Restriction map for
pF1KB3632
(Circular) (Six-base) MAPSORT of: pF1KB3632.seq Check: 3636 from: 1 to: 4214
pF1KB3632 4214 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1252 1252
Size: 4214
Acc65I G'GTAC_C
Cuts at: 408 1230 408
Size: 822 3392
AclI AA'CG_TT
Cuts at: 1208 4054 1208
Size: 2846 1368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 2039 2471 3218 3486 169
Size: 1870 432 747 268 897
Fragments arranged by size:
1870 897 747 432 268
AfeI AGC'GCT
Cuts at: 643 643
Size: 4214
AflIII A'CryG_T
Cuts at: 280 549 1152 1566 2670 3647 280
Size: 269 603 414 1104 977 847
Fragments arranged by size:
1104 977 847 603 414 269
AgeI A'CCGG_T
Cuts at: 2602 2602
Size: 4214
AlwNI CAG_nnn'CTG
Cuts at: 324 387 1080 1519 3086 324
Size: 63 693 439 1567 1452
Fragments arranged by size:
1567 1452 693 439 63
ApaLI G'TGCA_C
Cuts at: 2984 2984
Size: 4214
ApoI r'AATT_y
Cuts at: 1218 3567 3823 1218
Size: 2349 256 1609
Fragments arranged by size:
2349 1609 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 254 980 1234 254
Size: 726 254 3234
Fragments arranged by size:
3234 726 254
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 392 425 392
Size: 33 4181
BamHI G'GATC_C
Cuts at: 474 1239 474
Size: 765 3449
BanI G'GyrC_C
Cuts at: 408 574 736 867 1159 1230 1644 1892 1927
Size: 166 162 131 292 71 414 248 35
Cuts at: 1927 408
Size: 2695
Fragments arranged by size:
2695 414 292 248 166 162 131 71
35
BanII G_rGCy'C
Cuts at: 1228 2258 1228
Size: 1030 3184
BbeI G_GCGC'C
Cuts at: 1163 1648 1896 1163
Size: 485 248 3481
Fragments arranged by size:
3481 485 248
BbsI GAAGACnn'nnnn_
Cuts at: 1003 1003
Size: 4214
BciVI GTATCCnnnnn_n'
Cuts at: 2112 2873 4214 2112
Size: 761 1341 2112
Fragments arranged by size:
2112 1341 761
BclI T'GATC_A
Cuts at: 881 1734 881
Size: 853 3361
BfrBI ATG'CAT
Cuts at: 1042 1042
Size: 4214
BglI GCCn_nnn'nGGC
Cuts at: 224 224
Size: 4214
BglII A'GATC_T
Cuts at: 1729 3766 1729
Size: 2037 2177
BlpI GC'TnA_GC
Cuts at: 1326 1326
Size: 4214
Bme1580I G_kGCm'C
Cuts at: 579 741 878 1839 1932 2988 579
Size: 162 137 961 93 1056 1805
Fragments arranged by size:
1805 1056 961 162 137 93
BmgBI CAC'GTC
Cuts at: 550 1192 550
Size: 642 3572
BmrI ACTGGGnnnn_n'
Cuts at: 397 1426 1507 1842 3986 397
Size: 1029 81 335 2144 625
Fragments arranged by size:
2144 1029 625 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 934
Size: 4214
Bpu10I CC'TnA_GC
Cuts at: 632 893 632
Size: 261 3953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 1390 2269 2761 3059 603
Size: 787 879 492 298 1758
Fragments arranged by size:
1758 879 787 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 651 3918 651
Size: 3267 947
BsaAI yAC'GTr
Cuts at: 281 1567 281
Size: 1286 2928
BsaBI GATnn'nnATC
Cuts at: 6 1728 3765 6
Size: 1722 2037 455
Fragments arranged by size:
2037 1722 455
BsaHI Gr'CG_yC
Cuts at: 1160 1645 1893 2595 4091 1160
Size: 485 248 702 1496 1283
Fragments arranged by size:
1496 1283 702 485 248
BsaWI w'CCGG_w
Cuts at: 141 1403 1924 2602 2876 3023 3434 141
Size: 1262 521 678 274 147 411 921
Fragments arranged by size:
1262 921 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 931 1063 1066 931
Size: 132 3 4079
Fragments arranged by size:
4079 132 3
BseYI C'CCAG_C
Cuts at: 424 1639 2397 2974 3470 424
Size: 1215 758 577 496 1168
Fragments arranged by size:
1215 1168 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1756 1802 3010 104
Size: 1652 46 1208 1308
Fragments arranged by size:
1652 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1228 2006 2196 2988 1228
Size: 778 190 792 2454
Fragments arranged by size:
2454 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 579 741 878 1228 1839 1932 2006 2196 2258
Size: 162 137 350 611 93 74 190 62
Cuts at: 2258 2988 579
Size: 730 1805
Fragments arranged by size:
1805 730 611 350 190 162 137 93
74 62
BspEI T'CCGG_A
Cuts at: 141 3434 141
Size: 3293 921
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1264 1478 1780 2161 1264
Size: 214 302 381 3317
Fragments arranged by size:
3317 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2506 2560 4044 4209 50
Size: 2456 54 1484 165 55
Fragments arranged by size:
2456 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 745 1006 1106 2126 745
Size: 261 100 1020 2833
Fragments arranged by size:
2833 1020 261 100
BsrFI r'CCGG_y
Cuts at: 2212 2393 2602 2212
Size: 181 209 3824
Fragments arranged by size:
3824 209 181
BssSI C'ACGA_G
Cuts at: 2485 2843 2485
Size: 358 3856
BstAPI GCAn_nnn'nTGC
Cuts at: 324 1591 324
Size: 1267 2947
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstEII G'GTnAC_C
Cuts at: 399 399
Size: 4214
BstYI r'GATC_y
Cuts at: 1 474 1239 1706 1729 2064 2310 3322 3408
Size: 473 765 467 23 335 246 1012 86
Cuts at: 3408 3420 3766 1
Size: 12 346 449
Fragments arranged by size:
1012 765 473 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 131 293 662 131
Size: 162 369 3683
Fragments arranged by size:
3683 369 162
BtgI C'CryG_G
Cuts at: 107 404 580 626 1122 107
Size: 297 176 46 496 3199
Fragments arranged by size:
3199 496 297 176 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1084 2164 2345 2508 1084
Size: 1080 181 163 2790
Fragments arranged by size:
2790 1080 181 163
BtsI GCAGTG_nn'
Cuts at: 322 805 1457 322
Size: 483 652 3079
Fragments arranged by size:
3079 652 483
DraI TTT'AAA
Cuts at: 1213 1213
Size: 4214
DrdI GACnn_nn'nnGTC
Cuts at: 1920 2778 1920
Size: 858 3356
EaeI y'GGCC_r
Cuts at: 827 1799 1973 2364 2391 3736 827
Size: 972 174 391 27 1345 1305
Fragments arranged by size:
1345 1305 972 391 174 27
EagI C'GGCC_G
Cuts at: 1799 1799
Size: 4214
EarI CTCTTCn'nnn_
Cuts at: 443 796 2237 2447 443
Size: 353 1441 210 2210
Fragments arranged by size:
2210 1441 353 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2422 2732 2878 4024 2422
Size: 310 146 1146 2612
Fragments arranged by size:
2612 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1226 1226
Size: 4214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 934 2039 2471 3218 3486 169
Size: 765 1105 432 747 268 897
Fragments arranged by size:
1105 897 765 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 416 416
Size: 4214
EcoO109I rG'GnC_Cy
Cuts at: 127 421 456 1353 127
Size: 294 35 897 2988
Fragments arranged by size:
2988 897 294 35
EcoRI G'AATT_C
Cuts at: 1218 1218
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 1995 1995
Size: 4214
HaeII r_GCGC'y
Cuts at: 645 1163 1648 1896 2918 3897 645
Size: 518 485 248 1022 979 962
Fragments arranged by size:
1022 979 962 518 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2056 2086 2088 2118 2056
Size: 30 2 30 4152
Fragments arranged by size:
4152 30 30 2
HincII GTy'rAC
Cuts at: 1253 1253
Size: 4214
KasI G'GCGC_C
Cuts at: 1159 1644 1892 1159
Size: 485 248 3481
Fragments arranged by size:
3481 485 248
KpnI G_GTAC'C
Cuts at: 412 1234 412
Size: 822 3392
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 386 2885 3069 386
Size: 2499 184 1531
Fragments arranged by size:
2499 1531 184
MscI TGG'CCA
Cuts at: 829 1975 829
Size: 1146 3068
MslI CAynn'nnrTG
Cuts at: 397 2203 2330 397
Size: 1806 127 2281
Fragments arranged by size:
2281 1806 127
MspA1I CmG'CkG
Cuts at: 558 678 753 1325 1639 1999 3012 3257 558
Size: 120 75 572 314 360 1013 245 1515
Fragments arranged by size:
1515 1013 572 360 314 245 120 75
NaeI GCC'GGC
Cuts at: 2395 2395
Size: 4214
NarI GG'CG_CC
Cuts at: 1160 1645 1893 1160
Size: 485 248 3481
Fragments arranged by size:
3481 485 248
NcoI C'CATG_G
Cuts at: 107 404 580 626 1122 107
Size: 297 176 46 496 3199
Fragments arranged by size:
3199 496 297 176 46
NgoMIV G'CCGG_C
Cuts at: 2393 2393
Size: 4214
NsiI A_TGCA'T
Cuts at: 1044 1044
Size: 4214
NspI r_CATG'y
Cuts at: 216 1156 1267 1767 2674 216
Size: 940 111 500 907 1756
Fragments arranged by size:
1756 940 907 500 111
PciI A'CATG_T
Cuts at: 1152 2670 1152
Size: 1518 2696
PflMI CCAn_nnn'nTGG
Cuts at: 387 644 387
Size: 257 3957
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PmlI CAC'GTG
Cuts at: 281 281
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 4182
PpuMI rG'GwC_Cy
Cuts at: 127 421 456 127
Size: 294 35 3885
Fragments arranged by size:
3885 294 35
PstI C_TGCA'G
Cuts at: 320 1261 1946 320
Size: 941 685 2588
Fragments arranged by size:
2588 941 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 678 753 1639 1999 678
Size: 75 886 360 2893
Fragments arranged by size:
2893 886 360 75
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 1228 1228
Size: 4214
SalI G'TCGA_C
Cuts at: 1251 1251
Size: 4214
SapI GCTCTTCn'nnn_
Cuts at: 443 796 2237 2447 443
Size: 353 1441 210 2210
Fragments arranged by size:
2210 1441 353 210
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 3731 3731
Size: 4214
SfcI C'TryA_G
Cuts at: 33 316 1257 1942 2935 3126 33
Size: 283 941 685 993 191 1121
Fragments arranged by size:
1121 993 941 685 283 191
SfoI GGC'GCC
Cuts at: 1161 1646 1894 1161
Size: 485 248 3481
Fragments arranged by size:
3481 485 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 256 982 1236 256
Size: 726 254 3234
Fragments arranged by size:
3234 726 254
SmlI C'TyrA_G
Cuts at: 618 1369 2284 2776 3038 618
Size: 751 915 492 262 1794
Fragments arranged by size:
1794 915 751 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4214
SphI G_CATG'C
Cuts at: 216 1267 1767 216
Size: 1051 500 2663
Fragments arranged by size:
2663 1051 500
StuI AGG'CCT
Cuts at: 298 863 298
Size: 565 3649
StyI C'CwwG_G
Cuts at: 107 404 580 626 1122 1348 1702 107
Size: 297 176 46 496 226 354 2619
Fragments arranged by size:
2619 496 354 297 226 176 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1797 1930 2389 2598 1797
Size: 133 459 209 3413
Fragments arranged by size:
3413 459 209 133
TatI w'GTAC_w
Cuts at: 3729 3729
Size: 4214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 713 745 3731 3763 713
Size: 32 2986 32 1164
Fragments arranged by size:
2986 1164 32 32
Tth111I GACn'n_nGTC
Cuts at: 2011 2011
Size: 4214
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1709 1709
Size: 4214
XmaI C'CCGG_G
Cuts at: 254 980 1234 254
Size: 726 254 3234
Fragments arranged by size:
3234 726 254
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PfoI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI