Restriction map for
pF1KB3659
(Circular) (Six-base) MAPSORT of: pF1KB3659.seq Check: 1014 from: 1 to: 4049
pF1KB3659 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 408 749 408
Size: 341 3708
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 139 852 1874 2306 3053 3321 110
Size: 29 713 1022 432 747 268 838
Fragments arranged by size:
1022 838 747 713 432 268 29
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AhdI GACnn_n'nnGTC
Cuts at: 323 323
Size: 4049
AleI CACnn'nnGTG
Cuts at: 520 805 520
Size: 285 3764
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaI G_GGCC'C
Cuts at: 913 1008 913
Size: 95 3954
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 1053 3402 3658 1053
Size: 2349 256 1444
Fragments arranged by size:
2349 1444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 556 1069 556
Size: 513 3536
BamHI G'GATC_C
Cuts at: 958 1074 958
Size: 116 3933
BanI G'GyrC_C
Cuts at: 295 1065 1479 1727 1762 295
Size: 770 414 248 35 2582
Fragments arranged by size:
2582 770 414 248 35
BanII G_rGCy'C
Cuts at: 913 1008 1063 2093 913
Size: 95 55 1030 2869
Fragments arranged by size:
2869 1030 95 55
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BbsI GAAGACnn'nnnn_
Cuts at: 838 838
Size: 4049
BbvCI CC'TCA_GC
Cuts at: 692 692
Size: 4049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 537 571 537
Size: 34 4015
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 298 510 913 1008 1674 1767 2823 298
Size: 212 403 95 666 93 1056 1524
Fragments arranged by size:
1524 1056 666 403 212 95 93
BmgBI CAC'GTC
Cuts at: 132 825 132
Size: 693 3356
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 839 432
Size: 407 3642
Bpu10I CC'TnA_GC
Cuts at: 692 692
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1225 2104 2596 2894 700
Size: 525 879 492 298 1855
Fragments arranged by size:
1855 879 525 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 599 1480 1728 2430 3926 599
Size: 881 248 702 1496 722
Fragments arranged by size:
1496 881 722 702 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 291 509 1474 2232 2809 3305 291
Size: 218 965 758 577 496 1035
Fragments arranged by size:
1035 965 758 577 496 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 462 295
Size: 167 3882
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
BsmI GAATG_Cn'
Cuts at: 678 678
Size: 4049
Bsp1286I G_dGCh'C
Cuts at: 298 510 913 1008 1063 1674 1767 1841 2031
Size: 212 403 95 55 611 93 74 190
Cuts at: 2031 2093 2823 298
Size: 62 730 1524
Fragments arranged by size:
1524 730 611 403 212 190 95 93
74 62 55
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 665 3225 665
Size: 2560 1489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 408 749 1099 1313 1615 1996 408
Size: 341 350 214 302 381 2461
Fragments arranged by size:
2461 381 350 341 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1961 1961
Size: 4049
BsrFI r'CCGG_y
Cuts at: 355 472 787 2047 2228 2437 355
Size: 117 315 1260 181 209 1967
Fragments arranged by size:
1967 1260 315 209 181 117
BsrGI T'GTAC_A
Cuts at: 422 422
Size: 4049
BssSI C'ACGA_G
Cuts at: 271 2320 2678 271
Size: 2049 358 1642
Fragments arranged by size:
2049 1642 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstEII G'GTnAC_C
Cuts at: 498 699 498
Size: 201 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 522 807 522
Size: 285 3764
BstYI r'GATC_y
Cuts at: 1 233 958 1074 1541 1564 1899 2145 3157
Size: 232 725 116 467 23 335 246 1012
Cuts at: 3157 3243 3255 3601 1
Size: 86 12 346 449
Fragments arranged by size:
1012 725 467 449 346 335 246 232
116 86 23 12
BtgI C'CryG_G
Cuts at: 107 143 282 308 491 107
Size: 36 139 26 183 3665
Fragments arranged by size:
3665 183 139 36 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 597 1999 2180 2343 597
Size: 1402 181 163 2303
Fragments arranged by size:
2303 1402 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 353 470 1634 1808 2199 2226 3571 353
Size: 117 1164 174 391 27 1345 831
Fragments arranged by size:
1345 1164 831 391 174 117 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 2257 2567 2713 3859 214
Size: 2043 310 146 1146 404
Fragments arranged by size:
2043 1146 404 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 139 432 839 852 1874 2306 3053 3321
Size: 29 293 407 13 1022 432 747 268
Cuts at: 3321 110
Size: 838
Fragments arranged by size:
1022 838 747 432 407 293 268 29
13
EcoO109I rG'GnC_Cy
Cuts at: 325 636 909 910 1005 1188 325
Size: 311 273 1 95 183 3186
Fragments arranged by size:
3186 311 273 183 95 1
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FseI GG_CCGG'CC
Cuts at: 359 359
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 369 1483 1731 2753 3732 369
Size: 1114 248 1022 979 686
Fragments arranged by size:
1114 1022 979 686 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 309 341 1891 1921 1923 1953 309
Size: 32 1550 30 2 30 2405
Fragments arranged by size:
2405 1550 32 30 30 2
HincII GTy'rAC
Cuts at: 684 1088 684
Size: 404 3645
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 93 2720 2904 93
Size: 2627 184 1238
Fragments arranged by size:
2627 1238 184
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 148 496 520 805 2038 2165 148
Size: 348 24 285 1233 127 2032
Fragments arranged by size:
2032 1233 348 285 127 24
MspA1I CmG'CkG
Cuts at: 587 996 1160 1474 1834 2847 3092 587
Size: 409 164 314 360 1013 245 1544
Fragments arranged by size:
1544 1013 409 360 314 245 164
NaeI GCC'GGC
Cuts at: 357 474 789 2230 357
Size: 117 315 1441 2176
Fragments arranged by size:
2176 1441 315 117
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 143 308 491 107
Size: 36 165 183 3665
Fragments arranged by size:
3665 183 165 36
NgoMIV G'CCGG_C
Cuts at: 355 472 787 2228 355
Size: 117 315 1441 2176
Fragments arranged by size:
2176 1441 315 117
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PfoI T'CCnGG_A
Cuts at: 565 961 565
Size: 396 3653
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PpuMI rG'GwC_Cy
Cuts at: 325 325
Size: 4049
PspOMI G'GGCC_C
Cuts at: 909 1004 909
Size: 95 3954
PstI C_TGCA'G
Cuts at: 233 334 365 1096 1781 233
Size: 101 31 731 685 2501
Fragments arranged by size:
2501 731 685 101 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 996 1474 1834 996
Size: 478 360 3211
Fragments arranged by size:
3211 478 360
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SanDI GG'GwC_CC
Cuts at: 325 325
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 865 3566 865
Size: 2701 1348
SfcI C'TryA_G
Cuts at: 33 229 330 361 603 1092 1777 2770 2961
Size: 196 101 31 242 489 685 993 191
Cuts at: 2961 33
Size: 1121
Fragments arranged by size:
1121 993 685 489 242 196 191 101
31
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 715 1204 2119 2611 2873 715
Size: 489 915 492 262 1891
Fragments arranged by size:
1891 915 492 489 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 107 143 218 308 491 1183 1537 107
Size: 36 75 90 183 692 354 2619
Fragments arranged by size:
2619 692 354 183 90 75 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1632 1765 2224 2433 1632
Size: 133 459 209 3248
Fragments arranged by size:
3248 459 209 133
TatI w'GTAC_w
Cuts at: 422 863 3564 422
Size: 441 2701 907
Fragments arranged by size:
2701 907 441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 409 1846 409
Size: 1437 2612
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 489 1544 306
Size: 183 1055 2811
Fragments arranged by size:
2811 1055 183
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BfrBI BglI BmtI BsaXI BseRI BsiWI BsmBI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI