Restriction map for
pF1KB3630
(Circular) (Six-base) MAPSORT of: pF1KB3630.seq Check: 5046 from: 1 to: 3692
pF1KB3630 3692 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 252 252
Size: 3692
AccI GT'mk_AC
Cuts at: 206 730 206
Size: 524 3168
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1517 1949 2696 2964 165
Size: 1352 432 747 268 893
Fragments arranged by size:
1352 893 747 432 268
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 997 2564 997
Size: 1567 2125
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 696 3045 3301 696
Size: 2349 256 1087
Fragments arranged by size:
2349 1087 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 632 712 632
Size: 80 3612
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 148 708 1122 1370 1405 148
Size: 560 414 248 35 2435
Fragments arranged by size:
2435 560 414 248 35
BanII G_rGCy'C
Cuts at: 521 706 1736 521
Size: 185 1030 2477
Fragments arranged by size:
2477 1030 185
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BbsI GAAGACnn'nnnn_
Cuts at: 166 171 305 166
Size: 5 134 3553
Fragments arranged by size:
3553 134 5
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglI GCCn_nnn'nGGC
Cuts at: 634 634
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 585 804 585
Size: 219 3473
Bme1580I G_kGCm'C
Cuts at: 440 1317 1410 2466 440
Size: 877 93 1056 1666
Fragments arranged by size:
1666 1056 877 93
BmrI ACTGGGnnnn_n'
Cuts at: 904 985 1320 3464 904
Size: 81 335 2144 1132
Fragments arranged by size:
2144 1132 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 3692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 628
Size: 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 868 1747 2239 2537 598
Size: 270 879 492 298 1753
Fragments arranged by size:
1753 879 492 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 198 554 881 1402 2080 2354 2501 2912 198
Size: 356 327 521 678 274 147 411 978
Fragments arranged by size:
978 678 521 411 356 327 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 660 690 660
Size: 30 3662
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 160
Size: 3692
BseYI C'CCAG_C
Cuts at: 1117 1875 2452 2948 1117
Size: 758 577 496 1861
Fragments arranged by size:
1861 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 485 586 706 1484 1674 2466 485
Size: 101 120 778 190 792 1711
Fragments arranged by size:
1711 792 778 190 120 101
Bsp1286I G_dGCh'C
Cuts at: 440 485 521 586 706 1317 1410 1484 1674
Size: 45 36 65 120 611 93 74 190
Cuts at: 1674 1736 2466 440
Size: 62 730 1666
Fragments arranged by size:
1666 730 611 190 120 93 74 65
62 45 36
BspEI T'CCGG_A
Cuts at: 198 2912 198
Size: 2714 978
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 172 252 742 956 1258 1639 172
Size: 80 490 214 302 381 2225
Fragments arranged by size:
2225 490 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 1690 1871 2080 1690
Size: 181 209 3302
Fragments arranged by size:
3302 209 181
BssSI C'ACGA_G
Cuts at: 1963 2321 1963
Size: 358 3334
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstXI CCAn_nnnn'nTGG
Cuts at: 447 447
Size: 3692
BstYI r'GATC_y
Cuts at: 1 717 1184 1207 1542 1788 2800 2886 2898
Size: 716 467 23 335 246 1012 86 12
Cuts at: 2898 3244 1
Size: 346 449
Fragments arranged by size:
1012 716 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 469 469
Size: 3692
BtgI C'CryG_G
Cuts at: 107 107
Size: 3692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 935 935
Size: 3692
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 340 1398 2256 340
Size: 1058 858 1776
Fragments arranged by size:
1776 1058 858
EaeI y'GGCC_r
Cuts at: 500 626 1277 1451 1842 1869 3214 500
Size: 126 651 174 391 27 1345 978
Fragments arranged by size:
1345 978 651 391 174 126 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 628 1517 1949 2696 2964 165
Size: 463 889 432 747 268 893
Fragments arranged by size:
893 889 747 463 432 268
EcoO109I rG'GnC_Cy
Cuts at: 465 831 465
Size: 366 3326
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 1126 1374 2396 3375 1126
Size: 248 1022 979 1443
Fragments arranged by size:
1443 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 615 647 660 692 1534 1564 1566 1596 615
Size: 32 13 32 842 30 2 30 2711
Fragments arranged by size:
2711 842 32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 646 2363 2547 646
Size: 1717 184 1791
Fragments arranged by size:
1791 1717 184
MscI TGG'CCA
Cuts at: 502 628 1453 502
Size: 126 825 2741
Fragments arranged by size:
2741 825 126
MslI CAynn'nnrTG
Cuts at: 247 349 448 1681 1808 247
Size: 102 99 1233 127 2131
Fragments arranged by size:
2131 1233 127 102 99
MspA1I CmG'CkG
Cuts at: 803 1117 1477 2490 2735 803
Size: 314 360 1013 245 1760
Fragments arranged by size:
1760 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NcoI C'CATG_G
Cuts at: 107 107
Size: 3692
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NspI r_CATG'y
Cuts at: 745 1245 2152 745
Size: 500 907 2285
Fragments arranged by size:
2285 907 500
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PpuMI rG'GwC_Cy
Cuts at: 465 465
Size: 3692
PstI C_TGCA'G
Cuts at: 739 1424 739
Size: 685 3007
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 1117 1477 1117
Size: 360 3332
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 735 1420 2413 2604 33
Size: 702 685 993 191 1121
Fragments arranged by size:
1121 993 702 685 191
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 714 714
Size: 3692
SmlI C'TyrA_G
Cuts at: 577 847 1762 2254 2516 577
Size: 270 915 492 262 1753
Fragments arranged by size:
1753 915 492 270 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StyI C'CwwG_G
Cuts at: 107 596 826 1180 107
Size: 489 230 354 2619
Fragments arranged by size:
2619 489 354 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 515 1275 1408 1867 2076 515
Size: 760 133 459 209 2131
Fragments arranged by size:
2131 760 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 495 1187 447
Size: 48 692 2952
Fragments arranged by size:
2952 692 48
XmaI C'CCGG_G
Cuts at: 712 712
Size: 3692
XmnI GAAnn'nnTTC
Cuts at: 389 421 389
Size: 32 3660
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI