Restriction map for
pF1KB3657
(Circular) (Six-base) MAPSORT of: pF1KB3657.seq Check: 1479 from: 1 to: 4193
pF1KB3657 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 390 390
Size: 4193
AccI GT'mk_AC
Cuts at: 953 1231 953
Size: 278 3915
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 787 2018 2450 2803 3465 619
Size: 168 1231 432 353 662 1347
Fragments arranged by size:
1347 1231 662 432 353 168
AflIII A'CryG_T
Cuts at: 252 1545 3345 3626 252
Size: 1293 1800 281 819
Fragments arranged by size:
1800 1293 819 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 759 791 759
Size: 32 4161
AlwNI CAG_nnn'CTG
Cuts at: 144 186 1498 2936 144
Size: 42 1312 1438 1401
Fragments arranged by size:
1438 1401 1312 42
ApaLI G'TGCA_C
Cuts at: 3031 3031
Size: 4193
ApoI r'AATT_y
Cuts at: 400 506 1197 3546 3802 400
Size: 106 691 2349 256 791
Fragments arranged by size:
2349 791 691 256 106
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 169 1213 169
Size: 1044 3149
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 165 205 934 1209 1623 1871 1906 165
Size: 40 729 275 414 248 35 2452
Fragments arranged by size:
2452 729 414 275 248 40 35
BanII G_rGCy'C
Cuts at: 202 678 1207 2237 202
Size: 476 529 1030 2158
Fragments arranged by size:
2158 1030 529 476
BbeI G_GCGC'C
Cuts at: 209 1627 1875 209
Size: 1418 248 2527
Fragments arranged by size:
2527 1418 248
BbsI GAAGACnn'nnnn_
Cuts at: 593 593
Size: 4193
BciVI GTATCCnnnnn_n'
Cuts at: 2091 3147 4193 2091
Size: 1056 1046 2091
Fragments arranged by size:
2091 1056 1046
BclI T'GATC_A
Cuts at: 1077 1713 1077
Size: 636 3557
BfrBI ATG'CAT
Cuts at: 702 740 702
Size: 38 4155
BglII A'GATC_T
Cuts at: 441 1708 3745 441
Size: 1267 2037 889
Fragments arranged by size:
2037 1267 889
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 170 1818 1911 3035 170
Size: 1648 93 1124 1328
Fragments arranged by size:
1648 1328 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 258 415 982 176
Size: 82 157 567 3387
Fragments arranged by size:
3387 567 157 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2248 2962 3260 1369
Size: 879 714 298 2302
Fragments arranged by size:
2302 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 395 3897 395
Size: 3502 691
BsaAI yAC'GTr
Cuts at: 253 654 1546 253
Size: 401 892 2900
Fragments arranged by size:
2900 892 401
BsaBI GATnn'nnATC
Cuts at: 6 413 1707 3744 6
Size: 407 1294 2037 455
Fragments arranged by size:
2037 1294 455 407
BsaHI Gr'CG_yC
Cuts at: 206 1624 1872 2574 4070 206
Size: 1418 248 702 1496 329
Fragments arranged by size:
1496 1418 702 329 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2992 3139 3413 1382
Size: 521 678 411 147 274 2162
Fragments arranged by size:
2162 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1618 2376 3041 3449 1618
Size: 758 665 408 2362
Fragments arranged by size:
2362 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 3011 104
Size: 1631 46 1230 1286
Fragments arranged by size:
1631 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 3035 1207
Size: 778 190 860 2365
Fragments arranged by size:
2365 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 170 202 678 1207 1818 1911 1985 2175 2237
Size: 32 476 529 611 93 74 190 62
Cuts at: 2237 3035 170
Size: 798 1328
Fragments arranged by size:
1328 798 611 529 476 190 93 74
62 32
BspHI T'CATG_A
Cuts at: 2625 2625
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 390 1243 1457 1759 2140 390
Size: 853 214 302 381 2443
Fragments arranged by size:
2443 853 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2485 2539 4023 4188 50
Size: 2435 54 1484 165 55
Fragments arranged by size:
2435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 134 2191 2372 134
Size: 2057 181 1955
Fragments arranged by size:
2057 1955 181
BssSI C'ACGA_G
Cuts at: 2464 3172 2464
Size: 708 3485
BstAPI GCAn_nnn'nTGC
Cuts at: 813 1570 813
Size: 757 3436
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstEII G'GTnAC_C
Cuts at: 420 420
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 945 945
Size: 4193
BstYI r'GATC_y
Cuts at: 1 441 1218 1685 1708 2043 2289 2595 2607
Size: 440 777 467 23 335 246 306 12
Cuts at: 2607 2693 3745 1
Size: 86 1052 449
Fragments arranged by size:
1052 777 467 449 440 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2143 2324 2487 2143
Size: 181 163 3849
Fragments arranged by size:
3849 181 163
BtsI GCAGTG_nn'
Cuts at: 266 1436 266
Size: 1170 3023
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DraIII CAC_nnn'GTG
Cuts at: 665 665
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 3243 1899
Size: 1344 2849
EaeI y'GGCC_r
Cuts at: 656 746 1064 1778 1952 2343 2370 3715 656
Size: 90 318 714 174 391 27 1345 1134
Fragments arranged by size:
1345 1134 714 391 318 174 90 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 1169 2216 2426 1169
Size: 1047 210 2936
Fragments arranged by size:
2936 1047 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 3143 3289 4003 2401
Size: 742 146 714 2591
Fragments arranged by size:
2591 742 714 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 258 415 619 787 982 2018 2450 2803
Size: 82 157 204 168 195 1036 432 353
Cuts at: 2803 3465 176
Size: 662 904
Fragments arranged by size:
1036 904 662 432 353 204 195 168
157 82
EcoO109I rG'GnC_Cy
Cuts at: 555 1098 1332 555
Size: 543 234 3416
Fragments arranged by size:
3416 543 234
EcoRI G'AATT_C
Cuts at: 400 1197 400
Size: 797 3396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 930 2212 930
Size: 1282 2911
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 209 1627 1875 3105 3876 209
Size: 1418 248 1230 771 526
Fragments arranged by size:
1418 1230 771 526 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 801 833 1168 1200 2035 2065 2067
Size: 32 57 32 335 32 835 30 2
Cuts at: 2067 2097 712
Size: 30 2808
Fragments arranged by size:
2808 835 335 57 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 758 954 1232 758
Size: 196 278 3719
Fragments arranged by size:
3719 278 196
HindIII A'AGCT_T
Cuts at: 469 469
Size: 4193
KasI G'GCGC_C
Cuts at: 205 1623 1871 205
Size: 1418 248 2527
Fragments arranged by size:
2527 1418 248
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 2952 3136 347
Size: 2605 184 1404
Fragments arranged by size:
2605 1404 184
MscI TGG'CCA
Cuts at: 658 1954 658
Size: 1296 2897
MslI CAynn'nnrTG
Cuts at: 350 632 2182 2309 350
Size: 282 1550 127 2234
Fragments arranged by size:
2234 1550 282 127
MspA1I CmG'CkG
Cuts at: 538 1003 1304 1618 1978 2762 3007 538
Size: 465 301 314 360 784 245 1724
Fragments arranged by size:
1724 784 465 360 314 301 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 206 1624 1872 206
Size: 1418 248 2527
Fragments arranged by size:
2527 1418 248
NdeI CA'TA_TG
Cuts at: 338 338
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NsiI A_TGCA'T
Cuts at: 704 742 704
Size: 38 4155
NspI r_CATG'y
Cuts at: 1024 1246 1746 3349 1024
Size: 222 500 1603 1868
Fragments arranged by size:
1868 1603 500 222
PciI A'CATG_T
Cuts at: 3345 3345
Size: 4193
PflMI CCAn_nnn'nTGG
Cuts at: 432 665 432
Size: 233 3960
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 555 1098 555
Size: 543 3650
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 311 343 311
Size: 32 4161
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 538 1618 1978 538
Size: 1080 360 2753
Fragments arranged by size:
2753 1080 360
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 952 1230 952
Size: 278 3915
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SfcI C'TryA_G
Cuts at: 33 312 1236 1921 2889 3080 33
Size: 279 924 685 968 191 1146
Fragments arranged by size:
1146 968 924 685 279 191
SfoI GGC'GCC
Cuts at: 207 1625 1873 207
Size: 1418 248 2527
Fragments arranged by size:
2527 1418 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 1348 2263 2977 3239 1348
Size: 915 714 262 2302
Fragments arranged by size:
2302 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 1327 1681 1327
Size: 354 3839
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 1909 2368 2577 1776
Size: 133 459 209 3392
Fragments arranged by size:
3392 459 209 133
TatI w'GTAC_w
Cuts at: 3708 3708
Size: 4193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI