 5237 5384 5795 428
Size: 1719 1617 521 678 274 147 411 1208
Fragments arranged by size:
1719 1617 1208 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 1300 1330 1477 1507 565
Size: 30 705 30 147 30 5633
Fragments arranged by size:
5633 705 147 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 180 311 1183 1321 1498 2257 180
Size: 131 872 138 177 759 4498
Fragments arranged by size:
4498 872 759 177 138 131
BseYI C'CCAG_C
Cuts at: 255 1657 1984 3500 4000 4758 5335 5831 255
Size: 1402 327 1516 500 758 577 496 999
Fragments arranged by size:
1516 1402 999 758 577 500 496 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 1362 1497 1591 1618 1633 1879 2556 2922
Size: 1078 135 94 27 15 246 677 366
Cuts at: 2922 284
Size: 3937
Fragments arranged by size:
3937 1078 677 366 246 135 94 27
15
BsiEI CG_ry'CG
Cuts at: 104 1836 4117 4163 5371 104
Size: 1732 2281 46 1208 1308
Fragments arranged by size:
2281 1732 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 172 1871 2780 3589 4367 4557 5349 172
Size: 1699 909 809 778 190 792 1398
Fragments arranged by size:
1699 1398 909 809 792 778 190
BsmI GAATG_Cn'
Cuts at: 2365 2365
Size: 6575
Bsp1286I G_dGCh'C
Cuts at: 172 248 1581 1871 2211 2780 3457 3589 4200
Size: 76 1333 290 340 569 677 132 611
Cuts at: 4200 4293 4367 4557 4619 5349 172
Size: 93 74 190 62 730 1398
Fragments arranged by size:
1398 1333 730 677 611 569 340 290
190 132 93 76 74 62
BspEI T'CCGG_A
Cuts at: 5795 5795
Size: 6575
BspHI T'CATG_A
Cuts at: 1535 1673 2576 2630 2907 5751 1535
Size: 138 903 54 277 2844 2359
Fragments arranged by size:
2844 2359 903 277 138 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1555 1557 1775 2774 2808 3625 3839 4141 4522
Size: 2 218 999 34 817 214 302 381
Cuts at: 4522 1555
Size: 3608
Fragments arranged by size:
3608 999 817 381 302 218 214 34
2
BsrBI CCG'CTC
Cuts at: 50 4867 4921 6405 6570 50
Size: 4817 54 1484 165 55
Fragments arranged by size:
4817 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 144 4487 144
Size: 4343 2232
BsrFI r'CCGG_y
Cuts at: 2531 4573 4754 4963 2531
Size: 2042 181 209 4143
Fragments arranged by size:
4143 2042 209 181
BsrGI T'GTAC_A
Cuts at: 1441 1441
Size: 6575
BssSI C'ACGA_G
Cuts at: 1303 1884 4846 5204 1303
Size: 581 2962 358 2674
Fragments arranged by size:
2962 2674 581 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1698 3327 3952 1698
Size: 1629 625 4321
Fragments arranged by size:
4321 1629 625
BstBI TT'CG_AA
Cuts at: 1018 4936 1018
Size: 3918 2657
BstEII G'GTnAC_C
Cuts at: 2854 2854
Size: 6575
BstXI CCAn_nnnn'nTGG
Cuts at: 570 1770 2199 570
Size: 1200 429 4946
Fragments arranged by size:
4946 1200 429
BstYI r'GATC_y
Cuts at: 1 1181 1694 1838 1851 2133 2480 2738 2888
Size: 1180 513 144 13 282 347 258 150
Cuts at: 2888 3600 4067 4090 4425 4671 5683 5769 5781
Size: 712 467 23 335 246 1012 86 12
Cuts at: 5781 6127 1
Size: 346 449
Fragments arranged by size:
1180 1012 712 513 467 449 347 346
335 282 258 246 150 144 86 23
13 12
BtgI C'CryG_G
Cuts at: 143 2173 2241 2377 3359 3393 143
Size: 2030 68 136 982 34 3325
Fragments arranged by size:
3325 2030 982 136 68 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2171 4525 4706 4869 2171
Size: 2354 181 163 3877
Fragments arranged by size:
3877 2354 181 163
BtsI GCAGTG_nn'
Cuts at: 267 3818 267
Size: 3551 3024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2681 2697 2716 2732 2681
Size: 16 19 16 6524
Fragments arranged by size:
6524 19 16 16
DraI TTT'AAA
Cuts at: 3566 3574 3566
Size: 8 6567
DraIII CAC_nnn'GTG
Cuts at: 2058 3156 2058
Size: 1098 5477
DrdI GACnn_nn'nnGTC
Cuts at: 4281 5139 4281
Size: 858 5717
EaeI y'GGCC_r
Cuts at: 692 2201 3396 4160 4334 4725 4752 6097 692
Size: 1509 1195 764 174 391 27 1345 1170
Fragments arranged by size:
1509 1345 1195 1170 764 391 174 27
EagI C'GGCC_G
Cuts at: 4160 4160
Size: 6575
EarI CTCTTCn'nnn_
Cuts at: 1184 1302 1940 2102 2143 2648 4598 4808 1184
Size: 118 638 162 41 505 1950 210 2951
Fragments arranged by size:
2951 1950 638 505 210 162 118 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4783 5093 5239 6385 4783
Size: 310 146 1146 4973
Fragments arranged by size:
4973 1146 310 146
EcoICRI GAG'CTC
Cuts at: 170 2778 3587 170
Size: 2608 809 3158
Fragments arranged by size:
3158 2608 809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 592 925 1150 1309 1964 2717 2857 3239
Size: 378 333 225 159 655 753 140 382
Cuts at: 3239 4400 4832 5579 5847 214
Size: 1161 432 747 268 942
Fragments arranged by size:
1161 942 753 747 655 432 382 378
333 268 225 159 140
EcoNI CCTnn'n_nnAGG
Cuts at: 206 1846 206
Size: 1640 4935
EcoO109I rG'GnC_Cy
Cuts at: 198 1907 2125 3714 198
Size: 1709 218 1589 3059
Fragments arranged by size:
3059 1709 1589 218
EcoRI G'AATT_C
Cuts at: 3505 3579 3505
Size: 74 6501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2457 4594 2457
Size: 2137 4438
FspI TGC'GCA
Cuts at: 1702 4356 1702
Size: 2654 3921
HaeII r_GCGC'y
Cuts at: 2727 4009 4257 5279 6258 2727
Size: 1282 248 1022 979 3044
Fragments arranged by size:
3044 1282 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 394 426 550 582 1050 1082 1300
Size: 32 149 32 124 32 468 32 218
Cuts at: 1300 1332 1818 1850 3286 3318 4417 4447 4449
Size: 32 486 32 1436 32 1099 30 2
Cuts at: 4449 4479 213
Size: 30 2309
Fragments arranged by size:
2309 1436 1099 486 468 218 149 124
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3614 3614
Size: 6575
HindIII A'AGCT_T
Cuts at: 2647 3528 2647
Size: 881 5694
KasI G'GCGC_C
Cuts at: 4005 4253 4005
Size: 248 6327
KpnI G_GTAC'C
Cuts at: 3595 3595
Size: 6575
MfeI C'AATT_G
Cuts at: 450 3188 450
Size: 2738 3837
MluI A'CGCG_T
Cuts at: 6008 6008
Size: 6575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 215 1787 3534 5246 5430 215
Size: 1572 1747 1712 184 1360
Fragments arranged by size:
1747 1712 1572 1360 184
MscI TGG'CCA
Cuts at: 694 2203 3398 4336 694
Size: 1509 1195 938 2933
Fragments arranged by size:
2933 1509 1195 938
MslI CAynn'nnrTG
Cuts at: 306 817 950 1019 1384 1534 2246 2341 2553
Size: 511 133 69 365 150 712 95 212
Cuts at: 2553 4564 4691 306
Size: 2011 127 2190
Fragments arranged by size:
2190 2011 712 511 365 212 150 133
127 95 69
MspA1I CmG'CkG
Cuts at: 1824 2199 2585 3686 4000 4360 5373 5618 1824
Size: 375 386 1101 314 360 1013 245 2781
Fragments arranged by size:
2781 1101 1013 386 375 360 314 245
NaeI GCC'GGC
Cuts at: 4756 4756
Size: 6575
NarI GG'CG_CC
Cuts at: 4006 4254 4006
Size: 248 6327
NcoI C'CATG_G
Cuts at: 143 2173 2241 2377 3359 3393 143
Size: 2030 68 136 982 34 3325
Fragments arranged by size:
3325 2030 982 136 68 34
NgoMIV G'CCGG_C
Cuts at: 4754 4754
Size: 6575
NsiI A_TGCA'T
Cuts at: 2365 2365
Size: 6575
NspI r_CATG'y
Cuts at: 311 3628 4128 5035 311
Size: 3317 500 907 1851
Fragments arranged by size:
3317 1851 907 500
PciI A'CATG_T
Cuts at: 5031 5031
Size: 6575
PflMI CCAn_nnn'nTGG
Cuts at: 368 2174 2456 3394 368
Size: 1806 282 938 3549
Fragments arranged by size:
3549 1806 938 282
PfoI T'CCnGG_A
Cuts at: 1184 1184
Size: 6575
PmeI GTTT'AAAC
Cuts at: 3574 3574
Size: 6575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1277 1309 5714 5746 1277
Size: 32 4405 32 2106
Fragments arranged by size:
4405 2106 32 32
PpuMI rG'GwC_Cy
Cuts at: 1907 2125 1907
Size: 218 6357
PstI C_TGCA'G
Cuts at: 2785 3622 4307 2785
Size: 837 685 5053
Fragments arranged by size:
5053 837 685
PvuI CG_AT'CG
Cuts at: 104 1836 104
Size: 1732 4843
PvuII CAG'CTG
Cuts at: 1824 2199 4000 4360 1824
Size: 375 1801 360 4039
Fragments arranged by size:
4039 1801 375 360
RsrII CG'GwC_CG
Cuts at: 1644 4770 1644
Size: 3126 3449
SacI G_AGCT'C
Cuts at: 172 2780 3589 172
Size: 2608 809 3158
Fragments arranged by size:
3158 2608 809
SalI G'TCGA_C
Cuts at: 3612 3612
Size: 6575
SanDI GG'GwC_CC
Cuts at: 1907 1907
Size: 6575
SapI GCTCTTCn'nnn_
Cuts at: 2648 4598 4808 2648
Size: 1950 210 4415
Fragments arranged by size:
4415 1950 210
SbfI CC_TGCA'GG
Cuts at: 3622 3622
Size: 6575
ScaI AGT'ACT
Cuts at: 6092 6092
Size: 6575
SexAI A'CCwGG_T
Cuts at: 2234 2234
Size: 6575
SfcI C'TryA_G
Cuts at: 33 1626 2781 3618 4303 5296 5487 33
Size: 1593 1155 837 685 993 191 1121
Fragments arranged by size:
1593 1155 1121 993 837 685 191
SfoI GGC'GCC
Cuts at: 4007 4255 4007
Size: 248 6327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6575
SmaI CCC'GGG
Cuts at: 3597 3597
Size: 6575
SmlI C'TyrA_G
Cuts at: 1684 1843 2892 2998 3730 4645 5137 5399 1684
Size: 159 1049 106 732 915 492 262 2860
Fragments arranged by size:
2860 1049 915 732 492 262 159 106
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6575
SphI G_CATG'C
Cuts at: 311 3628 4128 311
Size: 3317 500 2758
Fragments arranged by size:
3317 2758 500
SspI AAT'ATT
Cuts at: 962 962
Size: 6575
StuI AGG'CCT
Cuts at: 1312 2613 1312
Size: 1301 5274
StyI C'CwwG_G
Cuts at: 143 743 926 1872 2173 2241 2377 3359 3393
Size: 600 183 946 301 68 136 982 34
Cuts at: 3393 3709 4063 143
Size: 316 354 2655
Fragments arranged by size:
2655 982 946 600 354 316 301 183
136 68 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 655 4158 4291 4750 4959 655
Size: 3503 133 459 209 2271
Fragments arranged by size:
3503 2271 459 209 133
TatI w'GTAC_w
Cuts at: 269 1441 2254 2924 6090 269
Size: 1172 813 670 3166 754
Fragments arranged by size:
3166 1172 813 754 670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6092 6124 6092
Size: 32 6543
Tth111I GACn'n_nGTC
Cuts at: 2236 4372 2236
Size: 2136 4439
XbaI T'CTAG_A
Cuts at: 3606 3606
Size: 6575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1770 3160 4070 1770
Size: 1390 910 4275
Fragments arranged by size:
4275 1390 910
XmaI C'CCGG_G
Cuts at: 3595 3595
Size: 6575
XmnI GAAnn'nnTTC
Cuts at: 1588 3008 1588
Size: 1420 5155
ZraI GAC'GTC
Cuts at: 3297 3297
Size: 6575
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BmtI BsiWI BsmBI BssHII
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SwaI XhoI