Restriction map for
pF1KB3681
(Circular) (Six-base) MAPSORT of: pF1KB3681.seq Check: 2233 from: 1 to: 5798
pF1KB3681 5798 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 2836 2836
Size: 5798
Acc65I G'GTAC_C
Cuts at: 2814 2814
Size: 5798
AclI AA'CG_TT
Cuts at: 5638 5638
Size: 5798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1645 1996 2595 3623 4055 4408 5070 580
Size: 1065 351 599 1028 432 353 662 1308
Fragments arranged by size:
1308 1065 1028 662 599 432 353 351
AfeI AGC'GCT
Cuts at: 1903 1903
Size: 5798
AflII C'TTAA_G
Cuts at: 1702 1702
Size: 5798
AflIII A'CryG_T
Cuts at: 2678 3150 4950 5231 2678
Size: 472 1800 281 3245
Fragments arranged by size:
3245 1800 472 281
AgeI A'CCGG_T
Cuts at: 2730 2730
Size: 5798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1601 2407 1601
Size: 806 4992
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1285 1317 1323 1324 1355 1356 1285
Size: 32 6 1 31 1 5727
Fragments arranged by size:
5727 32 31 6 1 1
AlwNI CAG_nnn'CTG
Cuts at: 802 1037 1992 2362 3103 4541 802
Size: 235 955 370 741 1438 2059
Fragments arranged by size:
2059 1438 955 741 370 235
ApaI G_GGCC'C
Cuts at: 372 577 923 1022 1049 2217 2696 372
Size: 205 346 99 27 1168 479 3474
Fragments arranged by size:
3474 1168 479 346 205 99 27
ApaLI G'TGCA_C
Cuts at: 2253 4636 2253
Size: 2383 3415
ApoI r'AATT_y
Cuts at: 2802 5151 5407 2802
Size: 2349 256 3193
Fragments arranged by size:
3193 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5798
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
AvaI C'yCGr_G
Cuts at: 126 683 877 923 1909 2542 2688 2780 2818
Size: 557 194 46 986 633 146 92 38
Cuts at: 2818 126
Size: 3106
Fragments arranged by size:
3106 986 633 557 194 146 92 46
38
BamHI G'GATC_C
Cuts at: 276 434 2823 276
Size: 158 2389 3251
Fragments arranged by size:
3251 2389 158
BanI G'GyrC_C
Cuts at: 133 178 567 588 939 2079 2159 2283 2727
Size: 45 389 21 351 1140 80 124 444
Cuts at: 2727 2814 3228 3476 3511 133
Size: 87 414 248 35 2420
Fragments arranged by size:
2420 1140 444 414 389 351 248 124
87 80 45 35 21
BanII G_rGCy'C
Cuts at: 126 152 250 372 400 577 644 923 1022
Size: 26 98 122 28 177 67 279 99
Cuts at: 1022 1049 1250 1682 1858 1916 2135 2217 2484
Size: 27 201 432 176 58 219 82 267
Cuts at: 2484 2696 2812 3842 126
Size: 212 116 1030 2082
Fragments arranged by size:
2082 1030 432 279 267 219 212 201
177 176 122 116 99 98 82 67
58 28 27 26
BbeI G_GCGC'C
Cuts at: 137 182 3232 3480 137
Size: 45 3050 248 2455
Fragments arranged by size:
3050 2455 248 45
BbsI GAAGACnn'nnnn_
Cuts at: 2042 2568 2042
Size: 526 5272
BbvCI CC'TCA_GC
Cuts at: 2599 2599
Size: 5798
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1562 1596 90
Size: 34 1438 34 4292
Fragments arranged by size:
4292 1438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 750 3696 4752 5798 750
Size: 2946 1056 1046 750
Fragments arranged by size:
2946 1056 1046 750
BclI T'GATC_A
Cuts at: 3318 3318
Size: 5798
BfrBI ATG'CAT
Cuts at: 111 1614 111
Size: 1503 4295
BglI GCCn_nnn'nGGC
Cuts at: 2359 2666 2359
Size: 307 5491
BglII A'GATC_T
Cuts at: 3313 5350 3313
Size: 2037 3761
BlpI GC'TnA_GC
Cuts at: 2910 2910
Size: 5798
Bme1580I G_kGCm'C
Cuts at: 372 570 577 593 869 923 944 1022 1049
Size: 198 7 16 276 54 21 78 27
Cuts at: 1049 1181 1311 1781 1821 2082 2217 2257 2696
Size: 132 130 470 40 261 135 40 439
Cuts at: 2696 3423 3516 4640 372
Size: 727 93 1124 1530
Fragments arranged by size:
1530 1124 727 470 439 276 261 198
135 132 130 93 78 54 40 40
27 21 16 7
BmrI ACTGGGnnnn_n'
Cuts at: 276 344 719 1168 1956 3010 3091 3426 5570
Size: 68 375 449 788 1054 81 335 2144
Cuts at: 5570 276
Size: 504
Fragments arranged by size:
2144 1054 788 504 449 375 335 81
68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4165 4165
Size: 5798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 305 412 658 1144 1471 1573 1900 2014
Size: 72 107 246 486 327 102 327 114
Cuts at: 2014 2362 233
Size: 348 3669
Fragments arranged by size:
3669 486 348 327 327 246 114 107
102 72
Bpu10I CC'TnA_GC
Cuts at: 249 2599 249
Size: 2350 3448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 1715 2974 3853 4567 4865 1474
Size: 241 1259 879 714 298 2407
Fragments arranged by size:
2407 1259 879 714 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 988 5502 988
Size: 4514 1284
BsaAI yAC'GTr
Cuts at: 3151 3151
Size: 5798
BsaBI GATnn'nnATC
Cuts at: 6 3312 5349 6
Size: 3306 2037 455
Fragments arranged by size:
3306 2037 455
BsaHI Gr'CG_yC
Cuts at: 134 179 3229 3477 4179 5675 134
Size: 45 3050 248 702 1496 257
Fragments arranged by size:
3050 1496 702 257 248 45
BsaWI w'CCGG_w
Cuts at: 431 2730 2987 3508 4186 4597 4744 5018 431
Size: 2299 257 521 678 411 147 274 1211
Fragments arranged by size:
2299 1211 678 521 411 274 257 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1369 1399 2224 2254 1369
Size: 30 825 30 4913
Fragments arranged by size:
4913 825 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1006 1141 1144 1156 1159 1162 1666 1945 1948
Size: 135 3 12 3 3 504 279 3
Cuts at: 1948 2737 1006
Size: 789 4067
Fragments arranged by size:
4067 789 504 279 135 12 3 3
3 3
BseYI C'CCAG_C
Cuts at: 497 601 808 869 1024 2321 2501 3223 3981
Size: 104 207 61 155 1297 180 722 758
Cuts at: 3981 4646 5054 497
Size: 665 408 1241
Fragments arranged by size:
1297 1241 758 722 665 408 207 180
155 104 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1237 1423 1480 2377 403
Size: 834 186 57 897 3824
Fragments arranged by size:
3824 897 834 186 57
BsiEI CG_ry'CG
Cuts at: 104 1352 1761 3340 3386 4616 104
Size: 1248 409 1579 46 1230 1286
Fragments arranged by size:
1579 1286 1248 1230 409 46
BsiHKAI G_wGCw'C
Cuts at: 1250 1580 1682 1858 1916 2203 2257 2812 3590
Size: 330 102 176 58 287 54 555 778
Cuts at: 3590 3780 4640 1250
Size: 190 860 2408
Fragments arranged by size:
2408 860 778 555 330 287 190 176
102 58 54
BsmI GAATG_Cn'
Cuts at: 1590 1616 1590
Size: 26 5772
Bsp1286I G_dGCh'C
Cuts at: 126 152 250 372 400 570 577 593 644
Size: 26 98 122 28 170 7 16 51
Cuts at: 644 869 923 944 1022 1049 1181 1250 1311
Size: 225 54 21 78 27 132 69 61
Cuts at: 1311 1580 1682 1781 1821 1858 1916 2082 2135
Size: 269 102 99 40 37 58 166 53
Cuts at: 2135 2203 2217 2257 2484 2696 2812 3423 3516
Size: 68 14 40 227 212 116 611 93
Cuts at: 3516 3590 3780 3842 4640 126
Size: 74 190 62 798 1284
Fragments arranged by size:
1284 798 611 269 227 225 212 190
170 166 132 122 116 102 99 98
93 78 74 69 68 62 61 58
54 53 51 40 40 37 28 27
26 21 16 14 7
BspHI T'CATG_A
Cuts at: 4230 4230
Size: 5798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 617 1006 2848 3062 3364 3745 617
Size: 389 1842 214 302 381 2670
Fragments arranged by size:
2670 1842 389 381 302 214
BsrBI CCG'CTC
Cuts at: 50 184 2240 2556 4090 4144 5628 5793 50
Size: 134 2056 316 1534 54 1484 165 55
Fragments arranged by size:
2056 1534 1484 316 165 134 55 54
BsrDI GCAATG_nn'
Cuts at: 1235 1432 3710 1235
Size: 197 2278 3323
Fragments arranged by size:
3323 2278 197
BsrFI r'CCGG_y
Cuts at: 189 1091 2730 3796 3977 189
Size: 902 1639 1066 181 2010
Fragments arranged by size:
2010 1639 1066 902 181
BssHII G'CGCG_C
Cuts at: 238 238
Size: 5798
BssSI C'ACGA_G
Cuts at: 4069 4777 4069
Size: 708 5090
BstAPI GCAn_nnn'nTGC
Cuts at: 1446 1620 2123 2174 2261 2283 3175 1446
Size: 174 503 51 87 22 892 4069
Fragments arranged by size:
4069 892 503 174 87 51 22
BstBI TT'CG_AA
Cuts at: 4159 4159
Size: 5798
BstXI CCAn_nnnn'nTGG
Cuts at: 699 1096 699
Size: 397 5401
BstYI r'GATC_y
Cuts at: 1 276 434 2064 2823 3290 3313 3648 3894
Size: 275 158 1630 759 467 23 335 246
Cuts at: 3894 4200 4212 4298 5350 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1630 1052 759 467 449 335 306 275
246 158 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2486 2486
Size: 5798
BtgI C'CryG_G
Cuts at: 1362 1515 1362
Size: 153 5645
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3748 3929 4092 3748
Size: 181 163 5454
Fragments arranged by size:
5454 181 163
BtsI GCAGTG_nn'
Cuts at: 667 2163 2750 3041 667
Size: 1496 587 291 3424
Fragments arranged by size:
3424 1496 587 291
DraI TTT'AAA
Cuts at: 2797 2797
Size: 5798
DraIII CAC_nnn'GTG
Cuts at: 2114 2283 2114
Size: 169 5629
DrdI GACnn_nn'nnGTC
Cuts at: 3504 4848 3504
Size: 1344 4454
EaeI y'GGCC_r
Cuts at: 114 161 465 689 1079 1349 1797 2658 3383
Size: 47 304 224 390 270 448 861 725
Cuts at: 3383 3557 3948 3975 5320 114
Size: 174 391 27 1345 592
Fragments arranged by size:
1345 861 725 592 448 391 390 304
270 224 174 47 27
EagI C'GGCC_G
Cuts at: 1349 3383 1349
Size: 2034 3764
EarI CTCTTCn'nnn_
Cuts at: 1148 3821 4031 1148
Size: 2673 210 2915
Fragments arranged by size:
2915 2673 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 176 4006 4748 4894 5608 176
Size: 3830 742 146 714 366
Fragments arranged by size:
3830 742 714 366 146
EcoICRI GAG'CTC
Cuts at: 1248 1680 1856 1914 2810 1248
Size: 432 176 58 896 4236
Fragments arranged by size:
4236 896 432 176 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 305 412 580 658 1144 1471 1573 1645
Size: 72 107 168 78 486 327 102 72
Cuts at: 1645 1900 1996 2014 2362 2595 3623 4055 4408
Size: 255 96 18 348 233 1028 432 353
Cuts at: 4408 5070 233
Size: 662 961
Fragments arranged by size:
1028 961 662 486 432 353 348 327
255 233 168 107 102 96 78 72
72 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1126 1294 1126
Size: 168 5630
EcoO109I rG'GnC_Cy
Cuts at: 369 493 520 696 817 920 1019 1046 2692
Size: 124 27 176 121 103 99 27 1646
Cuts at: 2692 2937 369
Size: 245 3230
Fragments arranged by size:
3230 1646 245 176 124 121 103 99
27 27
EcoRI G'AATT_C
Cuts at: 2802 2802
Size: 5798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3817 3817
Size: 5798
FspI TGC'GCA
Cuts at: 3579 3579
Size: 5798
HaeII r_GCGC'y
Cuts at: 137 182 317 1905 2328 3232 3480 4710 5481
Size: 45 135 1588 423 904 248 1230 771
Cuts at: 5481 137
Size: 454
Fragments arranged by size:
1588 1230 904 771 454 423 248 135
45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3640 3670 3672 3702 3640
Size: 30 2 30 5736
Fragments arranged by size:
5736 30 30 2
HincII GTy'rAC
Cuts at: 1113 1211 2837 1113
Size: 98 1626 4074
Fragments arranged by size:
4074 1626 98
KasI G'GCGC_C
Cuts at: 133 178 3228 3476 133
Size: 45 3050 248 2455
Fragments arranged by size:
3050 2455 248 45
KpnI G_GTAC'C
Cuts at: 2818 2818
Size: 5798
MluI A'CGCG_T
Cuts at: 5231 5231
Size: 5798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 270 1007 1883 2267 4557 4741 270
Size: 737 876 384 2290 184 1327
Fragments arranged by size:
2290 1327 876 737 384 184
MscI TGG'CCA
Cuts at: 116 467 691 1799 3559 116
Size: 351 224 1108 1760 2355
Fragments arranged by size:
2355 1760 1108 351 224
MslI CAynn'nnrTG
Cuts at: 1583 2116 3787 3914 1583
Size: 533 1671 127 3467
Fragments arranged by size:
3467 1671 533 127
MspA1I CmG'CkG
Cuts at: 501 896 956 2603 2909 3223 3583 4367 4612
Size: 395 60 1647 306 314 360 784 245
Cuts at: 4612 501
Size: 1687
Fragments arranged by size:
1687 1647 784 395 360 314 306 245
60
NaeI GCC'GGC
Cuts at: 191 3979 191
Size: 3788 2010
NarI GG'CG_CC
Cuts at: 134 179 3229 3477 134
Size: 45 3050 248 2455
Fragments arranged by size:
3050 2455 248 45
NcoI C'CATG_G
Cuts at: 1515 1515
Size: 5798
NgoMIV G'CCGG_C
Cuts at: 189 3977 189
Size: 3788 2010
NsiI A_TGCA'T
Cuts at: 113 1616 113
Size: 1503 4295
NspI r_CATG'y
Cuts at: 1449 2121 2185 2575 2851 3351 4954 1449
Size: 672 64 390 276 500 1603 2293
Fragments arranged by size:
2293 1603 672 500 390 276 64
PasI CC'CwG_GG
Cuts at: 707 1042 707
Size: 335 5463
PciI A'CATG_T
Cuts at: 4950 4950
Size: 5798
PflMI CCAn_nnn'nTGG
Cuts at: 802 1290 1449 802
Size: 488 159 5151
Fragments arranged by size:
5151 488 159
PfoI T'CCnGG_A
Cuts at: 1289 2093 2339 1289
Size: 804 246 4748
Fragments arranged by size:
4748 804 246
PmeI GTTT'AAAC
Cuts at: 2797 2797
Size: 5798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 5766
PpuMI rG'GwC_Cy
Cuts at: 493 520 817 493
Size: 27 297 5474
Fragments arranged by size:
5474 297 27
PshAI GACnn'nnGTC
Cuts at: 518 1197 518
Size: 679 5119
PspOMI G'GGCC_C
Cuts at: 368 573 919 1018 1045 2213 2692 368
Size: 205 346 99 27 1168 479 3474
Fragments arranged by size:
3474 1168 479 346 205 99 27
PspXI vC'TCGA_Gb
Cuts at: 1909 1909
Size: 5798
PstI C_TGCA'G
Cuts at: 614 735 2308 2367 2386 2845 3530 614
Size: 121 1573 59 19 459 685 2882
Fragments arranged by size:
2882 1573 685 459 121 59 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5798
PvuII CAG'CTG
Cuts at: 2603 3223 3583 2603
Size: 620 360 4818
Fragments arranged by size:
4818 620 360
RsrII CG'GwC_CG
Cuts at: 3993 3993
Size: 5798
SacI G_AGCT'C
Cuts at: 1250 1682 1858 1916 2812 1250
Size: 432 176 58 896 4236
Fragments arranged by size:
4236 896 432 176 58
SalI G'TCGA_C
Cuts at: 2835 2835
Size: 5798
SapI GCTCTTCn'nnn_
Cuts at: 3821 4031 3821
Size: 210 5588
SbfI CC_TGCA'GG
Cuts at: 2845 2845
Size: 5798
ScaI AGT'ACT
Cuts at: 5315 5315
Size: 5798
SfcI C'TryA_G
Cuts at: 33 610 731 2304 2363 2382 2632 2841 3526
Size: 577 121 1573 59 19 250 209 685
Cuts at: 3526 4494 4685 33
Size: 968 191 1146
Fragments arranged by size:
1573 1146 968 685 577 250 209 191
121 59 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 2666 2666
Size: 5798
SfoI GGC'GCC
Cuts at: 135 180 3230 3478 135
Size: 45 3050 248 2455
Fragments arranged by size:
3050 2455 248 45
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
SgrAI Cr'CCGG_yG
Cuts at: 2730 2730
Size: 5798
SmaI CCC'GGG
Cuts at: 128 925 2690 2820 128
Size: 797 1765 130 3106
Fragments arranged by size:
3106 1765 797 130
SmlI C'TyrA_G
Cuts at: 1489 1702 1730 1909 2953 3868 4582 4844 1489
Size: 213 28 179 1044 915 714 262 2443
Fragments arranged by size:
2443 1044 915 714 262 213 179 28
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5798
SphI G_CATG'C
Cuts at: 2121 2851 3351 2121
Size: 730 500 4568
Fragments arranged by size:
4568 730 500
StuI AGG'CCT
Cuts at: 889 1267 2173 2353 889
Size: 378 906 180 4334
Fragments arranged by size:
4334 906 378 180
StyI C'CwwG_G
Cuts at: 692 1076 1515 1794 2459 2932 3286 692
Size: 384 439 279 665 473 354 3204
Fragments arranged by size:
3204 665 473 439 384 354 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1751 2061 2602 3381 3514 3973 4182 1751
Size: 310 541 779 133 459 209 3367
Fragments arranged by size:
3367 779 541 459 310 209 133
TatI w'GTAC_w
Cuts at: 5313 5313
Size: 5798
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5315 5347 5315
Size: 32 5766
Tth111I GACn'n_nGTC
Cuts at: 1357 1660 1948 3595 1357
Size: 303 288 1647 3560
Fragments arranged by size:
3560 1647 303 288
XbaI T'CTAG_A
Cuts at: 2829 2829
Size: 5798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1599 3293 1599
Size: 1694 4104
XhoI C'TCGA_G
Cuts at: 1909 1909
Size: 5798
XmaI C'CCGG_G
Cuts at: 126 923 2688 2818 126
Size: 797 1765 130 3106
Fragments arranged by size:
3106 1765 797 130
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AscI AvrII BaeI BmgBI
BmtI BsiWI BsmBI BspEI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PmlI PsiI PsrI SacII
SanDI SexAI SnaBI SrfI SspI SwaI XmnI ZraI