Restriction map for
pF1KB3641
(Circular) (Six-base) MAPSORT of: pF1KB3641.seq Check: 1763 from: 1 to: 4055
pF1KB3641 4055 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 566 968 1093 566
Size: 402 125 3528
Fragments arranged by size:
3528 402 125
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1880 2312 2665 3327 317
Size: 1563 432 353 662 1045
Fragments arranged by size:
1563 1045 662 432 353
AflIII A'CryG_T
Cuts at: 1407 3207 3488 1407
Size: 1800 281 1974
Fragments arranged by size:
1974 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 141 141
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 223 738 1360 2798 223
Size: 515 622 1438 1480
Fragments arranged by size:
1480 1438 622 515
ApaLI G'TGCA_C
Cuts at: 2893 2893
Size: 4055
ApoI r'AATT_y
Cuts at: 922 937 1003 1059 3408 3664 922
Size: 15 66 56 2349 256 1313
Fragments arranged by size:
2349 1313 256 66 56 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
AvrII C'CTAG_G
Cuts at: 138 138
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 1071 1485 1733 1768 1071
Size: 414 248 35 3358
Fragments arranged by size:
3358 414 248 35
BanII G_rGCy'C
Cuts at: 1069 2099 1069
Size: 1030 3025
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BciVI GTATCCnnnnn_n'
Cuts at: 1953 3009 4055 1953
Size: 1056 1046 1953
Fragments arranged by size:
1953 1056 1046
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglI GCCn_nnn'nGGC
Cuts at: 140 140
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 1680 1773 2897 1680
Size: 93 1124 2838
Fragments arranged by size:
2838 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1267 1348 1683 3827 1267
Size: 81 335 2144 1495
Fragments arranged by size:
2144 1495 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 533
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2824 3122 1231
Size: 879 714 298 2164
Fragments arranged by size:
2164 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 316 3759 316
Size: 3443 612
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2854 3001 3275 1244
Size: 521 678 411 147 274 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 709 709
Size: 4055
BseYI C'CCAG_C
Cuts at: 1480 2238 2903 3311 1480
Size: 758 665 408 2224
Fragments arranged by size:
2224 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2873 104
Size: 1493 46 1230 1286
Fragments arranged by size:
1493 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1069 1847 2037 2897 1069
Size: 778 190 860 2227
Fragments arranged by size:
2227 860 778 190
BsmI GAATG_Cn'
Cuts at: 288 876 288
Size: 588 3467
Bsp1286I G_dGCh'C
Cuts at: 1069 1680 1773 1847 2037 2099 2897 1069
Size: 611 93 74 190 62 798 2227
Fragments arranged by size:
2227 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1017 2487 1017
Size: 1470 2585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 533 1105 1319 1621 2002 533
Size: 572 214 302 381 2586
Fragments arranged by size:
2586 572 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 876 1967 876
Size: 1091 2964
BsrFI r'CCGG_y
Cuts at: 2053 2234 2053
Size: 181 3874
BssSI C'ACGA_G
Cuts at: 2326 3034 2326
Size: 708 3347
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstYI r'GATC_y
Cuts at: 1 1080 1547 1570 1905 2151 2457 2469 2555
Size: 1079 467 23 335 246 306 12 86
Cuts at: 2555 3607 1
Size: 1052 449
Fragments arranged by size:
1079 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 518 765 518
Size: 247 3808
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 125 1298 125
Size: 1173 2882
DraI TTT'AAA
Cuts at: 315 1054 315
Size: 739 3316
DrdI GACnn_nn'nnGTC
Cuts at: 1761 3105 1761
Size: 1344 2711
EaeI y'GGCC_r
Cuts at: 227 579 1640 1814 2205 2232 3577 227
Size: 352 1061 174 391 27 1345 705
Fragments arranged by size:
1345 1061 705 391 352 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 3005 3151 3865 2263
Size: 742 146 714 2453
Fragments arranged by size:
2453 742 714 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 533 1880 2312 2665 3327 317
Size: 216 1347 432 353 662 1045
Fragments arranged by size:
1347 1045 662 432 353 216
EcoO109I rG'GnC_Cy
Cuts at: 735 1194 735
Size: 459 3596
EcoRI G'AATT_C
Cuts at: 922 1059 922
Size: 137 3918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 343 2074 343
Size: 1731 2324
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2967 3738 1489
Size: 248 1230 771 1806
Fragments arranged by size:
1806 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 626 658 661 693 879 911 1018 1050 1897
Size: 32 3 32 186 32 107 32 847
Cuts at: 1897 1927 1929 1959 626
Size: 30 2 30 2722
Fragments arranged by size:
2722 847 186 107 32 32 32 32
30 30 3 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 365 2814 2998 365
Size: 2449 184 1422
Fragments arranged by size:
2449 1422 184
MscI TGG'CCA
Cuts at: 229 1816 229
Size: 1587 2468
MslI CAynn'nnrTG
Cuts at: 362 485 881 932 947 2044 2171 362
Size: 123 396 51 15 1097 127 2246
Fragments arranged by size:
2246 1097 396 127 123 51 15
MspA1I CmG'CkG
Cuts at: 795 1166 1480 1840 2624 2869 795
Size: 371 314 360 784 245 1981
Fragments arranged by size:
1981 784 371 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NdeI CA'TA_TG
Cuts at: 573 573
Size: 4055
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 1108 1608 3211 1108
Size: 500 1603 1952
Fragments arranged by size:
1952 1603 500
PciI A'CATG_T
Cuts at: 3207 3207
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2501 2533 2501
Size: 32 4023
PpuMI rG'GwC_Cy
Cuts at: 735 735
Size: 4055
PshAI GACnn'nnGTC
Cuts at: 609 609
Size: 4055
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 795 1480 1840 795
Size: 685 360 3010
Fragments arranged by size:
3010 685 360
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 659 1098 1783 2751 2942 33
Size: 626 439 685 968 191 1146
Fragments arranged by size:
1146 968 685 626 439 191
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 1210 2125 2839 3101 1210
Size: 915 714 262 2164
Fragments arranged by size:
2164 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 138 1189 1543 138
Size: 1051 354 2650
Fragments arranged by size:
2650 1051 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1638 1771 2230 2439 1638
Size: 133 459 209 3254
Fragments arranged by size:
3254 459 209 133
TatI w'GTAC_w
Cuts at: 3570 3570
Size: 4055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 498 530 817 849 3572 3604 498
Size: 32 287 32 2723 32 949
Fragments arranged by size:
2723 949 287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI