Restriction map for
pF1KB3652
(Circular) (Six-base) MAPSORT of: pF1KB3652.seq Check: 7160 from: 1 to: 5417
pF1KB3652 5417 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 422 2455 422
Size: 2033 3384
Acc65I G'GTAC_C
Cuts at: 2433 2433
Size: 5417
AclI AA'CG_TT
Cuts at: 5257 5257
Size: 5417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1979 2132 2288 2404 3242 3674 4027 4689 1979
Size: 153 156 116 838 432 353 662 2707
Fragments arranged by size:
2707 838 662 432 353 156 153 116
AflIII A'CryG_T
Cuts at: 2769 4569 4850 2769
Size: 1800 281 3336
Fragments arranged by size:
3336 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 317 317
Size: 5417
AlwNI CAG_nnn'CTG
Cuts at: 844 2722 4160 844
Size: 1878 1438 2101
Fragments arranged by size:
2101 1878 1438
ApaI G_GGCC'C
Cuts at: 1882 1882
Size: 5417
ApaLI G'TGCA_C
Cuts at: 820 4255 820
Size: 3435 1982
ApoI r'AATT_y
Cuts at: 1234 1728 2421 4770 5026 1234
Size: 494 693 2349 256 1625
Fragments arranged by size:
2349 1625 693 494 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5417
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5417
AvaI C'yCGr_G
Cuts at: 2236 2437 2236
Size: 201 5216
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 395 428 395
Size: 33 5384
BamHI G'GATC_C
Cuts at: 2442 2442
Size: 5417
BanI G'GyrC_C
Cuts at: 231 2433 2847 3095 3130 231
Size: 2202 414 248 35 2518
Fragments arranged by size:
2518 2202 414 248 35
BanII G_rGCy'C
Cuts at: 1019 1882 1957 2431 3461 1019
Size: 863 75 474 1030 2975
Fragments arranged by size:
2975 1030 863 474 75
BbeI G_GCGC'C
Cuts at: 2851 3099 2851
Size: 248 5169
BbsI GAAGACnn'nnnn_
Cuts at: 1444 1519 2066 1444
Size: 75 547 4795
Fragments arranged by size:
4795 547 75
BbvCI CC'TCA_GC
Cuts at: 2283 2283
Size: 5417
BciVI GTATCCnnnnn_n'
Cuts at: 3315 4371 5417 3315
Size: 1056 1046 3315
Fragments arranged by size:
3315 1056 1046
BclI T'GATC_A
Cuts at: 2937 2937
Size: 5417
BglI GCCn_nnn'nGGC
Cuts at: 1828 2263 1828
Size: 435 4982
BglII A'GATC_T
Cuts at: 576 2932 4969 576
Size: 2356 2037 1024
Fragments arranged by size:
2356 2037 1024
BlpI GC'TnA_GC
Cuts at: 2529 2529
Size: 5417
Bme1580I G_kGCm'C
Cuts at: 824 1393 1882 3042 3135 4259 824
Size: 569 489 1160 93 1124 1982
Fragments arranged by size:
1982 1160 1124 569 489 93
BmgBI CAC'GTC
Cuts at: 807 1860 807
Size: 1053 4364
BmrI ACTGGGnnnn_n'
Cuts at: 2629 2710 3045 5189 2629
Size: 81 335 2144 2857
Fragments arranged by size:
2857 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3784 3784
Size: 5417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1936 1988 2074 2105 2315 1936
Size: 52 86 31 210 5038
Fragments arranged by size:
5038 210 86 52 31
Bpu10I CC'TnA_GC
Cuts at: 2283 2283
Size: 5417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 2593 3472 4186 4484 234
Size: 2359 879 714 298 1167
Fragments arranged by size:
2359 1167 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 596 855 1184 2333 5121 596
Size: 259 329 1149 2788 892
Fragments arranged by size:
2788 1149 892 329 259
BsaAI yAC'GTr
Cuts at: 1548 2770 1548
Size: 1222 4195
BsaBI GATnn'nnATC
Cuts at: 6 2931 4968 6
Size: 2925 2037 455
Fragments arranged by size:
2925 2037 455
BsaHI Gr'CG_yC
Cuts at: 2848 3096 3798 5294 2848
Size: 248 702 1496 2971
Fragments arranged by size:
2971 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1004 1182 1576 2606 3127 3805 4216 4363 4637
Size: 178 394 1030 521 678 411 147 274
Cuts at: 4637 1004
Size: 1784
Fragments arranged by size:
1784 1030 678 521 411 394 274 178
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 710 740 1022 1052 1799 1829 710
Size: 30 282 30 747 30 4298
Fragments arranged by size:
4298 747 282 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1057 1933 2197 2308 1057
Size: 876 264 111 4166
Fragments arranged by size:
4166 876 264 111
BseYI C'CCAG_C
Cuts at: 426 834 2035 2253 2842 3600 4265 4673 426
Size: 408 1201 218 589 758 665 408 1170
Fragments arranged by size:
1201 1170 758 665 589 408 408 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 779 1130 779
Size: 351 5066
BsiEI CG_ry'CG
Cuts at: 104 186 2959 3005 4235 104
Size: 82 2773 46 1230 1286
Fragments arranged by size:
2773 1286 1230 82 46
BsiHKAI G_wGCw'C
Cuts at: 824 1873 2431 3209 3399 4259 824
Size: 1049 558 778 190 860 1982
Fragments arranged by size:
1982 1049 860 778 558 190
BsmI GAATG_Cn'
Cuts at: 932 932
Size: 5417
Bsp1286I G_dGCh'C
Cuts at: 824 1019 1393 1873 1882 1957 2431 3042 3135
Size: 195 374 480 9 75 474 611 93
Cuts at: 3135 3209 3399 3461 4259 824
Size: 74 190 62 798 1982
Fragments arranged by size:
1982 798 611 480 474 374 195 190
93 75 74 62 9
BspEI T'CCGG_A
Cuts at: 1004 1004
Size: 5417
BspHI T'CATG_A
Cuts at: 918 3849 918
Size: 2931 2486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2467 2681 2983 3364 2467
Size: 214 302 381 4520
Fragments arranged by size:
4520 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3709 3763 5247 5412 50
Size: 3659 54 1484 165 55
Fragments arranged by size:
3659 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 863 3329 863
Size: 2466 2951
BsrFI r'CCGG_y
Cuts at: 763 3415 3596 763
Size: 2652 181 2584
Fragments arranged by size:
2652 2584 181
BssSI C'ACGA_G
Cuts at: 122 1018 3688 4396 122
Size: 896 2670 708 1143
Fragments arranged by size:
2670 1143 896 708
BstAPI GCAn_nnn'nTGC
Cuts at: 790 844 2794 790
Size: 54 1950 3413
Fragments arranged by size:
3413 1950 54
BstBI TT'CG_AA
Cuts at: 1095 3778 1095
Size: 2683 2734
BstEII G'GTnAC_C
Cuts at: 135 135
Size: 5417
BstXI CCAn_nnnn'nTGG
Cuts at: 2052 2052
Size: 5417
BstYI r'GATC_y
Cuts at: 1 502 576 1085 1715 2442 2909 2932 3267
Size: 501 74 509 630 727 467 23 335
Cuts at: 3267 3513 3819 3831 3917 4969 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 727 630 509 501 467 449 335
306 246 86 74 23 12
BtgI C'CryG_G
Cuts at: 927 1011 1874 2306 927
Size: 84 863 432 4038
Fragments arranged by size:
4038 863 432 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1346 2336 3367 3548 3711 1346
Size: 990 1031 181 163 3052
Fragments arranged by size:
3052 1031 990 181 163
BtsI GCAGTG_nn'
Cuts at: 2660 2660
Size: 5417
ClaI AT'CG_AT
Cuts at: 1293 1293
Size: 5417
DraI TTT'AAA
Cuts at: 2416 2416
Size: 5417
DrdI GACnn_nn'nnGTC
Cuts at: 1129 3123 4467 1129
Size: 1994 1344 2079
Fragments arranged by size:
2079 1994 1344
EaeI y'GGCC_r
Cuts at: 1103 1458 3002 3176 3567 3594 4939 1103
Size: 355 1544 174 391 27 1345 1581
Fragments arranged by size:
1581 1544 1345 391 355 174 27
EagI C'GGCC_G
Cuts at: 3002 3002
Size: 5417
EarI CTCTTCn'nnn_
Cuts at: 268 941 1955 2264 2383 2395 3440 3650 268
Size: 673 1014 309 119 12 1045 210 2035
Fragments arranged by size:
2035 1045 1014 673 309 210 119 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3625 4367 4513 5227 3625
Size: 742 146 714 3815
Fragments arranged by size:
3815 742 714 146
EcoICRI GAG'CTC
Cuts at: 2429 2429
Size: 5417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1936 1979 1988 2074 2105 2132 2288 2315 2404
Size: 43 9 86 31 27 156 27 89
Cuts at: 2404 3242 3674 4027 4689 1936
Size: 838 432 353 662 2664
Fragments arranged by size:
2664 838 662 432 353 156 89 86
43 31 27 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 244 1801 244
Size: 1557 3860
EcoO109I rG'GnC_Cy
Cuts at: 979 1879 2217 2556 979
Size: 900 338 339 3840
Fragments arranged by size:
3840 900 339 338
EcoRI G'AATT_C
Cuts at: 2421 2421
Size: 5417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 320 3436 320
Size: 3116 2301
FspI TGC'GCA
Cuts at: 3198 3198
Size: 5417
HaeII r_GCGC'y
Cuts at: 895 2851 3099 4329 5100 895
Size: 1956 248 1230 771 1212
Fragments arranged by size:
1956 1230 1212 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3259 3289 3291 3321 3259
Size: 30 2 30 5355
Fragments arranged by size:
5355 30 30 2
HincII GTy'rAC
Cuts at: 2456 2456
Size: 5417
HindIII A'AGCT_T
Cuts at: 1472 1472
Size: 5417
KasI G'GCGC_C
Cuts at: 2847 3095 2847
Size: 248 5169
KpnI G_GTAC'C
Cuts at: 2437 2437
Size: 5417
MluI A'CGCG_T
Cuts at: 4850 4850
Size: 5417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1008 4176 4360 1008
Size: 3168 184 2065
Fragments arranged by size:
3168 2065 184
MscI TGG'CCA
Cuts at: 1105 3178 1105
Size: 2073 3344
MslI CAynn'nnrTG
Cuts at: 1252 3406 3533 1252
Size: 2154 127 3136
Fragments arranged by size:
3136 2154 127
MspA1I CmG'CkG
Cuts at: 1431 2287 2308 2528 2842 3202 3986 4231 1431
Size: 856 21 220 314 360 784 245 2617
Fragments arranged by size:
2617 856 784 360 314 245 220 21
NaeI GCC'GGC
Cuts at: 765 3598 765
Size: 2833 2584
NarI GG'CG_CC
Cuts at: 2848 3096 2848
Size: 248 5169
NcoI C'CATG_G
Cuts at: 927 1011 927
Size: 84 5333
NgoMIV G'CCGG_C
Cuts at: 763 3596 763
Size: 2833 2584
NspI r_CATG'y
Cuts at: 2470 2970 4573 2470
Size: 500 1603 3314
Fragments arranged by size:
3314 1603 500
PasI CC'CwG_GG
Cuts at: 1174 1174
Size: 5417
PciI A'CATG_T
Cuts at: 4569 4569
Size: 5417
PfoI T'CCnGG_A
Cuts at: 1697 1697
Size: 5417
PmeI GTTT'AAAC
Cuts at: 2416 2416
Size: 5417
PmlI CAC'GTG
Cuts at: 1548 1548
Size: 5417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 632 664 3863 3895 632
Size: 32 3199 32 2154
Fragments arranged by size:
3199 2154 32 32
PspOMI G'GGCC_C
Cuts at: 1878 1878
Size: 5417
PstI C_TGCA'G
Cuts at: 1565 2464 3149 1565
Size: 899 685 3833
Fragments arranged by size:
3833 899 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5417
PvuII CAG'CTG
Cuts at: 2287 2842 3202 2287
Size: 555 360 4502
Fragments arranged by size:
4502 555 360
RsrII CG'GwC_CG
Cuts at: 3612 3612
Size: 5417
SacI G_AGCT'C
Cuts at: 2431 2431
Size: 5417
SacII CC_GC'GG
Cuts at: 2309 2309
Size: 5417
SalI G'TCGA_C
Cuts at: 2454 2454
Size: 5417
SapI GCTCTTCn'nnn_
Cuts at: 2383 3440 3650 2383
Size: 1057 210 4150
Fragments arranged by size:
4150 1057 210
SbfI CC_TGCA'GG
Cuts at: 2464 2464
Size: 5417
ScaI AGT'ACT
Cuts at: 211 4934 211
Size: 4723 694
SfcI C'TryA_G
Cuts at: 33 1272 1561 2460 3145 4113 4304 33
Size: 1239 289 899 685 968 191 1146
Fragments arranged by size:
1239 1146 968 899 685 289 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1828 1828
Size: 5417
SfoI GGC'GCC
Cuts at: 2849 3097 2849
Size: 248 5169
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5417
SmaI CCC'GGG
Cuts at: 2439 2439
Size: 5417
SmlI C'TyrA_G
Cuts at: 213 2572 3487 4201 4463 213
Size: 2359 915 714 262 1167
Fragments arranged by size:
2359 1167 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5417
SphI G_CATG'C
Cuts at: 2470 2970 2470
Size: 500 4917
StuI AGG'CCT
Cuts at: 368 1048 368
Size: 680 4737
StyI C'CwwG_G
Cuts at: 900 927 1011 1826 2551 2905 900
Size: 27 84 815 725 354 3412
Fragments arranged by size:
3412 815 725 354 84 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 200 575 3000 3133 3592 3801 200
Size: 375 2425 133 459 209 1816
Fragments arranged by size:
2425 1816 459 375 209 133
TatI w'GTAC_w
Cuts at: 209 1151 4932 209
Size: 942 3781 694
Fragments arranged by size:
3781 942 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 796 828 1799 1831 2147 2179 4934 4966 796
Size: 32 971 32 316 32 2755 32 1247
Fragments arranged by size:
2755 1247 971 316 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3214 3214
Size: 5417
XbaI T'CTAG_A
Cuts at: 2448 2448
Size: 5417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 1771 2912 369
Size: 1402 1141 2874
Fragments arranged by size:
2874 1402 1141
XmaI C'CCGG_G
Cuts at: 2437 2437
Size: 5417
XmnI GAAnn'nnTTC
Cuts at: 2362 2362
Size: 5417
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BcgI BfrBI BmtI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI