Restriction map for
pF1KB3680
(Circular) (Six-base) MAPSORT of: pF1KB3680.seq Check: 6477 from: 1 to: 5966
pF1KB3680 5966 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 3004 3004
Size: 5966
Acc65I G'GTAC_C
Cuts at: 2982 2982
Size: 5966
AclI AA'CG_TT
Cuts at: 5806 5806
Size: 5966
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 1640 2322 2834 3791 4223 4576 5238 596
Size: 1044 682 512 957 432 353 662 1324
Fragments arranged by size:
1324 1044 957 682 662 512 432 353
AflIII A'CryG_T
Cuts at: 3318 5118 5399 3318
Size: 1800 281 3885
Fragments arranged by size:
3885 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 964 964
Size: 5966
AlwNI CAG_nnn'CTG
Cuts at: 262 601 823 1689 3271 4709 262
Size: 339 222 866 1582 1438 1519
Fragments arranged by size:
1582 1519 1438 866 339 222
ApaLI G'TGCA_C
Cuts at: 4804 4804
Size: 5966
ApoI r'AATT_y
Cuts at: 2456 2638 2970 5319 5575 2456
Size: 182 332 2349 256 2847
Fragments arranged by size:
2847 2349 332 256 182
AseI AT'TA_AT
Cuts at: 20 20
Size: 5966
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
AvaI C'yCGr_G
Cuts at: 757 2986 757
Size: 2229 3737
BamHI G'GATC_C
Cuts at: 1365 2991 1365
Size: 1626 4340
BanI G'GyrC_C
Cuts at: 2982 3396 3644 3679 2982
Size: 414 248 35 5269
Fragments arranged by size:
5269 414 248 35
BanII G_rGCy'C
Cuts at: 929 1838 2878 2980 4010 929
Size: 909 1040 102 1030 2885
Fragments arranged by size:
2885 1040 1030 909 102
BbeI G_GCGC'C
Cuts at: 3400 3648 3400
Size: 248 5718
BbsI GAAGACnn'nnnn_
Cuts at: 2056 2511 2806 2056
Size: 455 295 5216
Fragments arranged by size:
5216 455 295
BciVI GTATCCnnnnn_n'
Cuts at: 3864 4920 5966 3864
Size: 1056 1046 3864
Fragments arranged by size:
3864 1056 1046
BclI T'GATC_A
Cuts at: 1412 1611 3486 1412
Size: 199 1875 3892
Fragments arranged by size:
3892 1875 199
BglII A'GATC_T
Cuts at: 1509 3481 5518 1509
Size: 1972 2037 1957
Fragments arranged by size:
2037 1972 1957
BlpI GC'TnA_GC
Cuts at: 139 413 3078 139
Size: 274 2665 3027
Fragments arranged by size:
3027 2665 274
Bme1580I G_kGCm'C
Cuts at: 3591 3684 4808 3591
Size: 93 1124 4749
Fragments arranged by size:
4749 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 300 772 844 1832 1978 3178 3259 3594 5738
Size: 472 72 988 146 1200 81 335 2144
Cuts at: 5738 300
Size: 528
Fragments arranged by size:
2144 1200 988 528 472 335 146 81
72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 759 4333 759
Size: 3574 2392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 800 1048 1187 1304 1925 2006 257
Size: 543 248 139 117 621 81 4217
Fragments arranged by size:
4217 621 543 248 139 117 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3142 4021 4735 5033 3142
Size: 879 714 298 4075
Fragments arranged by size:
4075 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 319 5670 319
Size: 5351 615
BsaAI yAC'GTr
Cuts at: 3319 3319
Size: 5966
BsaBI GATnn'nnATC
Cuts at: 6 1613 2705 3480 5517 6
Size: 1607 1092 775 2037 455
Fragments arranged by size:
2037 1607 1092 775 455
BsaHI Gr'CG_yC
Cuts at: 2922 3397 3645 4347 5843 2922
Size: 475 248 702 1496 3045
Fragments arranged by size:
3045 1496 702 475 248
BsaWI w'CCGG_w
Cuts at: 3155 3676 4354 4765 4912 5186 3155
Size: 521 678 411 147 274 3935
Fragments arranged by size:
3935 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 356 386 798 828 1979 2009 356
Size: 30 412 30 1151 30 4313
Fragments arranged by size:
4313 1151 412 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 2233 2269 583
Size: 1650 36 4280
Fragments arranged by size:
4280 1650 36
BseYI C'CCAG_C
Cuts at: 1483 2389 3391 4149 4814 5222 1483
Size: 906 1002 758 665 408 2227
Fragments arranged by size:
2227 1002 906 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 1733 1862 2590 809
Size: 924 129 728 4185
Fragments arranged by size:
4185 924 728 129
BsiEI CG_ry'CG
Cuts at: 104 3508 3554 4784 104
Size: 3404 46 1230 1286
Fragments arranged by size:
3404 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 929 1119 2980 3758 3948 4808 929
Size: 190 1861 778 190 860 2087
Fragments arranged by size:
2087 1861 860 778 190 190
BsmI GAATG_Cn'
Cuts at: 2676 2676
Size: 5966
Bsp1286I G_dGCh'C
Cuts at: 929 1119 1838 2878 2980 3591 3684 3758 3948
Size: 190 719 1040 102 611 93 74 190
Cuts at: 3948 4010 4808 929
Size: 62 798 2087
Fragments arranged by size:
2087 1040 798 719 611 190 190 102
93 74 62
BspHI T'CATG_A
Cuts at: 200 1256 4398 200
Size: 1056 3142 1768
Fragments arranged by size:
3142 1768 1056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1869 3016 3230 3532 3913 1869
Size: 1147 214 302 381 3922
Fragments arranged by size:
3922 1147 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1725 4258 4312 5796 5961 50
Size: 1675 2533 54 1484 165 55
Fragments arranged by size:
2533 1675 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 539 540 1583 3878 539
Size: 1 1043 2295 2627
Fragments arranged by size:
2627 2295 1043 1
BsrFI r'CCGG_y
Cuts at: 3964 4145 3964
Size: 181 5785
BsrGI T'GTAC_A
Cuts at: 2681 2681
Size: 5966
BssSI C'ACGA_G
Cuts at: 4237 4945 4237
Size: 708 5258
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 2008 3343 1114
Size: 894 1335 3737
Fragments arranged by size:
3737 1335 894
BstBI TT'CG_AA
Cuts at: 4327 4327
Size: 5966
BstEII G'GTnAC_C
Cuts at: 644 750 644
Size: 106 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 657 1926 657
Size: 1269 4697
BstYI r'GATC_y
Cuts at: 1 1365 1509 2109 2747 2991 3458 3481 3816
Size: 1364 144 600 638 244 467 23 335
Cuts at: 3816 4062 4368 4380 4466 5518 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1364 1052 638 600 467 449 335 306
246 244 144 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2261 2261
Size: 5966
BtgI C'CryG_G
Cuts at: 107 160 1349 1388 107
Size: 53 1189 39 4685
Fragments arranged by size:
4685 1189 53 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2039 3916 4097 4260 2039
Size: 1877 181 163 3745
Fragments arranged by size:
3745 1877 181 163
BtsI GCAGTG_nn'
Cuts at: 1404 1646 2356 2723 3209 1404
Size: 242 710 367 486 4161
Fragments arranged by size:
4161 710 486 367 242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1152 1187 1152
Size: 35 5931
DraI TTT'AAA
Cuts at: 2965 2965
Size: 5966
DrdI GACnn_nn'nnGTC
Cuts at: 2701 3672 5016 2701
Size: 971 1344 3651
Fragments arranged by size:
3651 1344 971
EaeI y'GGCC_r
Cuts at: 264 999 2243 2762 3551 3725 4116 4143 5488
Size: 735 1244 519 789 174 391 27 1345
Cuts at: 5488 264
Size: 742
Fragments arranged by size:
1345 1244 789 742 735 519 391 174
27
EagI C'GGCC_G
Cuts at: 3551 3551
Size: 5966
EarI CTCTTCn'nnn_
Cuts at: 560 728 1427 1547 2261 3989 4199 560
Size: 168 699 120 714 1728 210 2327
Fragments arranged by size:
2327 1728 714 699 210 168 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4174 4916 5062 5776 4174
Size: 742 146 714 4364
Fragments arranged by size:
4364 742 714 146
EcoICRI GAG'CTC
Cuts at: 927 2978 927
Size: 2051 3915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 596 800 1048 1187 1304 1640 1925 2006
Size: 339 204 248 139 117 336 285 81
Cuts at: 2006 2322 2834 3791 4223 4576 5238 257
Size: 316 512 957 432 353 662 985
Fragments arranged by size:
985 957 662 512 432 353 339 336
316 285 248 204 139 117 81
EcoNI CCTnn'n_nnAGG
Cuts at: 1027 1027
Size: 5966
EcoO109I rG'GnC_Cy
Cuts at: 1041 3105 1041
Size: 2064 3902
EcoRI G'AATT_C
Cuts at: 2970 2970
Size: 5966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3985 3985
Size: 5966
FspI TGC'GCA
Cuts at: 3747 3747
Size: 5966
HaeII r_GCGC'y
Cuts at: 3400 3648 4878 5649 3400
Size: 248 1230 771 3717
Fragments arranged by size:
3717 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 268 300 667 699 1222 1254 1669
Size: 32 88 32 367 32 523 32 415
Cuts at: 1669 1701 3808 3838 3840 3870 148
Size: 32 2107 30 2 30 2244
Fragments arranged by size:
2244 2107 523 415 367 88 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1330 2591 3005 1330
Size: 1261 414 4291
Fragments arranged by size:
4291 1261 414
KasI G'GCGC_C
Cuts at: 3396 3644 3396
Size: 248 5718
KpnI G_GTAC'C
Cuts at: 2986 2986
Size: 5966
MluI A'CGCG_T
Cuts at: 5399 5399
Size: 5966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 745 1449 1554 4725 4909 745
Size: 704 105 3171 184 1802
Fragments arranged by size:
3171 1802 704 184 105
MscI TGG'CCA
Cuts at: 1001 2764 3727 1001
Size: 1763 963 3240
Fragments arranged by size:
3240 1763 963
MslI CAynn'nnrTG
Cuts at: 655 1255 1924 2507 2716 3955 4082 655
Size: 600 669 583 209 1239 127 2539
Fragments arranged by size:
2539 1239 669 600 583 209 127
MspA1I CmG'CkG
Cuts at: 1984 2635 2927 3077 3391 3751 4535 4780 1984
Size: 651 292 150 314 360 784 245 3170
Fragments arranged by size:
3170 784 651 360 314 292 245 150
NaeI GCC'GGC
Cuts at: 4147 4147
Size: 5966
NarI GG'CG_CC
Cuts at: 3397 3645 3397
Size: 248 5718
NcoI C'CATG_G
Cuts at: 107 160 1349 1388 107
Size: 53 1189 39 4685
Fragments arranged by size:
4685 1189 53 39
NgoMIV G'CCGG_C
Cuts at: 4145 4145
Size: 5966
NspI r_CATG'y
Cuts at: 2076 3019 3519 5122 2076
Size: 943 500 1603 2920
Fragments arranged by size:
2920 1603 943 500
PasI CC'CwG_GG
Cuts at: 1700 1700
Size: 5966
PciI A'CATG_T
Cuts at: 5118 5118
Size: 5966
PflMI CCAn_nnn'nTGG
Cuts at: 2089 2089
Size: 5966
PfoI T'CCnGG_A
Cuts at: 1025 1025
Size: 5966
PmeI GTTT'AAAC
Cuts at: 2965 2965
Size: 5966
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 4412
Size: 32 5934
PpuMI rG'GwC_Cy
Cuts at: 1041 1041
Size: 5966
PstI C_TGCA'G
Cuts at: 1866 1983 2363 2496 2528 3013 3698 1866
Size: 117 380 133 32 485 685 4134
Fragments arranged by size:
4134 685 485 380 133 117 32
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5966
PvuII CAG'CTG
Cuts at: 1984 2635 3391 3751 1984
Size: 651 756 360 4199
Fragments arranged by size:
4199 756 651 360
RsrII CG'GwC_CG
Cuts at: 4161 4161
Size: 5966
SacI G_AGCT'C
Cuts at: 929 2980 929
Size: 2051 3915
SalI G'TCGA_C
Cuts at: 3003 3003
Size: 5966
SapI GCTCTTCn'nnn_
Cuts at: 1547 3989 4199 1547
Size: 2442 210 3314
Fragments arranged by size:
3314 2442 210
SbfI CC_TGCA'GG
Cuts at: 3013 3013
Size: 5966
ScaI AGT'ACT
Cuts at: 310 1977 5483 310
Size: 1667 3506 793
Fragments arranged by size:
3506 1667 793
SfcI C'TryA_G
Cuts at: 33 593 671 1226 1862 1979 2359 2421 2492
Size: 560 78 555 636 117 380 62 71
Cuts at: 2492 2524 3009 3694 4662 4853 33
Size: 32 485 685 968 191 1146
Fragments arranged by size:
1146 968 685 636 560 555 485 380
191 117 78 71 62 32
SfoI GGC'GCC
Cuts at: 3398 3646 3398
Size: 248 5718
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
SmaI CCC'GGG
Cuts at: 759 2988 759
Size: 2229 3737
SmlI C'TyrA_G
Cuts at: 3121 4036 4750 5012 3121
Size: 915 714 262 4075
Fragments arranged by size:
4075 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5966
SphI G_CATG'C
Cuts at: 3019 3519 3019
Size: 500 5466
StuI AGG'CCT
Cuts at: 2577 2577
Size: 5966
StyI C'CwwG_G
Cuts at: 107 160 1349 1388 3100 3454 107
Size: 53 1189 39 1712 354 2619
Fragments arranged by size:
2619 1712 1189 354 53 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1095 3549 3682 4141 4350 1095
Size: 2454 133 459 209 2711
Fragments arranged by size:
2711 2454 459 209 133
TatI w'GTAC_w
Cuts at: 308 1012 1975 2681 5481 308
Size: 704 963 706 2800 793
Fragments arranged by size:
2800 963 793 706 704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 339 356 371 388 774 798 806 830 5483
Size: 17 15 17 386 24 8 24 4653
Cuts at: 5483 5515 339
Size: 32 790
Fragments arranged by size:
4653 790 386 32 24 24 17 17
15 8
Tth111I GACn'n_nGTC
Cuts at: 2863 3763 2863
Size: 900 5066
XbaI T'CTAG_A
Cuts at: 2997 2997
Size: 5966
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1356 3461 1356
Size: 2105 3861
XmaI C'CCGG_G
Cuts at: 757 2986 757
Size: 2229 3737
XmnI GAAnn'nnTTC
Cuts at: 491 2441 491
Size: 1950 4016
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmBI BspEI BssHII BstZ17I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI