Restriction map for
pF1KB3651
(Circular) (Six-base) MAPSORT of: pF1KB3651.seq Check: 2490 from: 1 to: 6125
pF1KB3651 6125 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1191 1191
Size: 6125
AccI GT'mk_AC
Cuts at: 3163 3163
Size: 6125
Acc65I G'GTAC_C
Cuts at: 3141 3141
Size: 6125
AclI AA'CG_TT
Cuts at: 5965 5965
Size: 6125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2207 2401 2521 2638 3950 4382 4735 5397
Size: 685 194 120 117 1312 432 353 662
Cuts at: 5397 1522
Size: 2250
Fragments arranged by size:
2250 1312 685 662 432 353 194 120
117
AflIII A'CryG_T
Cuts at: 1914 3477 5277 5558 1914
Size: 1563 1800 281 2481
Fragments arranged by size:
2481 1800 1563 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 266 1319 266
Size: 1053 5072
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 121 153 505 537 121
Size: 32 352 32 5709
Fragments arranged by size:
5709 352 32 32
AlwNI CAG_nnn'CTG
Cuts at: 225 1072 1759 2379 3430 4868 225
Size: 847 687 620 1051 1438 1482
Fragments arranged by size:
1482 1438 1051 847 687 620
ApaI G_GGCC'C
Cuts at: 3076 3076
Size: 6125
ApaLI G'TGCA_C
Cuts at: 1847 1990 4963 1847
Size: 143 2973 3009
Fragments arranged by size:
3009 2973 143
ApoI r'AATT_y
Cuts at: 2989 3129 5478 5734 2989
Size: 140 2349 256 3380
Fragments arranged by size:
3380 2349 256 140
AseI AT'TA_AT
Cuts at: 20 20
Size: 6125
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
AvaI C'yCGr_G
Cuts at: 1772 3145 1772
Size: 1373 4752
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1022 1055 1022
Size: 33 6092
BamHI G'GATC_C
Cuts at: 3150 3150
Size: 6125
BanI G'GyrC_C
Cuts at: 675 3141 3555 3803 3838 675
Size: 2466 414 248 35 2962
Fragments arranged by size:
2962 2466 414 248 35
BanII G_rGCy'C
Cuts at: 472 3076 3139 4169 472
Size: 2604 63 1030 2428
Fragments arranged by size:
2604 2428 1030 63
BbeI G_GCGC'C
Cuts at: 3559 3807 3559
Size: 248 5877
BbsI GAAGACnn'nnnn_
Cuts at: 1535 2750 1535
Size: 1215 4910
BbvCI CC'TCA_GC
Cuts at: 753 1451 753
Size: 698 5427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2935 2969 2935
Size: 34 6091
BciVI GTATCCnnnnn_n'
Cuts at: 4023 5079 6125 4023
Size: 1056 1046 4023
Fragments arranged by size:
4023 1056 1046
BclI T'GATC_A
Cuts at: 3645 3645
Size: 6125
BglI GCCn_nnn'nGGC
Cuts at: 1120 1813 1120
Size: 693 5432
BglII A'GATC_T
Cuts at: 3086 3640 5677 3086
Size: 554 2037 3534
Fragments arranged by size:
3534 2037 554
BlpI GC'TnA_GC
Cuts at: 1209 3237 1209
Size: 2028 4097
Bme1580I G_kGCm'C
Cuts at: 1851 1864 1994 3076 3750 3843 4967 1851
Size: 13 130 1082 674 93 1124 3009
Fragments arranged by size:
3009 1124 1082 674 130 93 13
BmrI ACTGGGnnnn_n'
Cuts at: 517 1488 3337 3418 3753 5897 517
Size: 971 1849 81 335 2144 745
Fragments arranged by size:
2144 1849 971 745 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 147 4492 147
Size: 4345 1780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 716 785 1255 1354 2084 265
Size: 451 69 470 99 730 4306
Fragments arranged by size:
4306 730 470 451 99 69
Bpu10I CC'TnA_GC
Cuts at: 159 753 1451 1577 159
Size: 594 698 126 4707
Fragments arranged by size:
4707 698 594 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 3067 3094 3301 4180 4894 5192 743
Size: 2324 27 207 879 714 298 1676
Fragments arranged by size:
2324 1676 879 714 298 207 27
BsaI GGTCTCn'nnnn_
Cuts at: 3109 5829 3109
Size: 2720 3405
BsaAI yAC'GTr
Cuts at: 3478 3478
Size: 6125
BsaBI GATnn'nnATC
Cuts at: 6 713 1306 3639 5676 6
Size: 707 593 2333 2037 455
Fragments arranged by size:
2333 2037 707 593 455
BsaHI Gr'CG_yC
Cuts at: 3556 3804 4506 6002 3556
Size: 248 702 1496 3679
Fragments arranged by size:
3679 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2874 3314 3835 4513 4924 5071 5345 2874
Size: 440 521 678 411 147 274 3654
Fragments arranged by size:
3654 678 521 440 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 505 535 1878 1908 505
Size: 30 1343 30 4722
Fragments arranged by size:
4722 1343 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1809 1932 2059 2062 2682 2950 1809
Size: 123 127 3 620 268 4984
Fragments arranged by size:
4984 620 268 127 123 3
BseYI C'CCAG_C
Cuts at: 302 440 570 1189 1343 1499 1661 2824 2917
Size: 138 130 619 154 156 162 1163 93
Cuts at: 2917 3069 3550 4308 4973 5381 302
Size: 152 481 758 665 408 1046
Fragments arranged by size:
1163 1046 758 665 619 481 408 162
156 154 152 138 130 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 1063 1127 1813 1921 238
Size: 825 64 686 108 4442
Fragments arranged by size:
4442 825 686 108 64
BsiEI CG_ry'CG
Cuts at: 104 437 3667 3713 4943 104
Size: 333 3230 46 1230 1286
Fragments arranged by size:
3230 1286 1230 333 46
BsiHKAI G_wGCw'C
Cuts at: 472 1851 1994 3139 3917 4107 4967 472
Size: 1379 143 1145 778 190 860 1630
Fragments arranged by size:
1630 1379 1145 860 778 190 143
BsmI GAATG_Cn'
Cuts at: 2181 2181
Size: 6125
Bsp1286I G_dGCh'C
Cuts at: 472 1851 1864 1994 3076 3139 3750 3843 3917
Size: 1379 13 130 1082 63 611 93 74
Cuts at: 3917 4107 4169 4967 472
Size: 190 62 798 1630
Fragments arranged by size:
1630 1379 1082 798 611 190 130 93
74 63 62 13
BspHI T'CATG_A
Cuts at: 4557 4557
Size: 6125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 576 1191 1470 3175 3389 3691 4072 576
Size: 615 279 1705 214 302 381 2629
Fragments arranged by size:
2629 1705 615 381 302 279 214
BsrBI CCG'CTC
Cuts at: 50 4417 4471 5955 6120 50
Size: 4367 54 1484 165 55
Fragments arranged by size:
4367 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2699 4037 2699
Size: 1338 4787
BsrFI r'CCGG_y
Cuts at: 1334 4123 4304 1334
Size: 2789 181 3155
Fragments arranged by size:
3155 2789 181
BssSI C'ACGA_G
Cuts at: 4396 5104 4396
Size: 708 5417
BstAPI GCAn_nnn'nTGC
Cuts at: 225 234 675 1050 1105 1989 3502 225
Size: 9 441 375 55 884 1513 2848
Fragments arranged by size:
2848 1513 884 441 375 55 9
BstBI TT'CG_AA
Cuts at: 4486 4486
Size: 6125
BstXI CCAn_nnnn'nTGG
Cuts at: 1843 1843
Size: 6125
BstYI r'GATC_y
Cuts at: 1 1226 1262 3086 3150 3617 3640 3975 4221
Size: 1225 36 1824 64 467 23 335 246
Cuts at: 4221 4527 4539 4625 5677 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1824 1225 1052 467 449 335 306 246
86 64 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 996 996
Size: 6125
BtgI C'CryG_G
Cuts at: 107 941 2246 107
Size: 834 1305 3986
Fragments arranged by size:
3986 1305 834
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4075 4256 4419 4075
Size: 181 163 5781
Fragments arranged by size:
5781 181 163
BtsI GCAGTG_nn'
Cuts at: 221 232 924 1442 1849 2199 3368 221
Size: 11 692 518 407 350 1169 2978
Fragments arranged by size:
2978 1169 692 518 407 350 11
DraI TTT'AAA
Cuts at: 3124 3124
Size: 6125
DraIII CAC_nnn'GTG
Cuts at: 973 973
Size: 6125
DrdI GACnn_nn'nnGTC
Cuts at: 3831 5175 3831
Size: 1344 4781
EaeI y'GGCC_r
Cuts at: 422 434 562 1140 3710 3884 4275 4302 5647
Size: 12 128 578 2570 174 391 27 1345
Cuts at: 5647 422
Size: 900
Fragments arranged by size:
2570 1345 900 578 391 174 128 27
12
EagI C'GGCC_G
Cuts at: 434 3710 434
Size: 3276 2849
EarI CTCTTCn'nnn_
Cuts at: 1562 2128 2614 4148 4358 1562
Size: 566 486 1534 210 3329
Fragments arranged by size:
3329 1534 566 486 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4333 5075 5221 5935 4333
Size: 742 146 714 4523
Fragments arranged by size:
4523 742 714 146
EcoICRI GAG'CTC
Cuts at: 470 3137 470
Size: 2667 3458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 716 785 1255 1354 1522 2084 2207 2401
Size: 451 69 470 99 168 562 123 194
Cuts at: 2401 2521 2638 3950 4382 4735 5397 265
Size: 120 117 1312 432 353 662 993
Fragments arranged by size:
1312 993 662 562 470 451 432 353
194 168 123 120 117 99 69
EcoNI CCTnn'n_nnAGG
Cuts at: 583 829 995 1634 583
Size: 246 166 639 5074
Fragments arranged by size:
5074 639 246 166
EcoO109I rG'GnC_Cy
Cuts at: 763 846 1464 1482 1962 3018 3073 3264 763
Size: 83 618 18 480 1056 55 191 3624
Fragments arranged by size:
3624 1056 618 480 191 83 55 18
EcoRI G'AATT_C
Cuts at: 3129 3129
Size: 6125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3061 4144 3061
Size: 1083 5042
FspI TGC'GCA
Cuts at: 3906 3906
Size: 6125
HaeII r_GCGC'y
Cuts at: 3559 3807 5037 5808 3559
Size: 248 1230 771 3876
Fragments arranged by size:
3876 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 664 696 1878 1910 2045 2047 2077 2079 2418
Size: 32 1182 32 135 2 30 2 339
Cuts at: 2418 2422 2450 2452 2454 2484 3967 3997 3999
Size: 4 28 2 2 30 1483 30 2
Cuts at: 3999 4029 664
Size: 30 2760
Fragments arranged by size:
2760 1483 1182 339 135 32 32 30
30 30 30 28 4 2 2 2
2 2
HincII GTy'rAC
Cuts at: 3164 3164
Size: 6125
KasI G'GCGC_C
Cuts at: 3555 3803 3555
Size: 248 5877
KpnI G_GTAC'C
Cuts at: 3145 3145
Size: 6125
MfeI C'AATT_G
Cuts at: 338 338
Size: 6125
MluI A'CGCG_T
Cuts at: 5558 5558
Size: 6125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 586 2440 2818 2905 4884 5068 586
Size: 1854 378 87 1979 184 1643
Fragments arranged by size:
1979 1854 1643 378 184 87
MscI TGG'CCA
Cuts at: 424 1142 3886 424
Size: 718 2744 2663
Fragments arranged by size:
2744 2663 718
MslI CAynn'nnrTG
Cuts at: 637 707 4114 4241 637
Size: 70 3407 127 2521
Fragments arranged by size:
3407 2521 127 70
MspA1I CmG'CkG
Cuts at: 240 306 570 1657 1749 1756 3236 3550 3910
Size: 66 264 1087 92 7 1480 314 360
Cuts at: 3910 4694 4939 240
Size: 784 245 1426
Fragments arranged by size:
1480 1426 1087 784 360 314 264 245
92 66 7
NaeI GCC'GGC
Cuts at: 4306 4306
Size: 6125
NarI GG'CG_CC
Cuts at: 3556 3804 3556
Size: 248 5877
NcoI C'CATG_G
Cuts at: 107 941 2246 107
Size: 834 1305 3986
Fragments arranged by size:
3986 1305 834
NgoMIV G'CCGG_C
Cuts at: 4304 4304
Size: 6125
NspI r_CATG'y
Cuts at: 1918 3178 3678 5281 1918
Size: 1260 500 1603 2762
Fragments arranged by size:
2762 1603 1260 500
PasI CC'CwG_GG
Cuts at: 600 934 1879 600
Size: 334 945 4846
Fragments arranged by size:
4846 945 334
PciI A'CATG_T
Cuts at: 1914 5277 1914
Size: 3363 2762
PflMI CCAn_nnn'nTGG
Cuts at: 1518 2016 2924 1518
Size: 498 908 4719
Fragments arranged by size:
4719 908 498
PfoI T'CCnGG_A
Cuts at: 693 801 2336 693
Size: 108 1535 4482
Fragments arranged by size:
4482 1535 108
PmeI GTTT'AAAC
Cuts at: 3124 3124
Size: 6125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 505 537 4571 4603 505
Size: 32 4034 32 2027
Fragments arranged by size:
4034 2027 32 32
PpuMI rG'GwC_Cy
Cuts at: 763 1464 1482 1962 763
Size: 701 18 480 4926
Fragments arranged by size:
4926 701 480 18
PspOMI G'GGCC_C
Cuts at: 3072 3072
Size: 6125
PstI C_TGCA'G
Cuts at: 239 663 1059 1856 3172 3857 239
Size: 424 396 797 1316 685 2507
Fragments arranged by size:
2507 1316 797 685 424 396
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6125
PvuII CAG'CTG
Cuts at: 306 570 1657 1749 1756 3550 3910 306
Size: 264 1087 92 7 1794 360 2521
Fragments arranged by size:
2521 1794 1087 360 264 92 7
RsrII CG'GwC_CG
Cuts at: 4320 4320
Size: 6125
SacI G_AGCT'C
Cuts at: 472 3139 472
Size: 2667 3458
SalI G'TCGA_C
Cuts at: 3162 3162
Size: 6125
SapI GCTCTTCn'nnn_
Cuts at: 2128 4148 4358 2128
Size: 2020 210 3895
Fragments arranged by size:
3895 2020 210
SbfI CC_TGCA'GG
Cuts at: 3172 3172
Size: 6125
ScaI AGT'ACT
Cuts at: 2512 5642 2512
Size: 3130 2995
SfcI C'TryA_G
Cuts at: 33 235 659 1055 1610 1852 2401 2598 3168
Size: 202 424 396 555 242 549 197 570
Cuts at: 3168 3853 4821 5012 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 570 555 549 424 396
242 202 197 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1813 1813
Size: 6125
SfoI GGC'GCC
Cuts at: 3557 3805 3557
Size: 248 5877
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
SmaI CCC'GGG
Cuts at: 1774 3147 1774
Size: 1373 4752
SmlI C'TyrA_G
Cuts at: 758 3082 3109 3280 4195 4909 5171 758
Size: 2324 27 171 915 714 262 1712
Fragments arranged by size:
2324 1712 915 714 262 171 27
SpeI A'CTAG_T
Cuts at: 67 2199 67
Size: 2132 3993
SphI G_CATG'C
Cuts at: 3178 3678 3178
Size: 500 5625
StuI AGG'CCT
Cuts at: 409 751 835 409
Size: 342 84 5699
Fragments arranged by size:
5699 342 84
StyI C'CwwG_G
Cuts at: 107 941 2090 2246 2964 3259 3613 107
Size: 834 1149 156 718 295 354 2619
Fragments arranged by size:
2619 1149 834 718 354 295 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 418 994 2254 2326 3708 3841 4300 4509 418
Size: 576 1260 72 1382 133 459 209 2034
Fragments arranged by size:
2034 1382 1260 576 459 209 133 72
TatI w'GTAC_w
Cuts at: 2510 5640 2510
Size: 3130 2995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1324 1356 1448 1480 1664 1696 5642 5674 1324
Size: 32 92 32 184 32 3946 32 1775
Fragments arranged by size:
3946 1775 184 92 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3922 3922
Size: 6125
XbaI T'CTAG_A
Cuts at: 2254 3156 2254
Size: 902 5223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3620 3620
Size: 6125
XmaI C'CCGG_G
Cuts at: 1772 3145 1772
Size: 1373 4752
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AscI AvrII
BfrBI BmgBI BmtI BsiWI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI