Restriction map for
pF1KB3666
(Circular) (Six-base) MAPSORT of: pF1KB3666.seq Check: 2676 from: 1 to: 5327
pF1KB3666 5327 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1779 1779
Size: 5327
AatII G_ACGT'C
Cuts at: 149 149
Size: 5327
AccI GT'mk_AC
Cuts at: 2365 2365
Size: 5327
Acc65I G'GTAC_C
Cuts at: 2343 2343
Size: 5327
AclI AA'CG_TT
Cuts at: 560 5167 560
Size: 4607 720
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1706 1928 2143 3152 3584 3937 4599 1706
Size: 222 215 1009 432 353 662 2434
Fragments arranged by size:
2434 1009 662 432 353 222 215
AflII C'TTAA_G
Cuts at: 541 541
Size: 5327
AflIII A'CryG_T
Cuts at: 2679 4479 4760 2679
Size: 1800 281 3246
Fragments arranged by size:
3246 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1838 1870 1838
Size: 32 5295
AlwNI CAG_nnn'CTG
Cuts at: 2005 2221 2632 4070 2005
Size: 216 411 1438 3262
Fragments arranged by size:
3262 1438 411 216
ApaLI G'TGCA_C
Cuts at: 4165 4165
Size: 5327
ApoI r'AATT_y
Cuts at: 421 464 497 740 1162 1241 2331 4680 4936
Size: 43 33 243 422 79 1090 2349 256
Cuts at: 4936 421
Size: 812
Fragments arranged by size:
2349 1090 812 422 256 243 79 43
33
AseI AT'TA_AT
Cuts at: 20 20
Size: 5327
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5327
AvaI C'yCGr_G
Cuts at: 2027 2347 2027
Size: 320 5007
BamHI G'GATC_C
Cuts at: 2352 2352
Size: 5327
BanI G'GyrC_C
Cuts at: 163 603 1767 2343 2757 3005 3040 163
Size: 440 1164 576 414 248 35 2450
Fragments arranged by size:
2450 1164 576 440 414 248 35
BanII G_rGCy'C
Cuts at: 226 653 1427 1787 2341 3371 226
Size: 427 774 360 554 1030 2182
Fragments arranged by size:
2182 1030 774 554 427 360
BbeI G_GCGC'C
Cuts at: 167 2761 3009 167
Size: 2594 248 2485
Fragments arranged by size:
2594 2485 248
BbsI GAAGACnn'nnnn_
Cuts at: 141 1549 1736 141
Size: 1408 187 3732
Fragments arranged by size:
3732 1408 187
BbvCI CC'TCA_GC
Cuts at: 1805 1805
Size: 5327
BciVI GTATCCnnnnn_n'
Cuts at: 3225 4281 5327 3225
Size: 1056 1046 3225
Fragments arranged by size:
3225 1056 1046
BclI T'GATC_A
Cuts at: 615 2847 615
Size: 2232 3095
BfrBI ATG'CAT
Cuts at: 1029 2127 1029
Size: 1098 4229
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5327
BglII A'GATC_T
Cuts at: 2842 4879 2842
Size: 2037 3290
BlpI GC'TnA_GC
Cuts at: 2439 2439
Size: 5327
Bme1580I G_kGCm'C
Cuts at: 2952 3045 4169 2952
Size: 93 1124 4110
Fragments arranged by size:
4110 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2539 2620 2955 5099 2539
Size: 81 335 2144 2767
Fragments arranged by size:
2767 2144 335 81
BmtI G_CTAG'C
Cuts at: 1708 1708
Size: 5327
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3694 3694
Size: 5327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 1850 1899 124
Size: 1726 49 3552
Fragments arranged by size:
3552 1726 49
Bpu10I CC'TnA_GC
Cuts at: 1805 1805
Size: 5327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 2503 3382 4096 4394 363
Size: 2140 879 714 298 1296
Fragments arranged by size:
2140 1296 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 282 5031 282
Size: 4749 578
BsaAI yAC'GTr
Cuts at: 2680 2680
Size: 5327
BsaBI GATnn'nnATC
Cuts at: 6 2841 4878 6
Size: 2835 2037 455
Fragments arranged by size:
2835 2037 455
BsaHI Gr'CG_yC
Cuts at: 146 164 2758 3006 3708 5204 146
Size: 18 2594 248 702 1496 269
Fragments arranged by size:
2594 1496 702 269 248 18
BsaWI w'CCGG_w
Cuts at: 2516 3037 3715 4126 4273 4547 2516
Size: 521 678 411 147 274 3296
Fragments arranged by size:
3296 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1838 1868 1838
Size: 30 5297
BseYI C'CCAG_C
Cuts at: 1426 2752 3510 4175 4583 1426
Size: 1326 758 665 408 2170
Fragments arranged by size:
2170 1326 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2869 2915 4145 104
Size: 2765 46 1230 1286
Fragments arranged by size:
2765 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 226 653 2341 3119 3309 4169 226
Size: 427 1688 778 190 860 1384
Fragments arranged by size:
1688 1384 860 778 427 190
BsmI GAATG_Cn'
Cuts at: 1892 1892
Size: 5327
Bsp1286I G_dGCh'C
Cuts at: 226 653 1427 1787 2341 2952 3045 3119 3309
Size: 427 774 360 554 611 93 74 190
Cuts at: 3309 3371 4169 226
Size: 62 798 1384
Fragments arranged by size:
1384 798 774 611 554 427 360 190
93 74 62
BspHI T'CATG_A
Cuts at: 1452 1527 3759 1452
Size: 75 2232 3020
Fragments arranged by size:
3020 2232 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 1779 1994 2377 2591 2893 3274 263
Size: 1516 215 383 214 302 381 2316
Fragments arranged by size:
2316 1516 383 381 302 215 214
BsrBI CCG'CTC
Cuts at: 50 3619 3673 5157 5322 50
Size: 3569 54 1484 165 55
Fragments arranged by size:
3569 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3239 3239
Size: 5327
BsrFI r'CCGG_y
Cuts at: 3325 3506 3325
Size: 181 5146
BssSI C'ACGA_G
Cuts at: 1733 3598 4306 1733
Size: 1865 708 2754
Fragments arranged by size:
2754 1865 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2704 2704
Size: 5327
BstBI TT'CG_AA
Cuts at: 3688 3688
Size: 5327
BstYI r'GATC_y
Cuts at: 1 756 2352 2819 2842 3177 3423 3729 3741
Size: 755 1596 467 23 335 246 306 12
Cuts at: 3741 3827 4879 1
Size: 86 1052 449
Fragments arranged by size:
1596 1052 755 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 623 1373 623
Size: 750 4577
BtgI C'CryG_G
Cuts at: 107 824 1121 107
Size: 717 297 4313
Fragments arranged by size:
4313 717 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3277 3458 3621 3277
Size: 181 163 4983
Fragments arranged by size:
4983 181 163
BtsI GCAGTG_nn'
Cuts at: 698 2570 698
Size: 1872 3455
DraI TTT'AAA
Cuts at: 2326 2326
Size: 5327
DraIII CAC_nnn'GTG
Cuts at: 1300 1300
Size: 5327
DrdI GACnn_nn'nnGTC
Cuts at: 3033 4377 3033
Size: 1344 3983
EaeI y'GGCC_r
Cuts at: 974 2912 3086 3477 3504 4849 974
Size: 1938 174 391 27 1345 1452
Fragments arranged by size:
1938 1452 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2912 2912
Size: 5327
EarI CTCTTCn'nnn_
Cuts at: 907 1103 1415 1904 2162 3350 3560 907
Size: 196 312 489 258 1188 210 2674
Fragments arranged by size:
2674 1188 489 312 258 210 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3535 4277 4423 5137 3535
Size: 742 146 714 3725
Fragments arranged by size:
3725 742 714 146
EcoICRI GAG'CTC
Cuts at: 224 651 2339 224
Size: 427 1688 3212
Fragments arranged by size:
3212 1688 427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 1706 1850 1899 1928 2143 3152 3584 3937
Size: 1582 144 49 29 215 1009 432 353
Cuts at: 3937 4599 124
Size: 662 852
Fragments arranged by size:
1582 1009 852 662 432 353 215 144
49 29
EcoO109I rG'GnC_Cy
Cuts at: 252 2466 252
Size: 2214 3113
EcoRI G'AATT_C
Cuts at: 2331 2331
Size: 5327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1157 2173 3346 1157
Size: 1016 1173 3138
Fragments arranged by size:
3138 1173 1016
FspI TGC'GCA
Cuts at: 3108 3108
Size: 5327
HaeII r_GCGC'y
Cuts at: 118 167 2761 3009 4239 5010 118
Size: 49 2594 248 1230 771 435
Fragments arranged by size:
2594 1230 771 435 248 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3169 3199 3201 3231 3169
Size: 30 2 30 5265
Fragments arranged by size:
5265 30 30 2
HincII GTy'rAC
Cuts at: 882 2366 882
Size: 1484 3843
HindIII A'AGCT_T
Cuts at: 442 442
Size: 5327
HpaI GTT'AAC
Cuts at: 882 882
Size: 5327
KasI G'GCGC_C
Cuts at: 163 2757 3005 163
Size: 2594 248 2485
Fragments arranged by size:
2594 2485 248
KpnI G_GTAC'C
Cuts at: 2347 2347
Size: 5327
MfeI C'AATT_G
Cuts at: 1610 1610
Size: 5327
MluI A'CGCG_T
Cuts at: 4760 4760
Size: 5327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1173 1824 4086 4270 1173
Size: 651 2262 184 2230
Fragments arranged by size:
2262 2230 651 184
MscI TGG'CCA
Cuts at: 976 3088 976
Size: 2112 3215
MslI CAynn'nnrTG
Cuts at: 3316 3443 3316
Size: 127 5200
MspA1I CmG'CkG
Cuts at: 180 215 1436 1726 1795 2181 2254 2438 2752
Size: 35 1221 290 69 386 73 184 314
Cuts at: 2752 3112 3896 4141 180
Size: 360 784 245 1366
Fragments arranged by size:
1366 1221 784 386 360 314 290 245
184 73 69 35
NaeI GCC'GGC
Cuts at: 3508 3508
Size: 5327
NarI GG'CG_CC
Cuts at: 164 2758 3006 164
Size: 2594 248 2485
Fragments arranged by size:
2594 2485 248
NcoI C'CATG_G
Cuts at: 107 824 107
Size: 717 4610
NgoMIV G'CCGG_C
Cuts at: 3506 3506
Size: 5327
NheI G'CTAG_C
Cuts at: 1704 1704
Size: 5327
NsiI A_TGCA'T
Cuts at: 1031 2129 1031
Size: 1098 4229
NspI r_CATG'y
Cuts at: 2380 2880 4483 2380
Size: 500 1603 3224
Fragments arranged by size:
3224 1603 500
PciI A'CATG_T
Cuts at: 4479 4479
Size: 5327
PflMI CCAn_nnn'nTGG
Cuts at: 1300 1300
Size: 5327
PfoI T'CCnGG_A
Cuts at: 140 2303 140
Size: 2163 3164
PmeI GTTT'AAAC
Cuts at: 2326 2326
Size: 5327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1838 1870 3773 3805 1838
Size: 32 1903 32 3360
Fragments arranged by size:
3360 1903 32 32
PpuMI rG'GwC_Cy
Cuts at: 252 252
Size: 5327
PstI C_TGCA'G
Cuts at: 2374 3059 2374
Size: 685 4642
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5327
PvuII CAG'CTG
Cuts at: 180 1726 2181 2254 2752 3112 180
Size: 1546 455 73 498 360 2395
Fragments arranged by size:
2395 1546 498 455 360 73
RsrII CG'GwC_CG
Cuts at: 3522 3522
Size: 5327
SacI G_AGCT'C
Cuts at: 226 653 2341 226
Size: 427 1688 3212
Fragments arranged by size:
3212 1688 427
SalI G'TCGA_C
Cuts at: 2364 2364
Size: 5327
SapI GCTCTTCn'nnn_
Cuts at: 1415 3350 3560 1415
Size: 1935 210 3182
Fragments arranged by size:
3182 1935 210
SbfI CC_TGCA'GG
Cuts at: 2374 2374
Size: 5327
ScaI AGT'ACT
Cuts at: 4844 4844
Size: 5327
SfcI C'TryA_G
Cuts at: 33 608 1482 2370 3055 4023 4214 33
Size: 575 874 888 685 968 191 1146
Fragments arranged by size:
1146 968 888 874 685 575 191
SfoI GGC'GCC
Cuts at: 165 2759 3007 165
Size: 2594 248 2485
Fragments arranged by size:
2594 2485 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5327
SmaI CCC'GGG
Cuts at: 2349 2349
Size: 5327
SmlI C'TyrA_G
Cuts at: 378 541 2482 3397 4111 4373 378
Size: 163 1941 915 714 262 1332
Fragments arranged by size:
1941 1332 915 714 262 163
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5327
SphI G_CATG'C
Cuts at: 2380 2880 2380
Size: 500 4827
SspI AAT'ATT
Cuts at: 1177 2097 1177
Size: 920 4407
StyI C'CwwG_G
Cuts at: 107 599 824 2461 2815 107
Size: 492 225 1637 354 2619
Fragments arranged by size:
2619 1637 492 354 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 570 2910 3043 3502 3711 570
Size: 2340 133 459 209 2186
Fragments arranged by size:
2340 2186 459 209 133
TatI w'GTAC_w
Cuts at: 1495 4842 1495
Size: 3347 1980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1623 1655 4844 4876 1623
Size: 32 3189 32 2074
Fragments arranged by size:
3189 2074 32 32
Tth111I GACn'n_nGTC
Cuts at: 3124 3124
Size: 5327
XbaI T'CTAG_A
Cuts at: 1416 2358 1416
Size: 942 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1264 1542 2822 1264
Size: 278 1280 3769
Fragments arranged by size:
3769 1280 278
XmaI C'CCGG_G
Cuts at: 2347 2347
Size: 5327
XmnI GAAnn'nnTTC
Cuts at: 533 1448 533
Size: 915 4412
ZraI GAC'GTC
Cuts at: 147 147
Size: 5327
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AhdI AleI AlfI ApaI AscI AvrII
BaeI BcgI BmgBI BseRI BsgI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI NdeI NotI NruI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI