Restriction map for
pF1KB3674
(Circular) (Six-base) MAPSORT of: pF1KB3674.seq Check: 6610 from: 1 to: 4658
pF1KB3674 4658 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 206 206
Size: 4658
AccI GT'mk_AC
Cuts at: 1696 1696
Size: 4658
Acc65I G'GTAC_C
Cuts at: 1674 1674
Size: 4658
AclI AA'CG_TT
Cuts at: 4498 4498
Size: 4658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2915 3268 3930 2483
Size: 432 353 662 3211
Fragments arranged by size:
3211 662 432 353
AfeI AGC'GCT
Cuts at: 566 566
Size: 4658
AflIII A'CryG_T
Cuts at: 1378 2010 3810 4091 1378
Size: 632 1800 281 1945
Fragments arranged by size:
1945 1800 632 281
AhdI GACnn_n'nnGTC
Cuts at: 1044 1044
Size: 4658
AlwNI CAG_nnn'CTG
Cuts at: 307 477 597 896 1963 3401 307
Size: 170 120 299 1067 1438 1564
Fragments arranged by size:
1564 1438 1067 299 170 120
ApaLI G'TGCA_C
Cuts at: 3496 3496
Size: 4658
ApoI r'AATT_y
Cuts at: 1456 1662 4011 4267 1456
Size: 206 2349 256 1847
Fragments arranged by size:
2349 1847 256 206
AseI AT'TA_AT
Cuts at: 20 20
Size: 4658
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
AvaI C'yCGr_G
Cuts at: 1678 1678
Size: 4658
BamHI G'GATC_C
Cuts at: 1683 1683
Size: 4658
BanI G'GyrC_C
Cuts at: 363 1674 2088 2336 2371 363
Size: 1311 414 248 35 2650
Fragments arranged by size:
2650 1311 414 248 35
BanII G_rGCy'C
Cuts at: 996 1672 2702 996
Size: 676 1030 2952
Fragments arranged by size:
2952 1030 676
BbeI G_GCGC'C
Cuts at: 2092 2340 2092
Size: 248 4410
BbsI GAAGACnn'nnnn_
Cuts at: 579 609 579
Size: 30 4628
BciVI GTATCCnnnnn_n'
Cuts at: 2556 3612 4658 2556
Size: 1056 1046 2556
Fragments arranged by size:
2556 1056 1046
BclI T'GATC_A
Cuts at: 1127 2178 1127
Size: 1051 3607
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4658
BglII A'GATC_T
Cuts at: 2173 4210 2173
Size: 2037 2621
BlpI GC'TnA_GC
Cuts at: 1613 1770 1613
Size: 157 4501
Bme1580I G_kGCm'C
Cuts at: 1147 2283 2376 3500 1147
Size: 1136 93 1124 2305
Fragments arranged by size:
2305 1136 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1870 1951 2286 4430 1870
Size: 81 335 2144 2098
Fragments arranged by size:
2144 2098 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 583 1573 3025 583
Size: 990 1452 2216
Fragments arranged by size:
2216 1452 990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 980
Size: 4658
Bpu10I CC'TnA_GC
Cuts at: 404 1187 1325 1335 404
Size: 783 138 10 3727
Fragments arranged by size:
3727 783 138 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1834 2713 3427 3725 1834
Size: 879 714 298 2767
Fragments arranged by size:
2767 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 606 4362 606
Size: 3756 902
BsaAI yAC'GTr
Cuts at: 2011 2011
Size: 4658
BsaBI GATnn'nnATC
Cuts at: 6 2172 4209 6
Size: 2166 2037 455
Fragments arranged by size:
2166 2037 455
BsaHI Gr'CG_yC
Cuts at: 2089 2337 3039 4535 2089
Size: 248 702 1496 2212
Fragments arranged by size:
2212 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1847 2368 3046 3457 3604 3878 1847
Size: 521 678 411 147 274 2627
Fragments arranged by size:
2627 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 179 182 758 1628 179
Size: 3 576 870 3209
Fragments arranged by size:
3209 870 576 3
BseYI C'CCAG_C
Cuts at: 399 2083 2841 3506 3914 399
Size: 1684 758 665 408 1143
Fragments arranged by size:
1684 1143 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4658
BsiEI CG_ry'CG
Cuts at: 104 2200 2246 3476 104
Size: 2096 46 1230 1286
Fragments arranged by size:
2096 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1672 2450 2640 3500 1672
Size: 778 190 860 2830
Fragments arranged by size:
2830 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 768 768
Size: 4658
Bsp1286I G_dGCh'C
Cuts at: 996 1147 1672 2283 2376 2450 2640 2702 3500
Size: 151 525 611 93 74 190 62 798
Cuts at: 3500 996
Size: 2154
Fragments arranged by size:
2154 798 611 525 190 151 93 74
62
BspHI T'CATG_A
Cuts at: 3090 3090
Size: 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 206 1708 1922 2224 2605 136
Size: 70 1502 214 302 381 2189
Fragments arranged by size:
2189 1502 381 302 214 70
BsrBI CCG'CTC
Cuts at: 50 2950 3004 4488 4653 50
Size: 2900 54 1484 165 55
Fragments arranged by size:
2900 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1035 1169 2570 1035
Size: 134 1401 3123
Fragments arranged by size:
3123 1401 134
BsrFI r'CCGG_y
Cuts at: 360 2656 2837 360
Size: 2296 181 2181
Fragments arranged by size:
2296 2181 181
BsrGI T'GTAC_A
Cuts at: 252 261 252
Size: 9 4649
BssSI C'ACGA_G
Cuts at: 2929 3637 2929
Size: 708 3950
BstAPI GCAn_nnn'nTGC
Cuts at: 307 2035 307
Size: 1728 2930
BstBI TT'CG_AA
Cuts at: 3019 3019
Size: 4658
BstEII G'GTnAC_C
Cuts at: 148 148
Size: 4658
BstXI CCAn_nnnn'nTGG
Cuts at: 844 844
Size: 4658
BstYI r'GATC_y
Cuts at: 1 540 1134 1683 2150 2173 2508 2754 3060
Size: 539 594 549 467 23 335 246 306
Cuts at: 3060 3072 3158 4210 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 594 549 539 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2608 2789 2952 2608
Size: 181 163 4314
Fragments arranged by size:
4314 181 163
BtsI GCAGTG_nn'
Cuts at: 1901 1901
Size: 4658
DraI TTT'AAA
Cuts at: 1657 1657
Size: 4658
DrdI GACnn_nn'nnGTC
Cuts at: 2364 3708 2364
Size: 1344 3314
EaeI y'GGCC_r
Cuts at: 926 2243 2417 2808 2835 4180 926
Size: 1317 174 391 27 1345 1404
Fragments arranged by size:
1404 1345 1317 391 174 27
EagI C'GGCC_G
Cuts at: 2243 2243
Size: 4658
EarI CTCTTCn'nnn_
Cuts at: 282 1232 1410 2681 2891 282
Size: 950 178 1271 210 2049
Fragments arranged by size:
2049 1271 950 210 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2866 3608 3754 4468 2866
Size: 742 146 714 3056
Fragments arranged by size:
3056 742 714 146
EcoICRI GAG'CTC
Cuts at: 1670 1670
Size: 4658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 2483 2915 3268 3930 980
Size: 1503 432 353 662 1708
Fragments arranged by size:
1708 1503 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1207 1797 1207
Size: 590 4068
EcoRI G'AATT_C
Cuts at: 1662 1662
Size: 4658
EcoRV GAT'ATC
Cuts at: 1372 1372
Size: 4658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 820 1350 2677 820
Size: 530 1327 2801
Fragments arranged by size:
2801 1327 530
FspI TGC'GCA
Cuts at: 2439 2439
Size: 4658
HaeII r_GCGC'y
Cuts at: 568 1187 2092 2340 3570 4341 568
Size: 619 905 248 1230 771 885
Fragments arranged by size:
1230 905 885 771 619 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1030 1062 1119 1151 2500 2530 2532 2562 1030
Size: 32 57 32 1349 30 2 30 3126
Fragments arranged by size:
3126 1349 57 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1697 1697
Size: 4658
KasI G'GCGC_C
Cuts at: 2088 2336 2088
Size: 248 4410
KpnI G_GTAC'C
Cuts at: 1678 1678
Size: 4658
MluI A'CGCG_T
Cuts at: 4091 4091
Size: 4658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 708 1100 1518 3417 3601 708
Size: 392 418 1899 184 1765
Fragments arranged by size:
1899 1765 418 392 184
MscI TGG'CCA
Cuts at: 928 2419 928
Size: 1491 3167
MslI CAynn'nnrTG
Cuts at: 656 1381 1383 2647 2774 656
Size: 725 2 1264 127 2540
Fragments arranged by size:
2540 1264 725 127 2
MspA1I CmG'CkG
Cuts at: 250 286 304 313 388 424 477 844 1769
Size: 36 18 9 75 36 53 367 925
Cuts at: 1769 2083 2443 3227 3472 250
Size: 314 360 784 245 1436
Fragments arranged by size:
1436 925 784 367 360 314 245 75
53 36 36 18 9
NaeI GCC'GGC
Cuts at: 2839 2839
Size: 4658
NarI GG'CG_CC
Cuts at: 2089 2337 2089
Size: 248 4410
NcoI C'CATG_G
Cuts at: 107 107
Size: 4658
NdeI CA'TA_TG
Cuts at: 959 959
Size: 4658
NgoMIV G'CCGG_C
Cuts at: 2837 2837
Size: 4658
NspI r_CATG'y
Cuts at: 1382 1711 2211 3814 1382
Size: 329 500 1603 2226
Fragments arranged by size:
2226 1603 500 329
PasI CC'CwG_GG
Cuts at: 331 331
Size: 4658
PciI A'CATG_T
Cuts at: 1378 3810 1378
Size: 2432 2226
PflMI CCAn_nnn'nTGG
Cuts at: 550 597 550
Size: 47 4611
PfoI T'CCnGG_A
Cuts at: 378 620 378
Size: 242 4416
PmeI GTTT'AAAC
Cuts at: 1657 1657
Size: 4658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 4626
PpuMI rG'GwC_Cy
Cuts at: 1207 1207
Size: 4658
PstI C_TGCA'G
Cuts at: 138 312 318 393 1705 2390 138
Size: 174 6 75 1312 685 2406
Fragments arranged by size:
2406 1312 685 174 75 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4658
PvuII CAG'CTG
Cuts at: 286 313 388 424 477 844 2083 2443 286
Size: 27 75 36 53 367 1239 360 2501
Fragments arranged by size:
2501 1239 367 360 75 53 36 27
RsrII CG'GwC_CG
Cuts at: 2853 2853
Size: 4658
SacI G_AGCT'C
Cuts at: 1672 1672
Size: 4658
SalI G'TCGA_C
Cuts at: 1695 1695
Size: 4658
SapI GCTCTTCn'nnn_
Cuts at: 2681 2891 2681
Size: 210 4448
SbfI CC_TGCA'GG
Cuts at: 1705 1705
Size: 4658
ScaI AGT'ACT
Cuts at: 4175 4175
Size: 4658
SfcI C'TryA_G
Cuts at: 33 134 308 314 389 428 1701 2386 3354
Size: 101 174 6 75 39 1273 685 968
Cuts at: 3354 3545 33
Size: 191 1146
Fragments arranged by size:
1273 1146 968 685 191 174 101 75
39 6
SfoI GGC'GCC
Cuts at: 2090 2338 2090
Size: 248 4410
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
SmaI CCC'GGG
Cuts at: 1680 1680
Size: 4658
SmlI C'TyrA_G
Cuts at: 1813 2728 3442 3704 1813
Size: 915 714 262 2767
Fragments arranged by size:
2767 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4658
SphI G_CATG'C
Cuts at: 1711 2211 1711
Size: 500 4158
SspI AAT'ATT
Cuts at: 1088 1088
Size: 4658
StyI C'CwwG_G
Cuts at: 107 1792 2146 107
Size: 1685 354 2619
Fragments arranged by size:
2619 1685 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2241 2374 2833 3042 2241
Size: 133 459 209 3857
Fragments arranged by size:
3857 459 209 133
TatI w'GTAC_w
Cuts at: 252 261 660 1233 4173 252
Size: 9 399 573 2940 737
Fragments arranged by size:
2940 737 573 399 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1161 1193 1535 1567 4175 4207 1161
Size: 32 342 32 2608 32 1612
Fragments arranged by size:
2608 1612 342 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2455 2455
Size: 4658
XbaI T'CTAG_A
Cuts at: 1689 1689
Size: 4658
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1116 2153 1116
Size: 1037 3621
XmaI C'CCGG_G
Cuts at: 1678 1678
Size: 4658
XmnI GAAnn'nnTTC
Cuts at: 1271 1511 1271
Size: 240 4418
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BspEI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI