Restriction map for
pF1KB3640
(Circular) (Six-base) MAPSORT of: pF1KB3640.seq Check: 4445 from: 1 to: 4370
pF1KB3640 4370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2627 2980 3642 2195
Size: 432 353 662 2923
Fragments arranged by size:
2923 662 432 353
AflIII A'CryG_T
Cuts at: 1722 3522 3803 1722
Size: 1800 281 2289
Fragments arranged by size:
2289 1800 281
AleI CACnn'nnGTG
Cuts at: 1069 1069
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 1675 3113 1675
Size: 1438 2932
ApaLI G'TGCA_C
Cuts at: 3208 3208
Size: 4370
ApoI r'AATT_y
Cuts at: 983 1374 3723 3979 983
Size: 391 2349 256 1374
Fragments arranged by size:
2349 1374 391 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 524 1390 524
Size: 866 3504
AvrII C'CTAG_G
Cuts at: 789 789
Size: 4370
BamHI G'GATC_C
Cuts at: 115 1395 115
Size: 1280 3090
BanI G'GyrC_C
Cuts at: 259 1386 1800 2048 2083 259
Size: 1127 414 248 35 2546
Fragments arranged by size:
2546 1127 414 248 35
BanII G_rGCy'C
Cuts at: 705 1384 2414 705
Size: 679 1030 2661
Fragments arranged by size:
2661 1030 679
BbeI G_GCGC'C
Cuts at: 263 1804 2052 263
Size: 1541 248 2581
Fragments arranged by size:
2581 1541 248
BbvCI CC'TCA_GC
Cuts at: 768 768
Size: 4370
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3324 4370 2268
Size: 1056 1046 2268
Fragments arranged by size:
2268 1056 1046
BclI T'GATC_A
Cuts at: 882 1890 882
Size: 1008 3362
BglII A'GATC_T
Cuts at: 844 1047 1885 3922 844
Size: 203 838 2037 1292
Fragments arranged by size:
2037 1292 838 203
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 525 1995 2088 3212 525
Size: 1470 93 1124 1683
Fragments arranged by size:
1683 1470 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4142 1582
Size: 81 335 2144 1810
Fragments arranged by size:
2144 1810 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 689 1052 175
Size: 514 363 3493
Fragments arranged by size:
3493 514 363
Bpu10I CC'TnA_GC
Cuts at: 768 768
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1129 1546 2425 3139 3437 250
Size: 879 417 879 714 298 1183
Fragments arranged by size:
1183 879 879 714 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 935 4074 935
Size: 3139 1231
BsaAI yAC'GTr
Cuts at: 592 1723 592
Size: 1131 3239
BsaBI GATnn'nnATC
Cuts at: 6 1103 1884 3921 6
Size: 1097 781 2037 455
Fragments arranged by size:
2037 1097 781 455
BsaHI Gr'CG_yC
Cuts at: 260 1801 2049 2751 4247 260
Size: 1541 248 702 1496 383
Fragments arranged by size:
1541 1496 702 383 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3169 3316 3590 1559
Size: 521 678 411 147 274 2339
Fragments arranged by size:
2339 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 195 1168 195
Size: 973 3397
BseYI C'CCAG_C
Cuts at: 1795 2553 3218 3626 1795
Size: 758 665 408 2539
Fragments arranged by size:
2539 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 1026 629
Size: 397 3973
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3188 104
Size: 1808 46 1230 1286
Fragments arranged by size:
1808 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1384 2162 2352 3212 1384
Size: 778 190 860 2542
Fragments arranged by size:
2542 860 778 190
BsmI GAATG_Cn'
Cuts at: 918 1022 918
Size: 104 4266
Bsp1286I G_dGCh'C
Cuts at: 525 705 1384 1995 2088 2162 2352 2414 3212
Size: 180 679 611 93 74 190 62 798
Cuts at: 3212 525
Size: 1683
Fragments arranged by size:
1683 798 679 611 190 180 93 74
62
BspHI T'CATG_A
Cuts at: 558 963 2802 558
Size: 405 1839 2126
Fragments arranged by size:
2126 1839 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 168 1420 1634 1936 2317 168
Size: 1252 214 302 381 2221
Fragments arranged by size:
2221 1252 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2662 2716 4200 4365 50
Size: 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 2368 2549 2368
Size: 181 4189
BssSI C'ACGA_G
Cuts at: 2641 3349 2641
Size: 708 3662
BstAPI GCAn_nnn'nTGC
Cuts at: 276 1747 276
Size: 1471 2899
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstYI r'GATC_y
Cuts at: 1 115 844 1047 1395 1862 1885 2220 2466
Size: 114 729 203 348 467 23 335 246
Cuts at: 2466 2772 2784 2870 3922 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 729 467 449 348 335 306 246
203 114 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 911 1613 911
Size: 702 3668
DraI TTT'AAA
Cuts at: 627 1369 627
Size: 742 3628
DrdI GACnn_nn'nnGTC
Cuts at: 2076 3420 2076
Size: 1344 3026
EaeI y'GGCC_r
Cuts at: 1955 2129 2520 2547 3892 1955
Size: 174 391 27 1345 2433
Fragments arranged by size:
2433 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 1112 2393 2603 1112
Size: 1281 210 2879
Fragments arranged by size:
2879 1281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 2578 3320 3466 4180 198
Size: 2380 742 146 714 388
Fragments arranged by size:
2380 742 714 388 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 689 1052 2195 2627 2980 3642 175
Size: 514 363 1143 432 353 662 903
Fragments arranged by size:
1143 903 662 514 432 363 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1211 1335 1211
Size: 124 4246
EcoO109I rG'GnC_Cy
Cuts at: 540 1509 540
Size: 969 3401
EcoRI G'AATT_C
Cuts at: 983 1374 983
Size: 391 3979
EcoRV GAT'ATC
Cuts at: 1345 1345
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 2389 291
Size: 2098 2272
FspI TGC'GCA
Cuts at: 1262 2151 1262
Size: 889 3481
HaeII r_GCGC'y
Cuts at: 263 346 1804 2052 3282 4053 263
Size: 83 1458 248 1230 771 580
Fragments arranged by size:
1458 1230 771 580 248 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 932 964 1205 1237 1329 1361 2212 2242 2244
Size: 32 241 32 92 32 851 30 2
Cuts at: 2244 2274 932
Size: 30 3028
Fragments arranged by size:
3028 851 241 92 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
KasI G'GCGC_C
Cuts at: 259 1800 2048 259
Size: 1541 248 2581
Fragments arranged by size:
2581 1541 248
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3129 3313 3129
Size: 184 4186
MscI TGG'CCA
Cuts at: 2131 2131
Size: 4370
MslI CAynn'nnrTG
Cuts at: 505 1069 2359 2486 505
Size: 564 1290 127 2389
Fragments arranged by size:
2389 1290 564 127
MspA1I CmG'CkG
Cuts at: 772 1323 1481 1795 2155 2939 3184 772
Size: 551 158 314 360 784 245 1958
Fragments arranged by size:
1958 784 551 360 314 245 158
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 260 1801 2049 260
Size: 1541 248 2581
Fragments arranged by size:
2581 1541 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4370
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NspI r_CATG'y
Cuts at: 960 1423 1923 3526 960
Size: 463 500 1603 1804
Fragments arranged by size:
1804 1603 500 463
PciI A'CATG_T
Cuts at: 3522 3522
Size: 4370
PfoI T'CCnGG_A
Cuts at: 446 446
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 4338
PstI C_TGCA'G
Cuts at: 749 1417 2102 749
Size: 668 685 3017
Fragments arranged by size:
3017 685 668
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 772 1795 2155 772
Size: 1023 360 2987
Fragments arranged by size:
2987 1023 360
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 745 1413 2098 3066 3257 33
Size: 712 668 685 968 191 1146
Fragments arranged by size:
1146 968 712 685 668 191
SfoI GGC'GCC
Cuts at: 261 1802 2050 261
Size: 1541 248 2581
Fragments arranged by size:
2581 1541 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 265 1108 1525 2440 3154 3416 265
Size: 843 417 915 714 262 1219
Fragments arranged by size:
1219 915 843 714 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 960 1423 1923 960
Size: 463 500 3407
Fragments arranged by size:
3407 500 463
StuI AGG'CCT
Cuts at: 160 160
Size: 4370
StyI C'CwwG_G
Cuts at: 107 789 1504 1858 107
Size: 682 715 354 2619
Fragments arranged by size:
2619 715 682 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1953 2086 2545 2754 1953
Size: 133 459 209 3569
Fragments arranged by size:
3569 459 209 133
TatI w'GTAC_w
Cuts at: 3885 3885
Size: 4370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 903 1865 903
Size: 962 3408
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI