Restriction map for
pF1KB3649
(Circular) (Six-base) MAPSORT of: pF1KB3649.seq Check: 2966 from: 1 to: 4889
pF1KB3649 4889 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1927 1927
Size: 4889
Acc65I G'GTAC_C
Cuts at: 1905 1905
Size: 4889
AclI AA'CG_TT
Cuts at: 4729 4729
Size: 4889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1498 1559 1796 2714 3146 3499 4161 805
Size: 693 61 237 918 432 353 662 1533
Fragments arranged by size:
1533 918 693 662 432 353 237 61
AflIII A'CryG_T
Cuts at: 2241 4041 4322 2241
Size: 1800 281 2808
Fragments arranged by size:
2808 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1000 1198 2194 3632 1000
Size: 198 996 1438 2257
Fragments arranged by size:
2257 1438 996 198
ApaLI G'TGCA_C
Cuts at: 3727 3727
Size: 4889
ApoI r'AATT_y
Cuts at: 1841 1893 4242 4498 1841
Size: 52 2349 256 2232
Fragments arranged by size:
2349 2232 256 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 4889
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4889
AvaI C'yCGr_G
Cuts at: 479 958 1909 479
Size: 479 951 3459
Fragments arranged by size:
3459 951 479
BamHI G'GATC_C
Cuts at: 1914 1914
Size: 4889
BanI G'GyrC_C
Cuts at: 592 1905 2319 2567 2602 592
Size: 1313 414 248 35 2879
Fragments arranged by size:
2879 1313 414 248 35
BanII G_rGCy'C
Cuts at: 1903 2933 1903
Size: 1030 3859
BbeI G_GCGC'C
Cuts at: 2323 2571 2323
Size: 248 4641
BbsI GAAGACnn'nnnn_
Cuts at: 242 793 953 1145 1817 242
Size: 551 160 192 672 3314
Fragments arranged by size:
3314 672 551 192 160
BciVI GTATCCnnnnn_n'
Cuts at: 2787 3843 4889 2787
Size: 1056 1046 2787
Fragments arranged by size:
2787 1056 1046
BclI T'GATC_A
Cuts at: 2409 2409
Size: 4889
BglII A'GATC_T
Cuts at: 780 1335 2404 4441 780
Size: 555 1069 2037 1228
Fragments arranged by size:
2037 1228 1069 555
BlpI GC'TnA_GC
Cuts at: 1502 2001 1502
Size: 499 4390
Bme1580I G_kGCm'C
Cuts at: 2514 2607 3731 2514
Size: 93 1124 3672
Fragments arranged by size:
3672 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1026 1274 2101 2182 2517 4661 1026
Size: 248 827 81 335 2144 1254
Fragments arranged by size:
2144 1254 827 335 248 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3256 3256
Size: 4889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 278
Size: 4889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2065 2944 3658 3956 2065
Size: 879 714 298 2998
Fragments arranged by size:
2998 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4593 4593
Size: 4889
BsaAI yAC'GTr
Cuts at: 928 1098 2242 928
Size: 170 1144 3575
Fragments arranged by size:
3575 1144 170
BsaBI GATnn'nnATC
Cuts at: 6 779 2403 4440 6
Size: 773 1624 2037 455
Fragments arranged by size:
2037 1624 773 455
BsaHI Gr'CG_yC
Cuts at: 2320 2568 3270 4766 2320
Size: 248 702 1496 2443
Fragments arranged by size:
2443 1496 702 248
BsaWI w'CCGG_w
Cuts at: 950 1671 2078 2599 3277 3688 3835 4109 950
Size: 721 407 521 678 411 147 274 1730
Fragments arranged by size:
1730 721 678 521 411 407 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 243 246 273 276 1372 1402 243
Size: 3 27 3 1096 30 3730
Fragments arranged by size:
3730 1096 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 251 899 1154 1741 1744 1819 1846 251
Size: 648 255 587 3 75 27 3294
Fragments arranged by size:
3294 648 587 255 75 27 3
BseYI C'CCAG_C
Cuts at: 2314 3072 3737 4145 2314
Size: 758 665 408 3058
Fragments arranged by size:
3058 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 691
Size: 4889
BsiEI CG_ry'CG
Cuts at: 104 2431 2477 3707 104
Size: 2327 46 1230 1286
Fragments arranged by size:
2327 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1903 2681 2871 3731 1903
Size: 778 190 860 3061
Fragments arranged by size:
3061 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1903 2514 2607 2681 2871 2933 3731 1903
Size: 611 93 74 190 62 798 3061
Fragments arranged by size:
3061 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 168 3321 168
Size: 3153 1736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1939 2153 2455 2836 1939
Size: 214 302 381 3992
Fragments arranged by size:
3992 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3181 3235 4719 4884 50
Size: 3131 54 1484 165 55
Fragments arranged by size:
3131 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2801 2801
Size: 4889
BsrFI r'CCGG_y
Cuts at: 2887 3068 2887
Size: 181 4708
BssSI C'ACGA_G
Cuts at: 3160 3868 3160
Size: 708 4181
BstAPI GCAn_nnn'nTGC
Cuts at: 2266 2266
Size: 4889
BstBI TT'CG_AA
Cuts at: 3250 3250
Size: 4889
BstXI CCAn_nnnn'nTGG
Cuts at: 1016 1641 1016
Size: 625 4264
BstYI r'GATC_y
Cuts at: 1 780 990 1335 1914 2381 2404 2739 2985
Size: 779 210 345 579 467 23 335 246
Cuts at: 2985 3291 3303 3389 4441 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 779 579 467 449 345 335 306
246 210 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1124 1124
Size: 4889
BtgI C'CryG_G
Cuts at: 107 1549 107
Size: 1442 3447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2839 3020 3183 2839
Size: 181 163 4545
Fragments arranged by size:
4545 181 163
BtsI GCAGTG_nn'
Cuts at: 254 2132 254
Size: 1878 3011
DraI TTT'AAA
Cuts at: 1888 1888
Size: 4889
DrdI GACnn_nn'nnGTC
Cuts at: 2595 3939 2595
Size: 1344 3545
EaeI y'GGCC_r
Cuts at: 2474 2648 3039 3066 4411 2474
Size: 174 391 27 1345 2952
Fragments arranged by size:
2952 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2474 2474
Size: 4889
EarI CTCTTCn'nnn_
Cuts at: 221 1046 1535 1612 1679 1688 1697 1736 1748
Size: 825 489 77 67 9 9 39 12
Cuts at: 1748 1872 2912 3122 221
Size: 124 1040 210 1988
Fragments arranged by size:
1988 1040 825 489 210 124 77 67
39 12 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3097 3839 3985 4699 3097
Size: 742 146 714 3287
Fragments arranged by size:
3287 742 714 146
EcoICRI GAG'CTC
Cuts at: 1901 1901
Size: 4889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 805 1498 1559 1796 2714 3146 3499 4161
Size: 527 693 61 237 918 432 353 662
Cuts at: 4161 278
Size: 1006
Fragments arranged by size:
1006 918 693 662 527 432 353 237
61
EcoO109I rG'GnC_Cy
Cuts at: 939 1473 2028 939
Size: 534 555 3800
Fragments arranged by size:
3800 555 534
EcoRI G'AATT_C
Cuts at: 1893 1893
Size: 4889
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 2908 1765
Size: 1143 3746
FspI TGC'GCA
Cuts at: 2670 2670
Size: 4889
HaeII r_GCGC'y
Cuts at: 1548 2323 2571 3801 4572 1548
Size: 775 248 1230 771 1865
Fragments arranged by size:
1865 1230 775 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 968 1000 1193 1225 1260 1292 1372 1404 2731
Size: 32 193 32 35 32 80 32 1327
Cuts at: 2731 2761 2763 2793 968
Size: 30 2 30 3064
Fragments arranged by size:
3064 1327 193 80 35 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 864 1928 864
Size: 1064 3825
HindIII A'AGCT_T
Cuts at: 445 445
Size: 4889
KasI G'GCGC_C
Cuts at: 2319 2567 2319
Size: 248 4641
KpnI G_GTAC'C
Cuts at: 1909 1909
Size: 4889
MluI A'CGCG_T
Cuts at: 4322 4322
Size: 4889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 325 744 3648 3832 325
Size: 419 2904 184 1382
Fragments arranged by size:
2904 1382 419 184
MscI TGG'CCA
Cuts at: 2650 2650
Size: 4889
MslI CAynn'nnrTG
Cuts at: 182 2878 3005 182
Size: 2696 127 2066
Fragments arranged by size:
2696 2066 127
MspA1I CmG'CkG
Cuts at: 1537 2000 2314 2674 3458 3703 1537
Size: 463 314 360 784 245 2723
Fragments arranged by size:
2723 784 463 360 314 245
NaeI GCC'GGC
Cuts at: 3070 3070
Size: 4889
NarI GG'CG_CC
Cuts at: 2320 2568 2320
Size: 248 4641
NcoI C'CATG_G
Cuts at: 107 107
Size: 4889
NgoMIV G'CCGG_C
Cuts at: 3068 3068
Size: 4889
NspI r_CATG'y
Cuts at: 763 1942 2442 4045 763
Size: 1179 500 1603 1607
Fragments arranged by size:
1607 1603 1179 500
PciI A'CATG_T
Cuts at: 4041 4041
Size: 4889
PflMI CCAn_nnn'nTGG
Cuts at: 873 873
Size: 4889
PfoI T'CCnGG_A
Cuts at: 1197 1197
Size: 4889
PmeI GTTT'AAAC
Cuts at: 1888 1888
Size: 4889
PmlI CAC'GTG
Cuts at: 928 928
Size: 4889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 4857
PpuMI rG'GwC_Cy
Cuts at: 939 939
Size: 4889
PsiI TTA'TAA
Cuts at: 1244 1244
Size: 4889
PstI C_TGCA'G
Cuts at: 1936 2621 1936
Size: 685 4204
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4889
PvuII CAG'CTG
Cuts at: 1537 2314 2674 1537
Size: 777 360 3752
Fragments arranged by size:
3752 777 360
RsrII CG'GwC_CG
Cuts at: 3084 3084
Size: 4889
SacI G_AGCT'C
Cuts at: 1903 1903
Size: 4889
SalI G'TCGA_C
Cuts at: 1926 1926
Size: 4889
SapI GCTCTTCn'nnn_
Cuts at: 1535 2912 3122 1535
Size: 1377 210 3302
Fragments arranged by size:
3302 1377 210
SbfI CC_TGCA'GG
Cuts at: 1936 1936
Size: 4889
ScaI AGT'ACT
Cuts at: 4406 4406
Size: 4889
SfcI C'TryA_G
Cuts at: 33 1577 1932 2617 3585 3776 33
Size: 1544 355 685 968 191 1146
Fragments arranged by size:
1544 1146 968 685 355 191
SfoI GGC'GCC
Cuts at: 2321 2569 2321
Size: 248 4641
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4889
SmaI CCC'GGG
Cuts at: 1911 1911
Size: 4889
SmlI C'TyrA_G
Cuts at: 2044 2959 3673 3935 2044
Size: 915 714 262 2998
Fragments arranged by size:
2998 915 714 262
SnaBI TAC'GTA
Cuts at: 1098 1098
Size: 4889
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4889
SphI G_CATG'C
Cuts at: 1942 2442 1942
Size: 500 4389
StyI C'CwwG_G
Cuts at: 107 275 2023 2377 107
Size: 168 1748 354 2619
Fragments arranged by size:
2619 1748 354 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2472 2605 3064 3273 2472
Size: 133 459 209 4088
Fragments arranged by size:
4088 459 209 133
TatI w'GTAC_w
Cuts at: 4404 4404
Size: 4889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 246 278 4406 4438 103
Size: 32 111 32 4128 32 554
Fragments arranged by size:
4128 554 111 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 217 2686 217
Size: 2469 2420
XbaI T'CTAG_A
Cuts at: 1920 1920
Size: 4889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2384 2384
Size: 4889
XmaI C'CCGG_G
Cuts at: 1909 1909
Size: 4889
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI